中风解郁汤对卒中后抑郁模型大鼠行为学和炎症因子的影响
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摘要
目的:探讨中风解郁汤对卒中后抑郁(post-stroke depression,PSD)模型大鼠白细胞介素10(interleukin-10,IL-10)、IL-23与大鼠行为学的影响。方法:60只SD大鼠按照随机对照分组方法随机分为6组,分别为正常组、模型组、早期干预治疗组、进展期干预治疗组、早期对照组、进展期干预组,每组均为10只。应用改良的线栓法阻断SD大鼠的右侧大脑中动脉以制备梗死模型,再采用慢性不可预见的温和性应激及孤养法制备PSD大鼠模型,并在早期及进展期给予不同干预措施,早期界定于6 h以内,进展期界定于12 h～1周。治疗组给予中风解郁汤,对照组给予氟西汀胶囊,正常组、模型组不予任何处理,自由饮水、进食。14 d后,比较各组大鼠血清IL-10、IL-23水平,体质量,神经功能评分及行为学情况。结果:(1)神经评分方面:早期干预治疗组与进展期治疗组比较,差异有统计学意义(P=0. 043 <0. 05)。(2)行为学方面:早期干预治疗组水平穿越格数与进展期治疗组比较,差异有统计学意义(P=0. 02 <0. 05);早期干预治疗组站立次数与进展期治疗组比较,差异无统计学意义(P=0. 161> 0. 05)。(3)炎症因子:早期干预治疗组IL-10与进展期治疗组比较,差异有统计学意义(P=0. 000 <0. 01);早期对照组与进展期对照组比较,差异有统计学意义(P=0. 004 <0. 01);早期干预治疗组IL-23与进展期干预治疗组比较,差异有统计学意义(P=0. 005 <0. 01)。结论:中风解郁汤能改善PSD大鼠的神经功能评分及抑郁行为,可显著降低卒中后抑郁大鼠模型血清炎症因子的水平。
Objective: To explore the effects of Zhongfeng Jieyu Decoction on interleukin-10( IL-10),IL-23 and behavior of rats with post-stroke depression( PSD) model. Methods: 60 SD rats were randomly divided into six groups: normal group,model group,early intervention group,advanced intervention group,early control group and advanced intervention group. Each group consisted of10 rats. The right middle cerebral artery of SD rats was blocked by modified thread embolization to establish the infarction model.The PSD rat model was established by chronic unpredictable mild stress and solitary culture. Different interventions were given at the early and advanced stages,which were defined within 6 hours in the early stage and 12 hours to 1 week in the advanced stage.The treatment group was given Zhongfeng Jieyu Decoction,and the control group was given fluoxetine capsule. The normal group and the model group were not treated,and they were free to drink water and eat. After 14 days,serum IL-10,IL-23 levels,body mass,neurological function scores and behavioral status were compared between the groups. Results: ①Neurological score: There was significant difference between early intervention group and advanced treatment group( P = 0. 043 < 0. 05). ②There was a significant difference in the number of horizontal crossings between the early intervention group and the advanced treatment group( P = 0. 02 < 0. 05); there was no significant difference in the number of standing between the early intervention group and the advanced treatment group( P = 0. 161 > 0. 05). ③inflammatory factors: There was significant difference between the early intervention group and the advanced treatment group( P = 0. 000 < 0. 01); there was significant difference between the early control group and the advanced control group( P = 0. 004 < 0. 01); there was significant difference between the early intervention group and the advanced intervention group( P = 0. 005 < 0. 01). Conclusion: Zhongfeng Jieyu Decoction can improve the neurological function score and depressive behavior of PSD rats,and significantly reduce the level of serum inflammatory factors in post-stroke depression rats.
Objective: To explore the effects of Zhongfeng Jieyu Decoction on interleukin-10( IL-10),IL-23 and behavior of rats with post-stroke depression( PSD) model. Methods: 60 SD rats were randomly divided into six groups: normal group,model group,early intervention group,advanced intervention group,early control group and advanced intervention group. Each group consisted of10 rats. The right middle cerebral artery of SD rats was blocked by modified thread embolization to establish the infarction model.The PSD rat model was established by chronic unpredictable mild stress and solitary culture. Different interventions were given at the early and advanced stages,which were defined within 6 hours in the early stage and 12 hours to 1 week in the advanced stage.The treatment group was given Zhongfeng Jieyu Decoction,and the control group was given fluoxetine capsule. The normal group and the model group were not treated,and they were free to drink water and eat. After 14 days,serum IL-10,IL-23 levels,body mass,neurological function scores and behavioral status were compared between the groups. Results: ①Neurological score: There was significant difference between early intervention group and advanced treatment group( P = 0. 043 < 0. 05). ②There was a significant difference in the number of horizontal crossings between the early intervention group and the advanced treatment group( P = 0. 02 < 0. 05); there was no significant difference in the number of standing between the early intervention group and the advanced treatment group( P = 0. 161 > 0. 05). ③inflammatory factors: There was significant difference between the early intervention group and the advanced treatment group( P = 0. 000 < 0. 01); there was significant difference between the early control group and the advanced control group( P = 0. 004 < 0. 01); there was significant difference between the early intervention group and the advanced intervention group( P = 0. 005 < 0. 01). Conclusion: Zhongfeng Jieyu Decoction can improve the neurological function score and depressive behavior of PSD rats,and significantly reduce the level of serum inflammatory factors in post-stroke depression rats.
引文
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[2]李海华,汪毅,张琴,等.脑卒中后抑郁与神经功能缺损的关系研究[J].重庆医学,2012,41(3):241-243.
[3]杨玲俐,张志珺,孙鼎.脑卒中急性期患者卒中后抑郁的发生率及其危险因素[J].临床神经病学杂志,2010,23(3):185.
[4]姜炎,连亚军.鼠神经生长因子对急性脑梗死大鼠炎性因子与神经功能的影响[J].中华神经外科杂志,2016,23(7):1819-1821.
[5]沈晓明,韩宁,马云枝,等.舒郁颗粒对卒中后抑郁大鼠模型海马单胺类神经递质及炎症细胞因子的影响[J].中国实验方剂学杂志,2011,17(19):177-180.
[6]汪亮,刘潘潘,胡勤乐.线栓法致大鼠局灶性脑缺血I再灌注模型的制作及思考[J].医学综述,2011,17(21):3359-3361.
[7]李国前,王杰华,徐秀秀,等.瑞舒伐他汀对脑缺血再灌注大鼠白介素-23、白介素-17炎性轴的影响[J].中国临床药理学杂志,2016,32(10):894-896.
[8]封倩,冯来会,杨新年.加味酸枣仁汤对慢性应激抑郁模型大鼠脑组织单胺类神经递质NE、DA及5-HT影响的实验研究[J].中医学报,2012,27(5):581-582.
[9]郭晓冬,许二平,李琳,等.加味丹栀逍遥散对抑郁模型大鼠下丘脑内5-HT和NE的影响[J].中医学报,2013,28(12):1831-1833.
[10]范文涛,王倩,刘柏炎.地黄饮子对卒中后抑郁大鼠海马5-羟色胺受体的影响[J].中国实验方剂学杂志,2013,19(16):224-228.
[11]崔宁,李跃华,孙锦锦,等.抑郁症患者5-羟色胺与中医证型及症状关系分析[J].中国中医药信息杂志,2013,20(2):24-26.
[12]关东升,李迎霞,孟毅,等.针药干预卒中后抑郁症伴躯体形式障碍临床研究[J].中医学报,2013,28(5):763-764.
[13]李乐军,李鑫,黄蓉,等.舒郁胶囊对血管性抑郁模型大鼠脑组织5-HT1AR,D2R,α2AR及Apo E4表达的影响[J].中国实验方剂学杂志,2014,20(13):160-163.