芸薹属植物提取液对烤烟黑胫病发生及烟株生理特性的影响
|
摘要
采用平板试验和盆栽试验研究了3种芸薹属植物(芜菁、芥菜和油菜)提取液对烟草黑胫病菌丝生长、烟株黑胫病发生状况、烟株生长及生理特性的影响。结果表明:(1)与对照相比,3种芸薹属植物提取液均显著地抑制烟草黑胫病菌丝的生长,在最高浓度10 mg·m L~(-1)的条件下,芜菁、芥菜和油菜提取液对烟草黑胫病菌丝的抑制率分别为46.90%、52.41%和61.53%,以油菜提取液的抑菌效果最好。(2)与对照相比,不同芸薹属植物提取液均显著地降低烟草黑胫病的发病率和病情指数,防治效果为32.59%~76.21%。(3)与对照相比,3种芸薹属植物提取液均显著增加烟株的茎围、最大叶面积和株高,显著提高烟株叶片的叶绿素含量及净光合速率(P_n)、蒸腾速率(T_r)和气孔导度(G_s),显著增强烟株叶片中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、多酚氧化酶(PPO)和苯丙氨酸解氨酶(PAL)活性,显著降低丙二醛(MDA)含量,促进烟株的生长。(4)就3种芸薹属植物提取液对烤烟的抗病效应而言,以油菜提取液对提高烤烟抗性和抑制烟草黑胫病发生的效果最好。
The effects of three Brassica [rappini(B. rapa), mustard(B. juncea) and rape(B. campestris)] extracts on the growth of mycelium of Phytophthora parasitica var. nicotianae, occurrence of black shank disease, plant growth and physiological characteristics of flue-cured tobacco(Nicotiana tabacum cv. ‘Yuyan No.6') were investigated by petri-dish and pot experiments. The results showed that:(1) compared with the control, three Brassica extracts significantly inhibited the growth of mycelium of P. parasitica var. nicotianae, the inhibition rates at 10 mg·m L-1 with rappini, mustard and rape extracts were 46.90%, 52.41% and 61.53%, respectively. The strongest inhibition effect was found in rape extract treatment.(2) Compared with the control, three Brassica extracts also significantly decreased disease incidence and disease index of tobacco black shank, the control effects were found from 32.59% to 76.21%.(3) Compared with the control, three Brassica extracts significantly increased plant height, stem thick and maximum leaf area, remarkably improved chlorophyll content and photosynthetic performance of tobacco leaves, notably enhanced the activities of superoxide dismutase(SOD), catalase(CAT), peroxidase(POD), polyphenol oxidase(PPO), phenylalanine ammonia lyase(PAL), and significantly reduced malondiadehyde(MDA) content of tobacco leaves.(4) The rape extract played a better efficient role on inducing resistance of tobacco and controlling occurrence of tobacco black shank than rappini and mustard extracts.
The effects of three Brassica [rappini(B. rapa), mustard(B. juncea) and rape(B. campestris)] extracts on the growth of mycelium of Phytophthora parasitica var. nicotianae, occurrence of black shank disease, plant growth and physiological characteristics of flue-cured tobacco(Nicotiana tabacum cv. ‘Yuyan No.6') were investigated by petri-dish and pot experiments. The results showed that:(1) compared with the control, three Brassica extracts significantly inhibited the growth of mycelium of P. parasitica var. nicotianae, the inhibition rates at 10 mg·m L-1 with rappini, mustard and rape extracts were 46.90%, 52.41% and 61.53%, respectively. The strongest inhibition effect was found in rape extract treatment.(2) Compared with the control, three Brassica extracts also significantly decreased disease incidence and disease index of tobacco black shank, the control effects were found from 32.59% to 76.21%.(3) Compared with the control, three Brassica extracts significantly increased plant height, stem thick and maximum leaf area, remarkably improved chlorophyll content and photosynthetic performance of tobacco leaves, notably enhanced the activities of superoxide dismutase(SOD), catalase(CAT), peroxidase(POD), polyphenol oxidase(PPO), phenylalanine ammonia lyase(PAL), and significantly reduced malondiadehyde(MDA) content of tobacco leaves.(4) The rape extract played a better efficient role on inducing resistance of tobacco and controlling occurrence of tobacco black shank than rappini and mustard extracts.
引文
Bai HQ(2009).Effects of the plant source extracts on physiological-biochemical index and ultrastructure of tobacco plants infected by TMV(Master’s thesis).Zhengzhou:Henan Agricultural University(in Chinese with English abstract)[白海群(2009).植物提取液对感染TMV烟叶生理生化特性及超微结构的影响(硕士论文).郑州:河南农业大学]
Chen JX,Wang XF(2006).Experimental Guide of Physiology of Plant.2nd ed.Guangzhou:South China Science and Technology University Press(in Chinese)[陈建勋,王晓峰(2006).第2版.植物生理学实验指导.广州:华南理工大学出版社]
Chen XJ,Zhu ZJ,Yang J,Liu YH(2006).Composition and content of glucosinolates in leaves and bolting stems of Chinese kale quantified by HPLC.Acta Hortic Sin,33(4):741-744(in Chinese with English abstract)[陈新娟,朱祝军,杨静,刘永华(2006).芥蓝叶和薹的硫代葡萄糖苷组分及含量.园艺学报,33(4):741-744]
Geng K,Luo WF,Yang YL(2002).Population distribution of Phytophthora parasitica var.nicotianae in soil of tobacco field.JYunnan Agric Univ,17(4):389-392(in Chinese with English abstract)[耿坤,罗文富,杨艳丽(2002).烟草黑胫病菌的田间群体分布规律.云南农业大学学报,17(4):389-392]
He HJ,Chen H,Schnitzler WH(2002).Glucosinolate composition and contents in Brassica vegetables.Sci Agric Sin,35(2):192-197(in Chinese with English abstract)[何洪巨,陈杭,Schnitzler WH(2002).芸薹属蔬菜中硫代葡萄糖苷鉴定与含量分析.中国农业科学,35(2):192-197]
Lai RQ,Jiang LC,Chen ZM,Zhou DX,Zhou DJ(2009).Inhibitory effects of garlic extract on Phytophthora parasitica var nicotianae in laboratory.Tobac Sci Tech,(9):62-64(in Chinese with English abstract)[赖荣泉,姜林灿,陈志敏,周东新,周道金(2009).大蒜粗提物对烟草黑胫病菌的室内抑制作用.烟草科技,(9):62-64]
Larkin RP,Griffin TS(2007).Control of soilborne potato diseases using Brassica green manures.Crop Prot,26(7):1067-1077
Larkin RP,Honeycutt CW,Olanya OM(2011).Management of Verticillium wilt of potato with disease-suppressive green manures and as affected by previous cropping history.Plant Dis,95(5):568-576
Li F,Zhang CL(2006).The bio-control function of Brassica species.Chin J Oil Crop Sci,28(1):97-103(in Chinese with English abstract)[李锋,张春雷(2006).芸薹属植物的生防作用.中国油料作物学报,28(1):97-103]
Li F,Zhang CL,Li GM(2006).Composition and content of glucosinolates in rapeseed and in vitro inhibition of Botoytis cinerea persoon.J Wuhan Bot Res,24(4):351-356(in Chinese with English abstract)[李锋,张春雷,李光明(2006).油菜硫苷组分含量及抑菌活性研究.武汉植物学研究,24(4):351-356]
Li HS(2000).Technology and Elements of Experimentation in Plant Physiology and Chemistry.Beijing:Higher Education Press,167-169(in Chinese)[李合生(2000).植物生理生化实验原理和技术.北京:高等教育出版社,167-169]
Li JW,Zhou JX,Zhang XW,Zhao SM,Wang H,Wang XD,Liu L(2016).Growth and glucosinolate content of five Cruciferae plants during idle time of flue-cured tobacco production.Hubei Agric Sci,55(19):5056-5058,5132(in Chinese with English abstract)[李继伟,周俊学,张喜文,赵世民,王慧,王小东,刘领(2016).烤烟田休闲期5种绿肥的生物量及硫苷含量比较.湖北农业科学,55(19):5056-5058,5132]
Li MS,Li SD,Miu ZQ,Guo RJ,Zhao ZY(2006).Biofumigation for management of soilborne plant diseases.Chin J Biol Control,22(4):296-302(in Chinese with English abstract)[李明社,李世东,缪作清,郭荣君,赵震宇(2006).生物熏蒸用于植物土传病害治理的研究.中国生物防治,22(4):296-302]
Li PW,Zhao YG,Zhang W,Ding XX,Yang M,Wang XF,Xie CH,Fu YD(2005).Analysis of glucosinolate components and profiles in Brassica napus.Sci Agric Sin,38(7):1346-1352(in Chinese with English abstract)[李培武,赵永国,张文,丁小霞,杨湄,汪雪芳,谢从华,傅廷栋(2005).中国甘蓝型油菜硫苷含量及组份分析.中国农业科学,38(7):1346-1352]
Ling N,Xue C,Huang QW,Yang XM,Xu YC,Shen QR(2010).Development of a mode of application of bioorganic fertilizer for improving the biocontrol efficacy to Fusarium wilt.Bio Control,55:673-683
Liu HS,Han JF,Zhang YF,Meng FT,Wang F,Guo CB(2006).The inhibitory effects of extract from Basella rubral L.on tobacco mosaic virus.Acta Agric Boreali Sin,22(6):184-187(in Chinese with English abstract)[刘华山,韩锦峰,张玉丰,孟凡庭,王方,郭传滨(2006).落葵提取液抑制普通烟草花叶病毒病的生理效应研究.华北农学报,22(6):184-187]
Ma GS,Gao ZM,Chen J(2001).Recent research advance on tobacco black shank.Tobac Sci Tech,(9):44-48(in Chinese with English abstract)[马国胜,高智谋,陈娟(2001).烟草黑胫病研究进展.烟草科技,(9):44-48]
Njoroge SMC,Riley MB,Keinath AP(2008).Effect of incorporation of Brassica spp.residues on population densities of soilborne microorganisms and on damping-off and Fusarium wilt of watermelon.Plant Dis,92:287-294
Ping WL,Li XJ,Zhu JW,Guo KJ(2013).Research progress in integrated management strategies of tobacco black shank.J Henan Agric Sci,42(5):12-16(in Chinese with English abstract)[平文丽,李雪君,朱景伟,郭凯君(2013).烤烟黑胫病的防治策略研究进展.河南农业科学,42(5):12-16]
Qian LL,Liu JL,Li Y,Zhang YY,Pei SC(2008).Study on distilled glucosinolates and antimicrobial from broccoli.Chin Agric Sci Bull,24(2):335-338(in Chinese with English abstract)[钱丽丽,刘江丽,李扬,张园园,裴世春(2008).西兰花中硫代葡萄糖苷的提取及抑菌试验初报.中国农学通报,24(2):335-338]
Qiao SJ,Li SM,Meng LB(2010).Effect of Brassica plants suppressing four soilborne pathogenic microbes by biofumigation.J Northeast Agric Univ,41(5):19-24(in Chinese with English abstract)[乔世佳,李淑敏,孟令波(2010).芸薹属植物对四种土传病原微生物熏蒸效果的研究.东北农业大学学报,41(5):19-24]
Rao HY,Shao ZC,Liu HN,Wu YY,Liu R,Li XF,Li MQ,Qian PX(2015).Effect of browning inhibitors on callus subculture of phenolic compounds,enzyme and gene expression of grape.Plant Physiol J,51(8):1322-1330(in Chinese with English abstract)[饶慧云,邵祖超,柳海宁,吴月燕,刘蓉,李学孚,李美芹,钱萍仙(2015).抗褐化剂对葡萄愈伤组织继代培养过程中酚类物质、相关酶及其基因表达的影响.植物生理学报,51(8):1322-1330]
Song JY,Liu L,Chen MC,Li YJ,Bao XL(2014).Effects of biochar addition on growth and photosynthetic characteristics of fluecured tobacco.J Henan Univ Sci Tech(Nat Sci),35(4):68-72,8(in Chinese with English abstract)[宋久洋,刘领,陈明灿,李友军,暴秀丽(2014).生物质炭施用对烤烟生长及光合特性的影响.河南科技大学学报(自然科学版),35(4):68-72,8]
Sun WY,He HJ,Zhang HY,Zhang FS(2009).Components and concentration of glucosinolates in shoots and roots of different turnip cultivars.China Veg,(4):35-39(in Chinese with English abstract)[孙文彦,何洪巨,张宏彦,张福锁(2009).不同品种芜菁地上部和根部硫代葡萄糖苷组分及含量.中国蔬菜,(4):35-39]
Wan NF,Chen XQ,Ji XY,Jiang JX(2013).Sublethal effects of Angelica sinensis and Ricinus communis extracts on the growth,development and fecundity of Spodoptera exigua Hübner.Chin JEco-Agric,21(9):1135-1141(in Chinese with English abstract)[万年峰,陈晓勤,季香云,蒋杰贤(2013).当归和蓖麻乙醇提取液对甜菜夜蛾生长发育和繁殖的亚致死效应.中国生态农业学报,21(9):1135-1141]
Wang CJ,Wang SS,Zhao Y,Yang YJ,Wang RY(2015).Study on the inhibitory effect of the aqueous extract of a botanical mixture on tobacco black shank and its influence on soil characteristics.Chin Tobac Sci,36(6):83-87(in Chinese with English abstract)[王传吉,王树声,赵阳,杨银菊,王若焱(2015).一种植物源复配剂对烟草黑胫病的抑制及土壤特性的影响.中国烟草科学,36(6):83-87]
Wang DJ,Yang ZC,Qiao SJ,Kang CS,Yuan R,Yao XT,Hu XX,Li SM(2016).Effects of biofumigation on inhibition of cucumber Fusarium wilt,quality and yield of cucumber.Chin Agric Sci Bull,32(28):125-130(in Chinese with English abstract)[王德江,杨自超,乔世佳,康春生,袁睿,姚小桐,胡小璇,李淑敏(2016).生物熏蒸对黄瓜枯萎病抑制及品质和产量的影响.中国农学通报,32(28):125-130]
Wang HB,Shi J,Luo ZN,Hu YJ,Chen Q,Wang C(2016).Isolation and identification of Penicillium strain QMYCS-2 and its effect on tobacco black shank.Tobac Sci Tech,49(2):14-20(in Chinese with English abstract)[王海波,时焦,雒振宁,胡亚杰,陈强,王聪(2016).青霉菌QMYCS-2菌株的分离鉴定及其对烟草黑胫病的防治作用.烟草科技,49(2):14-20]
Wang HC,Li WH,Feng YG,Shi JX(2011).History and current status of chemical control of tobacco black shank.Acta Tabacaria Sin,17(5):96-102(in Chinese with English abstract)[汪汉成,李文红,冯勇刚,石俊雄(2011).烟草黑胫病化学防治的历史与现状.中国烟草学报,17(5):96-102]
Wang J,Kong FY,Zhang CS,Feng C(2013).Antagonism effect and enzyme producing comparison of Actinomycetes F8 against tobacco black shank.Chin Tobac Sci,34(2):49-53(in Chinese with English abstract)[王静,孔凡玉,张成省,冯超(2013).放线菌F8对烟草黑胫病的拮抗作用及其产酶活性.中国烟草科学,34(2):49-53]
Wu BQ,Liang YJ,Ding YQ,Chen XM,Wang YJ,Xu Z,Du BH(2013).Study on disease-preventing and growth-promoting effects of two antifungal bacteria from tobacco rhizosphere.Chin Tobac Sci,34(1):66-71(in Chinese with English abstract)[吴秉奇,梁永江,丁延芹,陈晓明,王玉军,徐峥,杜秉海(2013).两株烟草根际拮抗菌的生防和促生效果研究.中国烟草科学,34(1):66-71]
Xiao YS,Zeng WA,Zeng GQ,Li SY,Chen RZ,Yang YJ(2010).Control effect experiment of pesticide against tobacco black shank.Tobac Sci Tech,(7):62-64(in Chinese with English abstract)[肖艳松,曾维爱,曾广庆,李盛元,陈瑞忠,阳运即(2010).防治烟草黑胫病的药效试验.烟草科技,(7):62-64]
Xie YH,Zhang YG,Zhu LQ,You DG,Lu Y(2015).Research advances in integrated management of tobacco black shank.Curr Biotech,5(1):41-46(in Chinese with English abstract)[谢永辉,张永贵,朱利全,尤道贵,鲁耀(2015).烟草黑胫病综合防治研究进展.生物技术进展,5(1):41-46]
Zhan HX,Wen NN,Luo DQ,Xia JH,Xu CT,Zhang CS(2015).Races of Phytophthora nicotianae and their sensitivity to metalaxyl and dimethomorph in Luzhou,Sichuan.Plant Prot,41(6):178-184,190(in Chinese with English abstract)[战徊旭,温娜娜,罗定棋,夏建华,徐传涛,张成省(2015).泸州烟草黑胫病病原生理小种组成及对甲霜灵和烯酰吗啉的敏感性.植物保护,41(6):178-184,190]
Zhang K,Xie LL,Wu YJ,Zhang XQ,Yang TZ(2015).Research progress on occurrence of tobacco black shank and its integrated control.J Agric Sci Tech China,17(4):62-70(in Chinese with English abstract)[张凯,谢利丽,武云杰,张小全,杨铁钊(2015).烟草黑胫病的发生及综合防治研究进展.中国农业科技导报,17(4):62-70]
Zheng WY,Ding ZH,Liu H,Deng C,Xiao ZR(2013).Effect of pathogens causing discoloration on defensive system in red pepper(Capsicum annuum L.).Plant Physiol J,49(4):357-361(in Chinese with English abstract)[郑文宇,丁筑红,刘海,邓程,肖治柔(2013).“花壳”病菌对辣椒防御系统的影响.植物生理学报,49(4):357-361]
Zhu XM,Shi XP,Yong DJ,Zhang Y,Li BH,Liang WX,Wang CX(2015).Induction of resistance against Glomerella cingulata in apple by endophytic actinomycetes strain A-1.Plant Physiol J,51(6):949-954(in Chinese with English abstract).[朱学明,史祥鹏,雍道敬,张颖,李保华,梁文星,王彩霞(2015).内生放线菌A-1诱导苹果对炭疽叶枯病的抗性.植物生理学报,51(6):949-954]
Zhou XX,Zhou Q,Hu RS,Zhou JH(2011).Research advance in biological control of tobacco black shank.Acta Agric Jiangxi,23(7):124-126(in Chinese with English abstract)[周喜新,周倩,胡日生,周冀衡(2011).烟草黑胫病生物防治研究进展.江西农业学报,23(7):124-126]
Chen JX,Wang XF(2006).Experimental Guide of Physiology of Plant.2nd ed.Guangzhou:South China Science and Technology University Press(in Chinese)[陈建勋,王晓峰(2006).第2版.植物生理学实验指导.广州:华南理工大学出版社]
Chen XJ,Zhu ZJ,Yang J,Liu YH(2006).Composition and content of glucosinolates in leaves and bolting stems of Chinese kale quantified by HPLC.Acta Hortic Sin,33(4):741-744(in Chinese with English abstract)[陈新娟,朱祝军,杨静,刘永华(2006).芥蓝叶和薹的硫代葡萄糖苷组分及含量.园艺学报,33(4):741-744]
Geng K,Luo WF,Yang YL(2002).Population distribution of Phytophthora parasitica var.nicotianae in soil of tobacco field.JYunnan Agric Univ,17(4):389-392(in Chinese with English abstract)[耿坤,罗文富,杨艳丽(2002).烟草黑胫病菌的田间群体分布规律.云南农业大学学报,17(4):389-392]
He HJ,Chen H,Schnitzler WH(2002).Glucosinolate composition and contents in Brassica vegetables.Sci Agric Sin,35(2):192-197(in Chinese with English abstract)[何洪巨,陈杭,Schnitzler WH(2002).芸薹属蔬菜中硫代葡萄糖苷鉴定与含量分析.中国农业科学,35(2):192-197]
Lai RQ,Jiang LC,Chen ZM,Zhou DX,Zhou DJ(2009).Inhibitory effects of garlic extract on Phytophthora parasitica var nicotianae in laboratory.Tobac Sci Tech,(9):62-64(in Chinese with English abstract)[赖荣泉,姜林灿,陈志敏,周东新,周道金(2009).大蒜粗提物对烟草黑胫病菌的室内抑制作用.烟草科技,(9):62-64]
Larkin RP,Griffin TS(2007).Control of soilborne potato diseases using Brassica green manures.Crop Prot,26(7):1067-1077
Larkin RP,Honeycutt CW,Olanya OM(2011).Management of Verticillium wilt of potato with disease-suppressive green manures and as affected by previous cropping history.Plant Dis,95(5):568-576
Li F,Zhang CL(2006).The bio-control function of Brassica species.Chin J Oil Crop Sci,28(1):97-103(in Chinese with English abstract)[李锋,张春雷(2006).芸薹属植物的生防作用.中国油料作物学报,28(1):97-103]
Li F,Zhang CL,Li GM(2006).Composition and content of glucosinolates in rapeseed and in vitro inhibition of Botoytis cinerea persoon.J Wuhan Bot Res,24(4):351-356(in Chinese with English abstract)[李锋,张春雷,李光明(2006).油菜硫苷组分含量及抑菌活性研究.武汉植物学研究,24(4):351-356]
Li HS(2000).Technology and Elements of Experimentation in Plant Physiology and Chemistry.Beijing:Higher Education Press,167-169(in Chinese)[李合生(2000).植物生理生化实验原理和技术.北京:高等教育出版社,167-169]
Li JW,Zhou JX,Zhang XW,Zhao SM,Wang H,Wang XD,Liu L(2016).Growth and glucosinolate content of five Cruciferae plants during idle time of flue-cured tobacco production.Hubei Agric Sci,55(19):5056-5058,5132(in Chinese with English abstract)[李继伟,周俊学,张喜文,赵世民,王慧,王小东,刘领(2016).烤烟田休闲期5种绿肥的生物量及硫苷含量比较.湖北农业科学,55(19):5056-5058,5132]
Li MS,Li SD,Miu ZQ,Guo RJ,Zhao ZY(2006).Biofumigation for management of soilborne plant diseases.Chin J Biol Control,22(4):296-302(in Chinese with English abstract)[李明社,李世东,缪作清,郭荣君,赵震宇(2006).生物熏蒸用于植物土传病害治理的研究.中国生物防治,22(4):296-302]
Li PW,Zhao YG,Zhang W,Ding XX,Yang M,Wang XF,Xie CH,Fu YD(2005).Analysis of glucosinolate components and profiles in Brassica napus.Sci Agric Sin,38(7):1346-1352(in Chinese with English abstract)[李培武,赵永国,张文,丁小霞,杨湄,汪雪芳,谢从华,傅廷栋(2005).中国甘蓝型油菜硫苷含量及组份分析.中国农业科学,38(7):1346-1352]
Ling N,Xue C,Huang QW,Yang XM,Xu YC,Shen QR(2010).Development of a mode of application of bioorganic fertilizer for improving the biocontrol efficacy to Fusarium wilt.Bio Control,55:673-683
Liu HS,Han JF,Zhang YF,Meng FT,Wang F,Guo CB(2006).The inhibitory effects of extract from Basella rubral L.on tobacco mosaic virus.Acta Agric Boreali Sin,22(6):184-187(in Chinese with English abstract)[刘华山,韩锦峰,张玉丰,孟凡庭,王方,郭传滨(2006).落葵提取液抑制普通烟草花叶病毒病的生理效应研究.华北农学报,22(6):184-187]
Ma GS,Gao ZM,Chen J(2001).Recent research advance on tobacco black shank.Tobac Sci Tech,(9):44-48(in Chinese with English abstract)[马国胜,高智谋,陈娟(2001).烟草黑胫病研究进展.烟草科技,(9):44-48]
Njoroge SMC,Riley MB,Keinath AP(2008).Effect of incorporation of Brassica spp.residues on population densities of soilborne microorganisms and on damping-off and Fusarium wilt of watermelon.Plant Dis,92:287-294
Ping WL,Li XJ,Zhu JW,Guo KJ(2013).Research progress in integrated management strategies of tobacco black shank.J Henan Agric Sci,42(5):12-16(in Chinese with English abstract)[平文丽,李雪君,朱景伟,郭凯君(2013).烤烟黑胫病的防治策略研究进展.河南农业科学,42(5):12-16]
Qian LL,Liu JL,Li Y,Zhang YY,Pei SC(2008).Study on distilled glucosinolates and antimicrobial from broccoli.Chin Agric Sci Bull,24(2):335-338(in Chinese with English abstract)[钱丽丽,刘江丽,李扬,张园园,裴世春(2008).西兰花中硫代葡萄糖苷的提取及抑菌试验初报.中国农学通报,24(2):335-338]
Qiao SJ,Li SM,Meng LB(2010).Effect of Brassica plants suppressing four soilborne pathogenic microbes by biofumigation.J Northeast Agric Univ,41(5):19-24(in Chinese with English abstract)[乔世佳,李淑敏,孟令波(2010).芸薹属植物对四种土传病原微生物熏蒸效果的研究.东北农业大学学报,41(5):19-24]
Rao HY,Shao ZC,Liu HN,Wu YY,Liu R,Li XF,Li MQ,Qian PX(2015).Effect of browning inhibitors on callus subculture of phenolic compounds,enzyme and gene expression of grape.Plant Physiol J,51(8):1322-1330(in Chinese with English abstract)[饶慧云,邵祖超,柳海宁,吴月燕,刘蓉,李学孚,李美芹,钱萍仙(2015).抗褐化剂对葡萄愈伤组织继代培养过程中酚类物质、相关酶及其基因表达的影响.植物生理学报,51(8):1322-1330]
Song JY,Liu L,Chen MC,Li YJ,Bao XL(2014).Effects of biochar addition on growth and photosynthetic characteristics of fluecured tobacco.J Henan Univ Sci Tech(Nat Sci),35(4):68-72,8(in Chinese with English abstract)[宋久洋,刘领,陈明灿,李友军,暴秀丽(2014).生物质炭施用对烤烟生长及光合特性的影响.河南科技大学学报(自然科学版),35(4):68-72,8]
Sun WY,He HJ,Zhang HY,Zhang FS(2009).Components and concentration of glucosinolates in shoots and roots of different turnip cultivars.China Veg,(4):35-39(in Chinese with English abstract)[孙文彦,何洪巨,张宏彦,张福锁(2009).不同品种芜菁地上部和根部硫代葡萄糖苷组分及含量.中国蔬菜,(4):35-39]
Wan NF,Chen XQ,Ji XY,Jiang JX(2013).Sublethal effects of Angelica sinensis and Ricinus communis extracts on the growth,development and fecundity of Spodoptera exigua Hübner.Chin JEco-Agric,21(9):1135-1141(in Chinese with English abstract)[万年峰,陈晓勤,季香云,蒋杰贤(2013).当归和蓖麻乙醇提取液对甜菜夜蛾生长发育和繁殖的亚致死效应.中国生态农业学报,21(9):1135-1141]
Wang CJ,Wang SS,Zhao Y,Yang YJ,Wang RY(2015).Study on the inhibitory effect of the aqueous extract of a botanical mixture on tobacco black shank and its influence on soil characteristics.Chin Tobac Sci,36(6):83-87(in Chinese with English abstract)[王传吉,王树声,赵阳,杨银菊,王若焱(2015).一种植物源复配剂对烟草黑胫病的抑制及土壤特性的影响.中国烟草科学,36(6):83-87]
Wang DJ,Yang ZC,Qiao SJ,Kang CS,Yuan R,Yao XT,Hu XX,Li SM(2016).Effects of biofumigation on inhibition of cucumber Fusarium wilt,quality and yield of cucumber.Chin Agric Sci Bull,32(28):125-130(in Chinese with English abstract)[王德江,杨自超,乔世佳,康春生,袁睿,姚小桐,胡小璇,李淑敏(2016).生物熏蒸对黄瓜枯萎病抑制及品质和产量的影响.中国农学通报,32(28):125-130]
Wang HB,Shi J,Luo ZN,Hu YJ,Chen Q,Wang C(2016).Isolation and identification of Penicillium strain QMYCS-2 and its effect on tobacco black shank.Tobac Sci Tech,49(2):14-20(in Chinese with English abstract)[王海波,时焦,雒振宁,胡亚杰,陈强,王聪(2016).青霉菌QMYCS-2菌株的分离鉴定及其对烟草黑胫病的防治作用.烟草科技,49(2):14-20]
Wang HC,Li WH,Feng YG,Shi JX(2011).History and current status of chemical control of tobacco black shank.Acta Tabacaria Sin,17(5):96-102(in Chinese with English abstract)[汪汉成,李文红,冯勇刚,石俊雄(2011).烟草黑胫病化学防治的历史与现状.中国烟草学报,17(5):96-102]
Wang J,Kong FY,Zhang CS,Feng C(2013).Antagonism effect and enzyme producing comparison of Actinomycetes F8 against tobacco black shank.Chin Tobac Sci,34(2):49-53(in Chinese with English abstract)[王静,孔凡玉,张成省,冯超(2013).放线菌F8对烟草黑胫病的拮抗作用及其产酶活性.中国烟草科学,34(2):49-53]
Wu BQ,Liang YJ,Ding YQ,Chen XM,Wang YJ,Xu Z,Du BH(2013).Study on disease-preventing and growth-promoting effects of two antifungal bacteria from tobacco rhizosphere.Chin Tobac Sci,34(1):66-71(in Chinese with English abstract)[吴秉奇,梁永江,丁延芹,陈晓明,王玉军,徐峥,杜秉海(2013).两株烟草根际拮抗菌的生防和促生效果研究.中国烟草科学,34(1):66-71]
Xiao YS,Zeng WA,Zeng GQ,Li SY,Chen RZ,Yang YJ(2010).Control effect experiment of pesticide against tobacco black shank.Tobac Sci Tech,(7):62-64(in Chinese with English abstract)[肖艳松,曾维爱,曾广庆,李盛元,陈瑞忠,阳运即(2010).防治烟草黑胫病的药效试验.烟草科技,(7):62-64]
Xie YH,Zhang YG,Zhu LQ,You DG,Lu Y(2015).Research advances in integrated management of tobacco black shank.Curr Biotech,5(1):41-46(in Chinese with English abstract)[谢永辉,张永贵,朱利全,尤道贵,鲁耀(2015).烟草黑胫病综合防治研究进展.生物技术进展,5(1):41-46]
Zhan HX,Wen NN,Luo DQ,Xia JH,Xu CT,Zhang CS(2015).Races of Phytophthora nicotianae and their sensitivity to metalaxyl and dimethomorph in Luzhou,Sichuan.Plant Prot,41(6):178-184,190(in Chinese with English abstract)[战徊旭,温娜娜,罗定棋,夏建华,徐传涛,张成省(2015).泸州烟草黑胫病病原生理小种组成及对甲霜灵和烯酰吗啉的敏感性.植物保护,41(6):178-184,190]
Zhang K,Xie LL,Wu YJ,Zhang XQ,Yang TZ(2015).Research progress on occurrence of tobacco black shank and its integrated control.J Agric Sci Tech China,17(4):62-70(in Chinese with English abstract)[张凯,谢利丽,武云杰,张小全,杨铁钊(2015).烟草黑胫病的发生及综合防治研究进展.中国农业科技导报,17(4):62-70]
Zheng WY,Ding ZH,Liu H,Deng C,Xiao ZR(2013).Effect of pathogens causing discoloration on defensive system in red pepper(Capsicum annuum L.).Plant Physiol J,49(4):357-361(in Chinese with English abstract)[郑文宇,丁筑红,刘海,邓程,肖治柔(2013).“花壳”病菌对辣椒防御系统的影响.植物生理学报,49(4):357-361]
Zhu XM,Shi XP,Yong DJ,Zhang Y,Li BH,Liang WX,Wang CX(2015).Induction of resistance against Glomerella cingulata in apple by endophytic actinomycetes strain A-1.Plant Physiol J,51(6):949-954(in Chinese with English abstract).[朱学明,史祥鹏,雍道敬,张颖,李保华,梁文星,王彩霞(2015).内生放线菌A-1诱导苹果对炭疽叶枯病的抗性.植物生理学报,51(6):949-954]
Zhou XX,Zhou Q,Hu RS,Zhou JH(2011).Research advance in biological control of tobacco black shank.Acta Agric Jiangxi,23(7):124-126(in Chinese with English abstract)[周喜新,周倩,胡日生,周冀衡(2011).烟草黑胫病生物防治研究进展.江西农业学报,23(7):124-126]