摘要
目的探讨甲胎蛋白(alpha-fetoprotein,AFP)与维甲酸受体(retinoic acid receptor,RAR)之间的相互作用。方法采用免疫荧光、荧光能量共振转移(fluorescence resonance energy transfer,Fret)及免疫共沉淀实验验证肝癌细胞系Hep G2和SMMC-7721胞质中AFP与RAR之间的相互关系。结果在胞质中AFP与RAR存在共定位现象,Fret结果显示,两者之间存在很高的能量转移效率,分别为21%和19%。免疫共沉淀实验结果显示,胞质中AFP与RAR存在相互作用。结论 AFP与RAR存在相互作用,为后续研究AFP的生物学功能,以及维甲酸的药物疗效提供实验基础。
Objective To study the interaction of AFP and RAR. Methods In this study, hepatocellular carcinoma cells Hep G2 and SMMC-7721 were analyzed by confocal microscopy, fluorescence resonance energy transfer(Fret) and co-immunoprecipitation(Co IP) to verify the relationship of AFP and RAR. Results Confocal microscopy results showed that AFP co-localized with RAR in cytoplasm. And Fret showed that high energy transfer efficiency existed between AFP and RAR in Hep G2(21%) and SMMC-7721(19%), indicating these two proteins might be interacted with each other.Subsequent Co IP experimental results confirmed that in cytoplasm AFP could interact with RAR. Conclusion It is confirmed that the AFP could interact with RAR. And it has laid a solid foundation for the future study of AFP biological function, and for the retinoic acid drug efficacy study.
引文
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