蛇皮胶原蛋白的提取工艺及优化
|
摘要
以蛇皮为原料,以胶原蛋白提取率为指标,采用高温酸法提取胶原蛋白。研究了有机酸种类及柠檬酸浓度、提取温度、浸提时间和固液比5个因素对蛇皮胶原蛋白提取率的影响。以单因素试验为基础,通过正交试验优化了蛇皮胶原蛋白的提取工艺。确定酸法提取蛇皮中胶原蛋白的较佳工艺为:以1 mol/L的柠檬酸作为浸提剂,采用1∶60(g∶m L)的固液比,在110℃下提取3 h,此时提取率较高,为87.01%。
Taking snake skin as raw material,and the extraction rate of collagen as the index,the high temperature acid method was used to extract collagen. The influences of the organic acid types and the concentration of citric acid,extraction temperature,extraction time and solid-to-liquid ratio on the extraction rate of collagen were studied. Based on single factor experiment,the extraction process of collagen of snake skin was optimized by orthogonal test. The optimized extraction process was obtained by acid extraction method which is found at the conditions of 1 mol/L initial concentration of citric acid as extraction agent with the solid-to-liquid ratio of 1∶ 60( g∶ m L),at a temperature of 110 ℃ of 3 h,the extraction rate is 87. 01%.
Taking snake skin as raw material,and the extraction rate of collagen as the index,the high temperature acid method was used to extract collagen. The influences of the organic acid types and the concentration of citric acid,extraction temperature,extraction time and solid-to-liquid ratio on the extraction rate of collagen were studied. Based on single factor experiment,the extraction process of collagen of snake skin was optimized by orthogonal test. The optimized extraction process was obtained by acid extraction method which is found at the conditions of 1 mol/L initial concentration of citric acid as extraction agent with the solid-to-liquid ratio of 1∶ 60( g∶ m L),at a temperature of 110 ℃ of 3 h,the extraction rate is 87. 01%.
引文
[1]陈毅军,刘正芹.胶原蛋白提取及其纺丝工艺研究现状[J].山东纺织科技,2016,57(5):54-56.
[2]王学川,任龙芳,强涛涛,等.胶原蛋白的研究进展及其在化妆品中的应用[J].日用化学工业,2005,35(6):388-392.
[3]卫功庆,刘少华,陈大勇,等.鹿产品中胶原蛋白的研究及应用进展[J].经济动物学报,2014,18(2):116-120.
[4]朱晓妮.胶原蛋白在食品中的应用现状及其发展前景分析[J].现代食品,2016(14):11-12.
[5]刘丽娜.水产动物胶原蛋白研究现状及发展前景[J].农产品加工学刊,2014(18):58-59,63.
[6]吴玉琼,骆红霞,胡永乐,等.木瓜蛋白酶提取蛇皮胶原蛋白的工艺研究[J].武夷学院学报,2015,34(6):26-30.
[7]李梁.东北林蛙皮胶原蛋白理化性质及胶原多肽活性研究[D].长春:吉林农业大学,2016.
[8]卓素珍.鮟鱇鱼皮胶原蛋白的性质、组成及应用研究[D].杭州:浙江工商大学,2009.
[9]王译伟,陈治梁.胶原蛋白提取工艺和临床应用进展[J].绿色科技,2016(2):180-182.
[10]陈丽丽,赵利,刘华,等.有机酸提取鮰鱼皮胶原蛋白的工艺研究[J].食品与机械,2010,26(5):118-121.
[11]俞艺萍,陆利霞,熊晓辉.鱼皮胶原蛋白提取研究进展[J].食品研究与开发,2010,31(12):262-265.
[12]孙宪迅,孙齐英,韩雪,等.罗非鱼鳞胶原蛋白提取工艺研究[J].江汉大学学报(自然科学版),2012,40(3):105-107.
[13]傅燕凤,沈月新,杨承刚,等.淡水鱼鱼皮胶原蛋白的提取[J].上海水产大学学报,2004(2):146-150.
[14]张培丽,周爱梅,刘欣,等.淡水鱼皮胶原蛋白提取工艺研究(Ⅰ)[J].食品与发酵工业,2006,32(12):150-153.
[15]赵燕燕,刘丽艳,杜光玲,等.高效液相色谱-蒸发光散射法测定奶制品中动物水解蛋白[J].食品科学,2010,31(20):282-285.
[16]王新雨,谭晓梅,陈飞龙,等.HPLC-ELSD法测定醋龟甲中羟脯氨酸[J].中草药,2011,42(7):1338-1340.
[2]王学川,任龙芳,强涛涛,等.胶原蛋白的研究进展及其在化妆品中的应用[J].日用化学工业,2005,35(6):388-392.
[3]卫功庆,刘少华,陈大勇,等.鹿产品中胶原蛋白的研究及应用进展[J].经济动物学报,2014,18(2):116-120.
[4]朱晓妮.胶原蛋白在食品中的应用现状及其发展前景分析[J].现代食品,2016(14):11-12.
[5]刘丽娜.水产动物胶原蛋白研究现状及发展前景[J].农产品加工学刊,2014(18):58-59,63.
[6]吴玉琼,骆红霞,胡永乐,等.木瓜蛋白酶提取蛇皮胶原蛋白的工艺研究[J].武夷学院学报,2015,34(6):26-30.
[7]李梁.东北林蛙皮胶原蛋白理化性质及胶原多肽活性研究[D].长春:吉林农业大学,2016.
[8]卓素珍.鮟鱇鱼皮胶原蛋白的性质、组成及应用研究[D].杭州:浙江工商大学,2009.
[9]王译伟,陈治梁.胶原蛋白提取工艺和临床应用进展[J].绿色科技,2016(2):180-182.
[10]陈丽丽,赵利,刘华,等.有机酸提取鮰鱼皮胶原蛋白的工艺研究[J].食品与机械,2010,26(5):118-121.
[11]俞艺萍,陆利霞,熊晓辉.鱼皮胶原蛋白提取研究进展[J].食品研究与开发,2010,31(12):262-265.
[12]孙宪迅,孙齐英,韩雪,等.罗非鱼鳞胶原蛋白提取工艺研究[J].江汉大学学报(自然科学版),2012,40(3):105-107.
[13]傅燕凤,沈月新,杨承刚,等.淡水鱼鱼皮胶原蛋白的提取[J].上海水产大学学报,2004(2):146-150.
[14]张培丽,周爱梅,刘欣,等.淡水鱼皮胶原蛋白提取工艺研究(Ⅰ)[J].食品与发酵工业,2006,32(12):150-153.
[15]赵燕燕,刘丽艳,杜光玲,等.高效液相色谱-蒸发光散射法测定奶制品中动物水解蛋白[J].食品科学,2010,31(20):282-285.
[16]王新雨,谭晓梅,陈飞龙,等.HPLC-ELSD法测定醋龟甲中羟脯氨酸[J].中草药,2011,42(7):1338-1340.