气相色谱法测定黔产夜寒苏挥发油及其桉油精含量
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摘要
目的:检测贵州不同产区夜寒苏挥发油的含量,并建立测定夜寒苏挥发油中桉油精含量的气相色谱方法,为黔产夜寒苏的质量评价提供理论依据。方法:采用水蒸气蒸馏法对夜寒苏药材进行挥发油的提取,用GC法对夜寒苏中所提取的桉油精进行含量测定。结果:贵州不同产区夜寒苏挥发油及桉油精含量差别较大;桉油精在0.157~5.025mg范围内与峰面积呈良好线性关系(r>0.999 8),平均加样回收率(n=6)为100.27%,RSD=2.11%。结论:该方法能够简便、准确、快速测定夜寒苏中挥发油和挥发油中桉油精的含量,可作为黔产夜寒苏的质量评价依据。
Objective:To detect the contents of the volatile oil of different regions in Guizhou Rhizoma Hedychium flavum and establish the determination method of eucalyptol in Rhizoma Hedychium flavum volatile oil by gas chromatography,which were providing theory basis for quality evaluation of Guizhou Rhizoma Hedychium flavum.Methods:The essential oil was extracted from Rhizoma Hedychium flavum by steam distillation.The content of eucalyptol in Rhizoma Hedychium flavum was measured by GC.Results:There is much difference in the content of volatile oil and eucalyptol of Guizhou Rhizoma Hedychium flavum from different regions of Guizhou province.Eucalyptol in the range of 0.157~5.025 mg showed good linear relationship(r>0.999 8).Average sample recovery rate(n=6)was 100.27%,RSD =2.11%.Conclusion:The method is simple,accurate and rapid to measure the content of volatile oil and eucalyptol from Guizhou Rhizoma Hedychium flavum,and it is suitable for the quality control of Guizhou Rhizoma Hedychium flavum.
Objective:To detect the contents of the volatile oil of different regions in Guizhou Rhizoma Hedychium flavum and establish the determination method of eucalyptol in Rhizoma Hedychium flavum volatile oil by gas chromatography,which were providing theory basis for quality evaluation of Guizhou Rhizoma Hedychium flavum.Methods:The essential oil was extracted from Rhizoma Hedychium flavum by steam distillation.The content of eucalyptol in Rhizoma Hedychium flavum was measured by GC.Results:There is much difference in the content of volatile oil and eucalyptol of Guizhou Rhizoma Hedychium flavum from different regions of Guizhou province.Eucalyptol in the range of 0.157~5.025 mg showed good linear relationship(r>0.999 8).Average sample recovery rate(n=6)was 100.27%,RSD =2.11%.Conclusion:The method is simple,accurate and rapid to measure the content of volatile oil and eucalyptol from Guizhou Rhizoma Hedychium flavum,and it is suitable for the quality control of Guizhou Rhizoma Hedychium flavum.
引文
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[6]赵晓頔,陈晓辉,谭晓婧,等.GC同时测定高良姜挥发油中α-蒎烯、β-蒎烯、桉油精和α-松油醇的含量[J].中国中药杂志,2009,34(21):2751-2753.
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[10]徐巧萍,王砚,唐法娣,等.1,8-桉油精对卵白蛋白致哮喘豚鼠的气道高反应性和炎症的抑制作用[J].中国药理学与毒理学杂志,2010,24(1):35-43.
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[12]宋灏,李姿,严启新.不同产地益智仁桉油精含量测定[J].河北中医,2011,33(3):426-427.
[13]王玉兰,于黎明,任玮,等.气相色谱法测定草果挥发油中桉油精的含量[J].时珍国医国药,2004,15(12):822-823.
[14]冯旭,梁臣艳,牛晋英,等.不同产地白豆蔻挥发油成分的GC-MS分析[J].中国试验方剂学杂志,2013,19(16):107-110.
[15]付琛,周日水,周光雄,不同产地品种阳春砂仁挥发油化学成分的气相色谱-质谱联用分析[J].时珍国医国药,2010,21(10):2534-2535.
[16]国家药典委员会.中华人民共和国药典(四部)[S].北京:中国医药科技出版,2015:203.
[2]胡秀,刘念.中国姜花属Hedychium野生花卉资源特点[J].广东园林,2009,31(4):7-11
[3]贵州省科学技术委员会.贵州植物志(第四卷)[M].成都:四川民族出社,1989:686-687.
[4]贵州省中医研究所.贵州草药(第二集)[M].贵阳:贵州人民出版社,1970:671.
[5]周汉华,赵曦,梁晓乐.夜寒苏的鉴定及挥发油成分GC-MS分析[J].中药材,2008,31(7):977-979.
[6]赵晓頔,陈晓辉,谭晓婧,等.GC同时测定高良姜挥发油中α-蒎烯、β-蒎烯、桉油精和α-松油醇的含量[J].中国中药杂志,2009,34(21):2751-2753.
[7]杨秀泽,周汉华,童红.夜寒苏(圆瓣姜花)的鉴定及挥发油成分GC-MS分析[J].中国药房,2011,22(7):642-643.
[8]马兴苗,周静,范玲,等.桉油精对补骨脂素体外抗肿瘤活性的增效作用研究[J].中成药,2013,35(5):903-908.
[9]张丽佳,薛银,胡松华,等.桉油精的抗菌抗炎作用研究[J].中国兽药杂志,2013,47(3):21-24.
[10]徐巧萍,王砚,唐法娣,等.1,8-桉油精对卵白蛋白致哮喘豚鼠的气道高反应性和炎症的抑制作用[J].中国药理学与毒理学杂志,2010,24(1):35-43.
[11]赖展鹏,程轩轩,杨全,等.不同种源高良姜1,8-桉油精含量及挥发油成分分析[J].亚太传统医药,2010,6(9):6-8.
[12]宋灏,李姿,严启新.不同产地益智仁桉油精含量测定[J].河北中医,2011,33(3):426-427.
[13]王玉兰,于黎明,任玮,等.气相色谱法测定草果挥发油中桉油精的含量[J].时珍国医国药,2004,15(12):822-823.
[14]冯旭,梁臣艳,牛晋英,等.不同产地白豆蔻挥发油成分的GC-MS分析[J].中国试验方剂学杂志,2013,19(16):107-110.
[15]付琛,周日水,周光雄,不同产地品种阳春砂仁挥发油化学成分的气相色谱-质谱联用分析[J].时珍国医国药,2010,21(10):2534-2535.
[16]国家药典委员会.中华人民共和国药典(四部)[S].北京:中国医药科技出版,2015:203.