培育年限对刺五加主要药用活性成分的影响
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摘要
为评价不同培育年限对刺五加根、茎部多种活性成分积累的共同影响,对提高刺五加培育和高值利用具有重要作用,可为规范化种植和合理开发利用刺五加提供理论依据。采集同一产地三年生、五年生、九年生刺五加为实验样本,运用超高效液相色谱系统(Ultra-Performance LC,Waters,Japan)分析不同培育年限对刺五加根、茎中槲皮苷、金丝桃苷、芦丁、紫丁香苷、刺五加苷E、异嗪皮啶6种活性成分含量的同时影响。结果表明,五年生刺五加根、茎中6种主要活性成分的综合得分最高。其中,黄酮类成分槲皮苷在三年生根中含量最高,在五年生茎中含量最高;芦丁在五年生根及茎中含量均最高,在根中三年生含量最低,九年生茎中含量最低;金丝桃苷在五年生根及三年生茎中含量最高。苯丙素类成分异嗪皮啶在三年生根及茎中含量最高,在五年生根和茎中含量最低;紫丁香苷在九年生根中含量最高,在五年茎中含量最高;刺五加E在五年生根中含量最高,在三年生茎中含量最高。不同药用成分在不同生长阶段的刺五加根和茎中积累不同,定向培育可根据目的活性成分选择适合的采收年限。
The common influence of different cultivation years on the accumulation of various active components in the root and stem of Acanthopanax senticosus was evaluated. It was important for raising the cultivation and high value utilization of A. senticosus. It provide a theoretical basis for standardized planting and rational exploitation and utilization of A. senticosus. The A. senticosus of different growth stage was collected from the same origin,and the Ultra-Performance LC(Waters,Japan) was used to analyze different growth stages of six secondary metabolites in the A. senticosus roots and stems. The total score of six main active components in root and stem of A. senticosus was the highest in five years. Among them,quercetin has the highest content in threeyear rooting and the highest content in five-year stem; rutin has the highest content in five year roots and stems,the lowest in three-years in root and lowest in nine-year stem; Hyperoside is the highest in five-year root and three-year stem. The content of isofraxidin in three year root and stem is the highest,and the content of root and stem is the lowest in five years. The content of syringin is the highest in nine-year root and in five-year stem.The highest content of eleutheroside e in the five-year rooting is the highest in the three-year stem. Different medicinal ingredients accumulated differently in roots and stems of A. senticosus in different cultivation years and targeted cultivation could select suitable harvest years according to the active components.
The common influence of different cultivation years on the accumulation of various active components in the root and stem of Acanthopanax senticosus was evaluated. It was important for raising the cultivation and high value utilization of A. senticosus. It provide a theoretical basis for standardized planting and rational exploitation and utilization of A. senticosus. The A. senticosus of different growth stage was collected from the same origin,and the Ultra-Performance LC(Waters,Japan) was used to analyze different growth stages of six secondary metabolites in the A. senticosus roots and stems. The total score of six main active components in root and stem of A. senticosus was the highest in five years. Among them,quercetin has the highest content in threeyear rooting and the highest content in five-year stem; rutin has the highest content in five year roots and stems,the lowest in three-years in root and lowest in nine-year stem; Hyperoside is the highest in five-year root and three-year stem. The content of isofraxidin in three year root and stem is the highest,and the content of root and stem is the lowest in five years. The content of syringin is the highest in nine-year root and in five-year stem.The highest content of eleutheroside e in the five-year rooting is the highest in the three-year stem. Different medicinal ingredients accumulated differently in roots and stems of A. senticosus in different cultivation years and targeted cultivation could select suitable harvest years according to the active components.
引文
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15.徐颀.蒸制轮叶党参的化学成分分析及药理活性研究[D].长春:吉林农业大学,2016.Xu Q.Study on the chemical composition and biological activity of steamed Codonopsis lanceolata[D].Changchun:Jilin Agricultural University,2016.
16.Ahn J,Um M Y,Lee H,et al.eleutheroside E,an active component of Eleutherococcus senticosus,ameliorates insulin resistance in type 2 diabetic db/db mice[J].EvidenceBased Complementary and Alternative Medicine,2013,2013:934183.
17.Huang L Z,Wei L,Zhao H F,et al.The effect of eleutheroside E on behavioral alterations in murine sleep deprivation stress model[J].European Journal of Pharmacology,2011,658(2-3):150-155.
18.汪洋,翟欣,韩利亚,等.刺五加苷E对小鼠学习及记忆能力的影响[J].药学实践杂志,2016,34(4):318-319,371.Wang Y,Zhai X,Han L Y,et al.Effects of eleutheroside Eon learning and memory ability in mice[J].Journal of Pharmaceutical Practice,2016,34(4):318-319,371.
19.陈晓辉.类风湿性关节炎小鼠模型的制备及刺五加皂苷E对其治疗作用的研究[D].苏州:苏州大学,2015.Chen X H.eleutheroside E ameliorates arthritis severity in collagen-induced arthritis mice model by suppressing inflammatory cytokine release[D].Suzhou:Soochow University,2015.
20.陈耿标,陈锦丽,刘映芬.异嗪皮啶对肝癌患者MMP-7表达水平和癌细胞侵袭能力的影响[J].白求恩医学杂志,2016,14(2):148-151.Chen G B,Chen J L,Liu Y F.Effect of Isofraxidinin on expression of MMP-7 levels and cancer cell invasion ability of patients with liver cancer[J].Journal of Bethune Medical Science,2016,14(2):148-151.
21.汪琢,姜守刚,郭晓帆,等.刺五加中异嗪皮啶提取工艺优化及其抗肿瘤、抗氧化活性研究[J].天然产物研究与开发,2015,27(6):1092-1098.Wang Z,Jiang S G,Guo X F,et al.Optimization of extraction conditions for Isofaxidin from Eleutherococcus senticosus and its antioxidant,antitumor activities[J].Natural Product Research and Development,2015,27(6):1092-1098.
22.Niu X F,Xing W,Li W F,et al.Isofraxidin exhibited antiinflammatory effects in vivo and inhibited TNF-αproduction in LPS-induced mouse peritoneal macrophages in vitro via the MAPK pathway[J].International Immunopharmacology,2012,14(2):164-171.
23.Yamazaki T,Shimosaka S,Sakurai M,et al.Anti-inflammatory effects of a major component of Acanthopanax senticosus Harms,isofraxidin[J].Seibutsu Butsuri Kagaku,2009,48(2):55-58.
24.孙建瑞,王大红,邱智军,等.连翘叶中连翘酯苷A、芦丁和连翘苷提取纯化工艺优化[J].食品科学,2017,38(14):200-205.Sun J R,Wang D H,Qiu Z J,et al.Simultaneous extraction purification of Forsythoside A,Rutin and Phillyrin from Forsythia suspensa leaves[J].Food Science,2017,38(14):200-205.
25.陈菊.中药槐米中芦丁提取工艺研究[J].中国医药科学,2017,7(15):51-53.Chen J.Study on the extraction technology of rutin in flos sophorae Immaturus[J].China Medicine and Pharmacy,2017,7(15):51-53.
26.赵强,赵海福.紫花苜蓿中芦丁的提取及抗菌活性研究[J].中国奶牛,2013(20):25-27.Zhao Q,Zhao H F.Study on extraction and antibacterial of rutin from Alfalfa[J].China Dairy Cattle,2013(20):25-27.
27.贾贵东,杨建雄,王莉,等.芦丁与绿原酸协同抗氧化活性研究[J].陕西师范大学学报:自然科学版,2010,38(5):61-64.Jia G D,Yang J X,Wang L,et al.Synergistic antioxidative activities of rutin and chlorogenic acid(CGA)[J].Journal of Shaanxi Normal University:Natural Science Edition,2010,38(5):61-64.
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2.白雪,胡文忠,姜爱丽,等.刺五加主要活性物质及其药理作用的研究进展[J].食品工业科技,2016,37(6):396-399.Bai X,Hu W Z,Jiang A L,et al.Research advance on main active substance and pharmacological action of Acanthopanax[J].Science and Technology of Food Industry,2016,37(6):396-399.
3.李心怡,陈玲,马逾英,等.不同栽培年限川桔梗中桔梗总皂苷和桔梗皂苷D的含量比较[J].中国药房,2018,29(9):1249-1252.Li X Y,Chen L,Ma Y Y,et al.Comparison of the content of total saponins and platycodin D in Platycodon grandiflorum from Sichuan of different cultivated years[J].China Pharmacy,2018,29(9):1249-1252.
4.殷少文,叶世芸,赵琳珺,等.干燥温度及生长年限对黔产青钱柳中多糖含量的影响[J].贵州农业科学,2018,46(1):82-85.Yin S W,Ye S Y,Zhao L J,et al.Effect of drying temperature and different growth years on polysaccharide content of Cyclocarya paliurus in Guizhou[J].Guizhou Agricultural Sciences,2018,46(1):82-85.
5.谭五丰,袁艳秋,于笛笛,等.刺五加与短梗五加中紫丁香苷、刺五加苷E及异嗪皮啶含量比较[J].现代食品科技,2017,33(10):302-307.Tan W F,Yuan Y Q,Yu D D,et al.Comparison of eleutheroside B,eleutheroside E,and Isofraxidin between Acanthopanax sentcosus(Ruper.et Maxim.)Harms and Acanthopanax sessiliflorus[J].Modern Food Science and Technology,2017,33(10):302-307.
6.张琳,刘洋,盖庆辉,等.HPLC-MS/MS法测定超声提取刺五加果实中刺五加苷B、E和异嗪皮啶的含量[J].上海中医药大学学报,2014,28(4):90-95.Zhang L,Liu Y,Gai Q H,et al.Determination of eleutheroside B,E and Isofraxidin in fruit of Radix et Caulis Acanthopanax senticosus by HPLC-MS/MS with ultrasonic-assisted extraction[J].Acta Universitatis Traditionis Medicalis Sinensis Pharmacologiaeque Shanghai,2014,28(4):90-95.
7.龚婧如,王书芳.HPLC多波长法测定刺五加颗粒中5个化合物的含量[J].药物分析杂志,2013,33(4):595-598,606.Gong J R,Wang S F.Determination of five components in Ciwujia granules by HPLC with multi-wavelengths[J].Chinese Journal of Pharmaceutical Analysis,2013,33(4):595-598,606.
8.范惠霞.刺五加中主要活性成分的药动学研究[D].济南:济南大学,2014.Fan H X.Pharmacokinetic study of the major active components of Acanthopanax senticoccus in rats[D].Jinan:Jinan University,2014.
9.吴高松,匡海学,王知斌,等.RP-HPLC法同时测定刺五加叶中芦丁、金丝桃苷和槲皮素的含量[J].化学工程师,2017,31(2):32-34.Wu G S,Kuang H X,Wang Z B,et al.Simultaneous determination of rutin,hyperin and quercetin in Acanthopanax senticocus leaves by RP-HPLC[J].Chemical Engineer,2017,31(2):32-34.
10.翟春梅,史连宏,陈忠新,等.UPLC-DAD法同时测定刺五加叶中Chlorogenic acid,Rutin,Hyperoside及Isoquercitrin四种成分的含量[J].中医药信息,2016,33(5):52-55.Zhai C M,Shi L H,Chen Z X,et al.UPLC-DAD method for quantification on Chlorogenic Acid,Rutin,Hyperoside and isoquercitrin in extract of E.senticosus leaf[J].Information on Traditional Chinese Medicine,2016,33(5):52-55.
11.张萍.HPLC测定不同产地、不同生长年限刺五加药材中异嗪皮啶[J].药物分析杂志,2010,30(12):2433-2435.Zhang P.HPLC determination of Isofraxidin in Acanthopanax senticosus(Rupr.et Maxim.)Harms of different sources and growth time limits[J].Chinese Journal of Pharmaceutical Analysis,2010,30(12):2433-2435.
12.王宗权,王振月,陈立超,等.刺五加不同采收年限、不同产地及不同加工方法的研究[J].中药研究与信息,2005,7(9):13-15.Wang Z Q,Wang Z,Chen L,et al.Studies on different harvest periods and different processing methods of Acanthopanax senticosus from different producing Areas[J].Research&Information of Traditional Chinese Medicine,2005,7(9):13-15.
13.白雪,胡文忠,姜爱丽,等.刺五加活性成分的研究进展[J].食品工业科技,2016,37(4):378-381.Bai X,Hu W Z,Jiang A L,et al.Research advance on Acanthopanax structure and the content of active ingredient[J].Science and Technology of Food Industry,2016,37(4):378-381.
14.秦湫红,朱爱华.紫丁香苷抗肿瘤活性筛选及作用机制研究[J].安徽农业科学,2018,46(14):107-108,112.Qin Q H,Zhu A H.Study on the screening and mechanism of syringin anticancer activity[J].Journal of Anhui Agricultural Sciences,2018,46(14):107-108,112.
15.徐颀.蒸制轮叶党参的化学成分分析及药理活性研究[D].长春:吉林农业大学,2016.Xu Q.Study on the chemical composition and biological activity of steamed Codonopsis lanceolata[D].Changchun:Jilin Agricultural University,2016.
16.Ahn J,Um M Y,Lee H,et al.eleutheroside E,an active component of Eleutherococcus senticosus,ameliorates insulin resistance in type 2 diabetic db/db mice[J].EvidenceBased Complementary and Alternative Medicine,2013,2013:934183.
17.Huang L Z,Wei L,Zhao H F,et al.The effect of eleutheroside E on behavioral alterations in murine sleep deprivation stress model[J].European Journal of Pharmacology,2011,658(2-3):150-155.
18.汪洋,翟欣,韩利亚,等.刺五加苷E对小鼠学习及记忆能力的影响[J].药学实践杂志,2016,34(4):318-319,371.Wang Y,Zhai X,Han L Y,et al.Effects of eleutheroside Eon learning and memory ability in mice[J].Journal of Pharmaceutical Practice,2016,34(4):318-319,371.
19.陈晓辉.类风湿性关节炎小鼠模型的制备及刺五加皂苷E对其治疗作用的研究[D].苏州:苏州大学,2015.Chen X H.eleutheroside E ameliorates arthritis severity in collagen-induced arthritis mice model by suppressing inflammatory cytokine release[D].Suzhou:Soochow University,2015.
20.陈耿标,陈锦丽,刘映芬.异嗪皮啶对肝癌患者MMP-7表达水平和癌细胞侵袭能力的影响[J].白求恩医学杂志,2016,14(2):148-151.Chen G B,Chen J L,Liu Y F.Effect of Isofraxidinin on expression of MMP-7 levels and cancer cell invasion ability of patients with liver cancer[J].Journal of Bethune Medical Science,2016,14(2):148-151.
21.汪琢,姜守刚,郭晓帆,等.刺五加中异嗪皮啶提取工艺优化及其抗肿瘤、抗氧化活性研究[J].天然产物研究与开发,2015,27(6):1092-1098.Wang Z,Jiang S G,Guo X F,et al.Optimization of extraction conditions for Isofaxidin from Eleutherococcus senticosus and its antioxidant,antitumor activities[J].Natural Product Research and Development,2015,27(6):1092-1098.
22.Niu X F,Xing W,Li W F,et al.Isofraxidin exhibited antiinflammatory effects in vivo and inhibited TNF-αproduction in LPS-induced mouse peritoneal macrophages in vitro via the MAPK pathway[J].International Immunopharmacology,2012,14(2):164-171.
23.Yamazaki T,Shimosaka S,Sakurai M,et al.Anti-inflammatory effects of a major component of Acanthopanax senticosus Harms,isofraxidin[J].Seibutsu Butsuri Kagaku,2009,48(2):55-58.
24.孙建瑞,王大红,邱智军,等.连翘叶中连翘酯苷A、芦丁和连翘苷提取纯化工艺优化[J].食品科学,2017,38(14):200-205.Sun J R,Wang D H,Qiu Z J,et al.Simultaneous extraction purification of Forsythoside A,Rutin and Phillyrin from Forsythia suspensa leaves[J].Food Science,2017,38(14):200-205.
25.陈菊.中药槐米中芦丁提取工艺研究[J].中国医药科学,2017,7(15):51-53.Chen J.Study on the extraction technology of rutin in flos sophorae Immaturus[J].China Medicine and Pharmacy,2017,7(15):51-53.
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