扁桃酸的镇痛抗炎作用研究
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摘要
目的:观察扁桃酸的镇痛、抗炎作用。方法:50只昆明种SPF级小鼠随机分为5组:空白对照组(生理盐水,0.1 ml/10 g)、扁桃酸高(300 mg·kg~(-1))、中(200 mg·kg~(-1))、低(140 mg·kg~(-1))剂量组、阿司匹林阳性对照组,ig,qd。采用小鼠扭体法和热板法观察扁桃酸的镇痛作用。采用二甲苯涂耳致小鼠急性耳肿胀观察其抗炎作用。结果:扁桃酸各剂量组均能使小鼠扭体次数明显减少,痛阈延长,与空白对照组比较差异有统计学意义(P<0.01)。扁桃酸高剂量组的小鼠扭体次数少于阳性对照组,差异无统计学意义(P>0.05),扁桃酸中、低剂量组的小鼠扭体次数多于阳性对照组,其中低剂量组差异有统计学意义(P<0.05);扁桃酸各剂量组用药前后小鼠的痛阈提高率均比阳性对照组高,其中高剂量组用药前后痛阈增加明显,与阳性对照组差异有统计学意义(P<0.05)。扁桃酸各剂量组对小鼠耳肿胀的影响无显著作用,与空白对照组比较差异无统计学意义(P>0.05)。结论:扁桃酸具有显著的镇痛作用,抗炎作用不明显。
Objective: To observe the analgesic and anti-inflammatory effect of mandelic acid. Methods: Fifty Kunming mice were randomly divided into 5 groups: the blank control group( 0. 1 ml/10 g),mandelic acid high( 300 mg·kg~(-1)),medium( 200 mg·kg~(-1)) and low( 140 mg·kg~(-1)) dose groups,and the positive control( aspirin) group,ig,qd. The analgesic effect of mandelic acid was observed by writhing test and hot plate method in mice. The ear swelling model caused by dimethyl benzene in mice was adopted to observe the analgesic effect. Results: Mandelic acid in each dose group could make the number of writhing in mice significantly reduced and pain threshold extended,and when compared with the blank control group,the difference was statistically significant( P < 0. 01). The writhing times of mice mandelic acid high dose group was fewer than that of the positive control group,and there was no statistically significant between the groups( P > 0. 05). In low and medium dose group,the writhing times of mice were more than those of the positive control group,and there was a significant difference between the low dose group and the positive control group( P< 0. 05). The pain threshold of the mice in each mandelic acid dose group was higher than that of the positive control group,the pain threshold increased significantly in the high dose group before and after the administration,and the difference was statistically significant when compared with the positive control group( P < 0. 05). The effect of mandelic acid on the ear swelling of mice was not significant,and when compared with the blank control group,the difference was not significant( P > 0. 05). Conclusion: Mandelic acid has significant analgesic effect,while anti-inflammatory effect is not obvious.
Objective: To observe the analgesic and anti-inflammatory effect of mandelic acid. Methods: Fifty Kunming mice were randomly divided into 5 groups: the blank control group( 0. 1 ml/10 g),mandelic acid high( 300 mg·kg~(-1)),medium( 200 mg·kg~(-1)) and low( 140 mg·kg~(-1)) dose groups,and the positive control( aspirin) group,ig,qd. The analgesic effect of mandelic acid was observed by writhing test and hot plate method in mice. The ear swelling model caused by dimethyl benzene in mice was adopted to observe the analgesic effect. Results: Mandelic acid in each dose group could make the number of writhing in mice significantly reduced and pain threshold extended,and when compared with the blank control group,the difference was statistically significant( P < 0. 01). The writhing times of mice mandelic acid high dose group was fewer than that of the positive control group,and there was no statistically significant between the groups( P > 0. 05). In low and medium dose group,the writhing times of mice were more than those of the positive control group,and there was a significant difference between the low dose group and the positive control group( P< 0. 05). The pain threshold of the mice in each mandelic acid dose group was higher than that of the positive control group,the pain threshold increased significantly in the high dose group before and after the administration,and the difference was statistically significant when compared with the positive control group( P < 0. 05). The effect of mandelic acid on the ear swelling of mice was not significant,and when compared with the blank control group,the difference was not significant( P > 0. 05). Conclusion: Mandelic acid has significant analgesic effect,while anti-inflammatory effect is not obvious.
引文
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11 卢敏.苦杏仁酸制备工艺研究[J].化工设计通讯,2016,42(7):308
12 夏敏杰,王玉柱,黄超,等.dl-扁桃酸对人精子与阴道黏膜上皮VK2/E6E7细胞的毒性研究[J].中国现代应用药学,2015,32(4):391-395
13 陈卓,冯锁民,杨建全.前药布洛芬扁桃酸酯的合成[J].应用化工,2013,42(10):1806-1807
14 刘真,马晓丽,夏甜甜,等.尼古丁对小鼠镇痛作用的研究[J].时珍国医国药,2013,24(2):279-280
2 王民,郭仁舆.扁桃酸的体外杀精子研究[J].西安医科大学学报,1988,9(4):33-36
3 张敏如,高玉珍,王云彩.扁桃酸栓治疗滴虫病的基础与临床研究[J].实用妇科与与产科杂志,1990,6(5):253-254
4 黄真,冯春荣.孝扇草水提液抗炎镇痛作用的实验研究[J].中华中医药学刊,2010,28(6):56-58
5 陈奇.中药药理研究方法学[M].北京:人民卫生出版社,1996:377
6 刘良.扁桃腈和扁桃酸的技术进展[J].精细化工化纤信息通讯,2002,(1):6
7 阮肖平,何昌国,林立,等.虎头蕉全草提取液抗炎、镇痛作用的实验研究[J].中国中医药科技,2010,17(3):219-221
8 黄蓓,毛长智,庾志斌.四数九里香水提物抗炎及镇痛作用研究[J].中国民族民间医药,2011,20(14):32-33
9 牛放,赵雨,杨菲,等.梅花鹿鹿角托盘提取物的抗炎镇痛作用[J].中国医院药学杂志,2011,31(10):789-791
10 毛理纳,罗予,洪素兰.乌头汤合并局部加温的镇痛抗炎作用[J].中国中西医结合杂志,1998,18(6):289-291
11 卢敏.苦杏仁酸制备工艺研究[J].化工设计通讯,2016,42(7):308
12 夏敏杰,王玉柱,黄超,等.dl-扁桃酸对人精子与阴道黏膜上皮VK2/E6E7细胞的毒性研究[J].中国现代应用药学,2015,32(4):391-395
13 陈卓,冯锁民,杨建全.前药布洛芬扁桃酸酯的合成[J].应用化工,2013,42(10):1806-1807
14 刘真,马晓丽,夏甜甜,等.尼古丁对小鼠镇痛作用的研究[J].时珍国医国药,2013,24(2):279-280