气质联用结合保留指数分析海南九里香叶和果实中的挥发油成分
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摘要
目的研究比较海南产九里香叶和果实中挥发油的化学成分及相对含量。方法采用水蒸气蒸馏法提取九里香叶和果实中的挥发油,气相色谱-质谱联用技术(GC-MS)结合保留指数进行分析和鉴定。结果从九里香叶和果实的挥发油中分别鉴定出挥发性化合物61种和44种,分别占挥发油总量的96.2%和99.4%。结论九里香叶和果实的挥发油存在较大差异,其主要成分倍半萜的含量分别为91.9%和43.4%,其中共有成分23种。该方法为九里香进一步的研究及开发利用提供了参考。
Objective To determine the contents of essential oils from leaves and fruits of Murraya exotica. Methods The essential oils were extracted from the fruits and leaves of Murraya exotica by hydrodistillation,and analyzed and identified by gas chromatography-mass spectrometry( GC-MS) combined with Kovat's retention index( KI). Results Sixty-one chemical components were identified from the essential oils of leaves,and forty-four from the essential oil of fruits,representing the relative contents of 96. 2% and 99. 4% of the total oil. Conclusion The chemical components in the oils from leaves and fruits exhibited significant differences and the major components were sesquiterpenes,representing 91. 9% from leaves and 43. 4% from fruits. Twenty three common components were identified in both the leaves and fruits. The results may provide experimental basis for further exploitation of Murraya exotica.
Objective To determine the contents of essential oils from leaves and fruits of Murraya exotica. Methods The essential oils were extracted from the fruits and leaves of Murraya exotica by hydrodistillation,and analyzed and identified by gas chromatography-mass spectrometry( GC-MS) combined with Kovat's retention index( KI). Results Sixty-one chemical components were identified from the essential oils of leaves,and forty-four from the essential oil of fruits,representing the relative contents of 96. 2% and 99. 4% of the total oil. Conclusion The chemical components in the oils from leaves and fruits exhibited significant differences and the major components were sesquiterpenes,representing 91. 9% from leaves and 43. 4% from fruits. Twenty three common components were identified in both the leaves and fruits. The results may provide experimental basis for further exploitation of Murraya exotica.
引文
[1]国家药典委员会.中国药典:第四部[M].北京:中国医药科技出版社,2015:6.
[2]骆焱平,卢远倩,夏治琴,等.九里香层析物的抑菌活性及成分分析[J].西北农业学报,2011,20(9):180-183.
[3]Sundaram M,Sivakumar,Karthikeyan,et al.Studies on in vitro antibacterial,antifungal property and antioxidant potency of Murraya paniculata[J].Pak J Nutr,2011,10(10):925-929.
[4]陈琼华,王淑如,张宗禹,等.九里香的抗生育作用[J].中国药科大学学报,1987,18(3):213-215.
[5]Mehmood F,Manzoor F,Khan ZUD,et al.Evaluation of toxicity and repellency of essential oils of family rutaceae against black ants(Lasius niger)in Pakistan[J].Asian J Chem,2012,24(7):3087-3090.
[6]毛长智,黄蓓,庾志斌.四数九里香挥发油抗炎及镇痛作用研究[J].云南中医中药杂志,2011,32(8):74-75.
[7]卢远倩,王兰英,骆焱平.九里香精油的抑菌活性及成分分析[J].农药,2011,50(6):443-445,448.
[8]Chowdhury JU,Bhuiyan MNI,Yusuf M.Chemical composition of the leaf essential oils of Murraya koenigii(L.)Spreng and Murraya paniculata(L.)Jack[J].Bangladesh J Pharmacol,2008;3(2):59-63.
[9]姜平川,周军,曹斌,等.九里香挥发油成分研究[J].中药材,2009,32(8):1224-1227.
[10]龚志强,谈远锋,黄敏,等.不同方法提取四数九里香挥发油气质联用分析[J].湖南师范大学自然科学学报,2014,37(2):47-50.
[11]国家药典委员会.中国药典:第四部[M].北京:中国医药科技出版社,2015:6.
[12]梁晟,李雅文,赵晨曦,等.GC-MS结合保留指数对中药挥发油的定性[J].分析测试学报,2008,27(1):84-87.
[2]骆焱平,卢远倩,夏治琴,等.九里香层析物的抑菌活性及成分分析[J].西北农业学报,2011,20(9):180-183.
[3]Sundaram M,Sivakumar,Karthikeyan,et al.Studies on in vitro antibacterial,antifungal property and antioxidant potency of Murraya paniculata[J].Pak J Nutr,2011,10(10):925-929.
[4]陈琼华,王淑如,张宗禹,等.九里香的抗生育作用[J].中国药科大学学报,1987,18(3):213-215.
[5]Mehmood F,Manzoor F,Khan ZUD,et al.Evaluation of toxicity and repellency of essential oils of family rutaceae against black ants(Lasius niger)in Pakistan[J].Asian J Chem,2012,24(7):3087-3090.
[6]毛长智,黄蓓,庾志斌.四数九里香挥发油抗炎及镇痛作用研究[J].云南中医中药杂志,2011,32(8):74-75.
[7]卢远倩,王兰英,骆焱平.九里香精油的抑菌活性及成分分析[J].农药,2011,50(6):443-445,448.
[8]Chowdhury JU,Bhuiyan MNI,Yusuf M.Chemical composition of the leaf essential oils of Murraya koenigii(L.)Spreng and Murraya paniculata(L.)Jack[J].Bangladesh J Pharmacol,2008;3(2):59-63.
[9]姜平川,周军,曹斌,等.九里香挥发油成分研究[J].中药材,2009,32(8):1224-1227.
[10]龚志强,谈远锋,黄敏,等.不同方法提取四数九里香挥发油气质联用分析[J].湖南师范大学自然科学学报,2014,37(2):47-50.
[11]国家药典委员会.中国药典:第四部[M].北京:中国医药科技出版社,2015:6.
[12]梁晟,李雅文,赵晨曦,等.GC-MS结合保留指数对中药挥发油的定性[J].分析测试学报,2008,27(1):84-87.