磁性氧化石墨烯固相萃取酶联免疫吸附法测定芝麻油中黄曲霉毒素B_1含量
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摘要
通过共沉淀法合成磁性氧化石墨烯作为磁固相萃取材料,结合酶联免疫吸附法(ELISA)建立测定芝麻油中黄曲霉毒素B_1含量的新方法。试验考察了萃取条件和洗脱条件等参数对黄曲霉毒素B_1吸附效率的影响,在最优的试验条件下,检出灵敏度为0.05μg/kg,日内和日间相对标准偏差分别为3.75%～5.14%和5.26%～7.15%,实际样品加标回收率在80.6%～92.3%。结果表明,该方法稳定性好、测定准确,适用于芝麻油中黄曲霉毒素B_1含量的检测。
Magnetic graphene oxide was synthesized by coprecipitation method, which was used as magnetic adsorbent a new way of determination of aflatoxin B_1 in sesame oil by magnetic solid phase extraction linked with ELISA. The major parameters affecting extraction efficiency were studied, including extraction conditions and elution conditions. Under the optimized experimental conditions, the limit of detection(LOD)was calculated as 0.05 μg/kg, the relative standard diviations of intra-day and inter-day repeated experiments was 3.75 %-5.14 % and 5.26 %-7.15 %, respectively. The recoveries of aflatoxin B_1 in sesame oil samples were in the range of 80.6 %-92.3 %, the results showed the method had good stability and accuracy, and which was suitable for the determination of aflatoxin B_1 in sesame oil.
Magnetic graphene oxide was synthesized by coprecipitation method, which was used as magnetic adsorbent a new way of determination of aflatoxin B_1 in sesame oil by magnetic solid phase extraction linked with ELISA. The major parameters affecting extraction efficiency were studied, including extraction conditions and elution conditions. Under the optimized experimental conditions, the limit of detection(LOD)was calculated as 0.05 μg/kg, the relative standard diviations of intra-day and inter-day repeated experiments was 3.75 %-5.14 % and 5.26 %-7.15 %, respectively. The recoveries of aflatoxin B_1 in sesame oil samples were in the range of 80.6 %-92.3 %, the results showed the method had good stability and accuracy, and which was suitable for the determination of aflatoxin B_1 in sesame oil.
引文
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[5]MANAFI M H, ALLAHYARI M, POURGHAZI K, et al. Surfactantenhanced spectrofluorimetric determination of total aflatoxins from wheat samples after magnetic solid-phase extraction using modi?ed Fe3O4 nanoparticles[J]. Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy, 2015, 146:43-49.
[6]吴科盛,许恒毅,郭亮,等.磁性固相萃取在检测分析中的应用研究进展[J].食品科学, 2011, 32(23):317-320.
[7]WU R, MA F, ZHANG L, et al. Simultaneous determination of phenolic compounds in sesame oil using LC-MS/MS combined with magnetic carboxylated multi-walled carbon nanotubes[J]. Food Chemistry, 2016, 204:334-342.
[8]RAN C, CHEN D, MA H, et al. Graphene oxide adsorbent based dispersive solid phase extraction coupled with multi-pretreatment clean-up for analysis of trace aflatoxins in traditional proprietary Chinese medicines[J]. Journal of Chromatography B, 2017, 1044-1045:120-126.
[9]刘泽玲.磁性氧化石墨烯复合材料的制备及其吸附染料的性能研究[D].天津:天津工业大学, 2017.
[2]PI F, SUN J, LIU W, et al. Elimination of aflatoxin B1 in vegetable oil based onimmuno-magnetosomes probes from a novel magnetotactic bacterium[J]. Food Control, 2017, 80:319-326.
[3]YU L, LI P, ZHANG Q, et al. Graphene oxide:An adsorbent for the extraction and quanti?cation of aflatoxins in peanuts by highperformance liquid chromatography[J]. Journal of Chromatography A, 2013, 1318:27-34.
[4]李江,綦艳,佘之蕴,等.酶联免疫法对食用油中黄曲霉毒素B1的检测[J].广东化工, 2017, 44(11):73-74.
[5]MANAFI M H, ALLAHYARI M, POURGHAZI K, et al. Surfactantenhanced spectrofluorimetric determination of total aflatoxins from wheat samples after magnetic solid-phase extraction using modi?ed Fe3O4 nanoparticles[J]. Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy, 2015, 146:43-49.
[6]吴科盛,许恒毅,郭亮,等.磁性固相萃取在检测分析中的应用研究进展[J].食品科学, 2011, 32(23):317-320.
[7]WU R, MA F, ZHANG L, et al. Simultaneous determination of phenolic compounds in sesame oil using LC-MS/MS combined with magnetic carboxylated multi-walled carbon nanotubes[J]. Food Chemistry, 2016, 204:334-342.
[8]RAN C, CHEN D, MA H, et al. Graphene oxide adsorbent based dispersive solid phase extraction coupled with multi-pretreatment clean-up for analysis of trace aflatoxins in traditional proprietary Chinese medicines[J]. Journal of Chromatography B, 2017, 1044-1045:120-126.
[9]刘泽玲.磁性氧化石墨烯复合材料的制备及其吸附染料的性能研究[D].天津:天津工业大学, 2017.