摘要
为拓展木霉T6与青霉K两种生防菌的使用范围,将二者混合培养后测定其溶磷能力及其培养液的促生作用。解磷试验结果表明:木霉T6和青霉K混合培养溶磷量呈现先增高后逐渐稳定的趋势,混合培养溶磷量较木霉T6和青霉K单独培养时的解磷量分别增加了646.52%和17.82%。第7 d的溶磷量达到最大,为614.06μg/mL。且两种菌株混合培养后溶磷量的变化趋势与混合发酵液pH值的变化趋势相反;盆栽试验结果表明,木霉T6与青霉K的混合培养液对小麦和白菜幼苗的生长具有明显的促生作用,能够显著增加幼苗根长、株高、鲜干重及叶绿素含量,且促生效果高于木霉T6和青霉K单独处理后的效果。由此得出,木霉T6与青霉K菌株混合培养具有很好的溶磷促生作用。
In order to expand the range of use of two biocontrol fungi,Trichoderma T6 and Penicillium K,the two were mixed and cultured to determine the level of their phosphorus solubilization and the growth-promoting effect of the cultured solution.The results showed that the amount of phosphorus dissolved in the mixed culture of Trichoderma T6 and Penicillium K increased firstly and then gradually stabilized.The amount of dissolved phosphorus in the mixed culture increased to 646.52% and 17.82% compared with that of Trichoderma T6 and Penicillium K.The amount of phosphorus dissolved on the 7 th day reached a maximum of 61.06 μg/mL and the change trend of the amount of dissolved phosphorus was opposite to the change of pH value of each fermentation broth.The results of pot experiment showed that the mixed culture solution of Trichoderma T6 and Penicillium K had obvious promoting effect on the growth of wheat and Chinese cabbage seedlings.The seedling root length,plant height,fresh dry weight and chlorophyll content were signifcantly increased,and the growth promoting effect was signifcantly higher than that of Trichoderma T6 and Penicillium K alone.It is concluded that the mixed culture of Trichoderma T6 and Penicillium K strain had a good role in promoting phosphorus solubilization,which provides an important theoretical basis for the development of the two mixed biocontrol agents and their applications.
引文
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