木霉T6和青霉K菌株混合培养的溶磷促生效应
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摘要
为拓展木霉T6与青霉K两种生防菌的使用范围,将二者混合培养后测定其溶磷能力及其培养液的促生作用。解磷试验结果表明:木霉T6和青霉K混合培养溶磷量呈现先增高后逐渐稳定的趋势,混合培养溶磷量较木霉T6和青霉K单独培养时的解磷量分别增加了646.52%和17.82%。第7 d的溶磷量达到最大,为614.06μg/mL。且两种菌株混合培养后溶磷量的变化趋势与混合发酵液pH值的变化趋势相反;盆栽试验结果表明,木霉T6与青霉K的混合培养液对小麦和白菜幼苗的生长具有明显的促生作用,能够显著增加幼苗根长、株高、鲜干重及叶绿素含量,且促生效果高于木霉T6和青霉K单独处理后的效果。由此得出,木霉T6与青霉K菌株混合培养具有很好的溶磷促生作用。
In order to expand the range of use of two biocontrol fungi,Trichoderma T6 and Penicillium K,the two were mixed and cultured to determine the level of their phosphorus solubilization and the growth-promoting effect of the cultured solution.The results showed that the amount of phosphorus dissolved in the mixed culture of Trichoderma T6 and Penicillium K increased firstly and then gradually stabilized.The amount of dissolved phosphorus in the mixed culture increased to 646.52% and 17.82% compared with that of Trichoderma T6 and Penicillium K.The amount of phosphorus dissolved on the 7 th day reached a maximum of 61.06 μg/mL and the change trend of the amount of dissolved phosphorus was opposite to the change of pH value of each fermentation broth.The results of pot experiment showed that the mixed culture solution of Trichoderma T6 and Penicillium K had obvious promoting effect on the growth of wheat and Chinese cabbage seedlings.The seedling root length,plant height,fresh dry weight and chlorophyll content were signifcantly increased,and the growth promoting effect was signifcantly higher than that of Trichoderma T6 and Penicillium K alone.It is concluded that the mixed culture of Trichoderma T6 and Penicillium K strain had a good role in promoting phosphorus solubilization,which provides an important theoretical basis for the development of the two mixed biocontrol agents and their applications.
In order to expand the range of use of two biocontrol fungi,Trichoderma T6 and Penicillium K,the two were mixed and cultured to determine the level of their phosphorus solubilization and the growth-promoting effect of the cultured solution.The results showed that the amount of phosphorus dissolved in the mixed culture of Trichoderma T6 and Penicillium K increased firstly and then gradually stabilized.The amount of dissolved phosphorus in the mixed culture increased to 646.52% and 17.82% compared with that of Trichoderma T6 and Penicillium K.The amount of phosphorus dissolved on the 7 th day reached a maximum of 61.06 μg/mL and the change trend of the amount of dissolved phosphorus was opposite to the change of pH value of each fermentation broth.The results of pot experiment showed that the mixed culture solution of Trichoderma T6 and Penicillium K had obvious promoting effect on the growth of wheat and Chinese cabbage seedlings.The seedling root length,plant height,fresh dry weight and chlorophyll content were signifcantly increased,and the growth promoting effect was signifcantly higher than that of Trichoderma T6 and Penicillium K alone.It is concluded that the mixed culture of Trichoderma T6 and Penicillium K strain had a good role in promoting phosphorus solubilization,which provides an important theoretical basis for the development of the two mixed biocontrol agents and their applications.
引文
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[13] 孙磊,程显好,李维焕,等.菌落直径法和菌丝干重法在优化真姬菇菌种固体斜面培养条件的比较[J].中国食用菌,2015,34(3):28-32.
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[16] 刘青海.六株溶磷菌与四株固氮菌互作效应及其菌剂对苜蓿促生效果研究[D].兰州:甘肃农业大学,2011.
[17] 管国强,李倩,尚磊,等.菌株混合培养的溶磷解钾效果及条件优化[J].湖北农业科学,2016,55(2):323-327.
[18] 苌豹.多种解磷、解钾、固氮菌混合发酵制备菌糠菌肥的研究[D].天津:天津大学,2014.
[19] 林英,司春灿,韩文华,等.解磷微生物研究进展[J].江西农业学报,2017,29(2):99-103.
[20] N B Paul,W V B SundaraRao.Phosphate-dissolving bacteria in the rhizosphere of some cultivated legumes[J].Plant and Soil,1971,35(1):53-58.
[21] 张萌.海洋细菌AiL3和CⅢ-1菌株混合发酵及对辣椒促生作用研究[D].湛江:广东海洋大学,2013.
[22] 赵雅峰,李春,沈亁炜,等.产酸克雷伯氏菌Rs-5与枯草芽孢杆菌组合BCL-8复配对棉花的解盐促生作用[J].安徽农业科学,2010,38(12):6103-6105.
[2] 张曼曼,邓春生,马金奉,等.多功能木霉的筛选及鉴定[J].农业环境科学学报,2012,31(8):1571-1575.
[3] 杨春林,席亚东,刘波微,等.哈茨木霉T-h-30对几种蔬菜的促生作用及病害防治初探[J].西南农业学报,2008,21(6):1603-1607.
[4] 陈臻,古丽君,徐秉良,等.长枝木霉对6种牧草种子发芽与生理效应的影响[J].草地学报,2013,21(3):564-570.
[5] 高学彪,邓穗儿,周慧娟,等.淡紫拟青霉MCWA18菌株对南方根结线虫的寄生和防治作用[J].中国生物防治,1998,15(4):20-23.
[6] JatalaP.Biologicalcontrol of plant-parasitic nematodes[J].Annual Review of Phytopathology,1986,24:453-489.
[7] JatalaP,Kaltenbach R.Biological control of Meloidogyne incognita and Globoderapallida on potatoes[J].Journal of Nematology,1979,11:303.
[8] 李勤生,卫翔.混合培养对光合细菌生长量的影响[J].水生生物学报,1998,22(2):101-105.
[9] 韩梅,李丽娜,魏冉,等.混合培养提高菌株解磷解钾能力的探讨[J].微生物学杂志,2010,30(5):74-77.
[10] 韩梅,魏冉,李丽娜,等.混合培养对菌剂固氮、解磷和解钾能力的影响[J].沈阳农业大学学报,2010,41(5):622-625.
[11] 薛应钰,叶巍,张树武,等.紫外诱变选育木霉高效解磷菌株[J].核农学报,2015,29(8):1509-1516.
[12] 任友花,王羿超,李娜,等.微生物肥料高效解磷菌筛选及解磷机理探究[J].江苏农业科学,2016,44(12):537-540.
[13] 孙磊,程显好,李维焕,等.菌落直径法和菌丝干重法在优化真姬菇菌种固体斜面培养条件的比较[J].中国食用菌,2015,34(3):28-32.
[14] Vassilev N,Medina A,Azcon R,et al.Microbial solubilization of rock phosphate on media containing agro-industrial wastes and effect of the resulting products on plant growth and P uptake[J].Plant and Soil,2006,287(1):77-84
[15] 刘莉华,邹东福.植物叶片中叶绿素含量测定方法的优化[J].中国科技博览,2013(3):463-463.
[16] 刘青海.六株溶磷菌与四株固氮菌互作效应及其菌剂对苜蓿促生效果研究[D].兰州:甘肃农业大学,2011.
[17] 管国强,李倩,尚磊,等.菌株混合培养的溶磷解钾效果及条件优化[J].湖北农业科学,2016,55(2):323-327.
[18] 苌豹.多种解磷、解钾、固氮菌混合发酵制备菌糠菌肥的研究[D].天津:天津大学,2014.
[19] 林英,司春灿,韩文华,等.解磷微生物研究进展[J].江西农业学报,2017,29(2):99-103.
[20] N B Paul,W V B SundaraRao.Phosphate-dissolving bacteria in the rhizosphere of some cultivated legumes[J].Plant and Soil,1971,35(1):53-58.
[21] 张萌.海洋细菌AiL3和CⅢ-1菌株混合发酵及对辣椒促生作用研究[D].湛江:广东海洋大学,2013.
[22] 赵雅峰,李春,沈亁炜,等.产酸克雷伯氏菌Rs-5与枯草芽孢杆菌组合BCL-8复配对棉花的解盐促生作用[J].安徽农业科学,2010,38(12):6103-6105.