氯化钾溶液中羟苯乙酯含量考察
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摘要
目的:研究氯化钾溶液处方中抑菌剂羟苯乙酯含量。方法:参照中国药典2015年版四部抑菌效力检查法,以药典规定的5种标准菌株(金黄色葡萄球菌、大肠埃希菌、铜绿假单胞菌、白色念珠菌和黑曲霉)作为挑战菌,以菌落的对数下降值为指标,分别对不同浓度羟苯乙酯的抑菌效果进行考察,筛选出适宜的羟苯乙酯加入量。结果:氯化钾溶液中羟苯乙酯的加入量为0.05%,对5种挑战菌均有良好的抑制作用,且为最低有效剂量。结论:可选择0.05%羟苯乙酯作为氯化钾溶液的抑菌剂。
Objective: To investigate the content of ethylparaben in potassium chloride solution. Methods: According to the guidance for antibacterial effect test stated in Chinese Pharmacopoeia( the 4~(th) volume of 2015 edition),5 standard bacterial strains including Staphylococcus aureus,Escherichia coli,Pseudomonas aeruginosa,Candida albicans and Aspergillus niger were used as the challenging strains. Using the logarithm decrease of bacteria number as the index,the antimicrobial effectiveness of ethylparaben at different concentrations was studied to screen out the optimal concentration in the solution. Results: The growth of the 5 standard bacterial strains was inhibited effectively by potassium chloride solution containing 0. 05% ethylparaben,which was also the minimum effective concentration. Conclusion: 0. 05% Ethylparaben is suitable as the bacteriostatic agent for potassium chloride solution.
Objective: To investigate the content of ethylparaben in potassium chloride solution. Methods: According to the guidance for antibacterial effect test stated in Chinese Pharmacopoeia( the 4~(th) volume of 2015 edition),5 standard bacterial strains including Staphylococcus aureus,Escherichia coli,Pseudomonas aeruginosa,Candida albicans and Aspergillus niger were used as the challenging strains. Using the logarithm decrease of bacteria number as the index,the antimicrobial effectiveness of ethylparaben at different concentrations was studied to screen out the optimal concentration in the solution. Results: The growth of the 5 standard bacterial strains was inhibited effectively by potassium chloride solution containing 0. 05% ethylparaben,which was also the minimum effective concentration. Conclusion: 0. 05% Ethylparaben is suitable as the bacteriostatic agent for potassium chloride solution.
引文
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15 王虎斌,韦超,赵日秋,等.拉坦前列素滴眼液的抑菌效力研究[J].中国药房,2013,24(1):42-44
2 中国药典[S].2015年版.四部.通则.140-149
3 中国食品药品检定研究院.中国药品检验标准操作规范[M].北京:中国医药科技出版社,2010:351-374
4 夏晓君,董爱梅,张建强.0.1%玻璃酸钠滴眼液的抑菌效力研究[J].药学研究,2015,34(8):455-457
5 苏德模,马绪荣,许华玉,等.药品微生物检验技术[M].北京:华龄出版社,2007:473
6 杨静,李晓翠,徐轶飞,等.四种医院制剂微生物限度检查法验证试验[J].中国医药科学,2015,5(19):51-53
7 柳艳,王志宏,曲金瑶,等.乙酰半胱氨酸口服溶液抑菌剂抑菌效力研究[J].药学研究,2015,34(3):152-155
8 温东妹,夏春森,冯柏康.利巴韦林口服溶液中抑菌剂的筛选[J].中国药房,2010,21(45):4251-1254
9 江志杰,李玉立,刘文杰,等.吡诺克辛滴眼液的抑菌效力评价[J].中国现代应用药学,2015,32(6):704-708
10 肖璜,林吉恒,陈万胜.氯霉素滴眼液中尼泊金乙酯含量合理性的探讨[J].中国药师,2014,17(5):785-788
11 杨薇.盐酸莫西沙星滴眼液的抑菌效力研究[J].中国医药指南,2015,13(19):61-62
12 郭新营,臧恒昌,王彦厚.管碟法比较不同厂家的注射用头孢曲松钠的抑菌效力[J].药学研究,2015,34(12):711-713
13 高敏,杨明芳,黄婷.色甘酸钠滴眼液中对羟基苯甲酸乙酯最低有效抑菌浓度考察[J].药物分析杂志,2014,34(8):1402-1406
14 董自艳,戴翚.氯霉素滴眼液的抑菌效力评价[J].中国现代药物应用,2015,9(13):273-274
15 王虎斌,韦超,赵日秋,等.拉坦前列素滴眼液的抑菌效力研究[J].中国药房,2013,24(1):42-44