饲用微生物添加剂地衣芽孢杆菌活菌计数不确定度评定方法的建立
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摘要
目的探讨检测实验室饲用微生物添加剂地衣芽孢杆菌活菌计数的不确定度评价方法。方法实验依据JJF1059.1-2012《测量不确定度评定与表示》、RB/T151-2016《食品微生物定量检测的测量不确定度评估指南》和NY/T 1461-2007《饲料微生物添加剂地衣芽孢杆菌》,对地衣芽孢杆菌活菌计数的不确定度分量进行分析。结果本研究建立了地衣芽孢杆菌活菌计数不确定度评定方法,当包含因子k=2时,扩展不确定度U=4.05%,样品的误差允许范围在0.95×1010～1.15×1010 CFU/g之间。结论建立的地衣芽孢杆菌活菌计数不确定度评定方法简单、易行,符合实际检测要求。
Objective To explore the method of uncertainty evaluation for viable count of Bacillus Licheniformis as feed microorganism additive. Methods The uncertainty components were analyzed according to JJF 1059.1-2012 Evaluation and representation of measurement uncertainty, RB/T 151-2016 Guidelines for the estimation of measurement uncertainty of food microbiological quantitative detection and NY/T 1461-2007 Bacillus licheniformis as feed microbial additives. Results This method could evaluate the uncertainty of viable count of Bacillus licheniformis. When the factor k=2, the expanded uncertainty U was 4.05%. The allowable error range of the sample were between 0.95×10~(10)-1.15×10~(10) CFU/g. Conclusion This method for evaluating the uncertainty of viable count of Bacillus licheniformis is simple and feasible, which meets the actual detection requirements.
Objective To explore the method of uncertainty evaluation for viable count of Bacillus Licheniformis as feed microorganism additive. Methods The uncertainty components were analyzed according to JJF 1059.1-2012 Evaluation and representation of measurement uncertainty, RB/T 151-2016 Guidelines for the estimation of measurement uncertainty of food microbiological quantitative detection and NY/T 1461-2007 Bacillus licheniformis as feed microbial additives. Results This method could evaluate the uncertainty of viable count of Bacillus licheniformis. When the factor k=2, the expanded uncertainty U was 4.05%. The allowable error range of the sample were between 0.95×10~(10)-1.15×10~(10) CFU/g. Conclusion This method for evaluating the uncertainty of viable count of Bacillus licheniformis is simple and feasible, which meets the actual detection requirements.
引文
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[13]孟繁磊,牛红红,蔡红梅,等.分光光度法测定水中亚硝酸盐含量的不确定度评定[J].食品研究与开发,2016,37(9):174-177.Meng FL,Niu HH,Cai HM,et al.Evaluation of uncertainty in spectrophotometric determination of nitrite content in water[J].Food Res Dev,2016,37(9):174-177.
[14]张莹,远辉,丁春瑞.超高效液相色谱法测定白酒中纽甜不确定度评定[J].酿酒科技,2016,(12):99-101.Zhang Y,Yuan H,Ding CR.Evaluation of uncertainty in the determination of Niutan in liquor by ultra high performance liquid chromatography[J].Liquor-Mak Sci Technol,2016,(12):99-101.
[15]王忠一,吴帅.高效液相色谱法测定碳酸饮料中阿力甜含量的不确定度评定[J].中国食品添加剂,2016,(4):189-194.Wang ZY,Wu S.Evaluation of uncertainty in the determination of Alisma in carbonated beverages by high performance liquid chromatography[J].China Food Addit,2016,(4):189-194.
[2]雷质文.食品微生物实验室质量管理手册[M].北京:中国标准出版社,2006.Lei ZW.Handbook of quality management of food microbiology laboratory[M].Beijing:China Standards Publishing House,2006.
[3]秦文,王丽媛,王晶波,等.酸奶中乳酸菌计数不确定度的评定[J].中国卫生检验杂志,2018,28(15):1844-1846.Qin W,Wang LY,Wang JB,et al.Uncertainty evaluation of lactic acid bacteria count in yoghurt[J].Chin J Health Lab,2018,28(15):1844-1846.
[4]车方萍,赵梦芸,陈艳.食品(糕点)中菌落总数测量不确定度评定[J].现代食品,2018,(14):100-102.Che FP,Zhao MY,Chen Y.Evaluation of uncertainty in measurement of total number of colonies in foods(pastry)[J].Mod Food,2018,(14):100-102.
[5]杨玲玲,李海芳.食品菌落总数测定盲样考核结果不确定度的评定[J].食品安全质量检测学报,2018,9(14):3780-3783.Yang LL,Li HF.Uncertainty evaluation of determination of aerobic plate count in food by blindness examination[J].J Food Saf Qual,2018,9(14):3780-3783.
[6]岳苑,马桂娟.全脂乳粉中菌落总数的测量不确定度评定[J].食品与发酵科技,2018,54(3):124-126.Yue Y,Ma GJ.Uncertainty measurement of total colony in whole milk powder products[J].Food Ferment Technol,2018,54(3):124-126.
[7]李玉玲,宋莉莉.食品微生物学菌落总数检验中不确定度的评定[J].中国卫生检验杂志,2005,15(7):880-880.Li YL,Song LL.Evaluation of uncertainty in the total number of colonies in food microbiology[J].Chin J Health Lab Technol,2005,15(7):880-880.
[8]张虹仙.能力验证菌落总数测定结果不确定度评定[J].中国食品,2018,(11):145-147.Zhang HX.Evaluation of uncertainty in the measurement of total colony number by capability verification[J].China Food,2018,(11):145-147.
[9]冯秀娟,邓军,高俊峰.发酵乳中乳酸菌计数不确定度的评定[J].现代食品,2018,(1):93-97.Feng XJ,Deng J,Gao JF.Evaluation of uncertainty in number of lactic acid bacteria in fermented milk[J].Mod Food,2018,(1):93-97.
[10]王娟,许春,李兵兰,等.荆防颗粒微生物需氧菌总数不确定度评定[J].中国药师,2018,21(2):363-365.Wang J,Xu C,Li BL,et al.Evaluation of uncertainty of total aerobic bacteria in Jingfang granules[J].China Pharm,2018,21(2):363-365.
[11]刘培海,王玉兰,郑家利,等.进口瓶装饮用水中菌落总数不确定度的评定[J].中国卫生检验杂志,2014,(7):89-91.Li PH,Wang YL,Zheng JL,et al.Evaluation of uncertainty of total colony number in imported bottled drinking water[J].Chin J Health Lab Technol,2014,(7):89-91.
[12]张勇,王闻卿,张勇琪,等.评定食品中菌落总数的不确定度[J].临床医药文献电子杂志,2015,(5):811-812.Zhang Y,Wang WQ,Zhang YQ,et al.Uncertainty in assessing the number of colonies in food[J].J Clin Med Literat(Electr Ed),2015,(5):811-812.
[13]孟繁磊,牛红红,蔡红梅,等.分光光度法测定水中亚硝酸盐含量的不确定度评定[J].食品研究与开发,2016,37(9):174-177.Meng FL,Niu HH,Cai HM,et al.Evaluation of uncertainty in spectrophotometric determination of nitrite content in water[J].Food Res Dev,2016,37(9):174-177.
[14]张莹,远辉,丁春瑞.超高效液相色谱法测定白酒中纽甜不确定度评定[J].酿酒科技,2016,(12):99-101.Zhang Y,Yuan H,Ding CR.Evaluation of uncertainty in the determination of Niutan in liquor by ultra high performance liquid chromatography[J].Liquor-Mak Sci Technol,2016,(12):99-101.
[15]王忠一,吴帅.高效液相色谱法测定碳酸饮料中阿力甜含量的不确定度评定[J].中国食品添加剂,2016,(4):189-194.Wang ZY,Wu S.Evaluation of uncertainty in the determination of Alisma in carbonated beverages by high performance liquid chromatography[J].China Food Addit,2016,(4):189-194.