苦瓜中抑菌物质的提取及其生物活性初步分析
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摘要
选择合适的提取剂从苦瓜中提取具有抑菌效果的物质并进行生物活性分析.有效提取方法是使用浓度为70%的乙醇浸提,超声波破碎,正丁醇萃取,萃取物再通过聚酰胺分离纯化后,使用不同浓度乙醇进行洗脱,粗提液、萃取相和洗脱液的抑菌实验结果表明,苦瓜提取物对大肠杆菌、金黄色葡萄球菌、铜绿假单胞菌均具有明显的抑菌活性;抗氧化实验结果表明苦瓜提取物对H_2O_2均有明显的清除作用;定性分析显示提取物中含有皂苷、黄酮、多酚和有机酸等物质.
A suitable extraction method was selected to extract the antibacterial substance from bitter melon and the bioactivity was analyzed in this text.The effective method is extracting with 70% ethanol,breaking with ultrasonic,extracting with n-butyl alcohol,and then using different concentrations of ethanol for elution from polyamide column.The results of the experiments indicated that the crude extract,the n-butyl alcohol extraction phase and the elution showed obviously antibacterial activity to Escherichia coli,Staphylococcus aureus and Pseudomonas aeruginosa. The extracts in this text of bitter melon obviously cleared the H_2O_2. Qualitative analysis showed that the extracts contained saponins,flavonoids,polyphenols and organic acids.
A suitable extraction method was selected to extract the antibacterial substance from bitter melon and the bioactivity was analyzed in this text.The effective method is extracting with 70% ethanol,breaking with ultrasonic,extracting with n-butyl alcohol,and then using different concentrations of ethanol for elution from polyamide column.The results of the experiments indicated that the crude extract,the n-butyl alcohol extraction phase and the elution showed obviously antibacterial activity to Escherichia coli,Staphylococcus aureus and Pseudomonas aeruginosa. The extracts in this text of bitter melon obviously cleared the H_2O_2. Qualitative analysis showed that the extracts contained saponins,flavonoids,polyphenols and organic acids.
引文
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[5]张炳祯,彭艳丽,李亮.苦瓜的现代研究进展[J].食品与药品,2006,8(4):26-30.
[6]尤玲玲,陈永慧,刘金福,等.苦瓜皂苷对胰岛素抵抗Hep G2细胞葡萄糖消耗量的影响[J].食品工业科技,2014,35(5):338-340,345.
[7]尤玲玲,刘金福,王媛,等.苦瓜皂苷的纯化及降血糖活性的研究[J].食品研究与开发,2015,36(17):32-35.
[8]李毅腾,王晓闻,何庆峰,等.苦瓜皂苷与南瓜多糖或苦荞黄酮联合作用改善2型糖尿病大鼠糖脂代谢的研究[J].现代食品科技.2015,31(9):14-20.
[9]贺莹,韩慧,王丽华,等.苦瓜活性物质的研究[J].河北工业大学成人教育学院学报,2008,23(2):28-31.
[10]洪志伟,孟令宇,葛雅琨,等.槲皮素对大肠杆菌和金黄色葡萄球菌的体外抑菌作用的研究[J].吉林化工学院学报,2017,34(5):38-41.
[11]杜文静,谢亚斌,葛雅琨,等.水飞蓟宾对金黄色葡萄球菌和大肠杆菌的体外抑菌作用的研究[J].吉林化工学院学报,2016,33(7):66-69.
[2]向长萍,吴昌银,汪李平.苦瓜营养成分分析及利用评价[J].华中农业大学学报,2000,19(4):388-390.
[3]侣丽红,赵余庆.苦瓜中降血糖活性成分的提取分离与鉴定[J].中药材,2004(12):922-923.
[4]靳学远.苦瓜的化学成分及功能作用研究进展[J].山西食品工业,2005(2):38-40.
[5]张炳祯,彭艳丽,李亮.苦瓜的现代研究进展[J].食品与药品,2006,8(4):26-30.
[6]尤玲玲,陈永慧,刘金福,等.苦瓜皂苷对胰岛素抵抗Hep G2细胞葡萄糖消耗量的影响[J].食品工业科技,2014,35(5):338-340,345.
[7]尤玲玲,刘金福,王媛,等.苦瓜皂苷的纯化及降血糖活性的研究[J].食品研究与开发,2015,36(17):32-35.
[8]李毅腾,王晓闻,何庆峰,等.苦瓜皂苷与南瓜多糖或苦荞黄酮联合作用改善2型糖尿病大鼠糖脂代谢的研究[J].现代食品科技.2015,31(9):14-20.
[9]贺莹,韩慧,王丽华,等.苦瓜活性物质的研究[J].河北工业大学成人教育学院学报,2008,23(2):28-31.
[10]洪志伟,孟令宇,葛雅琨,等.槲皮素对大肠杆菌和金黄色葡萄球菌的体外抑菌作用的研究[J].吉林化工学院学报,2017,34(5):38-41.
[11]杜文静,谢亚斌,葛雅琨,等.水飞蓟宾对金黄色葡萄球菌和大肠杆菌的体外抑菌作用的研究[J].吉林化工学院学报,2016,33(7):66-69.