道地药材川麦冬和浙麦冬的生态遗传分化
|
摘要
目的:探讨川麦冬和浙麦冬的生态型差异,为道地药材生物学实质的深入研究提供参考。方法:选择传统道地药材麦冬为模型,收集75份实验样本,通过分子鉴定技术和生态因子分析研究川麦冬和浙麦冬的生态型差异。结果:川麦冬和浙麦冬的psbA-trnH序列在49位点处存在变异,川麦冬为碱基腺嘌呤(A),浙麦冬为碱基鸟嘌呤(G),此为两者序列的差异化特征;生态因子分析显示四川、浙江两大产区呈现不同的地理生态型。其中,浙江产区在年均日照强度、年均降水量、年均相对湿度、最热季平均温度、生长期平均温度等因子上高于四川,而四川产区的最冷季平均温度高于浙江。结论:川麦冬和浙麦冬的基因型和生态因子均存在明显差异。
Objective: To investigate the ecotype difference between Ophiopogonis Radix in Sichuan and Zhejiang province, and to provide a reference for the further study of the biological essence of geoauthentic medicinal materials. Method: Based on the traditional Chinese medicinal herbs of Ophiopogonis Radix,including75 samples,the ecological difference between Ophiopogonis Radix in Sichuan and Zhejiang province was studied by molecular identification and ecological factors analysis. Result: It can be found that a stable variation existed at the 49 site in the psb A-trn H sequence of Ophiopogonis Radix in Sichuan and Zhejiang province, Ophiopogonis Radix in Sichuan as base adenine( A) and Ophiopogonis Radix in Zhejiang as base guanine( G),this feature was both sequence difference. Ecological factor analysis showed that Sichuan and Zhejiang two regions had different geographical climate ecotype. Conclusion: There are obvious differences in genotype and ecological factors between Ophiopogonis Radix in Sichuan and Zhejiang province.
Objective: To investigate the ecotype difference between Ophiopogonis Radix in Sichuan and Zhejiang province, and to provide a reference for the further study of the biological essence of geoauthentic medicinal materials. Method: Based on the traditional Chinese medicinal herbs of Ophiopogonis Radix,including75 samples,the ecological difference between Ophiopogonis Radix in Sichuan and Zhejiang province was studied by molecular identification and ecological factors analysis. Result: It can be found that a stable variation existed at the 49 site in the psb A-trn H sequence of Ophiopogonis Radix in Sichuan and Zhejiang province, Ophiopogonis Radix in Sichuan as base adenine( A) and Ophiopogonis Radix in Zhejiang as base guanine( G),this feature was both sequence difference. Ecological factor analysis showed that Sichuan and Zhejiang two regions had different geographical climate ecotype. Conclusion: There are obvious differences in genotype and ecological factors between Ophiopogonis Radix in Sichuan and Zhejiang province.
引文
[1]廖保生,宋经元,陈士林,等.道地药材产地溯源研究[J].中国中药杂志,2014,39(20):3881-3887.
[2]孟祥才,陈士林,王喜军,等.论道地药材及栽培产地变迁[J].中国中药杂志,2011,36(13):1687-1692.
[3]黄林芳,付娟,陈士林,等.中药材生态变异的学术探讨[J].中草药,2012,43(7):1249-1258.
[4]黄林芳,陈士林.中药品质生态学:一个新兴交叉学科[J].中国实验方剂学杂志,2017,23(1):1-11.
[5]黄林芳,索风梅,陈士林,等.中国产西洋参品质变异及生态型划分[J].药学学报,2013,48(4):580-589.
[6]陈士林.道地药材与生态型的相关性[J].中草药,1988,10(8):40-41.
[7]清·顾观光.神农本草经[M].杨鹏举,校注.北京:学苑出版社,2007:44-46.
[8]国家中医药管理局《中华本草》编委会.中华本草[M].上海:上海科学技术出版社,1999:122-129.
[9]余伯阳.从麦冬入手,探讨中药材现代系统研究的思路与方法[J].中国天然药物,2007,5(1):10-14.
[10]龚亚君,张洁,陈家春,等.中药麦冬本草溯源疑[J].中国药师,2017,20(2):229-231.
[11]国家药典委员会.中华人民共和国药典.一部[M].北京:中国医药科技出版社,2015:26,155.
[12]童菊华,王玉霞,孙虹,等.基于代谢组学技术的杭麦冬和川麦冬药效差异性研究[J].药学学报,2014,49(10):1451-1446.
[13]陈有根,邬国庆,李慧,等.麦冬指纹图谱研究[J].中国中医药信息杂志,2008,15(4):56-58.
[14]LIN Y,ZHU D,QI J,et al.Characterization of homoisoflavonoids in different cultivation regions of Ophiopogon japonicus and related antioxidant activity[J].J Pharm Biomed Anal,2010,52(5):757-762.
[15]王翰韬,吴帆,倪穗,等.不同地域川麦冬几种活性成分比较[J].中国野生植物资源,2017,36(1):26-29.
[16]吴发明,杨瑞山,李敏,等.麦冬主流品种药材质量比较研究[J].中国药学杂志,2017,52(6):447-451.
[17]LI G,Ra W H,Park J W,et al.Developing EST-SSR markers to study molecular diversity in Liriope and Ophiopogon[J].Biochem Syst Ecol,2011,39(4/6):241-252.
[18]XIE T,LIANG Y,HAO H P,et al.Rapid identification of ophiopogonins and ophiopogonones in Ophiopogon japonicus extract with a practical technique of mass defect filtering based on high resolution mass spectrometry[J].J Chromatogr A,2012,1227:234-244.
[19]CHEN S L,PANG X H,SONG J Y,et al.A renaissance in herbal medicine identification:from morphology to DNA[J].Biotechnol Adv,2014,32(7):1237-1244.
[20]陈士林.中国药典中药材DNA条形码标准序列[M].北京:科学出版社,2015:3-23.
[21]XIN T Y,YAO H,CHEN S L,et al.Super food Lycium barbarum(Solanaceae)traceability via an internal transcribed spacer 2 barcode[J].Food Res Int,2013,54(2):1699-1704.
[22]HOU D Y,SONG J Y,YAO H,et al.Molecular identification of Corni Fructus and its adulterants by ITS/ITS2 sequences[J].Chin J Nat Med,2013,11(2):121-127.
[23]CHEN S L,YAO H,HAN J P,et al.Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plant species[J].PLo S One,2010,5(1):e8613.
[24]王俊,刘霞,陈士林,等.名贵中药材血竭的DNA条形码鉴定研究[J].中国药学杂志,2015,50(15):1261-1265.
[25]黄林芳,郑司浩,武拉斌,等.基于化学成分及分子特征中药材肉苁蓉生态型研究[J].中国科学:生命科学,2014,44(3):318-328.
[26]ZHENG S,JIANG X,CHEN S L,et al.Chemical and genetic discrimination of Cistanches Herba based on UPLC-QTOF/MS and DNA barcoding[J].PLo S One,2014,9(5):e9806.
[27]孟祥霄,黄林芳,陈士林,等.三七全球产地适宜性及品质生态学研究[J].药学学报,2016,51(9):1483-1493.
[2]孟祥才,陈士林,王喜军,等.论道地药材及栽培产地变迁[J].中国中药杂志,2011,36(13):1687-1692.
[3]黄林芳,付娟,陈士林,等.中药材生态变异的学术探讨[J].中草药,2012,43(7):1249-1258.
[4]黄林芳,陈士林.中药品质生态学:一个新兴交叉学科[J].中国实验方剂学杂志,2017,23(1):1-11.
[5]黄林芳,索风梅,陈士林,等.中国产西洋参品质变异及生态型划分[J].药学学报,2013,48(4):580-589.
[6]陈士林.道地药材与生态型的相关性[J].中草药,1988,10(8):40-41.
[7]清·顾观光.神农本草经[M].杨鹏举,校注.北京:学苑出版社,2007:44-46.
[8]国家中医药管理局《中华本草》编委会.中华本草[M].上海:上海科学技术出版社,1999:122-129.
[9]余伯阳.从麦冬入手,探讨中药材现代系统研究的思路与方法[J].中国天然药物,2007,5(1):10-14.
[10]龚亚君,张洁,陈家春,等.中药麦冬本草溯源疑[J].中国药师,2017,20(2):229-231.
[11]国家药典委员会.中华人民共和国药典.一部[M].北京:中国医药科技出版社,2015:26,155.
[12]童菊华,王玉霞,孙虹,等.基于代谢组学技术的杭麦冬和川麦冬药效差异性研究[J].药学学报,2014,49(10):1451-1446.
[13]陈有根,邬国庆,李慧,等.麦冬指纹图谱研究[J].中国中医药信息杂志,2008,15(4):56-58.
[14]LIN Y,ZHU D,QI J,et al.Characterization of homoisoflavonoids in different cultivation regions of Ophiopogon japonicus and related antioxidant activity[J].J Pharm Biomed Anal,2010,52(5):757-762.
[15]王翰韬,吴帆,倪穗,等.不同地域川麦冬几种活性成分比较[J].中国野生植物资源,2017,36(1):26-29.
[16]吴发明,杨瑞山,李敏,等.麦冬主流品种药材质量比较研究[J].中国药学杂志,2017,52(6):447-451.
[17]LI G,Ra W H,Park J W,et al.Developing EST-SSR markers to study molecular diversity in Liriope and Ophiopogon[J].Biochem Syst Ecol,2011,39(4/6):241-252.
[18]XIE T,LIANG Y,HAO H P,et al.Rapid identification of ophiopogonins and ophiopogonones in Ophiopogon japonicus extract with a practical technique of mass defect filtering based on high resolution mass spectrometry[J].J Chromatogr A,2012,1227:234-244.
[19]CHEN S L,PANG X H,SONG J Y,et al.A renaissance in herbal medicine identification:from morphology to DNA[J].Biotechnol Adv,2014,32(7):1237-1244.
[20]陈士林.中国药典中药材DNA条形码标准序列[M].北京:科学出版社,2015:3-23.
[21]XIN T Y,YAO H,CHEN S L,et al.Super food Lycium barbarum(Solanaceae)traceability via an internal transcribed spacer 2 barcode[J].Food Res Int,2013,54(2):1699-1704.
[22]HOU D Y,SONG J Y,YAO H,et al.Molecular identification of Corni Fructus and its adulterants by ITS/ITS2 sequences[J].Chin J Nat Med,2013,11(2):121-127.
[23]CHEN S L,YAO H,HAN J P,et al.Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plant species[J].PLo S One,2010,5(1):e8613.
[24]王俊,刘霞,陈士林,等.名贵中药材血竭的DNA条形码鉴定研究[J].中国药学杂志,2015,50(15):1261-1265.
[25]黄林芳,郑司浩,武拉斌,等.基于化学成分及分子特征中药材肉苁蓉生态型研究[J].中国科学:生命科学,2014,44(3):318-328.
[26]ZHENG S,JIANG X,CHEN S L,et al.Chemical and genetic discrimination of Cistanches Herba based on UPLC-QTOF/MS and DNA barcoding[J].PLo S One,2014,9(5):e9806.
[27]孟祥霄,黄林芳,陈士林,等.三七全球产地适宜性及品质生态学研究[J].药学学报,2016,51(9):1483-1493.