生长调节剂对浙麦冬种子萌发及无菌苗增殖和生根的影响
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摘要
本文研究了不同的生长调节剂对浙麦冬种子的萌发及无菌苗增殖生根的影响。实验结果表明:MS培养基中加入240 mg/L GA_3或者120 mg/L GA_3和240 mg/L 6-BA联用时,浙麦冬的种子发芽率和发芽势最高。通过浙麦冬种子发芽得到浙麦冬的无菌苗,无菌苗在6-BA浓度为3mg/L和NAA为0.5 mg/L的MS培养基中培养时其增殖系数最大;在1/2MS培养基中添加NAA浓度为0.5 mg/L平均生根数最多最好。通过浙麦冬繁殖技术的研究,以期为浙麦冬优良种苗的快速获得提供技术支持。
In this paper,the effects of different growth regulators on the seed germination and the sterile seedling's proliferating and rooting were studied by Ophiopogon japonicus. The results showed that the seed germination rate and germination potential of Ophiopogon japonicus were the highest when 240 mg/L GA_3 or 120 mg/L GA_3 and 240 mg/L 6-BAwas added to MS medium. The seed germination obtained the sterile seedling by Ophiopogon japonicus,aseptic seedlings in the 6-BA concentration of 3 mg/L and NAA for 0. 5 mg/L MS medium the proliferation coefficient; on the 1/2 MS medium with the concentration of NAA was 0. 5 mg/L the average number of rooting up to the best. Through the research on the propagation technology of Ophiopogon japonicus,we hope to provide the technical support for the rapid development of the fine seedling of Ophiopogon japonicus.
In this paper,the effects of different growth regulators on the seed germination and the sterile seedling's proliferating and rooting were studied by Ophiopogon japonicus. The results showed that the seed germination rate and germination potential of Ophiopogon japonicus were the highest when 240 mg/L GA_3 or 120 mg/L GA_3 and 240 mg/L 6-BAwas added to MS medium. The seed germination obtained the sterile seedling by Ophiopogon japonicus,aseptic seedlings in the 6-BA concentration of 3 mg/L and NAA for 0. 5 mg/L MS medium the proliferation coefficient; on the 1/2 MS medium with the concentration of NAA was 0. 5 mg/L the average number of rooting up to the best. Through the research on the propagation technology of Ophiopogon japonicus,we hope to provide the technical support for the rapid development of the fine seedling of Ophiopogon japonicus.
引文
[1]国家药典委员会.中国药典(一部)[S].北京:中国医药科技出版社,2010:289.
[2]TIAN Youqing,KOU Junping.Anti-inflammatory effects of aqueous extract from Radix Liriope muscari and its major active fraction and component[J].Chin J Nat Med,2011,9(3):222-226.
[3]LIN Y N,ZHU D N,QI J,et al.Characterization of homoisoflavonoids in different cultivation regions of Ophiopogon japonicas and related antioxidant activity[J].J Pharm Biomed Anal,2010,52(5):757-762.
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[5]CHENG Z H,WU T,GUO Y L,et al.Two new steroidal glycosides from Liriope muscari[J].Chinese Chemical Letters,2006,17(1):31-34.
[6]EUNKYOO O,SHINJIROY,YUJI K,et a1.Light activates the degradation of PIL 5 protein to promote seed germination through gibberellin in Arabidopsis[J].The Plant Journal,2006,47:124=139.
[7]HASEGAWA H,SUNG J H,MATSUMIYA S,et al.Main geinseng saponion metabolites formed by intestinal bacteria[J].Plant Med,1996,62(5):453-457.
[8]韩晶宏,史宝胜,李淑晓.6-BA和GA3浸种对麦冬种子萌发及幼苗生长的影响[J].江苏农业科学,2011(4):189-190.
[9]莫肖蓉,朱诚,任晓米,等.金边阔叶麦冬(Liriope platyphylla Wang et Tang var.variegata Hort.)的组织培养[J].植物资源与环境学报,2000(4):27-29.
[10]张俊,孙鹏,周丽莉,等.川麦冬组织培养技术研究[J].中国农学通报,2009,(24):88-91.
[11]王蔚,高山林,刘蓁,等.川麦冬脱病毒和组织培养技术的研究[J].药物生物技术,2006(4):274-278.
[2]TIAN Youqing,KOU Junping.Anti-inflammatory effects of aqueous extract from Radix Liriope muscari and its major active fraction and component[J].Chin J Nat Med,2011,9(3):222-226.
[3]LIN Y N,ZHU D N,QI J,et al.Characterization of homoisoflavonoids in different cultivation regions of Ophiopogon japonicas and related antioxidant activity[J].J Pharm Biomed Anal,2010,52(5):757-762.
[4]SHIBA M,YAMAJI H,KONDO K,et al.Discrimination of Maidong derived from Ophiopogon and Liriope species by rbc L sequence and their chemical components and tuber anatomy[J].Natural Medicines,2004,58(1):15-21.
[5]CHENG Z H,WU T,GUO Y L,et al.Two new steroidal glycosides from Liriope muscari[J].Chinese Chemical Letters,2006,17(1):31-34.
[6]EUNKYOO O,SHINJIROY,YUJI K,et a1.Light activates the degradation of PIL 5 protein to promote seed germination through gibberellin in Arabidopsis[J].The Plant Journal,2006,47:124=139.
[7]HASEGAWA H,SUNG J H,MATSUMIYA S,et al.Main geinseng saponion metabolites formed by intestinal bacteria[J].Plant Med,1996,62(5):453-457.
[8]韩晶宏,史宝胜,李淑晓.6-BA和GA3浸种对麦冬种子萌发及幼苗生长的影响[J].江苏农业科学,2011(4):189-190.
[9]莫肖蓉,朱诚,任晓米,等.金边阔叶麦冬(Liriope platyphylla Wang et Tang var.variegata Hort.)的组织培养[J].植物资源与环境学报,2000(4):27-29.
[10]张俊,孙鹏,周丽莉,等.川麦冬组织培养技术研究[J].中国农学通报,2009,(24):88-91.
[11]王蔚,高山林,刘蓁,等.川麦冬脱病毒和组织培养技术的研究[J].药物生物技术,2006(4):274-278.