无瓣海桑果实提取物对D-半乳糖致衰老小鼠抗氧化能力的影响
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摘要
目的:研究无瓣海桑(Sonneratiaapetala)果实提取物对D-半乳糖致衰老小鼠抗氧化能力的影响。方法:选用D-半乳糖(D-gal)诱导建立衰老小鼠模型,并分别灌胃无瓣海桑果实醇提物、乙酸乙酯提取物和正丁醇提取物,观察无瓣海桑果实各提取物对衰老小鼠血清、心脏和肝脏组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)的影响。结果:与模型组相比,无瓣海桑果实提取物可以有效提高衰老小鼠体内SOD、GSH-Px酶活力,降低MDA含量(P <0.05)。结论:无瓣海桑果实提取物能有效提高D-半乳糖致衰老小鼠体内抗氧化能力从而达到延缓衰老的作用。
To study the effect of extracts of Sonneratiaapetala fruit on the antioxidant capacity of aging mice induced by D-galactose. D-galactose(D-gal) was selected to establish a mouse model of aging. The aged mice were fed with extracts of Sonneratiaapetala fruit, alcohol extracts, and ethyl acetate extracts respectively. The effects of extracts on superoxide dismutase(SOD), glutathione peroxidase(GSH-Px) and malondialdehyde(MDA) in serum, heart and liver of aging mice were observed. The experimental results show that, compared with the model group, the extract of Sonneratiaapetala fruit can effectively increase the activity of SOD and GSH-Px enzymes and reduce the content of MDA in aging mice(P <0.05). The results showed that the extract of Sonneratiaapetala fruit extract can effectively improve the anti-oxidation ability of aging mice induced by D-galactose, so as to achieve the effect of delaying aging.
To study the effect of extracts of Sonneratiaapetala fruit on the antioxidant capacity of aging mice induced by D-galactose. D-galactose(D-gal) was selected to establish a mouse model of aging. The aged mice were fed with extracts of Sonneratiaapetala fruit, alcohol extracts, and ethyl acetate extracts respectively. The effects of extracts on superoxide dismutase(SOD), glutathione peroxidase(GSH-Px) and malondialdehyde(MDA) in serum, heart and liver of aging mice were observed. The experimental results show that, compared with the model group, the extract of Sonneratiaapetala fruit can effectively increase the activity of SOD and GSH-Px enzymes and reduce the content of MDA in aging mice(P <0.05). The results showed that the extract of Sonneratiaapetala fruit extract can effectively improve the anti-oxidation ability of aging mice induced by D-galactose, so as to achieve the effect of delaying aging.
引文
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6邱玉朗,万伶俐,刘海燕,等.维生素E和有机硒对小鼠生长性能、应激时抗氧化指标的影响.黑龙江畜牧兽医,2010,21:125-126.
7张延英,李广远,吴红彦,等.锁阳蜜对小鼠抗应激及抗衰老的影响.中国实验方剂学杂志,2010,16(2):33-34.
8何志坚,施旻,刘海云.菟丝子提取D-半乳糖所致衰老小鼠的抗衰老作用.中国老年学杂志,2015,35(19):5444-5446.
9王岚,梁日欣,杨滨,等.黄芩及红花水提物对快速老化模型小鼠的抗衰老作用研究.中国实验方剂学杂志,2006,16(13):159-161.
10邓秀婷,罗姮,李彦彬,等.桑葚提取液D-半乳糖致衰老小鼠抗氧化能力的影响.中国老年学杂志,2016,36(1):36-37.
2林海生,宋文东.无瓣海桑叶子和果实中氨基酸成分的检测分析.氨基酸和生物资源,2009,31(2):76-78.
3林海生,宋文东,吴婕,等.无瓣海桑叶子和果实中脂肪酸成分的检测分析.福建分析测试,2009,18(3):5-9.
4 Patra J K,Das S K,Thatoi H.Phytochemical profiling and bioactivity of a mangrove plant,Sonneratiaapetala,from Odisha Coast of India.Chin JIntegr Med,2015,21(4):274-285.
5易湘茜,李家怡,高程海,等.无瓣海桑果实乙醇提取物及其不同极性萃取物抗氧化活性.食品工业科技,2017,38(19):27-30.
6邱玉朗,万伶俐,刘海燕,等.维生素E和有机硒对小鼠生长性能、应激时抗氧化指标的影响.黑龙江畜牧兽医,2010,21:125-126.
7张延英,李广远,吴红彦,等.锁阳蜜对小鼠抗应激及抗衰老的影响.中国实验方剂学杂志,2010,16(2):33-34.
8何志坚,施旻,刘海云.菟丝子提取D-半乳糖所致衰老小鼠的抗衰老作用.中国老年学杂志,2015,35(19):5444-5446.
9王岚,梁日欣,杨滨,等.黄芩及红花水提物对快速老化模型小鼠的抗衰老作用研究.中国实验方剂学杂志,2006,16(13):159-161.
10邓秀婷,罗姮,李彦彬,等.桑葚提取液D-半乳糖致衰老小鼠抗氧化能力的影响.中国老年学杂志,2016,36(1):36-37.