中医药干预心力衰竭心肌线粒体评述
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摘要
线粒体是一类高度活跃的细胞器,为机体提供主要的能量来源。心脏是机体耗能最大的器官,心肌细胞中线粒体含量明显高于其他细胞。心力衰竭代偿期心肌线粒体代偿性增大、增多,失代偿期随着能量供应不足及代谢产物的积聚,线粒体结构和功能受损,加快心力衰竭的进程。而中医药能够通过提高过氧化物酶体增殖物激活受体γ辅激活因子-1α的表达、调控心肌线粒体能量代谢相关蛋白的表达、升高心肌线粒体膜电位、抑制线粒体通透性转变孔的开放和心肌细胞凋亡、调节心肌细胞内外Ca2+浓度、保护心肌线粒体DNA结构以及抗氧化应激等机制,多靶点、多途径保护线粒体的结构与功能,减轻心力衰竭的发生发展。
Mitochondria are a class of highly active organelles that provide the body with a major source of energy. Heart is the organ that consumes the most energy. and the mitochondrial content in cardiac cells are higher than that in other cells obviously. During the compensatory period of heart failure,myocardial mitochondria increased,and with the insufficiency of energy supply and accumulation of metabolites,mitochondrial structure and function were damaged. The process of heart failure aggravated. By increasing the expression of PGC-1α,Chinese medicine can regulate the expression of myocardial mitochondrial energy metabolism-related proteins,increase myocardial mitochondrial membrane potential,inhibit the opening of mitochondrial permeability transition pores and myocardial apoptosis,regulate the concentration of Ca2 +inside and outside the cardiomyocytes,protect the myocardial mitochondrial DNA structure and anti-oxidative stress and other mechanisms,protect the structure and function of mitochondria multitargetly and multi-pathwayly,reduce the occurrence and development of heart failure.
Mitochondria are a class of highly active organelles that provide the body with a major source of energy. Heart is the organ that consumes the most energy. and the mitochondrial content in cardiac cells are higher than that in other cells obviously. During the compensatory period of heart failure,myocardial mitochondria increased,and with the insufficiency of energy supply and accumulation of metabolites,mitochondrial structure and function were damaged. The process of heart failure aggravated. By increasing the expression of PGC-1α,Chinese medicine can regulate the expression of myocardial mitochondrial energy metabolism-related proteins,increase myocardial mitochondrial membrane potential,inhibit the opening of mitochondrial permeability transition pores and myocardial apoptosis,regulate the concentration of Ca2 +inside and outside the cardiomyocytes,protect the myocardial mitochondrial DNA structure and anti-oxidative stress and other mechanisms,protect the structure and function of mitochondria multitargetly and multi-pathwayly,reduce the occurrence and development of heart failure.
引文
[1]林谦,农一兵,黄启福.从心肌能量代谢探讨慢性心力衰竭心气虚证的实质[J].北京中医药大学学报,2007,30(7):445-450.
[2]申屠路媚,牟艳玲.线粒体功能障碍机制及其相关疾病研究进展[J].生命科学,2018,30(1):1-7.
[3]季朝红,王智慧,秦智峰,等.慢性心力衰竭心肌能量代谢重构的研究现状[J].中国循证心血管医学杂志,2017,9(8):1023-1024.
[4]史文静,周华,戎靖枫.温补心阳和肾阳方对心力衰竭大鼠心功能和心肌磷酸腺苷含量的影响[J].中西医结合心脑血管病杂志,2012,10(9):1083-1085.
[5]刘茜,周华,戎靖枫,等.温补肾阳方对心力衰竭大鼠心肌能量代谢的影响[J].中药药理与临床,2013,29(2):180-182.
[6]于春泉,李欣桐,史芳,等.芪苈强心胶囊对心气虚型慢性心力衰竭大鼠心肌腺苷酸含量的影响[J].中国实验方剂学杂志,2012,18(3):174-177.
[7]SHENG B,WANG X,SU B,et al. Impaired mitochondrial biogenesis contributes to mitochondrial dysfunction in Alzheimer's disease[J]. J Neurochem,2012,120(3):419-429.
[8]ROWE G C,JIANG A,ARANY Z. PGC-1 coactivators in cardiac development and disease[J]. Circ Res,2010,107(7):825-838.
[9]陈淑娟,汪新宇,李海燕,等. PGC-1对血管内皮细胞内线粒体生物合成的影响[J].南方医科大学学报,2010,30(5):1114-1116.
[10]曹琼丹.黄芪甲苷对Ⅰ型糖尿病大鼠心肌细胞能量代谢与凋亡的作用研究[D].锦州:锦州医科大学,2016.
[11]张艳,刘秀娜,王懿,等.冠心舒通胶囊对慢性心力衰竭大鼠心肌能量代谢的影响[J].中医杂志,2015,56(23):2054-2057.
[12]王懿,张艳,礼海.益气活血方干预PGC-1α调控心衰心肌细胞能量代谢重构的作用机制[J].中国实验方剂学杂志,2015,21(6):169-173.
[13]陈颖,陈长勋.能量代谢在心力衰竭发病机制与治疗中的研究进展[J].中成药,2014,36(6):1272-1276.
[14]NEUBAUER S. The failing heart an engine out of fuel[J]. N Engl J Med,2007,356(11):1140-1151.
[15]TUUNANEN H,KNUUTI J. Metabolic remodelling in human heart failure[J]. Cardiovasc Res,2011,90(2):251-257.
[16]苏冠华,孙雨霏,卢永昕.心力衰竭的能量代谢重构及其治疗[J].国际心血管病杂志,2012,39(2):65-67.
[17]WANG J,BAI L,LI J,et al. Proteomic analysis of mitochondria reveals a metabolic switch from fatty acid oxidation to glycolysis in the failing heart[J]. Science in China Series C-Life Science,2009,52(11):1003-1010.
[18]白玲,王军,刘平,等.芪苈强心胶囊对心力衰竭大鼠心肌线粒体蛋白质组影响的研究[J].中国分子心脏病学杂志,2014,14(3):949-952.
[19]安超,农一兵,温志浩,等.黄芪皂苷和黄芪多糖对乳鼠肥大心肌细胞线粒体膜电位的影响[J].北京中医药大学学报(中医临床版),2009,16(3):17-19.
[20]徐厚谦,孙艳,孙樱丹.当归补血汤含药血清对血管紧张素Ⅱ诱导肥大心肌细胞能量代谢的影响[J].山东中医药大学学报,2014,38(5):472-474.
[21]王红英,曹雪滨,徐淑乐,等.心复康口服液对心衰大鼠线粒体跨膜电位及心肌能量代谢的影响[J].第四军医大学学报,2009,30(19):1875-1878.
[22]谢世阳,王幼平,王小晓,等.参附益心颗粒对心力衰竭大鼠心肌ATP含量及解偶联蛋白-2的影响[J].中华中医药杂志,2017,32(10):4412-4415.
[23]GUSTAFSSON A B,GOTTLIEB R A. Heart mitochondria:gates of life and death[J]. Cardiovasc Res,2008,77(2):334-343.
[24]赵淑琴,孙蒙,陈慧颖,等.衰老大鼠心肌线粒体PT孔开放对线粒体呼吸功能的影响[J].中国老年学杂志,2015,2(35):404-406.
[25]唐伦先,许明正,张颖,等.线粒体通透性转换孔在慢性心力衰竭中的作用及n-3PUFA的保护机制[J].同济大学学报(医学版),2016,37(4):1-6.
[26]李海涛.抑制线粒体膜通透性转换孔开放对心力衰竭的影响[J].安徽医科大学学报,2013,48(10):1167-1169.
[27]王陵军,冼绍祥,高梦夕,等.益气温阳活血法对心力衰竭大鼠心肌细胞线粒体通透性转变的影响[J].中药新药与临床药理,2014,25(3):276-279.
[28]赵欣梅,张玉彬.细胞凋亡与心力衰竭[J].药学研究,2017,36(8):467-470.
[29]JOHNSON A,DIPIETRO L A. Apoptosis and angiogenesis:an evolving mechanism for fibrosis[J]. FASEB J,2013,27(10):3893-3901.
[30]LI Y,DAI Y B,SUN J Y,et al. Neuroglobin Attenuates Beta Amyloid-Induced Apoptosis Through Inhibiting Caspases Activity by Activating PI3K/Akt Signaling Pathway[J]. J Mol Neurosci,2016,58(1):28-38.
[31]GUPTA S K,SAHOO A P,ROSH N,et al. Canine parvovirus NS1induced apoptosis involves mitochondria,accumulation of reactive oxygen species and activation of caspases[J]. Virus Res,2016(213):46-61.
[32]焦宏,任君旭,赵兰平,等.参麦注射液对乳鼠心肌细胞线粒体凋亡通路的影响[J].中国中医基础医学杂志,2012,18(5):552-554.
[33]梅蒙,王洪新,唐富天,等.黄芪甲苷对异丙肾上腺素诱导的H9C2心肌细胞凋亡的影响[J].中药药理与临床,2015,31(5):37-41.
[34]XIONG Y,ZHANG M,CHEN F,et al. Roles of mitochondrial dysfunction in cardiovascular diseases[J]. Chn J Pathophysiol,2013,29(3):364-370.
[35]郭鑫,于天源,周嫱,等.肌肉疲劳及肌肉损伤机制研究综述.中华中医药杂志,2016,31(7):2720-2724.
[36]李兵,柳君泽,陈丽芬.缺氧对大鼠心肌线粒体能量代谢和腺苷酸转位酶活性的影响[J].中国病理生理杂志,2006,22(3):460-463.
[37]苗梦露,李七一,严士海,等.抗心衰颗粒对舒张性心衰大鼠模型心肌细胞内钙离子、ATP酶及心肌线粒体超微结构的影响[J].北京中医药大学学报,2013,36(7):468-475.
[38]李永民,王保和,贺金,等.和营安心方对慢性心衰大鼠心功能及心肌细胞内钙离子浓度的影响[J].中华中医药杂志,2009,24(4):536-538.
[39]苏丹,严浩然,张蓓蓓,等.黄芪对慢性心衰大鼠心功能及心肌肌浆网钙泵基因表达的影响[J].中药材,2009,32(1):85-88.
[40]刘喜平,李沛清,刘勍,吴红彦.参参康心胶囊对小鼠缺血心肌线粒体超微结构及Na+、Mg2+、Ca2+含量的影响[J].中成药,2011,33(2):329-331.
[41]SHOKOLENKO I,VENEDIKTOVA N,BOCHKAREVA A,et al.Oxidative stress induces degradation of mitochondrial DNA[J]. Nucleic Acids Res,2009,37:2539-2548.
[42]袁祖贻,刘治全,马爱群,等.心力衰竭患者心肌线粒体DNA缺失突变的定量分析及与心功能损害程度的关系[J].中华心血管病杂志,1998,17(1):45-47.
[43]赵建业,顾翔. mt DNA的遗传学特征与心血管疾病[J].国际心血管病杂志,2010,37(6):339-341,356.
[44]MARIN-GARCIA J,GOLDENTHAL M J. Cardiomyopathy and abnormal mitochondrial function[J]. Cardiovasc Res,1994,28:456-461.
[45]KARAMANLIDIS G,BAUTISTA-HERNANDEZ V,FYNNTHOMPSON F,et al. Impaired mitochondrial biogenesis precedes heart failure in right ventricular hypertrophy in congenital heart disease[J]. Circ Heart Fail,2011,4(6):707-713.
[46]袁祖贻,刘治全,马爱群,等.心力衰竭患者心肌线粒体脱氧核糖核酸损伤与衰竭心肌线粒体酶活性的关系[J].中国循环杂志,2000,15(5):285-286.
[47]戚艳超,徐明.心肌细胞超微机构改变与心力衰竭[J].中国医学前沿杂志,2013,5(4):27-31.
[48]刘剑刚,董国菊,史大卓,等.愈心梗液保护急性心肌梗死后心力衰竭大鼠心肌细胞和线粒体超微结构及抗氧化作用的实验研究[J].中国微循环,2005,9(6):389-392.
[49]王显,朱明军,孙建芝,等.“心衰康”对电镜下CHF模型心肌线粒体的影响[J].上海中医药大学学报,2000(1):33-35.
[50]康玉明,李祥,李宏宝.心力衰竭中枢发病机制的研究进展[J].西安交通大学学报(医学版),2017,38(2):157-160.
[51]MAACK C,BOHM M. Targeting mitochondrial oxidative stress in heart failure throttling the afterburner[J]. J Am Coll Cardiol,2011,58(1):83-86.
[52]ZANG Q S,SADEK H,MAASS D L,et al. Specific inhibition of mitochondrial oxidative stress suppresses inflammation and improves cardiac function in a rat pneumonia-related sepsis model[J]. Am J Physiol Heart Circ Physiol,2012,302(9):1847-1859.
[53]王金华,车頔,陈东,等.醒脑静注射液对脓毒症大鼠心肌线粒体氧化应激损伤的作用[J].实用医学杂志,2015,31(9):1403-1406.
[54]孙娜,徐钢,张凡,等.四君子汤(生晒参/红参)对H2O2诱导H9c2心肌细胞凋亡的保护作用研究[J].中国中药杂志,2015,40(10):1989-1993.
[55]范宗静,谢连娣,董巧稚,等.黄芪多糖抗心肌缺血再灌注损伤的线粒体机制研究[J].中国中医基础医学杂志,2017,23(4):484-487.
[56]李锦鸣,吕允,郝敬红,等.吕光荣教授针药并用治疗慢性心衰临床经验探析[J].针灸临床杂志,2013,29(5):75-76.
[57]张其梅,张富亮,李国政.活血温阳汤合针灸治疗慢性心力衰竭疗效观察[J].辽宁中医杂志,2008,35(6):877-878.
[58]李金波,满斌.针刺治疗无症状心力衰竭及其对患者左室收缩功能的影响[J].中国针灸,2004,24(5):303-305.
[59]邓元江,梁伟雄,程淑意.穴位埋线对慢性心力衰竭大鼠血压与心功能的影响[J].针刺研究,2011,36(1):40-45.
[60]胡杨武.针刺列缺、内关穴治疗急性左心衰[J].湖南中医药导报,1997,3(2/3):102.
[61]王华,邓丽霞,吴绪平,等.电针“内关”对室性心动过速大鼠心率及血浆儿茶酚胺含量的影响[J].针刺研究,2009,34(3):180-182,187.
[62]涂杰,李涛,梁东科,等.电针内关穴对大鼠体外循环后心肌线粒体超微结构及氧化应激的影响[J].广东医学,2015,36(9):1322-1326.
[63]谢俊,陈泽斌,吴松,等.标本配穴法电针对慢性心肌缺血大鼠心肌线粒体结构及线粒体DNA相关调控因子表达的影响[J].中华中医药杂志,2017,32(3):1251-1255.
[2]申屠路媚,牟艳玲.线粒体功能障碍机制及其相关疾病研究进展[J].生命科学,2018,30(1):1-7.
[3]季朝红,王智慧,秦智峰,等.慢性心力衰竭心肌能量代谢重构的研究现状[J].中国循证心血管医学杂志,2017,9(8):1023-1024.
[4]史文静,周华,戎靖枫.温补心阳和肾阳方对心力衰竭大鼠心功能和心肌磷酸腺苷含量的影响[J].中西医结合心脑血管病杂志,2012,10(9):1083-1085.
[5]刘茜,周华,戎靖枫,等.温补肾阳方对心力衰竭大鼠心肌能量代谢的影响[J].中药药理与临床,2013,29(2):180-182.
[6]于春泉,李欣桐,史芳,等.芪苈强心胶囊对心气虚型慢性心力衰竭大鼠心肌腺苷酸含量的影响[J].中国实验方剂学杂志,2012,18(3):174-177.
[7]SHENG B,WANG X,SU B,et al. Impaired mitochondrial biogenesis contributes to mitochondrial dysfunction in Alzheimer's disease[J]. J Neurochem,2012,120(3):419-429.
[8]ROWE G C,JIANG A,ARANY Z. PGC-1 coactivators in cardiac development and disease[J]. Circ Res,2010,107(7):825-838.
[9]陈淑娟,汪新宇,李海燕,等. PGC-1对血管内皮细胞内线粒体生物合成的影响[J].南方医科大学学报,2010,30(5):1114-1116.
[10]曹琼丹.黄芪甲苷对Ⅰ型糖尿病大鼠心肌细胞能量代谢与凋亡的作用研究[D].锦州:锦州医科大学,2016.
[11]张艳,刘秀娜,王懿,等.冠心舒通胶囊对慢性心力衰竭大鼠心肌能量代谢的影响[J].中医杂志,2015,56(23):2054-2057.
[12]王懿,张艳,礼海.益气活血方干预PGC-1α调控心衰心肌细胞能量代谢重构的作用机制[J].中国实验方剂学杂志,2015,21(6):169-173.
[13]陈颖,陈长勋.能量代谢在心力衰竭发病机制与治疗中的研究进展[J].中成药,2014,36(6):1272-1276.
[14]NEUBAUER S. The failing heart an engine out of fuel[J]. N Engl J Med,2007,356(11):1140-1151.
[15]TUUNANEN H,KNUUTI J. Metabolic remodelling in human heart failure[J]. Cardiovasc Res,2011,90(2):251-257.
[16]苏冠华,孙雨霏,卢永昕.心力衰竭的能量代谢重构及其治疗[J].国际心血管病杂志,2012,39(2):65-67.
[17]WANG J,BAI L,LI J,et al. Proteomic analysis of mitochondria reveals a metabolic switch from fatty acid oxidation to glycolysis in the failing heart[J]. Science in China Series C-Life Science,2009,52(11):1003-1010.
[18]白玲,王军,刘平,等.芪苈强心胶囊对心力衰竭大鼠心肌线粒体蛋白质组影响的研究[J].中国分子心脏病学杂志,2014,14(3):949-952.
[19]安超,农一兵,温志浩,等.黄芪皂苷和黄芪多糖对乳鼠肥大心肌细胞线粒体膜电位的影响[J].北京中医药大学学报(中医临床版),2009,16(3):17-19.
[20]徐厚谦,孙艳,孙樱丹.当归补血汤含药血清对血管紧张素Ⅱ诱导肥大心肌细胞能量代谢的影响[J].山东中医药大学学报,2014,38(5):472-474.
[21]王红英,曹雪滨,徐淑乐,等.心复康口服液对心衰大鼠线粒体跨膜电位及心肌能量代谢的影响[J].第四军医大学学报,2009,30(19):1875-1878.
[22]谢世阳,王幼平,王小晓,等.参附益心颗粒对心力衰竭大鼠心肌ATP含量及解偶联蛋白-2的影响[J].中华中医药杂志,2017,32(10):4412-4415.
[23]GUSTAFSSON A B,GOTTLIEB R A. Heart mitochondria:gates of life and death[J]. Cardiovasc Res,2008,77(2):334-343.
[24]赵淑琴,孙蒙,陈慧颖,等.衰老大鼠心肌线粒体PT孔开放对线粒体呼吸功能的影响[J].中国老年学杂志,2015,2(35):404-406.
[25]唐伦先,许明正,张颖,等.线粒体通透性转换孔在慢性心力衰竭中的作用及n-3PUFA的保护机制[J].同济大学学报(医学版),2016,37(4):1-6.
[26]李海涛.抑制线粒体膜通透性转换孔开放对心力衰竭的影响[J].安徽医科大学学报,2013,48(10):1167-1169.
[27]王陵军,冼绍祥,高梦夕,等.益气温阳活血法对心力衰竭大鼠心肌细胞线粒体通透性转变的影响[J].中药新药与临床药理,2014,25(3):276-279.
[28]赵欣梅,张玉彬.细胞凋亡与心力衰竭[J].药学研究,2017,36(8):467-470.
[29]JOHNSON A,DIPIETRO L A. Apoptosis and angiogenesis:an evolving mechanism for fibrosis[J]. FASEB J,2013,27(10):3893-3901.
[30]LI Y,DAI Y B,SUN J Y,et al. Neuroglobin Attenuates Beta Amyloid-Induced Apoptosis Through Inhibiting Caspases Activity by Activating PI3K/Akt Signaling Pathway[J]. J Mol Neurosci,2016,58(1):28-38.
[31]GUPTA S K,SAHOO A P,ROSH N,et al. Canine parvovirus NS1induced apoptosis involves mitochondria,accumulation of reactive oxygen species and activation of caspases[J]. Virus Res,2016(213):46-61.
[32]焦宏,任君旭,赵兰平,等.参麦注射液对乳鼠心肌细胞线粒体凋亡通路的影响[J].中国中医基础医学杂志,2012,18(5):552-554.
[33]梅蒙,王洪新,唐富天,等.黄芪甲苷对异丙肾上腺素诱导的H9C2心肌细胞凋亡的影响[J].中药药理与临床,2015,31(5):37-41.
[34]XIONG Y,ZHANG M,CHEN F,et al. Roles of mitochondrial dysfunction in cardiovascular diseases[J]. Chn J Pathophysiol,2013,29(3):364-370.
[35]郭鑫,于天源,周嫱,等.肌肉疲劳及肌肉损伤机制研究综述.中华中医药杂志,2016,31(7):2720-2724.
[36]李兵,柳君泽,陈丽芬.缺氧对大鼠心肌线粒体能量代谢和腺苷酸转位酶活性的影响[J].中国病理生理杂志,2006,22(3):460-463.
[37]苗梦露,李七一,严士海,等.抗心衰颗粒对舒张性心衰大鼠模型心肌细胞内钙离子、ATP酶及心肌线粒体超微结构的影响[J].北京中医药大学学报,2013,36(7):468-475.
[38]李永民,王保和,贺金,等.和营安心方对慢性心衰大鼠心功能及心肌细胞内钙离子浓度的影响[J].中华中医药杂志,2009,24(4):536-538.
[39]苏丹,严浩然,张蓓蓓,等.黄芪对慢性心衰大鼠心功能及心肌肌浆网钙泵基因表达的影响[J].中药材,2009,32(1):85-88.
[40]刘喜平,李沛清,刘勍,吴红彦.参参康心胶囊对小鼠缺血心肌线粒体超微结构及Na+、Mg2+、Ca2+含量的影响[J].中成药,2011,33(2):329-331.
[41]SHOKOLENKO I,VENEDIKTOVA N,BOCHKAREVA A,et al.Oxidative stress induces degradation of mitochondrial DNA[J]. Nucleic Acids Res,2009,37:2539-2548.
[42]袁祖贻,刘治全,马爱群,等.心力衰竭患者心肌线粒体DNA缺失突变的定量分析及与心功能损害程度的关系[J].中华心血管病杂志,1998,17(1):45-47.
[43]赵建业,顾翔. mt DNA的遗传学特征与心血管疾病[J].国际心血管病杂志,2010,37(6):339-341,356.
[44]MARIN-GARCIA J,GOLDENTHAL M J. Cardiomyopathy and abnormal mitochondrial function[J]. Cardiovasc Res,1994,28:456-461.
[45]KARAMANLIDIS G,BAUTISTA-HERNANDEZ V,FYNNTHOMPSON F,et al. Impaired mitochondrial biogenesis precedes heart failure in right ventricular hypertrophy in congenital heart disease[J]. Circ Heart Fail,2011,4(6):707-713.
[46]袁祖贻,刘治全,马爱群,等.心力衰竭患者心肌线粒体脱氧核糖核酸损伤与衰竭心肌线粒体酶活性的关系[J].中国循环杂志,2000,15(5):285-286.
[47]戚艳超,徐明.心肌细胞超微机构改变与心力衰竭[J].中国医学前沿杂志,2013,5(4):27-31.
[48]刘剑刚,董国菊,史大卓,等.愈心梗液保护急性心肌梗死后心力衰竭大鼠心肌细胞和线粒体超微结构及抗氧化作用的实验研究[J].中国微循环,2005,9(6):389-392.
[49]王显,朱明军,孙建芝,等.“心衰康”对电镜下CHF模型心肌线粒体的影响[J].上海中医药大学学报,2000(1):33-35.
[50]康玉明,李祥,李宏宝.心力衰竭中枢发病机制的研究进展[J].西安交通大学学报(医学版),2017,38(2):157-160.
[51]MAACK C,BOHM M. Targeting mitochondrial oxidative stress in heart failure throttling the afterburner[J]. J Am Coll Cardiol,2011,58(1):83-86.
[52]ZANG Q S,SADEK H,MAASS D L,et al. Specific inhibition of mitochondrial oxidative stress suppresses inflammation and improves cardiac function in a rat pneumonia-related sepsis model[J]. Am J Physiol Heart Circ Physiol,2012,302(9):1847-1859.
[53]王金华,车頔,陈东,等.醒脑静注射液对脓毒症大鼠心肌线粒体氧化应激损伤的作用[J].实用医学杂志,2015,31(9):1403-1406.
[54]孙娜,徐钢,张凡,等.四君子汤(生晒参/红参)对H2O2诱导H9c2心肌细胞凋亡的保护作用研究[J].中国中药杂志,2015,40(10):1989-1993.
[55]范宗静,谢连娣,董巧稚,等.黄芪多糖抗心肌缺血再灌注损伤的线粒体机制研究[J].中国中医基础医学杂志,2017,23(4):484-487.
[56]李锦鸣,吕允,郝敬红,等.吕光荣教授针药并用治疗慢性心衰临床经验探析[J].针灸临床杂志,2013,29(5):75-76.
[57]张其梅,张富亮,李国政.活血温阳汤合针灸治疗慢性心力衰竭疗效观察[J].辽宁中医杂志,2008,35(6):877-878.
[58]李金波,满斌.针刺治疗无症状心力衰竭及其对患者左室收缩功能的影响[J].中国针灸,2004,24(5):303-305.
[59]邓元江,梁伟雄,程淑意.穴位埋线对慢性心力衰竭大鼠血压与心功能的影响[J].针刺研究,2011,36(1):40-45.
[60]胡杨武.针刺列缺、内关穴治疗急性左心衰[J].湖南中医药导报,1997,3(2/3):102.
[61]王华,邓丽霞,吴绪平,等.电针“内关”对室性心动过速大鼠心率及血浆儿茶酚胺含量的影响[J].针刺研究,2009,34(3):180-182,187.
[62]涂杰,李涛,梁东科,等.电针内关穴对大鼠体外循环后心肌线粒体超微结构及氧化应激的影响[J].广东医学,2015,36(9):1322-1326.
[63]谢俊,陈泽斌,吴松,等.标本配穴法电针对慢性心肌缺血大鼠心肌线粒体结构及线粒体DNA相关调控因子表达的影响[J].中华中医药杂志,2017,32(3):1251-1255.