高载荷作用对大鼠椎间盘退行性变的影响
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摘要
[目的]模拟战斗机飞行员超高加速度飞行和进行特殊战术动作时的高载荷环境,探索该环境对飞行员椎间盘退行性变的结构功能影响和潜在机制。[方法]利用动物离心机制备+6 Gz组、+10 Gz组高载荷下大鼠椎间盘退行性变模型各10只,通过对大鼠体重、L5/6椎间盘MRI成像和HE染色三种方法,对退行性变的发生情况进行检测。[结果]随着载荷的增强,大鼠椎间盘含水量减少,高度丢失,在HE染色中发现纤维环结构破坏。[结论]大鼠椎间盘退行性变与高载荷影响有关,且随着载荷的升高,椎间盘退行性变加重。
[Objective] To simulate the high-load environment as fighter pilots taking ultra-high acceleration flight and special tactical actions, and to explore the influence of this environment on the structural function and potential mechanism of intervertebral disc degeneration in pilots. [Methods] This study included 30 Sprague-Dawley(SD) rats in all 3 groups. The 10 SD rats in +6 Gz and +10 Gz groups were modeling in animal centrifuge. Rat body weight, MRI imaging and HE-staining of L5-6 intervertebral disc were used for measurement and analysis. [Results] With the increase of load, the water content and height of the intervertebral disc of rats was reduced and the annulus fibrosus structure was destroyed showed in HE-staining. [Conclusion] The extent of intervertebral disc degeneration in rats is related to the load, and the degree of degeneration increases with the increase of load.
[Objective] To simulate the high-load environment as fighter pilots taking ultra-high acceleration flight and special tactical actions, and to explore the influence of this environment on the structural function and potential mechanism of intervertebral disc degeneration in pilots. [Methods] This study included 30 Sprague-Dawley(SD) rats in all 3 groups. The 10 SD rats in +6 Gz and +10 Gz groups were modeling in animal centrifuge. Rat body weight, MRI imaging and HE-staining of L5-6 intervertebral disc were used for measurement and analysis. [Results] With the increase of load, the water content and height of the intervertebral disc of rats was reduced and the annulus fibrosus structure was destroyed showed in HE-staining. [Conclusion] The extent of intervertebral disc degeneration in rats is related to the load, and the degree of degeneration increases with the increase of load.
引文
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[14]Mayer JE,Iatridis JC,Chan D,et al.Genetic polymorphisms associated with intervertebral disc degeneration[J].Spine J,2013,13:299-317.
[15]Erwin WM,DeSouza L,Funabashi M,et al.The biological basis of degenerative disc disease:proteomic and biomechanical analysis of the canine intervertebral disc[J].Arthritis Res Ther,2015,17:240.
[16]冯艺,叶忠明,郭彦祥,等.501名飞行员腰椎间盘突出症发病情况调查[J].中国疗养医学,2004(1):33-34.
[17]Cunningham LK,Docherty S,Tyler AW.Prevalence of low back pain(LBP)in rotary wing aviation pilots[J].Aviat Space Environ Med,2010,81:774-778.
[18]Truszczynska A,Lewkowicz R,Truszczynski O,et al.Back pain in Polish military helicopter pilots[J].Int J Occup Med Environ Health,2012,25:258-264.
[2]Kepler CK,Ponnappan RK,Tannoury CA,et al.The molecular basis of intervertebral disc degeneration[J].Spine J,2013,13:318-330.
[3]Zhang F,Zhao X,Shen H,et al.Molecular mechanisms of cell death in intervertebral disc degeneration(Review)[J].Int J Mol Med,2016,37:1439-1448.
[4]Livshits G,Popham M,Malkin I,et al.Lumbar disc degeneration and genetic factors are the main risk factors for low back pain in women:the UK Twin Spine Study[J].Ann Rheum Dis,2011,70:1740-1745.
[5]朱克顺,伍骥,潘庆联,等.歼击机飞行员腰椎退行性病变随访观察[J].中华航空航天医学杂志,2005:42-44,81.
[6]Hendriksen IJ,Holewijn M.Degenerative changes of the spine of fighter pilots of the Royal Netherlands Air Force(RNLAF)[J].Aviat Space Environ Med,1999,70:1057-1063.
[7]Park JS,Park JB,Park IJ,et al.Accelerated premature stress-induced senescence of young annulus fibrosus cells of rats by high glucose-induced oxidative stress[J].Int Orthop,2014,38:1311-1320.
[8]Wu Q,Huang JH.Ectopic expression of Smurf and acceleration of age-related intervertebral disc degeneration in a mouse model[J].J Neurosurg Spine,2017,27(1):116-126.
[9]程修强.改良HE染色在病理诊断中的实践研究[J].中国处方药,2018,16(2):142-143.
[10]Schollum ML,Robertson PA,Broom ND.ISSLS prize winner:microstructure and mechanical disruption of the lumbar disc annulus:part I:a microscopic investigation of the translamellar bridging network[J].Spine(Phila Pa 1976),2008,33:2702-2710.
[11]Jin L,Balian G,Li XJ.Animal models for disc degeneration-an update[J].Histol Histopathol,2018,33:543-554.
[12]Daly C,Ghosh P,Jenkin G,et al.A review of animal models of intervertebral disc degeneration:pathophysiology,regeneration,and translation to the clinic[J].Biomed Res Int,2016,2016:5952165.
[13]Jiang L,Zhang X,Zheng X,et al.Apoptosis,senescence,and autophagy in rat nucleus pulposus cells:implications for diabetic intervertebral disc degeneration[J].J Orthop Res,2013,31:692-702.
[14]Mayer JE,Iatridis JC,Chan D,et al.Genetic polymorphisms associated with intervertebral disc degeneration[J].Spine J,2013,13:299-317.
[15]Erwin WM,DeSouza L,Funabashi M,et al.The biological basis of degenerative disc disease:proteomic and biomechanical analysis of the canine intervertebral disc[J].Arthritis Res Ther,2015,17:240.
[16]冯艺,叶忠明,郭彦祥,等.501名飞行员腰椎间盘突出症发病情况调查[J].中国疗养医学,2004(1):33-34.
[17]Cunningham LK,Docherty S,Tyler AW.Prevalence of low back pain(LBP)in rotary wing aviation pilots[J].Aviat Space Environ Med,2010,81:774-778.
[18]Truszczynska A,Lewkowicz R,Truszczynski O,et al.Back pain in Polish military helicopter pilots[J].Int J Occup Med Environ Health,2012,25:258-264.