侵袭性牙周炎患者龈沟液中miR-21表达及与MMP-9水平相关性分析
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摘要
目的:探讨侵袭性牙周炎患者龈沟液中miR-21表达及与MMP-9水平的相关性。方法:本院侵袭性牙周炎患者89例为病例组,门诊健康体检者78例为对照组;测定两组龈沟液中miR-21、MMP-9水平,并检测菌斑指数、探诊深度、临床附着丧失和出血指数。结果:病例组菌斑指数、探诊深度、临床附着丧失、出血指数均高于对照组,差异有统计学意义(P<0.05);病例组MiR-21mRNA表达水平高于对照组,差异有统计学意义(P<0.05);病例组MMP-9表达水平高于对照组,差异有统计学意义(P<0.05); miRNA-21与菌斑指数、探诊深度、临床附着丧失、出血指数、MMP-9正相关关系明显,差异有统计学意义(P<0.01)。结论:侵袭性牙周炎龈沟液miR-21、MMP-9表达水平密切正相关; miR-21、MMP-9与侵袭性牙周炎组织病变情况密切相关,其在侵袭性牙周炎的发病机制中起着至关重要的作用。
Objective: To investigate the miR-21 expression levels in gingival crevicular fluid and its correlation with MMP-9 levels in patients with aggressive periodontitis. Methods: 89 cases of aggressive periodontitis (case group) and 78 cases of health individuals in our hospital (control group) were selected. Then various indicators were detected,including expression levels of microRNA-21 and MMP-9 in gingival crevicular fluid,the plaque index,probing depth,clinical attachment loss and bleeding index. Results: The plaque index,probing depth,clinical attachment loss and bleeding index of the case group were higher than those of the control group (P <0.05); The levels of microRNA-21 and MMP-9 in gingival crevicular fluid of the case group were higher than those of the control group (P <0.05); miRNA-21 was positively correlated with plaque index,probing depth,clinical attachment loss,bleeding index and MMP-9 (P <0.01). Conclusion: The expression levels of microRNA-21 and MMP-9 in gingival crevicular fluid are positively correlated,which play an important role in the pathogenesis of aggressive periodontitis.
Objective: To investigate the miR-21 expression levels in gingival crevicular fluid and its correlation with MMP-9 levels in patients with aggressive periodontitis. Methods: 89 cases of aggressive periodontitis (case group) and 78 cases of health individuals in our hospital (control group) were selected. Then various indicators were detected,including expression levels of microRNA-21 and MMP-9 in gingival crevicular fluid,the plaque index,probing depth,clinical attachment loss and bleeding index. Results: The plaque index,probing depth,clinical attachment loss and bleeding index of the case group were higher than those of the control group (P <0.05); The levels of microRNA-21 and MMP-9 in gingival crevicular fluid of the case group were higher than those of the control group (P <0.05); miRNA-21 was positively correlated with plaque index,probing depth,clinical attachment loss,bleeding index and MMP-9 (P <0.01). Conclusion: The expression levels of microRNA-21 and MMP-9 in gingival crevicular fluid are positively correlated,which play an important role in the pathogenesis of aggressive periodontitis.
引文
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[2]Munz M,Chen H,Jockel‐Schneider Y,et al.A haplotype block downstream of plasminogen is associated with chronic and aggressive periodontitis[J].Journal of Clinical Periodontology,2017,3(3):23~25.
[3]曹采方,孟焕新,阎福华,等.牙周疾病新分类简介(1999年国际研讨会)[J].中华口腔医学杂志,2001,36(5):391~393.
[4]Venugopal P,Lavu V,Rangarao S,et al.Evaluation of a panel of single-nucleotide polymorphisms in miR-146a and miR-196a2 genomic regions in patients with chronic periodontitis[J].Genetic Testing&Molecular Biomarkers,2017,21(4):56~58.
[5]Yang N,Li Y,Wang G,et al.Tumor necrosis factor-αsuppresses adipogenic and osteogenic differentiation of human periodontal ligament stem cell by inhibiting miR-21/Spry1functional axis[J].Differentiation,2017,97(3):33~35.
[6]Juneja S C,Veillette C.Defects in tendon,ligament and enthesis in response to genetic alterations in key proteoglycans and glycoproteins:a review[J].Arthritis,2013,3(1):812~815.
[7]Lauren M,Minire A,Maldi X,et al.The impact of periodontitis in the preterm birth and body size of newborns[J].Materia Socio-Medica,2012,24(1):44~47.