光照强度对狼爪瓦松愈伤组织有效物质积累的影响
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摘要
以狼爪瓦松种子诱导的愈伤组织为材料,研究了光照强度对愈伤组织生物量及黄酮积累的影响。结果表明:光照强度为15~30("mol·cm~(-2)·s~(-1))时,有利于狼爪瓦松愈伤组织鲜重和干重的增加;对于狼爪瓦松中酚的积累,光照强度为15("mol·cm~(-2)·s~(-1))时,其含量(17.4mg/g DW)及生产量(68.53mg/L)最高。而当光照强度达到30("mol·cm~(-2)·s~(-1))时,可得到最高的多糖含量(125.8 mg/g DW)和生产量(615.05mg/L)。对于黄酮类物质,光照强度为15("mol·cm~(-2)·s~(-1))时,最有利于山奈酚-3-O-芸香糖苷的积累,而槲皮素、山奈素、表儿茶素没食子酸酯和异槲皮苷的积累在光照强度为30("mol·cm~(-2)·s~(-1))时最佳;总黄酮的含量(3 816.6mg/g DW)和生产量(18 654.0mg/L)在光照强度30("mol·cm~(-2)·s~(-1))时达到最大值。因此,光照强度是狼爪瓦松愈伤组织有效物质积累和生物量增加的影响因素之一,在愈伤组织培养时将光照强度调节为30("mol·cm~(-2)·s~(-1))较为适宜。
In this study,the calluses induced from O.cartilagineus seeds were used as the experimental material to investigate the effects of light intensity on callus biomass and effective compound accumulation.The results showed that the light intensity from 15 to 30 "mol·cm~(-2)·s~(-1) was beneficial to callus biomass accumulation,the fresh and dry weight reached the maximum level.For effective compound accumulation,the highest total phenolic content(17.4mg/g DW)and productivity(68.53mg/L)were found under 15 "mol·cm~(-2)·s~(-1)of light intensity,but the maximum polysaccharide content(125.8 mg/g DW)and productivity(615.05mg/L)were obtained under 30 "mol·cm~(-2)·s~(-1) of light intensity.In addition,light intensity also affected flavonoid accumulation,15 "mol·cm~(-2)·s~(-1) of light intensity was the most favorable for kaempferol-3-O-rutinoside accumulation,but the quercetin,kaempferol,epicatechin gallate,and isoquercitrin enhanced under 30 "mol·cm~(-2)·s~(-1) of light intensity.The total flavonoid content(3 816.6mg/g DW)and productivity(18 654.0mg/L)also reached the maximum under 30 "mol·cm~(-2)·s~(-1) light intensity.Therefore,the light intensity is one of important factors in O.cartilaginous callus culture for production of effective compounds and 30 "mol·cm~(-2)·s~(-1) is the suitable light intensity.
In this study,the calluses induced from O.cartilagineus seeds were used as the experimental material to investigate the effects of light intensity on callus biomass and effective compound accumulation.The results showed that the light intensity from 15 to 30 "mol·cm~(-2)·s~(-1) was beneficial to callus biomass accumulation,the fresh and dry weight reached the maximum level.For effective compound accumulation,the highest total phenolic content(17.4mg/g DW)and productivity(68.53mg/L)were found under 15 "mol·cm~(-2)·s~(-1)of light intensity,but the maximum polysaccharide content(125.8 mg/g DW)and productivity(615.05mg/L)were obtained under 30 "mol·cm~(-2)·s~(-1) of light intensity.In addition,light intensity also affected flavonoid accumulation,15 "mol·cm~(-2)·s~(-1) of light intensity was the most favorable for kaempferol-3-O-rutinoside accumulation,but the quercetin,kaempferol,epicatechin gallate,and isoquercitrin enhanced under 30 "mol·cm~(-2)·s~(-1) of light intensity.The total flavonoid content(3 816.6mg/g DW)and productivity(18 654.0mg/L)also reached the maximum under 30 "mol·cm~(-2)·s~(-1) light intensity.Therefore,the light intensity is one of important factors in O.cartilaginous callus culture for production of effective compounds and 30 "mol·cm~(-2)·s~(-1) is the suitable light intensity.
引文
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[20]汪海峰,钧兴荣,何广斌,等.不同海拔高度和生长季节对银杏叶中黄酮含量的形响[J].林产化学与工业,2002,22(04):47-50.
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[22]秦公伟,曹小勇,张静,等.银杏叶片愈伤组织培养及光暗条件对黄酮含量的影响[J].陕西理工学院学报(自然科学版),2011,27(01):71-76.
[23]刘蓉,杨国伟,吴月燕,等.光照强度对葡萄愈伤组织形成过程中相关酶活性和基因表达的影响[J].生物工程学报,2015,31(08):1219-1229.
[2]郑万金,张萍,仲英.瓦松属植物的研究进展[J].齐鲁药事,2008,27(03):161-163.
[3]郑艳.安徽景天科野生资源及开发展望[J].安徽师大学报(自然科学版),1998,21(04):73-76.
[4]金英花,朴哲龙.吉林省龙井地区瓦松属植物种群生态特性[J].延边大学农学学报,2014,36(04):306-310..
[5]王亚红,王亚丽,杨艳俊.大孔树脂纯化瓦松中总黄酮的工艺[J].中国医院药学杂志,2014,34(10):791-794.
[6]丁芬,王亚茹.狼爪瓦松粗提物抑菌效果研究[J].黑龙江医药,2013,26(02):227-228.
[7]任荣,柴军红,金志民,等.狼爪瓦松全草粗提物体外抑菌作用研究[J].安徽农业科学,2011,39(14):8353-8354.
[8]乌兰格日乐,白海泉,翁惠.黄酮的抗氧化活性活性研究进展[J].内蒙古民族大学学报(自然科学版),2008,23(03):276-280.
[9]王长远,吴洪奎,于长青,等.黄酮类化合物研究进展[J].黑龙江八一农垦大学学报,2007,19(02),75-78.
[10]刘颖,李东杰,魏景芳.药用植物细胞生物反应器技术的研究进展[J].中国生物工程杂志,2005,50(18):67-70.
[11]许明子,李美善.高山红景天愈伤组织诱导及生长研究[J].延边大学农学学报,2003,25(04):1-3.
[12]邵春绘,廉美兰,李铁军,等.几种因素对高山红景天愈伤组织诱导和增殖的影响[J].延边大学农学学报,2013,35(04):289-292.
[13]Ouyang J,Wang X,Zhao B,et al.Light intensity and spectral quality influencing the callus growth ofCistanche deserticola and biosynthesis of phenylethanoid glycosides[J].Plant Science,2003,165(03):657-661.
[14]Lemmon H R,Sham J,Chau L A,et al.High molecular weight polysaccharides are key immunomodulators in North American ginseng extracts:characterization of the ginseng genetic signature in primary human immune cells[J].Journal of Ethnopharmacology,2012,142(01):1-13.
[15]Hossain M A,Shah M D,Sang S V,et al.Chemical composition and antibacterial properties of the essential oils and crude extracts of Merrrmia borneensis[J].Journal of King Saud University-Science,2012,24(07):243-249.
[16]于帅,杜彬,杨越冬,等.植物中多酚的研究进展[J].河北科技师范学院学报,2011,25(03),68-73.
[17]王华田,谢宝东,姜岳忠,等.光照强度对银杏叶片发育及黄酮和内脂含量的形响[J].江西农业大学学报,2002,24(05):618-622.
[18]Agati G,Galardi C,Gravano E,et al.Flavonoid distribution in tissues of Phillyrea latifolia L.Leaves as estimated by microspetrofluorometry and multispectral fluorescence microimaging[J].Photochemistry and Photobiology,2002,76(03):350-360.
[19]李卫东,粱慈珍,卢为国,等.大豆籽拉异黄酮含量与生态因子相关系的研究[J].中国农业科学,2004,37(10):1458-1463..
[20]汪海峰,钧兴荣,何广斌,等.不同海拔高度和生长季节对银杏叶中黄酮含量的形响[J].林产化学与工业,2002,22(04):47-50.
[21]王鹏姬,冯佰利.荞麦愈伤组织培养及其黄酮和成研究[D].杨凌:西北农林科技大学,2013.
[22]秦公伟,曹小勇,张静,等.银杏叶片愈伤组织培养及光暗条件对黄酮含量的影响[J].陕西理工学院学报(自然科学版),2011,27(01):71-76.
[23]刘蓉,杨国伟,吴月燕,等.光照强度对葡萄愈伤组织形成过程中相关酶活性和基因表达的影响[J].生物工程学报,2015,31(08):1219-1229.