狼爪瓦松提取物对伤寒杆菌和痢疾杆菌的抑菌活性机制
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摘要
研究狼爪瓦松提取物对伤寒杆菌和痢疾杆菌的抑菌效果及机理.实验结果表明:乙酸乙酯相、石油醚相和氯仿相对伤寒杆菌和痢疾杆菌有明显的抑菌效果,与对照组相比差异极显著(p<0.01),各提取物对实验菌的最小抑菌质量浓度(MIC)差异较大,乙酸乙酯和石油醚提取液对伤寒杆菌的MIC为1.5 mg/mL,乙酸乙酯提取液对痢疾杆菌的MIC为1mg/mL;各种瓦松提取液作用于伤寒杆菌和痢疾杆菌可导致菌体内有电解质渗透,与对照组相比差异显著(p<0.05),即瓦松提取液可能通过干扰细菌细胞壁的合成以及抑制生物被膜的形成而抑制伤寒杆菌和痢疾杆菌生长,其抑制作用随提取液质量浓度的提高及作用时间的延长而提高.
We studied the antibacterial effects and mechanism of extracts from Orostachys cartilaginous A.Bor on Salmonella typhoon and Shigella dysenteric.The experimental results show that there are obvious antibacterial effects of ethyl acetate,petroleum ether and chloroform phases on S.typhoon and S.dysenteric.Compared with the control group,the difference is very significant(p<0.01).The minimum inhibitory mass concentration(MIC)of the extracts on the experimental bacteria are obviously different.The MIC of ethyl acetate and petroleum ether extract on S.typhoon are 1.5 mg/mL,and the MIC of ethyl acetate on S.dysenteric is 1 mg/mL.O.cartilaginous A.Bor may inhibit growth of S.typhoonand S.dysenteric by interfering with the synthesis of cytoderm and inhibition of biofilm formation.The antibacterial effects increase with the increase of the mass concentration of the extract and the prolonging of the action time.
We studied the antibacterial effects and mechanism of extracts from Orostachys cartilaginous A.Bor on Salmonella typhoon and Shigella dysenteric.The experimental results show that there are obvious antibacterial effects of ethyl acetate,petroleum ether and chloroform phases on S.typhoon and S.dysenteric.Compared with the control group,the difference is very significant(p<0.01).The minimum inhibitory mass concentration(MIC)of the extracts on the experimental bacteria are obviously different.The MIC of ethyl acetate and petroleum ether extract on S.typhoon are 1.5 mg/mL,and the MIC of ethyl acetate on S.dysenteric is 1 mg/mL.O.cartilaginous A.Bor may inhibit growth of S.typhoonand S.dysenteric by interfering with the synthesis of cytoderm and inhibition of biofilm formation.The antibacterial effects increase with the increase of the mass concentration of the extract and the prolonging of the action time.
引文
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[14]凌寿坚,黄晨娟,李慧冰.葡萄球菌种间对红霉素和克林霉素的诱导耐药性研究[J].国际检验医学杂志,2007,28(7):583-584.(LING Shoujian,HUANG Chenjuan,LI Huibing.Study on Erythromycin and Clindamycin Resistance Induced by Different Species of Staphylococcus[J].International Journal of Laboratory Medicine,2007,28(7):583-584.)
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[3]任荣,柴军红,金志民,等.瓦松属植物化学成分及其生物活性研究进展[J].安徽农业科学,2011,39(22):13412-13414.(REN Rong,CHAI Junhong,JIN Zhimin,et al.Advance on the Research of Chemical Constituents and Bioactivities from Plants of Genus Orostachys[J].Journal of Anhui Agriculture Science,2011,39(22):13412-13414.)
[4]李国旺,苗志国,赵恒章.大黄的体外抑菌实验[J].光谱实验室,2011,28(1):66-68.(LI Guowang,MIAO Zhiguo,ZHAO Hengzhang.In vitro Antibacterial Experiments of Rhubarb[J].Chinese Journal of Spectroscopy Laboratory,2011,28(1):66-68.)
[5]李秀云,万川,杜士杰,等.二芳基肟苄醚类化合物的合成及杀菌活性[J].高等学校化学学报,2015,36(12):2415-2420.(LI Xiuyun,WAN Chuan,DU Shijie,et al.Synthesis and Fungicidal Activities of Biaryl Methanone O-Benzyl Oximes[J].Chem J Chinese Universities,2015,36(12):2415-2420.)
[6]周燚,刘暑燕,邹丽芳,等.几种野生植物提取物抑菌作用研究[J].中国野生植物作物资源,2004,23(2):30-32.(ZHOU Yi,LIU Shuyan,ZOU Lifang,et al.The Inhibiting Effect of Extracts from Some Wild Plants on Plant Pathogens[J].Chinese Wild Plant Resources,2004,23(2):30-32.)
[7]周小楠,董群.金银花等6种中草药对痢疾杆菌体外抑菌效果研究[J].安徽农业科学,2012,40(6):3278-3279.(ZHOU Xiaonan,DONG Qun.Study on the Antibacterial Effects of 6 Kinds of Chinese Herbs Medicines on Shigella dysenteriae in vitro[J].Journal of Anhui Agriculture Science,2012,40(6):3278-3279.)
[8]孟凤霞,王义冠,冯磊,等.我国登革热疫情防控与媒介伊蚊的综合治理[J].中国媒介生物学及控制杂志,2015,26(1):4-10.(MENG Fengxia,WANG Yiguan,FENG Lei,et al.Review on Dengue Prevention and Control and Integrated Mosquito Management in China[J].Chin J Vector Biology Control,2015,26(1):4-10.)
[9]孙延忠,曾洪梅,石义萍,等.武夷菌素对番茄灰霉菌(Botrytis cinerea)的作用方式[J].植物病理学报,2003,33(5):434-438.(SUN Yanzhong,ZENG Hongmei,SHI Yiping,et al.Mode of Action of Wuyiencin on Botrytis cinerea[J].Acta Phytopathologica Sinica,2003,33(5):434-438.)
[10]孙菊英,郭朝晖.十种马勃体外抑菌作用的实验研究[J].中药材,1994,17(4):37-38.(SUN Juying,GUO Chaohui.The Research of 10Puffball Bacteriostasis Experiment in vitro[J].Traditional Chinese Medicine,1994,17(4):37-38.)
[11]何丽芳,罗秀云,王芳宇.芒果多酚的微波萃取及抑菌作用[J].贵州农业科学,2012,40(11):209-212.(HE Lifang,LUO Xiuyun,WANG Fangyu.The Microwave Extraction and Antibacterial Activity of Mango Polyphenols[J].Guizhou Agricultural Sciences,2012,40(11):209-212.)
[12]McCann M C,Bush M,Milioni D.Analytical Approaches to the Neural Net Architecture Design[J].Cross Ref,2001,57(6):811-821.
[13]周磊,云宝仪,汪业菊.大黄素对金黄色葡萄球菌的抑菌作用机制[J].中国生物化学与分子生物学报,2011,27(12):1156-1160.(ZHOU Lei,YUN Baoyi,WANG Yeju.Antibacterial Mechanism of Emodin on Staphylococcus aureus[J].Chinese Journal of Biochemistry and Molecular Biology,2011,27(12):1156-1160.)
[14]凌寿坚,黄晨娟,李慧冰.葡萄球菌种间对红霉素和克林霉素的诱导耐药性研究[J].国际检验医学杂志,2007,28(7):583-584.(LING Shoujian,HUANG Chenjuan,LI Huibing.Study on Erythromycin and Clindamycin Resistance Induced by Different Species of Staphylococcus[J].International Journal of Laboratory Medicine,2007,28(7):583-584.)