狼爪瓦松、钝叶瓦松和库页红景天提取物的红外光谱分析
|
摘要
目的:研究长白山地区狼爪瓦松(Orostachys cartilaginea)、钝叶瓦松(Orostachys malacophyllus)和库页红景天(Rhodiola sachalinensis)的红外光谱鉴别。方法:分别用石油醚、乙酸乙酯、甲醇进行提取,归纳不同溶剂提取物的红外光谱特征,结合SPSS(Statistical Product and Service Solutions)软件对红外谱图对比分析。结果:狼爪瓦松、钝叶瓦松的提取物的红外光谱中存在共有峰和差异性,狼爪瓦松、钝叶瓦松甲醇提取物的红外光谱可以对狼爪瓦松、钝叶瓦松进行区分。结论:该方法快速、简便、特征性强,为景天科植物的药用植物质量评价提供依据。
Objective:Identification method for infrared spectra of Orostachys cartilaginea,Orostachys malacophyllus and Rhodiola sachalinensis was investigated. Methods:Petroleum ether,methanol and ethyl acetate was used to extract Orostachys cartilaginea,Orostachys malacophyllus and Rhodiola sachalinensis from Changbai Mountains,respectively. The FT-IR( Fourier Transform infrared spectroscopy) spectra of these extracts was determined. In the end,the pattern of FT-IR spectra was compared with SPSS( Statistical Product and Service Solutions. Results:There is difference and common for FT-IR spectra of,O. cartilaginea and O. malacophyllus,furthermore,FT-IR spectra of methanol extract for O.cartilaginea and O. malacophyllus can be distinguished the community of Orostachys plant. Conclusion:This method is rapid,simple and characteristic. The reference of the identification and selection Crassulaceae species will be provided.
Objective:Identification method for infrared spectra of Orostachys cartilaginea,Orostachys malacophyllus and Rhodiola sachalinensis was investigated. Methods:Petroleum ether,methanol and ethyl acetate was used to extract Orostachys cartilaginea,Orostachys malacophyllus and Rhodiola sachalinensis from Changbai Mountains,respectively. The FT-IR( Fourier Transform infrared spectroscopy) spectra of these extracts was determined. In the end,the pattern of FT-IR spectra was compared with SPSS( Statistical Product and Service Solutions. Results:There is difference and common for FT-IR spectra of,O. cartilaginea and O. malacophyllus,furthermore,FT-IR spectra of methanol extract for O.cartilaginea and O. malacophyllus can be distinguished the community of Orostachys plant. Conclusion:This method is rapid,simple and characteristic. The reference of the identification and selection Crassulaceae species will be provided.
引文
[1]刘晓艳,尹秀梅,王思宏,等.长白山地区长药八宝抗氧化活性的研究[J].食品科技,2017,42(1):96-100.
[2]刘晓艳.长药八宝的抗氧化活性及其有效成分的分离研究[D].延吉:延边大学,2017.
[3]李承范,王思宏,李东浩.狼爪瓦松提取物的抗氧化活性的研究[J].食品科技,2015,40(4):285-288.
[4]尹秀梅,王思宏.长白山景天科植物品种、植物成分及酶抑制活性的研究进展[J].延边大学学报(自然科学版),2015,41(1):89-94.
[5]袁鹏.钝叶瓦松中活性成分的研究[D].延吉:延边大学,2016.
[6]尹秀梅,金春梅,王思宏.长白山地区钝叶瓦松茎提取物的成分及活性研究[J].中草药,2017,48(5):859-862.
[7]张蕾,潘一鸣,宛春雷,等.狼爪瓦松提取物对伤寒杆菌和痢疾杆菌的抑菌活性机制[J].吉林大学学报:理学版,2017,55(6):1621-1625.
[8]关清文,朴炫春,孙雪,等.狼爪瓦松生物学特性研究[J].林业调查规划,2017,42(5):37-40.
[9]全炳武,金明植,李东浩.长白山东北坡野生有毒植物资源[J].延边大学农学学报,2002,24(2):130-137.
[10]王思宏,尹起范,范艳玲,等.长白山地区几种红景天品种的傅里叶变换红外光谱法鉴别研究[J].光谱学与光谱分析,2004,24(8):957-959.
[11]Chiang HM,Chen HC,Wu CS,et al. Rhodiola plants:Chemistry and biological activity[J]. Journal of Food and Drug Analysis,2015,23(3):359-369.
[12]袁琦,侯林,崔清华,等.红景天甲醇冷浸提取物的体外抗病毒谱及抗病毒机制的研究[J].中华中医药学刊,2017,35(9):2345-2348.
[13]曾晓璇.鄂西北地区4种柴胡属植物分子鉴别及柴胡药材活性成分近红外定量方法研究[D].武汉:湖北中医药大学,2017.
[14]Wang P,Zhou HL,Xue FZ,et al. Analysis of infrared spectra of 60 kinds of plant extract of traditional Chinese medicine and study on the identification and evaluation of characteristics of the regional markers associated with cold and heat nature[J].Spectroscopy&Spectral Analysis,2014,34(1):58-63.
[15]Chen J,Guo B,Yan R,et al. Rapid and automatic chemical identification of the medicinal flower buds of Lonicera plants by the benchtop and hand-held Fourier transform infrared spectroscopy[J]. Spectrochimica Acta Part A Molecular&Biomolecular Spectroscopy,2017,182:81-86.
[16]王夏,孙素琴,徐荣,等.红外光谱结合聚类分析法快速鉴别不同加工方式肉苁蓉[J].中国现代中药,2017,19(5):657-661.
[17]Qu L,Chen JB,Zhang GJ,et al. Chemical profiling and adulteration screening of Aquilariae Lignum Resinatum by Fourier transform infrared(FT-IR)spectroscopy and two-dimensional correlation infrared(2D-IR)spectroscopy[J]. Spectrochimica Acta Part A Molecular&Biomolecular Spectroscopy,2017,174:177-182.
[18]姜泽明,周甜甜,卜洪洋,等.落叶松树皮原花青素生产过程的红外光谱分析[J].光谱学与光谱分析,2018,38(1):62-67.
[19]李阳,赵晨翔,张雅敏.中药提取物抗肿瘤作用及其机制的研究进展[J].中华中医药学刊,2017,35(11):2787-2790.
[20]孙素琴,周群,陈建波.中药红外光谱分析与鉴定[M].北京:化学工业出版社,2010.
[21]王璐瑶,万定荣,陈雨洁.景天属几种药用植物及混淆品的叶表皮比较鉴定[J].中华中医药杂志,2012,27(10):2672-2675.
[22]Xu R,Chen YJ,Wan DR,et al. Identification of four Sedum plant medicines by fourier transform infrared spectra[J]. Pharmacognosy Magazine,2012,8(30):107-110.
[2]刘晓艳.长药八宝的抗氧化活性及其有效成分的分离研究[D].延吉:延边大学,2017.
[3]李承范,王思宏,李东浩.狼爪瓦松提取物的抗氧化活性的研究[J].食品科技,2015,40(4):285-288.
[4]尹秀梅,王思宏.长白山景天科植物品种、植物成分及酶抑制活性的研究进展[J].延边大学学报(自然科学版),2015,41(1):89-94.
[5]袁鹏.钝叶瓦松中活性成分的研究[D].延吉:延边大学,2016.
[6]尹秀梅,金春梅,王思宏.长白山地区钝叶瓦松茎提取物的成分及活性研究[J].中草药,2017,48(5):859-862.
[7]张蕾,潘一鸣,宛春雷,等.狼爪瓦松提取物对伤寒杆菌和痢疾杆菌的抑菌活性机制[J].吉林大学学报:理学版,2017,55(6):1621-1625.
[8]关清文,朴炫春,孙雪,等.狼爪瓦松生物学特性研究[J].林业调查规划,2017,42(5):37-40.
[9]全炳武,金明植,李东浩.长白山东北坡野生有毒植物资源[J].延边大学农学学报,2002,24(2):130-137.
[10]王思宏,尹起范,范艳玲,等.长白山地区几种红景天品种的傅里叶变换红外光谱法鉴别研究[J].光谱学与光谱分析,2004,24(8):957-959.
[11]Chiang HM,Chen HC,Wu CS,et al. Rhodiola plants:Chemistry and biological activity[J]. Journal of Food and Drug Analysis,2015,23(3):359-369.
[12]袁琦,侯林,崔清华,等.红景天甲醇冷浸提取物的体外抗病毒谱及抗病毒机制的研究[J].中华中医药学刊,2017,35(9):2345-2348.
[13]曾晓璇.鄂西北地区4种柴胡属植物分子鉴别及柴胡药材活性成分近红外定量方法研究[D].武汉:湖北中医药大学,2017.
[14]Wang P,Zhou HL,Xue FZ,et al. Analysis of infrared spectra of 60 kinds of plant extract of traditional Chinese medicine and study on the identification and evaluation of characteristics of the regional markers associated with cold and heat nature[J].Spectroscopy&Spectral Analysis,2014,34(1):58-63.
[15]Chen J,Guo B,Yan R,et al. Rapid and automatic chemical identification of the medicinal flower buds of Lonicera plants by the benchtop and hand-held Fourier transform infrared spectroscopy[J]. Spectrochimica Acta Part A Molecular&Biomolecular Spectroscopy,2017,182:81-86.
[16]王夏,孙素琴,徐荣,等.红外光谱结合聚类分析法快速鉴别不同加工方式肉苁蓉[J].中国现代中药,2017,19(5):657-661.
[17]Qu L,Chen JB,Zhang GJ,et al. Chemical profiling and adulteration screening of Aquilariae Lignum Resinatum by Fourier transform infrared(FT-IR)spectroscopy and two-dimensional correlation infrared(2D-IR)spectroscopy[J]. Spectrochimica Acta Part A Molecular&Biomolecular Spectroscopy,2017,174:177-182.
[18]姜泽明,周甜甜,卜洪洋,等.落叶松树皮原花青素生产过程的红外光谱分析[J].光谱学与光谱分析,2018,38(1):62-67.
[19]李阳,赵晨翔,张雅敏.中药提取物抗肿瘤作用及其机制的研究进展[J].中华中医药学刊,2017,35(11):2787-2790.
[20]孙素琴,周群,陈建波.中药红外光谱分析与鉴定[M].北京:化学工业出版社,2010.
[21]王璐瑶,万定荣,陈雨洁.景天属几种药用植物及混淆品的叶表皮比较鉴定[J].中华中医药杂志,2012,27(10):2672-2675.
[22]Xu R,Chen YJ,Wan DR,et al. Identification of four Sedum plant medicines by fourier transform infrared spectra[J]. Pharmacognosy Magazine,2012,8(30):107-110.