狼爪瓦松提取物的抗氧化活性的研究
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摘要
为研究狼爪瓦松的药用价值,通过DPPH自由基清除能力,羟自由基清除能力和超氧阴离子清除能力来评价其抗氧化活性。实验结果显示狼爪瓦松乙醇提取物对DPPH自由基的清除率为99.15%,羟基自由基清除率为94.55%,超氧阴离子清除率为61.33%。狼爪瓦松中黄酮类化合物对清除自由基有一定的作用,其清除效果在一定范围内随着总黄酮质量浓度的增加而增强。
To investigate the medicinal value of Orostachys cartilaginous,the antioxidant activity is evaluated by 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical,hydroxyl radical and superoxide anion radical scavenging ability of DPPH.The results revealed that DPPH radical scavenging ability of is 99.15%,hydroxyl adical scavenging rability is 94.55% and superoxide anion radical scavenging ability is 61.33% of Orostachys cartilaginous ethanol extract.The results of antioxidant activity shows that the total flavonoids of Orostachys cartilaginous extracts implied their definite radical scavenging ablity,in addition,within certain concentration the scavenging effect is enhanced with increasing concentration of total flavonoids.
To investigate the medicinal value of Orostachys cartilaginous,the antioxidant activity is evaluated by 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical,hydroxyl radical and superoxide anion radical scavenging ability of DPPH.The results revealed that DPPH radical scavenging ability of is 99.15%,hydroxyl adical scavenging rability is 94.55% and superoxide anion radical scavenging ability is 61.33% of Orostachys cartilaginous ethanol extract.The results of antioxidant activity shows that the total flavonoids of Orostachys cartilaginous extracts implied their definite radical scavenging ablity,in addition,within certain concentration the scavenging effect is enhanced with increasing concentration of total flavonoids.
引文
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[12]郭豫梅.鸡油菌中总黄酮含量的测定及抗氧化活性研究[J].食品研究与开发,2014,35(7):96-9
[2]郑万金,张萍,仲英.瓦松属植物的研究进展[J].齐鲁药事,2008,27(3):161-163
[3]郑艳.安徽景天科野生资源及开发展望[J].安徽师大学报(自然科学版),1998,21(4):73-76
[4]周繇.中国长白山植物资源志[M].中国林业出版社,2010
[5]祝廷成,严仲铠,周守标.中国长白山植物[M].北京:科学技术出版社,2003
[6]王亚红,王亚丽,杨艳俊.大孔树脂纯化瓦松中总黄酮的工艺[J].中国医院药学杂志,2014,34(10):791-794
[7]丁芬,王亚茹.狼爪瓦松粗提物抑菌效果研究[J].黑龙江医药,2013,26(2):227-228
[8]任荣,柴军红,金志民,等.狼爪瓦松全草粗提物体外抑菌作用研究[J].安徽农业科学,2011,39(14):8353-8354,8356
[9]任荣,柴军红,金志民,等.瓦松属植物化学成分及其生物活性研究进展[J].安徽农业科学,2011,39(22):13412-13414,13416
[10]耿敬章,冯君琪.黄酮类化合物的生理功能与应用研究[J].中国食物与营养,2007,(7):19-21
[11]李利华.香菇中总黄酮含量的测定及抗氧化活性研究[J].光谱实验室,2009,26(3):680-684
[12]郭豫梅.鸡油菌中总黄酮含量的测定及抗氧化活性研究[J].食品研究与开发,2014,35(7):96-9