狼爪瓦松中活性成分提取工艺的优化
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摘要
为建立狼爪瓦松叶中黄酮、萜类同步快速提取工艺,为狼爪瓦松活性成分开发提供理论基础,以狼爪瓦松为原料,以总黄酮、总萜提取率为技术指标,通过提取方法对比试验,采用正交设计探讨提取工艺的影响因素。结果表明:各因素影响依次为提取时间>温度>功率>固液比,其最佳工艺参数为超声温度60℃,固液比1∶20,提取时间40min,超声波功率100W,该工艺下,2种活性物质主要指标均比传统提取法高,总萜得率为2.66%,总黄酮得率为0.901%。放大10倍试验表明,工艺较稳定,标准偏差均在2.5%以内。
To optimize the extraction process of flavonoids,terpene from O.cartilaginous,which is expected to provide theoretical basis for extracting active components from O.cartilaginous.O.cartilaginous was used as raw material,the extraction rate of total flavonoids and total terpene is technical index,a comparative test on extraction methods,the effect of the extraction process was optimized by the orthogonal experimental design.Results:The factors was as follows ultrasonic time>temperature> power>solid-liquid ratio.The optimum technological parameters were ultrasonic time40 min,temperature 60℃,solid-liquid ratio 1∶20,power 100 W.Extraction yield of terpene was 2.66%,and flavonoids was 0.901%.The 10-times amplification test showed that the process was more stable;standard deviations were less than 2.5%.
To optimize the extraction process of flavonoids,terpene from O.cartilaginous,which is expected to provide theoretical basis for extracting active components from O.cartilaginous.O.cartilaginous was used as raw material,the extraction rate of total flavonoids and total terpene is technical index,a comparative test on extraction methods,the effect of the extraction process was optimized by the orthogonal experimental design.Results:The factors was as follows ultrasonic time>temperature> power>solid-liquid ratio.The optimum technological parameters were ultrasonic time40 min,temperature 60℃,solid-liquid ratio 1∶20,power 100 W.Extraction yield of terpene was 2.66%,and flavonoids was 0.901%.The 10-times amplification test showed that the process was more stable;standard deviations were less than 2.5%.
引文
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[8]高茗,胡玉霞,余启荣,等.UV-Vis法测定灵丹草油中总萜类含量[J].现代中药研究与实践,2011,25(1):60-62.
[2]郑艳,巩劼,陈士超,等.瓦松(Orostachys fimbriatus)的形态解剖学研究[J].植物研究,2003,23(2):164.
[3]郑艳徐,珞珊.中国瓦松属(Orostachys)的药用资源[J].中国中医药信息杂志,2003,10(11):41-42.
[4]赵艳杰,刘晓娟.瓦松中强心成分的提取和药理作用的初步研究[J].锦州医学院学报,1992,13(4):13.
[5]马怀良,龚振杰,柴军红,等.超声波浸提黑木耳菌糠纤维素酶、木聚糖酶条件及其部分酶学性质研究[J].东北农业大学学报,2012,43(5):117-121.
[6]旷春桃,李湘洲,汪玉霞,等.大叶冬青叶中总黄酮测定方法和提取工艺研究[J].食品科学,2009,30(6):49-51.
[7]王翔飞,潘旭东,刘雯霞,等.黄花瓦松中总三萜成分的含量的测定[J].中国民族民间医药,2009,10(3):11.
[8]高茗,胡玉霞,余启荣,等.UV-Vis法测定灵丹草油中总萜类含量[J].现代中药研究与实践,2011,25(1):60-62.