猪毛蒿不同炮制方法炮制前后化学成分变化
|
摘要
[目的]研究猪毛蒿不同炮制方法炮制前后水提取物、醇提取物含量、挥发性成分以及绿原酸含量差异,为寻找猪毛蒿炮制方法奠定基础.[方法]以重量法测定水提取物、醇提取物含量,GC-MS/MS测定挥发性成分,HPLC-UV测定绿原酸含量.[结果]与生品相比,晒干品的水、醇提取物含量均显著下降;烘干品水提取物含量下降,而醇提取物含量提高;阴干品无显著差异.GC-MS/MS分析不同炮制品中挥发性成分,从生品、阴干、晒干、烘干品中分别检出25、21、27、24个色谱峰,共有的组分有13个,且猪毛蒿炮制前后既有含量的改变,也有不同结构的成分出现,晒干与生品差距最大;HPLC-UV方法测定了绿原酸含量,发现炮制后均显著下降,含量从大到小依次为生品、阴干、晒干、烘干.[结论]猪毛蒿在加工过程中应尽量采用阴干方法,以防止有效成分损失.
[Objective] The research aimed to study the difference of water extract, alcohol extract content, volatile component and chlorogenic acid content before and after the processing of Artemisia scoparia in different processing methods, which laid the foundation for searching the method of processing Artemisia scoparia. [Method]The gravimetric method was used to determine the water and alcohol extract content. GCMS/MS method was applied to determine the volatile component and HPLC-UV assay was used to determine the chlorogenic acid content. [Result]Compared with the crude materials, the water and alcohol extracts in the products dried in the sun decreased significantly; the content of water extract of dried product decreased, but the content of alcohol extract increased; there was no significant difference between crude and dried in the shade. GC-MS/MS analysis of volatile components in different processed products,25, 21, 27, and 24 peaks were respectively detected in crude, dried in the shade, in the sun and in the drying oven, 13 mutual peaks were found in the four materials,and Artemisia scoparia before and after processing both the content of the change, there were also different components of the structure, the difference between dried in the sun and crude was the biggest. Content of chlorogenic acid determined by HPLC-UV method,that found the content was decreased significantly after processing,the content in the order below: crude > dried in the shade > dried in the sun > dried in the drying oven. [Conclusion] Artemisia scoparia shall be dried in the shade during the process in order to avoid the loss of the effective components.
[Objective] The research aimed to study the difference of water extract, alcohol extract content, volatile component and chlorogenic acid content before and after the processing of Artemisia scoparia in different processing methods, which laid the foundation for searching the method of processing Artemisia scoparia. [Method]The gravimetric method was used to determine the water and alcohol extract content. GCMS/MS method was applied to determine the volatile component and HPLC-UV assay was used to determine the chlorogenic acid content. [Result]Compared with the crude materials, the water and alcohol extracts in the products dried in the sun decreased significantly; the content of water extract of dried product decreased, but the content of alcohol extract increased; there was no significant difference between crude and dried in the shade. GC-MS/MS analysis of volatile components in different processed products,25, 21, 27, and 24 peaks were respectively detected in crude, dried in the shade, in the sun and in the drying oven, 13 mutual peaks were found in the four materials,and Artemisia scoparia before and after processing both the content of the change, there were also different components of the structure, the difference between dried in the sun and crude was the biggest. Content of chlorogenic acid determined by HPLC-UV method,that found the content was decreased significantly after processing,the content in the order below: crude > dried in the shade > dried in the sun > dried in the drying oven. [Conclusion] Artemisia scoparia shall be dried in the shade during the process in order to avoid the loss of the effective components.
引文
[1]国家药典委员会.中华人民共和国药典:2015版一部[S].北京:中国医药科技出版社,2015:239-240.
[2]刘洪玲,董岩.艾蒿和猪毛蒿挥发油化学成分对比研究[J].时珍国医国药,2007,18(4):832-833.
[3]郑维发,谭仁祥,刘志礼,等.八种蒿属植物石油醚提取物中萜类成分分析[J].南京大学学报,1996,32(4):706-712.
[4]董岩,刘洪玲,王新芳.山东猪毛蒿挥发油化学成分的气相色谱-质谱研究[J].中成药,2006,28(11):1641-1644.
[5]南京中医药大学.中药大辞典:下册[M].2版.上海:上海科学技术出版社,2006.
[6]张鞍灵,马琼,高锦明,等.绿原酸及其类似物与生物活性[J].中草药,2001,32(2):173-176.
[7]高锦明,张鞍灵,赵晓明,等.绿原酸分布、提取与生物活性研究综述[J].西北林学院学报,1999,14(2):73-82.
[8]尼玛次仁,格桑顿珠,格桑次仁,等.HPLC法测定藏药猪毛蒿中绿原酸的含量[J].中国民族医药杂志,2009(4):51-52.
[2]刘洪玲,董岩.艾蒿和猪毛蒿挥发油化学成分对比研究[J].时珍国医国药,2007,18(4):832-833.
[3]郑维发,谭仁祥,刘志礼,等.八种蒿属植物石油醚提取物中萜类成分分析[J].南京大学学报,1996,32(4):706-712.
[4]董岩,刘洪玲,王新芳.山东猪毛蒿挥发油化学成分的气相色谱-质谱研究[J].中成药,2006,28(11):1641-1644.
[5]南京中医药大学.中药大辞典:下册[M].2版.上海:上海科学技术出版社,2006.
[6]张鞍灵,马琼,高锦明,等.绿原酸及其类似物与生物活性[J].中草药,2001,32(2):173-176.
[7]高锦明,张鞍灵,赵晓明,等.绿原酸分布、提取与生物活性研究综述[J].西北林学院学报,1999,14(2):73-82.
[8]尼玛次仁,格桑顿珠,格桑次仁,等.HPLC法测定藏药猪毛蒿中绿原酸的含量[J].中国民族医药杂志,2009(4):51-52.