龙胆中当药苷和獐牙菜苦苷的含量测定及指纹图谱研究
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摘要
目的建立同时测定吉林省不同产地、不同药用部位龙胆中当药苷和獐牙菜苦苷含量的HPLC分析方法及龙胆药材的指纹图谱。研究龙胆不同产地、不同药用部位成分差异并加以区别。方法采用高效液相色谱法测定龙胆中当药苷和獐牙菜苦苷的含量,同时采用HPLC梯度洗脱技术建立指纹图谱系统,并进行聚类分析。结果当药苷和獐牙菜苦苷分别在0.1~0.35μg(r=0.999 9)、0.19~0.68μg(r=0.999 9)内呈良好的线性关系,平均加样回收率为97.40%、96.63%,RSD值分别为1.31%、1.21%。左家龙胆根中当药苷及獐牙菜苦苷的含量较高。结论龙胆中獐牙菜苦苷的含量较高,而当药苷的含量稍低。本研究将为后续龙胆的应用与开发提供理论基础。
Objective To establish the HPLC method for the simultaneous determination of chiratin and swertiamarin gentian from different areas of Jilin Province and to establish the fi ngerprints of radix gentiana. Study of different origin, different parts of the gentian medicinal differences in composition and to be distinguished.Methods HPLC were used to determine the content of chiratin and swertiamarin in Gentian. At the same time, the fi ngerprinting system was established by HPLC gradient elution technique and clustering analysis was carried out.Results The results showed that there was a good linear relationship between the chiratin and swertiamar in the range of 0.1 ～ 0.35μg(r = 0.9999) and 0.19 ～ 0.68 μg(r = 0.9999) respectively. The average recoveries were 97.40%and 96.63%, RSD values were 1.31% and 1.21% respectively. Left home gentian root contain lots of chiratin and swertiamar. Conclusion The contents of swertiamarin in gentian are higher, while the content of chiratin is lower. This study will provide the theoretical basis for the application and development of gentian.
Objective To establish the HPLC method for the simultaneous determination of chiratin and swertiamarin gentian from different areas of Jilin Province and to establish the fi ngerprints of radix gentiana. Study of different origin, different parts of the gentian medicinal differences in composition and to be distinguished.Methods HPLC were used to determine the content of chiratin and swertiamarin in Gentian. At the same time, the fi ngerprinting system was established by HPLC gradient elution technique and clustering analysis was carried out.Results The results showed that there was a good linear relationship between the chiratin and swertiamar in the range of 0.1 ～ 0.35μg(r = 0.9999) and 0.19 ～ 0.68 μg(r = 0.9999) respectively. The average recoveries were 97.40%and 96.63%, RSD values were 1.31% and 1.21% respectively. Left home gentian root contain lots of chiratin and swertiamar. Conclusion The contents of swertiamarin in gentian are higher, while the content of chiratin is lower. This study will provide the theoretical basis for the application and development of gentian.
引文
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[13]张楠,姜雨昕,任悦,等.龙胆及朝鲜龙胆中龙胆多糖与龙胆苦苷的含量测定[J].时珍国医国药, 2016,27(10):2395-2397.
[14]孙紫薇,张楠,翁丽丽,等.朝鲜龙胆的生药学研究[J].长春中医药大学学报, 2017, 33(5):720-722.
[15]陈千良,孙文基.裂环烯醚萜类化合物研究进展[J].国外医药(植物药分册), 2003, 18(2):58-63.
[16]魏岚,陈晓辉,王晓辉,等.不同产地龙胆中龙胆苦苷的含量测定[J].沈阳药科大学学报, 2004, 21(2):114-117.
[17]吴立宏.中药龙胆的应用传承[J].世界科学技术-中医药现代化, 2011, 13(2):340-347.
[18]沈涛,金航,王元忠,等.中药龙胆化学成分研究进展[J].安徽农业科学, 2010, 38(30):16868-16870, 16874.
[19]刘文杰,孙志蓉,杜远,等.不同产地铁皮石斛主要化学成分及指纹图谱研究[J].北京中医药大学学报, 2013,36(2):117-120.
[20]付娟,张海弢,王伟,等.芪白平肺颗粒HPLC指纹图谱研究及其多指标成分定量分析[J].中草药, 2017, 48(5):918-923.
[2]冯波.龙胆抗肝损伤活性成分的分离,纯化及药代动力学研究[D].长春:吉林大学, 2014.
[3]郭建华,田成旺,刘晓,等.中药环烯醚萜类化合物研究进展[J].药物评价研究, 2011, 34(4):293-297.
[4]董丽萍,倪梁红,赵志礼,等.龙胆属环烯醚萜类化学成分研究进展[J].中草药, 2017, 48(10):2116-2128.
[5]张敬莹.糙龙胆地上部分的化学成分研究[D].大连:大连理工大学, 2008.
[6] YAMADA H, KIKUCHI S, INUI T, et al. Gentiolac-tone, a Secoiridoid Dilactone from Gentiana triflora, InhibitsTNF-α,i NOS and Cox-2 m RNA Expression and Blocks NF-κBPromoterActivityinMurineMacrophages[J].PLOS One, 2014, 9(11):e113834.
[7]KESAVANR,POTUNURUUR,NASTASIJEVIB.T A,joksi G,dixit M. inhibition of vascular SmoothMuscle cell proliferation by gen-tiana lutea root extracts[J]. PLOS One,2013, 8(4):e61393.
[8]杨德义.“龙胆清胃汤”治疗糜烂性胃炎128例[J].江苏中医药, 2012, 40(2):25.
[9]霍则军,刘青云.龙胆泻肝汤加减治疗带状疱疹伴中、重度抑郁情绪[J].中国实验方剂学杂志, 2013, 19(4):300-302.
[10]郭海凤,朴惠顺.关龙胆抗肿瘤活性成分的研究[J].华西药学杂志, 2011, 26(3):204-207.
[11]ROJAS A, BAH M, ROJAS J, et al. Smooth muscle relaxing activity of gentiopicroside isolated from Gentiana[J]. Planta Medica, 2000, 66(8):765.
[12]孙紫薇,陈丽,辛宇,等.酒炙龙胆炮制工艺研究[J].人参研究, 2017, 29(6):48-50.
[13]张楠,姜雨昕,任悦,等.龙胆及朝鲜龙胆中龙胆多糖与龙胆苦苷的含量测定[J].时珍国医国药, 2016,27(10):2395-2397.
[14]孙紫薇,张楠,翁丽丽,等.朝鲜龙胆的生药学研究[J].长春中医药大学学报, 2017, 33(5):720-722.
[15]陈千良,孙文基.裂环烯醚萜类化合物研究进展[J].国外医药(植物药分册), 2003, 18(2):58-63.
[16]魏岚,陈晓辉,王晓辉,等.不同产地龙胆中龙胆苦苷的含量测定[J].沈阳药科大学学报, 2004, 21(2):114-117.
[17]吴立宏.中药龙胆的应用传承[J].世界科学技术-中医药现代化, 2011, 13(2):340-347.
[18]沈涛,金航,王元忠,等.中药龙胆化学成分研究进展[J].安徽农业科学, 2010, 38(30):16868-16870, 16874.
[19]刘文杰,孙志蓉,杜远,等.不同产地铁皮石斛主要化学成分及指纹图谱研究[J].北京中医药大学学报, 2013,36(2):117-120.
[20]付娟,张海弢,王伟,等.芪白平肺颗粒HPLC指纹图谱研究及其多指标成分定量分析[J].中草药, 2017, 48(5):918-923.