Ezrin蛋白生物学功能新进展
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摘要
Ezrin蛋白是ERM(ezrin、radixin、moesin)蛋白家族中的一员,是细胞膜和肌动蛋白细胞骨架之间重要的连接蛋白。在维持细胞形态、参与细胞运动、黏附、信号转导、调节凋亡、影响恶性肿瘤转移及预后等诸多方面均发挥重要作用。近年来,研究者发现Ezrin蛋白在调节胃酸分泌、参与内皮细胞的增殖及新生血管的形成、调节水平衡、维持内皮细胞孔径等很多方面均发挥作用。本文就Ezrin蛋白的生物学功能进行综述。
Ezrin protein is a member of the ERM(ezrin, radixin, moesin) protein family and is an important connectin between the cell membrane and the actin cytoskeleton. It plays an important role in maintaining cell morphology, participating in cell movement, adhesion, signal transduction, regulating apoptosis, affecting malignant tumor metastasis and prognosis. In recent years, researchers have found that Ezrin protein plays a role in regulating gastric acid secretion, participating in the proliferation of endothelial cells and the formation of new blood vessels, regulating water balance, and maintaining the aperture of endothelial cells. In this paper, the biological function of Ezrin protein is reviewed.
Ezrin protein is a member of the ERM(ezrin, radixin, moesin) protein family and is an important connectin between the cell membrane and the actin cytoskeleton. It plays an important role in maintaining cell morphology, participating in cell movement, adhesion, signal transduction, regulating apoptosis, affecting malignant tumor metastasis and prognosis. In recent years, researchers have found that Ezrin protein plays a role in regulating gastric acid secretion, participating in the proliferation of endothelial cells and the formation of new blood vessels, regulating water balance, and maintaining the aperture of endothelial cells. In this paper, the biological function of Ezrin protein is reviewed.
引文
[1]Hunter T,Cooper J A.Epidermal growth factor induces rapid tyrosine phosphorylation of proteins in the A431 human tumor cel[J].Cel,1981,24,741-752.
[2]Bretscher A.Purification of an 80,000-dalton protein that is a component of the isolated microvillus cytoskeleton,and its localization in nonmuscle cells[J].Cell Biol,1983,97,425-432.
[3]Martin TA,Harrison G,Mansel RE,et al.The role of the CD44/Ezrin complex in cancer metastasis[J].Crit Rev Oncol Hematol,2003,46(2):165-18.
[4]Polesello C,Payre F.Small is beautiful:what flies tell us about ERM protein function in development[J].Trends Cell Biol,2004,14(6):294-302.
[5]Fiever BT,Gautreau A,Roy C,et al.Phosphoinesitide binding and phosphorylation act sequentially in the activation mechanism of Ezrin[J].Cell bid,2004,164(5):653.
[6]肖贤军,谌南武.Ezrin蛋白与肿瘤的关系[J].实用医学杂志,2009,25(6):1002.
[7]Bonilha VL,Rayborn ME,Saotome J,et al.Microvilli defects in retinas of ezrin knockout mice[J].Exp Eye Res,2006,82(4):720-729.
[8]Pujuguet P,Del Maestrol,Gautreau A,et al.Ezrin regulates E-cadherindependent adherens junction assembly through Rac1-activation[J].Mol Biol Cell,2003,14(5):2181-2191.
[9]Strasser A,Jost PJ,Nagata S.The many roles of FAS receptor signaling in the immune system[J].Immunity,2009,30(2):180-192.
[10]Chakrabanchu K,Herincs Z,Huault S,et al.Palmitoylation is required for efficient Fas cell death signaling[J].ENBO J,2007,26(1):209-220.
[11]Kuo WC,Yang KT,Hsieh,SL,et al.Ezrin is a negative regulator of death receptor-induced apoptosis[J].Oncogene,2010,29(9):1374-1383.
[12]Khanna C,Wan X,Bose S,et al.The membrane-cytoskeleton linker Ezrin is necessary for osteosarcoma metastasis[J].Nat Med,2004,10(2):182-186.
[13]Zhang Y,Zhang L,Zhang G,et al.Osteosarcoma metastasis:prospective role of ezrin[J].Tumor Biol,2014,35(6):5055-5059.
[14]Pujuguet P,Del Maestro L,Gautreau A,et al.Ezrin regulates E-cadherindependent adherens junction assembly through Racl activation[J].Mol Biol Cell,2003,14(5):2181-2191.
[15]Encb K,Koncb S,Shackleford J,et al.Phosphorylated ezrin is associated with EBV latent membrane protein 1 in nasopharyngeal carcinoma and induces cell migration[J].Oncogene,2009,28(14):1725-1735.
[16]Louvet-Vallee S,ERM proteins:Form cellular architecture to cell signaling[J].Biol Cell,2000,92(5):305-316.
[17]Hiscox S,Jiang W G.Ezrin regulates cell-cell and cell matrix adhesion,a possible role with E-cadherin/β-catenin[J].Jonurnal of Cell Science,1999,112,3081-3090.
[18]Yu Y,Khanna C,et al.Expression profiling identifies the cytoskeletal organizer Ezrin and the developmental homoprotein Six-1 as key metastasis regulators[J].Nat Med,2004,10(2):175-181.
[19]Lugini L,Lozupone F,Matarrese P,et al.Potent phagocytic activity discriminate matastatic and primary human malignant melanoma:a key role of ezrin[J].Lab Invest,2003,83(11):1555-1567.
[20]Juliane H,santos K,Goff S P,et al.The Ezrin-Radixin-Moesin family member ezrin regulates stable microtubule formation and retroviral infection[J].J Virol,2008,82(9):4665-4670.
[21]Hunter KW.Ezrin,a key component in tumor metastasis[J].Treads Mol Med,2004,10(5):201-204.
[22]Lozupone F,Lugini L,matarrese P,et al.Identification and relevance of the CD95-binding domain in the N-terminal region of ezrin[J].J Biol Chem,2004,279(10):9919-9207.
[23]Kishore R,Qin G,Luedemann C,et al.The cytoskeletal prtein ezrin regulates EC proliferation and angiogenesis via TNF-αinduced transcriptional repression of cyclin A[J].J Clin Invest,2005,115(7):1785-1796.
[24]Liang-ping Zhao,Lei Huang,Xun Tian,et al.Knockdown of Ezrin Supresses the Migration and Angiogenesis of Human Umbilical Vein Endothelial Cells In Vitro[J].Huazhong Univ Sci Technol med sci,2016,36(2):243-248.
[25]Yao X,Thibodeau A,and Forte JG.Ezrin-calpainⅠinteractions in gastric parietal cel[J].Am J Physiol,1993,265:C36-46.
[26]Urushidani T,Muto Y,Nagao Y,et al.ME-3407,a new antiulcer agent,inhibits acid secretion by interfering with redistribution of H+-K+ATPase[J].Am J Physiol,1997,272:G1122-34.
[27]王峰松.Ezrin-ACAP4蛋白复合物在胞吐动力学调控中的功能研究[J].中国科学技术大学,2008.
[28]Caroline Stefani,David Gonzalez-Rodriguez,Yosuke Senju,et al.Ezrin enhances line tension along transcellular tunnel edges via NMⅡa driven actomyosin cable formation[J].NAT COMMUN,2017.
[29]谢帆,刘雪丽,肖卓妮,等.埃兹蛋白在延迟着床小鼠子宫内的表达及雌孕激素对其的调节作用[J].武汉大学学报(医学版).2015,36(5):702-705+723.
[30]黄伟,谢青贞,吴莲芝,等.埃兹蛋白在早孕自然流产患者蜕膜、绒毛中的表达[J]生殖医学杂志,2013,22(12):941-944.
[31]Quan C,Yan Y,Qin Z,et al.Ezrin regulates skin fibroblast size/mechanical properties and YAP-dependent proliferation[J].J cell Commun Signal,2007.
[32]Wei Li,William W.Jin,Kenji Tsuji,et al.Ezrin directly interacts with AQP2and promotes its endocytosis[J].J Cell Sci,2017,130:2914-2925.
[2]Bretscher A.Purification of an 80,000-dalton protein that is a component of the isolated microvillus cytoskeleton,and its localization in nonmuscle cells[J].Cell Biol,1983,97,425-432.
[3]Martin TA,Harrison G,Mansel RE,et al.The role of the CD44/Ezrin complex in cancer metastasis[J].Crit Rev Oncol Hematol,2003,46(2):165-18.
[4]Polesello C,Payre F.Small is beautiful:what flies tell us about ERM protein function in development[J].Trends Cell Biol,2004,14(6):294-302.
[5]Fiever BT,Gautreau A,Roy C,et al.Phosphoinesitide binding and phosphorylation act sequentially in the activation mechanism of Ezrin[J].Cell bid,2004,164(5):653.
[6]肖贤军,谌南武.Ezrin蛋白与肿瘤的关系[J].实用医学杂志,2009,25(6):1002.
[7]Bonilha VL,Rayborn ME,Saotome J,et al.Microvilli defects in retinas of ezrin knockout mice[J].Exp Eye Res,2006,82(4):720-729.
[8]Pujuguet P,Del Maestrol,Gautreau A,et al.Ezrin regulates E-cadherindependent adherens junction assembly through Rac1-activation[J].Mol Biol Cell,2003,14(5):2181-2191.
[9]Strasser A,Jost PJ,Nagata S.The many roles of FAS receptor signaling in the immune system[J].Immunity,2009,30(2):180-192.
[10]Chakrabanchu K,Herincs Z,Huault S,et al.Palmitoylation is required for efficient Fas cell death signaling[J].ENBO J,2007,26(1):209-220.
[11]Kuo WC,Yang KT,Hsieh,SL,et al.Ezrin is a negative regulator of death receptor-induced apoptosis[J].Oncogene,2010,29(9):1374-1383.
[12]Khanna C,Wan X,Bose S,et al.The membrane-cytoskeleton linker Ezrin is necessary for osteosarcoma metastasis[J].Nat Med,2004,10(2):182-186.
[13]Zhang Y,Zhang L,Zhang G,et al.Osteosarcoma metastasis:prospective role of ezrin[J].Tumor Biol,2014,35(6):5055-5059.
[14]Pujuguet P,Del Maestro L,Gautreau A,et al.Ezrin regulates E-cadherindependent adherens junction assembly through Racl activation[J].Mol Biol Cell,2003,14(5):2181-2191.
[15]Encb K,Koncb S,Shackleford J,et al.Phosphorylated ezrin is associated with EBV latent membrane protein 1 in nasopharyngeal carcinoma and induces cell migration[J].Oncogene,2009,28(14):1725-1735.
[16]Louvet-Vallee S,ERM proteins:Form cellular architecture to cell signaling[J].Biol Cell,2000,92(5):305-316.
[17]Hiscox S,Jiang W G.Ezrin regulates cell-cell and cell matrix adhesion,a possible role with E-cadherin/β-catenin[J].Jonurnal of Cell Science,1999,112,3081-3090.
[18]Yu Y,Khanna C,et al.Expression profiling identifies the cytoskeletal organizer Ezrin and the developmental homoprotein Six-1 as key metastasis regulators[J].Nat Med,2004,10(2):175-181.
[19]Lugini L,Lozupone F,Matarrese P,et al.Potent phagocytic activity discriminate matastatic and primary human malignant melanoma:a key role of ezrin[J].Lab Invest,2003,83(11):1555-1567.
[20]Juliane H,santos K,Goff S P,et al.The Ezrin-Radixin-Moesin family member ezrin regulates stable microtubule formation and retroviral infection[J].J Virol,2008,82(9):4665-4670.
[21]Hunter KW.Ezrin,a key component in tumor metastasis[J].Treads Mol Med,2004,10(5):201-204.
[22]Lozupone F,Lugini L,matarrese P,et al.Identification and relevance of the CD95-binding domain in the N-terminal region of ezrin[J].J Biol Chem,2004,279(10):9919-9207.
[23]Kishore R,Qin G,Luedemann C,et al.The cytoskeletal prtein ezrin regulates EC proliferation and angiogenesis via TNF-αinduced transcriptional repression of cyclin A[J].J Clin Invest,2005,115(7):1785-1796.
[24]Liang-ping Zhao,Lei Huang,Xun Tian,et al.Knockdown of Ezrin Supresses the Migration and Angiogenesis of Human Umbilical Vein Endothelial Cells In Vitro[J].Huazhong Univ Sci Technol med sci,2016,36(2):243-248.
[25]Yao X,Thibodeau A,and Forte JG.Ezrin-calpainⅠinteractions in gastric parietal cel[J].Am J Physiol,1993,265:C36-46.
[26]Urushidani T,Muto Y,Nagao Y,et al.ME-3407,a new antiulcer agent,inhibits acid secretion by interfering with redistribution of H+-K+ATPase[J].Am J Physiol,1997,272:G1122-34.
[27]王峰松.Ezrin-ACAP4蛋白复合物在胞吐动力学调控中的功能研究[J].中国科学技术大学,2008.
[28]Caroline Stefani,David Gonzalez-Rodriguez,Yosuke Senju,et al.Ezrin enhances line tension along transcellular tunnel edges via NMⅡa driven actomyosin cable formation[J].NAT COMMUN,2017.
[29]谢帆,刘雪丽,肖卓妮,等.埃兹蛋白在延迟着床小鼠子宫内的表达及雌孕激素对其的调节作用[J].武汉大学学报(医学版).2015,36(5):702-705+723.
[30]黄伟,谢青贞,吴莲芝,等.埃兹蛋白在早孕自然流产患者蜕膜、绒毛中的表达[J]生殖医学杂志,2013,22(12):941-944.
[31]Quan C,Yan Y,Qin Z,et al.Ezrin regulates skin fibroblast size/mechanical properties and YAP-dependent proliferation[J].J cell Commun Signal,2007.
[32]Wei Li,William W.Jin,Kenji Tsuji,et al.Ezrin directly interacts with AQP2and promotes its endocytosis[J].J Cell Sci,2017,130:2914-2925.