群体感应调控干腌肉制品中乳酸菌环境胁迫应激机制研究进展
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摘要
乳酸菌作为干腌肉制品生产过程中的主要微生物之一,对制品品质的形成和安全性具有重要意义,而如何耐受腌腊过程中的环境胁迫是乳酸菌生存的基本要求。已有研究表明,LuxS/AI-2介导的群体感应(quorum-sensing,QS)系统能够调节乳酸菌在高盐、高酸等特定环境下AI-2的活性和luxS的转录水平。本文中,笔者主要综述了干腌肉制品中常见乳酸菌、乳酸菌群体感应系统及其分类以及群体感应调控乳酸菌环境胁迫应激机制,并对今后的研究方向进行了展望,以期为乳酸菌群体感应调控的干腌肉制品品质及其质量安全控制提供参考。
As one of the main microorganisms in the production process of dry-cured meat products,lactic acid bacteria are of great significance to the formation of product quality and the safety of products.How to withstand the environmental stress in the process of salting is the basic requirement for the survival of lactic acid bacteria.LuxS/AI-2 mediated quorum-sensing(QS) system can regulate the activity of AI-2 and the transcription level of luxS in lactic acid bacteria in specific environments such as high salt and high acid.In this paper,we review the common lactic acid bacteria strains in dry-cured meat products,lactic acid bacteria quorum sensing system and classification in dry-cured meat products,as well as the quorum sensing regulation mechanism of lactic acid bacteria environmental stress,and prospect for further research directions,in order to provide reference for quality and safety control of cured meat products through lactic acid bacteria QS regulation.
As one of the main microorganisms in the production process of dry-cured meat products,lactic acid bacteria are of great significance to the formation of product quality and the safety of products.How to withstand the environmental stress in the process of salting is the basic requirement for the survival of lactic acid bacteria.LuxS/AI-2 mediated quorum-sensing(QS) system can regulate the activity of AI-2 and the transcription level of luxS in lactic acid bacteria in specific environments such as high salt and high acid.In this paper,we review the common lactic acid bacteria strains in dry-cured meat products,lactic acid bacteria quorum sensing system and classification in dry-cured meat products,as well as the quorum sensing regulation mechanism of lactic acid bacteria environmental stress,and prospect for further research directions,in order to provide reference for quality and safety control of cured meat products through lactic acid bacteria QS regulation.
引文
[1] 范子超,杨延辰,丁有河,等.我国干腌火腿生产技术现状及展望[J].肉类工业,2019(1):48-51.
[2] 张一凡,李昌辉,李平兰,等.低盐发酵香肠用优良菌株的分离筛选及鉴定[J].肉类研究,2018,32(8):14-21.
[3] 赵小茜,魏旭丹,陈戴玲,等.乳酸菌耐酸耐胆盐机制研究进展[J].乳业科学与技术.2017,40(3):33-36.
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[5] 赖柏民,汪美贞,沈东升.细菌群体感应“合作-欺骗”研究进展[J].应用生态学报,2017,28(5):1735-1742.
[6] 孙锋,严慧聪,汪美贞.细菌群体感应调控多样性及群体感应淬灭[J].微生物学报,2019,59(3):454-467.
[7] Tanet L,Tamburini C,Baumas C,et al.Bacterial bioluminescence:light emission in photobacterium phosphoreum is not under quorum-sensing control[J].Front Microbiol,2019,10:365.
[8] OKUTSU N,MOROHOSHI T,XIE X,et al.Characterization of N-acylhomoserine lactones produced by bacteria isolated from industrial cooling water systems[J].Sensors,2015,16(1):E44.
[9] MARTíNEZ L C,VADYVALOO V.Mechanisms of post-transcriptional gene regulation in bacterial biofilms[J].Front Cell Infect Microbiol,2014,4(4):38-76.
[10] ALAYANDE A B,AUNG M M,KIM I S.Correlation between quorum sensing signal molecules and Pseudomonas aeruginosa′s biofilm development and virulency[J].Curr Microbiol,2018,75(7):787-793.
[11] LA ROSA S L,SOLHEIM M,DIEP D B,et al.Bioluminescence based biosensors for quantitative detection of enterococcal peptide-pheromone activity reveal inter-strain telesensing in vivo during polymicrobial systemic infection[J].Sci Rep,2015,5:8339.
[12] ZHANG H,WANG S,WANG Y.Functional overlap of hetP and hetZ in regulation of heterocyst differentiation in Anabaena sp.strain PCC 7120[J].J Bacteriol,2018,200(9):eD0707-17.
[13] STEIN E,SCHIKORA A.Detection of bacterial quorum sensing molecules[J].Methods Mol Biol,2018,1734:171-179.
[14] WANG D,LEE S H,SEEVE C,et al.Roles of the Gac-Rsm pathway in the regulation of phenazine biosynthesis in Pseudomonas chlororaphis 30-84[J].Microbiol Open,2013,2(3):505-524.
[15] 施安辉,周波.乳酸菌分类、生理特性及在食品酿造工业上的应用[J].中国调味品,2001(11):3-8.
[16] 曾令彬,谭汝成,熊善柏.腌腊鱼加工中优势乳酸菌的分离与鉴定[J].食品工业科技,2007(1):115-116,119.
[17] 冯秀娟,刘成国,娄爱华,等.湖南腊肉中优势菌种的筛选及初步鉴定[J].中国酿造,2012,31(5):127-130.
[18] 章德法,徐为民,徐幸莲.侗族发酵酸肉中乳酸菌的筛选及鉴定[J].江苏农业学报,2008,24(6):986-988.
[19] 刘正行,岳喜庆.腌虾蛄中优势乳酸菌的分离筛选与鉴定[J].食品科学,2015,36(9):125-129.
[20] LANDETA G,CURIEL J A,CARRASCOSA A V,et al.Technological and safety properties of lactic acid bacteria isolated from Spanish dry-cured sausages[J].Meat Sci,2013,95(2):272-280.
[21] 杨士章,张焕新,施帅,等.板鸭中优势乳酸菌的分离、筛选及鉴定[J].食品科学,2010,31(7):242-246.
[22] 王志航,冯雪,李树仁.细菌群体感应通讯系统淬灭及应用[J].药物生物技术,2018,25(5):70-74.
[23] SAUCIER L,POON A,STILES M E .Induction of bacteriocin in Carnobacterium piscicola LV17[J].J Appl Microbiol,1995,78(6):684-690.
[24] GALLOWAY W R J D,HODGKINSON J T,BOWDEN S D,et al.Quorum sensing in gram-negative bacteria:small-molecule modulation of AHL and AI-2 quorum sensing pathways[J].Chem Rev,2011,111(1):28-67..
[25] 邓曌曦,音建华,汪海峰.乳酸菌群体感应与其肠道生物膜形成的研究进展[J].微生物学报,2018,58(11):1970-1978.
[26] GE J,FANG B,WANG Y,et al.Bacillus subtilisenhances production of Paracin1.7,a bacteriocin produced by Lactobacillus paracasei HD1-7,isolated from Chinese fermented cabbage[J].Ann Microbiol,2014,64(4):1735-1743.
[27] BLANCHARD A E,LIAO C,LU T.An ecological understanding of quorum sensing-controlled bacteriocin synthesis[J].Cell Mol Bioengin,2016,9(3):1-12.
[28] PARK H,SHIN H,LEE K,et al.Autoinducer-2 properties of kimchi are associated with lactic acid bacteria involved in its fermentation[J].Int J Food Microbiol,2016,225:38-42.
[29] BLANA V A,NYCHAS G J E.Presence of quorum sensing signal molecules in minced beef stored under various temperature and packaging conditions[J].Int J Food Microbiol,2014,173:1-8.
[30] WEILAND-BR?UER N,Pinnow N,Schmitz R A,et al.Novel reporter for identification of interference with acyl homoserine lactone and autoinducer-2 quorum sensing[J].Appl Environ Microbiol,2015,81(4):1477-1489.
[31] LU L,HUME M E,PILLAI S D.Autoinducer-2-like activity associated with foods and its interaction with food additives[J].J Food Protect,2004,67(7):1457-1462.
[32] LI J,YANG X,SHI G,et al.Cooperation of lactic acid bacteria regulated by the luxS/AI-2 system involve in the biopreservation of refrigerated shrimp[J].Food Res Int,2018;45:62-69.
[33] MOSLEHI-JENABIAN S,VOGENSEN F K,JESPERSEN L.The quorum sensing luxS gene is induced in Lactobacillus acidophilus NCFM in response to Listeria monocytogenes[J].Int J Food Microbiol,2011,149(3):269-273.
[34] LEBEER S.Functional analysis of luxS in the probiotic strain Lactobacillus rhamnosus GG reveals a central metabolic role important for growth and biofilm formation[J].J.Bacteriol,2007,189(3):860-871.
[35] SUN Z,HE X,BRANCACCIO V F,et al.Bifidobacteria exhibit LuxS-dependent autoinducer 2 activity and biofilm formation[J].PLoS ONE,2014,9(2):e88260.
[36] 李博.不同生理环境胁迫对乳酸菌产信号分子AI-2影响的研究[D].呼和浩特:内蒙古农业大学 2016.
[37] 李洋,赵欣,张玉,等.牦牛酸乳中耐酸耐胆盐乳酸菌的分离筛选和鉴定[J].食品与机械,2018,34(7):23-28.
[38] 张其圣,陈功,申文熹,等.低盐泡菜乳酸菌群落演变及其优势菌群的探讨[J].中国食品学报,2018,18(9):109-119.
[39] AZCARATE-PERIL M A,MCAULIFFE O,ALTERMANN E,et al.Microarray analysis of a two-component regulatory system involved in acid resistance and proteolytic activity in Lactobacillus acidophilus[J].Appl Environ Microbiol,2005,71(10):5794-5804.
[40] MING T,HAN J,LI Y,et al.A metabolomics and proteomics study of the Lactobacillus plantarum in the grass carp fermentation[J].BMC Microbiol,2018,18(1):1-13.
[41] WALL T,BATH K,BRITTON R A,et al.The early response to acid shock in Lactobacillus reuteriInvolves the ClpL chaperone and a putative cell wall-altering esterase[J].Appl Environ Microbiol,2007,73(12):3924-3935.
[42] SZTAJER H,LEMME A,VILCHEZ R,et al.Autoinducer-2-regulated genes in Streptococcus mutans UA159 and global metabolic effect of the luxS mutation[J].J Bacteriol,2008,190(1):401-415.
[43] JIA F,ZHENG H,SUN S,et al.Role of luxS in stress tolerance and adhesion ability in Lactobacillus plantarum KLDS1.0391[J].Bio Med Res Int,2018(3):1-10.
[44] MOSLEHI-JENABIAN S,GORI K,JESPERSEN L.AI-2 signaling is induced by acidic shock in probiotic strains of Lactobacillus spp.[J].Int J Food Microbiol,2009,135(3):295-302.
[45] YEO S,PARK H,JI Y,et al.Influence of gastrointestinal stress on autoinducer-2 activity of two Lactobacillus species[J].FEMS Microbiol Ecol,2015;91(7):fiv065.
[46] LIN M,ZHOU G H,WANG Z G,et al.Functional analysis of LuxS/AI-2 from bacteria in Chinese fermented meat after high nitrate concentration shock[J].Eur Food Res Technol,2015,240(1):119-127.
[47] 顾悦.环境胁迫及酵母菌对乳酸菌LuxS/AI-2群体感应系统的影响[D].呼和浩特:内蒙古农业大学,2017.
[48] LIU L,WU R,ZHANG J,LI P.Overexpression of luxS promotes stress resistance and biofilm formation of Lactobacillus paraplantarum L-ZS9 by regulating the expression of multiple genes[J].Front Microbiol,2018,9:2628.
[49] 姜黎明,康子腾,柳陈坚,等.LuxS/AI-2群体感应系统介导乳酸杆菌益生特性研究进展[J].生命科学,2014,26(4):414-418.
[50] 张筠,孟祥晨.乳酸菌的胁迫应答及其对碳水化合物代谢的影响[J].中国食品学报,2017,17(6):145-151.
[51] GU Y,LI B,TIAN J,et al.The response of LuxS/AI-2 quorum sensing in Lactobacillus fermentum 2-1 to changes in environmental growth conditions[J].Ann Microbiol,2018,68(5):287-294.
[2] 张一凡,李昌辉,李平兰,等.低盐发酵香肠用优良菌株的分离筛选及鉴定[J].肉类研究,2018,32(8):14-21.
[3] 赵小茜,魏旭丹,陈戴玲,等.乳酸菌耐酸耐胆盐机制研究进展[J].乳业科学与技术.2017,40(3):33-36.
[4] 赵亚荣,张文羿,孙天松.乳酸菌环境胁迫应答分子机制研究进展[J].中国乳品工业,2014,42(4):42-45.
[5] 赖柏民,汪美贞,沈东升.细菌群体感应“合作-欺骗”研究进展[J].应用生态学报,2017,28(5):1735-1742.
[6] 孙锋,严慧聪,汪美贞.细菌群体感应调控多样性及群体感应淬灭[J].微生物学报,2019,59(3):454-467.
[7] Tanet L,Tamburini C,Baumas C,et al.Bacterial bioluminescence:light emission in photobacterium phosphoreum is not under quorum-sensing control[J].Front Microbiol,2019,10:365.
[8] OKUTSU N,MOROHOSHI T,XIE X,et al.Characterization of N-acylhomoserine lactones produced by bacteria isolated from industrial cooling water systems[J].Sensors,2015,16(1):E44.
[9] MARTíNEZ L C,VADYVALOO V.Mechanisms of post-transcriptional gene regulation in bacterial biofilms[J].Front Cell Infect Microbiol,2014,4(4):38-76.
[10] ALAYANDE A B,AUNG M M,KIM I S.Correlation between quorum sensing signal molecules and Pseudomonas aeruginosa′s biofilm development and virulency[J].Curr Microbiol,2018,75(7):787-793.
[11] LA ROSA S L,SOLHEIM M,DIEP D B,et al.Bioluminescence based biosensors for quantitative detection of enterococcal peptide-pheromone activity reveal inter-strain telesensing in vivo during polymicrobial systemic infection[J].Sci Rep,2015,5:8339.
[12] ZHANG H,WANG S,WANG Y.Functional overlap of hetP and hetZ in regulation of heterocyst differentiation in Anabaena sp.strain PCC 7120[J].J Bacteriol,2018,200(9):eD0707-17.
[13] STEIN E,SCHIKORA A.Detection of bacterial quorum sensing molecules[J].Methods Mol Biol,2018,1734:171-179.
[14] WANG D,LEE S H,SEEVE C,et al.Roles of the Gac-Rsm pathway in the regulation of phenazine biosynthesis in Pseudomonas chlororaphis 30-84[J].Microbiol Open,2013,2(3):505-524.
[15] 施安辉,周波.乳酸菌分类、生理特性及在食品酿造工业上的应用[J].中国调味品,2001(11):3-8.
[16] 曾令彬,谭汝成,熊善柏.腌腊鱼加工中优势乳酸菌的分离与鉴定[J].食品工业科技,2007(1):115-116,119.
[17] 冯秀娟,刘成国,娄爱华,等.湖南腊肉中优势菌种的筛选及初步鉴定[J].中国酿造,2012,31(5):127-130.
[18] 章德法,徐为民,徐幸莲.侗族发酵酸肉中乳酸菌的筛选及鉴定[J].江苏农业学报,2008,24(6):986-988.
[19] 刘正行,岳喜庆.腌虾蛄中优势乳酸菌的分离筛选与鉴定[J].食品科学,2015,36(9):125-129.
[20] LANDETA G,CURIEL J A,CARRASCOSA A V,et al.Technological and safety properties of lactic acid bacteria isolated from Spanish dry-cured sausages[J].Meat Sci,2013,95(2):272-280.
[21] 杨士章,张焕新,施帅,等.板鸭中优势乳酸菌的分离、筛选及鉴定[J].食品科学,2010,31(7):242-246.
[22] 王志航,冯雪,李树仁.细菌群体感应通讯系统淬灭及应用[J].药物生物技术,2018,25(5):70-74.
[23] SAUCIER L,POON A,STILES M E .Induction of bacteriocin in Carnobacterium piscicola LV17[J].J Appl Microbiol,1995,78(6):684-690.
[24] GALLOWAY W R J D,HODGKINSON J T,BOWDEN S D,et al.Quorum sensing in gram-negative bacteria:small-molecule modulation of AHL and AI-2 quorum sensing pathways[J].Chem Rev,2011,111(1):28-67..
[25] 邓曌曦,音建华,汪海峰.乳酸菌群体感应与其肠道生物膜形成的研究进展[J].微生物学报,2018,58(11):1970-1978.
[26] GE J,FANG B,WANG Y,et al.Bacillus subtilisenhances production of Paracin1.7,a bacteriocin produced by Lactobacillus paracasei HD1-7,isolated from Chinese fermented cabbage[J].Ann Microbiol,2014,64(4):1735-1743.
[27] BLANCHARD A E,LIAO C,LU T.An ecological understanding of quorum sensing-controlled bacteriocin synthesis[J].Cell Mol Bioengin,2016,9(3):1-12.
[28] PARK H,SHIN H,LEE K,et al.Autoinducer-2 properties of kimchi are associated with lactic acid bacteria involved in its fermentation[J].Int J Food Microbiol,2016,225:38-42.
[29] BLANA V A,NYCHAS G J E.Presence of quorum sensing signal molecules in minced beef stored under various temperature and packaging conditions[J].Int J Food Microbiol,2014,173:1-8.
[30] WEILAND-BR?UER N,Pinnow N,Schmitz R A,et al.Novel reporter for identification of interference with acyl homoserine lactone and autoinducer-2 quorum sensing[J].Appl Environ Microbiol,2015,81(4):1477-1489.
[31] LU L,HUME M E,PILLAI S D.Autoinducer-2-like activity associated with foods and its interaction with food additives[J].J Food Protect,2004,67(7):1457-1462.
[32] LI J,YANG X,SHI G,et al.Cooperation of lactic acid bacteria regulated by the luxS/AI-2 system involve in the biopreservation of refrigerated shrimp[J].Food Res Int,2018;45:62-69.
[33] MOSLEHI-JENABIAN S,VOGENSEN F K,JESPERSEN L.The quorum sensing luxS gene is induced in Lactobacillus acidophilus NCFM in response to Listeria monocytogenes[J].Int J Food Microbiol,2011,149(3):269-273.
[34] LEBEER S.Functional analysis of luxS in the probiotic strain Lactobacillus rhamnosus GG reveals a central metabolic role important for growth and biofilm formation[J].J.Bacteriol,2007,189(3):860-871.
[35] SUN Z,HE X,BRANCACCIO V F,et al.Bifidobacteria exhibit LuxS-dependent autoinducer 2 activity and biofilm formation[J].PLoS ONE,2014,9(2):e88260.
[36] 李博.不同生理环境胁迫对乳酸菌产信号分子AI-2影响的研究[D].呼和浩特:内蒙古农业大学 2016.
[37] 李洋,赵欣,张玉,等.牦牛酸乳中耐酸耐胆盐乳酸菌的分离筛选和鉴定[J].食品与机械,2018,34(7):23-28.
[38] 张其圣,陈功,申文熹,等.低盐泡菜乳酸菌群落演变及其优势菌群的探讨[J].中国食品学报,2018,18(9):109-119.
[39] AZCARATE-PERIL M A,MCAULIFFE O,ALTERMANN E,et al.Microarray analysis of a two-component regulatory system involved in acid resistance and proteolytic activity in Lactobacillus acidophilus[J].Appl Environ Microbiol,2005,71(10):5794-5804.
[40] MING T,HAN J,LI Y,et al.A metabolomics and proteomics study of the Lactobacillus plantarum in the grass carp fermentation[J].BMC Microbiol,2018,18(1):1-13.
[41] WALL T,BATH K,BRITTON R A,et al.The early response to acid shock in Lactobacillus reuteriInvolves the ClpL chaperone and a putative cell wall-altering esterase[J].Appl Environ Microbiol,2007,73(12):3924-3935.
[42] SZTAJER H,LEMME A,VILCHEZ R,et al.Autoinducer-2-regulated genes in Streptococcus mutans UA159 and global metabolic effect of the luxS mutation[J].J Bacteriol,2008,190(1):401-415.
[43] JIA F,ZHENG H,SUN S,et al.Role of luxS in stress tolerance and adhesion ability in Lactobacillus plantarum KLDS1.0391[J].Bio Med Res Int,2018(3):1-10.
[44] MOSLEHI-JENABIAN S,GORI K,JESPERSEN L.AI-2 signaling is induced by acidic shock in probiotic strains of Lactobacillus spp.[J].Int J Food Microbiol,2009,135(3):295-302.
[45] YEO S,PARK H,JI Y,et al.Influence of gastrointestinal stress on autoinducer-2 activity of two Lactobacillus species[J].FEMS Microbiol Ecol,2015;91(7):fiv065.
[46] LIN M,ZHOU G H,WANG Z G,et al.Functional analysis of LuxS/AI-2 from bacteria in Chinese fermented meat after high nitrate concentration shock[J].Eur Food Res Technol,2015,240(1):119-127.
[47] 顾悦.环境胁迫及酵母菌对乳酸菌LuxS/AI-2群体感应系统的影响[D].呼和浩特:内蒙古农业大学,2017.
[48] LIU L,WU R,ZHANG J,LI P.Overexpression of luxS promotes stress resistance and biofilm formation of Lactobacillus paraplantarum L-ZS9 by regulating the expression of multiple genes[J].Front Microbiol,2018,9:2628.
[49] 姜黎明,康子腾,柳陈坚,等.LuxS/AI-2群体感应系统介导乳酸杆菌益生特性研究进展[J].生命科学,2014,26(4):414-418.
[50] 张筠,孟祥晨.乳酸菌的胁迫应答及其对碳水化合物代谢的影响[J].中国食品学报,2017,17(6):145-151.
[51] GU Y,LI B,TIAN J,et al.The response of LuxS/AI-2 quorum sensing in Lactobacillus fermentum 2-1 to changes in environmental growth conditions[J].Ann Microbiol,2018,68(5):287-294.