丹绿补肾胶囊质量控制方法研究
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摘要
目的:建立丹绿补肾胶囊质量控制方法。方法:采用薄层色谱法(TLC)对丹绿补肾胶囊中白花丹、绿包藤、射干及胡椒药材进行定性鉴别;采用高效液相色谱法(HPLC),以次野鸢尾黄素作为指标成分进行定量测定。结果:薄层色谱法能清晰地鉴别丹绿补肾胶囊中的白花丹、绿包藤、射干及胡椒,阴性无干扰,专属性强;次野鸢尾黄素在1. 097～32. 91μg/m L (r2=0. 9999)质量浓度范围内与峰面积具有良好的线性关系;平均回收率(n=9)为100. 6%,RSD为1. 28%。结论:所建立的方法准确、可靠、专属性强,可作为丹绿补肾胶囊的质量控制方法。
Objective To establish the method of quality control of Danlv Bushen Capsules. Methods Plumbago zeylanica L.,Tinospora crispa( Linn.) Hook. f. & Thoms.,Belamcanda chinensis( L.) DC,Piper nigrum L. in Danlv Bushen Capsules. were identified by TLC,and Irisflorentin as index components was determined by HPLC. Results Danlv Bushen Capsules could be detected by TLC,The linearity ranges obtained from the calibration curves of Irisflorentin was 1. 097 ～ 32. 91 μg/m L( r2= 0. 9999). The average recoveries of Irisflorentin was 100. 6%( n = 9) and RSD was 1. 28%. Conclusion This method is simple,accurate,selective and highly reproducible. It can be used for the quality control of Danlv Bushen Capsules.
Objective To establish the method of quality control of Danlv Bushen Capsules. Methods Plumbago zeylanica L.,Tinospora crispa( Linn.) Hook. f. & Thoms.,Belamcanda chinensis( L.) DC,Piper nigrum L. in Danlv Bushen Capsules. were identified by TLC,and Irisflorentin as index components was determined by HPLC. Results Danlv Bushen Capsules could be detected by TLC,The linearity ranges obtained from the calibration curves of Irisflorentin was 1. 097 ～ 32. 91 μg/m L( r2= 0. 9999). The average recoveries of Irisflorentin was 100. 6%( n = 9) and RSD was 1. 28%. Conclusion This method is simple,accurate,selective and highly reproducible. It can be used for the quality control of Danlv Bushen Capsules.
引文
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[11]曹岳华,彭国庆.高效液相色谱法测定射干利咽口服液中射干苷、次野鸢尾黄素的含量[J].中南药学,2010,8(6):431-434.
[2]刘倩,陈金月,文隽,等.麻杏二陈汤煮散质量标准[J].中国实验方剂学杂志,2015,21(9):71-73.
[3]刘韶,雷鹏,李新中,等.芪黄颗粒的薄层鉴别[J].湖南中医学院学报,2004,24(1):26-27.
[4]田成旺,郭建华,张科,等.射黄含片的质量标准研究[J].中成药,2013,35(2):302-305.
[5]柯发敏,张开莲,钟明镜.咽炎含片中药材的薄层色谱研究[J].中药与临床,2010,1(3):27-28.
[6]国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2015:285.
[7]李欣妍,邹桂欣,尤献民,等.气管炎丸中野鸢尾黄素含量测定[J].辽宁中医杂志,2016,43(7):1450-1452.
[8]王晓月,邸子真,王光函,等.黄芩射干汤中千层纸素A和次野鸢尾黄素的含量测定[J].实用药物与临床,2017,20(5):563-565.
[9]邹桂欣,尤献民,王光函,等.野鸢尾中异黄酮与其抗炎作用的相关性分析[J].中华中医药学刊,2016,34(10):2406-2408.
[10]李锁,张晓瑞,尤献民,等.小儿咽扁颗粒中野鸢尾黄素和白射干素含量测定方法研究[J].辽宁中医杂志,2015,42(9):1733-1735.
[11]曹岳华,彭国庆.高效液相色谱法测定射干利咽口服液中射干苷、次野鸢尾黄素的含量[J].中南药学,2010,8(6):431-434.