鄂西南地区稻瘟病菌最适生长培养基筛选
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摘要
[目的]筛选鄂西南地区稻瘟病菌的最适生长培养基。[方法]从鄂西南地区稻瘟病菌生理小种文库中选择优势菌株,在米糠培养基、燕麦培养基、马铃薯培养基,以及以上3种材料按比例混配的4种组合培养基上进行培养,观察其生长状态。[结果]同种菌株在不同培养基上生长速率不同,不同菌株在同一种培养基上生长速率不同,在含有米糠、燕麦、马铃薯3种成分的M7培养基上稻瘟菌生长量最大。[结论]M7培养基为鄂西南地区稻瘟菌的最适生长培养基。
[Objective]To screen the optimum growth medium for Magnaporthe oryzae in southwestern Hubei Province. [Method] The dominant strains were selected from the physiological race library of Magnaporthe oryzea in southwestern Hubei Province,and cultured on rice bran medium,oatmeal medium,potato medium,and four kinds of combined medium in which the above three materials were mixed in proportion.[Result]The same strain showed different growth rates on different mediums,and different strains showed different growth rates on the same medium. The growth of Magnaporthe oryzae was the highest in M7 medium containing rice bran,oatmeal and potato. [Conclusion]M7medium was the optimum growth medium for Magnaporthe oryzae in southwestern Hubei Province.
[Objective]To screen the optimum growth medium for Magnaporthe oryzae in southwestern Hubei Province. [Method] The dominant strains were selected from the physiological race library of Magnaporthe oryzea in southwestern Hubei Province,and cultured on rice bran medium,oatmeal medium,potato medium,and four kinds of combined medium in which the above three materials were mixed in proportion.[Result]The same strain showed different growth rates on different mediums,and different strains showed different growth rates on the same medium. The growth of Magnaporthe oryzae was the highest in M7 medium containing rice bran,oatmeal and potato. [Conclusion]M7medium was the optimum growth medium for Magnaporthe oryzae in southwestern Hubei Province.
引文
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[13]霍秀娟,李朝生,韩美丽,等.灰梨孢菌产孢培养基筛选[J].南方农业学报,2012,43(5):605-608.
[14]张亚玲,靳学慧.不同稻瘟病菌菌株在PDA培养基上生长差异分析[J].中国农学通报,2005,21(10):295-298.
[15]王宝华,郑武,鲁国东,等.稻瘟菌产孢培养基的筛选[J].福建农业科技,2000(2):1-2.
[16]吴斯骏,孙强,陈洪凡,等.一种稻瘟病菌培养产孢培养基的配制与效比分析[J].安徽农业科学,2018,46(18):118-120.
[17]孙国昌,申宗坦,孙漱沅.稻瘟病菌产孢条件的研究[J].浙江农业大学学报,1990,16(1):51-54.
[2]吴双清,王林,卿明凤.水稻区试品种抗稻瘟病鉴定结果分析[J].中国稻米,2011,17(4):53-55.
[3]姚燕.湖北省建始县水稻稻瘟病的成因及防治[J].北京农业,2015(3):107.
[4]李求文,杨隆维,吴双清,等.武陵山区水稻抗稻瘟病品种的选育与利用[J].种子,2008,27(3):95-97.
[5]伍尚忠,朱小源,杨祁云,等.应用分子标记技术鉴证稻瘟病菌宗谱与寄主遗传背景的亲缘关系[J].中国农业科学,1999,32(6):56-62.
[6]田珂,杨武,李泌,等.鄂西南地区2012-2014年稻瘟病菌致病性变化分析[J].华中农业大学学报,2017,36(5):10-14.
[7]郭丽颖,赵宏伟,王敬国,等.黑龙江省稻瘟病菌生理小种鉴定和主栽水稻品种抗病性及遗传多样性分析[J].核农学报,2015,29(8):1444-1454.
[8]李亚,刘二明,戴良英,等.湖南稻瘟病菌群体遗传多样性与病菌致病型的关系[J].中国水稻科学,2007,21(3):304-308.
[9]姜于兰,谭萍,向红琼.贵州省稻瘟病菌遗传谱系与生理小种关系[J].湖北农业科学,2011,50(12):2438-2441.
[10]许鸣一.稻瘟病菌四种生理小种的形态和生长特性研究[D].哈尔滨:哈尔滨工业大学,2011.
[11]龙子文,周杰,王春台,等.鄂西地区稻瘟病菌生理小种文库的构建及初步鉴定[J].安徽农业科学,2012,40(24):12065-12067.
[12]兰波,李湘民,杨迎青,等.稻瘟病菌产孢技术研究[J].江西农业学报,2012,24(2):74-76.
[13]霍秀娟,李朝生,韩美丽,等.灰梨孢菌产孢培养基筛选[J].南方农业学报,2012,43(5):605-608.
[14]张亚玲,靳学慧.不同稻瘟病菌菌株在PDA培养基上生长差异分析[J].中国农学通报,2005,21(10):295-298.
[15]王宝华,郑武,鲁国东,等.稻瘟菌产孢培养基的筛选[J].福建农业科技,2000(2):1-2.
[16]吴斯骏,孙强,陈洪凡,等.一种稻瘟病菌培养产孢培养基的配制与效比分析[J].安徽农业科学,2018,46(18):118-120.
[17]孙国昌,申宗坦,孙漱沅.稻瘟病菌产孢条件的研究[J].浙江农业大学学报,1990,16(1):51-54.