丝状真菌产纤维素酶相关碳代谢阻遏因子的研究进展?
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摘要
简述了酵母和丝状真菌中普遍存在的一种碳代谢抑制因子CRE(CreA、CreB、CreC、CreD和Cre1、Cre2、Cre3、Cre4);利用纤维素所必须的锌双核簇转录因子CLR-1,负责大部分纤维素酶基因表的CLR-2和CLR-1的活性阻遏者CLR-3;真菌响应外界环境有重要作用的VIB1以及协调初级代谢、碳代谢阻遏和真菌生长重要参与者cAMP依赖蛋白激酶A(PkaA)在丝状真菌纤维素酶生产中的调控方式及相关联系以及它们在不同的丝状真菌中的影响,进而综述它们对丝状真菌生产纤维素酶的影响。
This paper briefly describes a carbon metabolism inhibitor CRE(CreA, CreB, CreC, CreD and Cre1,Cre2, Cre3, Cre4), which is ubiquitous in yeast and filamentous fungi. The zinc dual-core cluster transcription factor CLR-1, which is essential for the use of cellulose. Responsible for most cellulase gene expression is CLR-2 and CLR-1 active repressor is CLR-3. VIB1 play an important role in fungi respond to the external environment.cAMP-dependent protein kinase A(PkaA) is involved in the coordination of primary metabolism, carbon metabolism repression and fungal growth. And their related relationships and their effects in different filamentous fungi were briefly described. And then review their effects on the production of cellulase by filamentous fungi.
This paper briefly describes a carbon metabolism inhibitor CRE(CreA, CreB, CreC, CreD and Cre1,Cre2, Cre3, Cre4), which is ubiquitous in yeast and filamentous fungi. The zinc dual-core cluster transcription factor CLR-1, which is essential for the use of cellulose. Responsible for most cellulase gene expression is CLR-2 and CLR-1 active repressor is CLR-3. VIB1 play an important role in fungi respond to the external environment.cAMP-dependent protein kinase A(PkaA) is involved in the coordination of primary metabolism, carbon metabolism repression and fungal growth. And their related relationships and their effects in different filamentous fungi were briefly described. And then review their effects on the production of cellulase by filamentous fungi.
引文
[1]Marcelo K,John E M,Simon D S,et al.Structural characterization of a unique marine animal family 7 cellobiohydrolase suggests a mechanism of cellulase salt tolerance[J].Proceedings of the National Academy of Sciences of the United States of America,2013,110(25):10189-10194.
[2]董妙音,王曙阳,王雨辰,等.丝状真菌在纤维素酶合成过程中碳源代谢调控的研究进展[J].中国酿造,2016,35(10):1-4.
[3]Glass N L,Schmoll M,Cate J H,et al.Plant cell wall deconstruction by ascomycete fungi[J].Annual Review of Microbiology,2013,67(1):477-498.
[4]Amore A,Giacobbe S,Faraco V.Regulation of cellulase and hemicellulase gene expression in fungi[J].Current Genomics,2013,14(4):230-249.
[5]Carle-Urioste J C,Escobar-Vera J,El-Gogary S,et al.Cellulase induction in Trichoderma reesei by cellulose requires its own basal expression[J].Journal of Biological Chemistry,1997,272(15):10169-10174.
[6]林良才,李金根,王邦,等.粗糙脉孢菌木质纤维素降解利用研究进展[J].生物加工过程,2014(1):28-36.
[7]Gielkens M M C,Dekkers E,Visser J,et al.Two cellobiohydrolase-encoding genes from aspergillus niger require d-Xylose and the Xylanolytic transcriptional activator XlnR for their expression[J].Applied&Environmental Microbiology,1999,65(10):4340-4345.
[8]李辉,王义强,陈介南,等.里氏木霉产纤维素酶的诱导和合成机理研究进展[J].中国酿造,2011,30(6):8-12.
[9]吴艳,顾阳,任聪,等.微生物分解代谢物控制蛋白CcpA的研究进展[J].生命科学,2011,23(9):882-890.
[10]Rolland F,Winderickx J,Thevelein J M.Glucose-sensing and-signalling mechanisms in yeast[J].Fems Yeast Research,2002,2(2):183-201.
[11]Huberman L B,Coradetti S T,Glass N L.Network of nutrient-sensing pathways and a conserved kinase cascade integrate osmolarity and carbon sensing in Neurospora crassa[J].Proc Natl Acad Sci U S A,2017,114(41):E8665-E8674.
[12]Coradetti S T,Craig J P,Xiong Y,et al.Conserved and essential transcription factors for cellulase gene expression in ascomycete fungi[J].Proceedings of the National Academy of Sciences of the United States of America,2012,109(19):7397-7402.
[13]Coradetti S T,Xiong Y,Glass N L.Analysis of a conserved cellulase transcriptional regulator reveals inducer-independent production of cellulolytic enzymes in Neurospora crassa[J].Microbiologyopen,2013,2(4):595-609.
[14]Craig J P,Coradetti S T,Starr T L,et al.Direct target network of the Neurospora crassa plant cell wall deconstruction regulators CLR-1,CLR-2,and XLR-1[J].Mbio,2015,6(5):e01452-15.
[15]Coradetti S T,Xiong Y,Glass N L.Analysis of a conserved cellulase transcriptional regulator reveals inducer-independent production of cellulolytic enzymes in Neurospora crassa[J].Microbiologyopen,2013,2(4):595-609.
[16]Ogawa M,Kobayashi T,Koyama Y.Man R,a transcriptional regulator of theβ-mannan utilization system,controls the cellulose utilization system in Aspergillus oryzae[J].Journal of the Agricultural Chemical Society of Japan,2013,77(2):426-429.
[17]Matsuura T,Dovzhenok A A,Coradetti S T,et al.Synthetic gene network with positive feedback loop amplifies cellulase gene expression in Neurospora crassa[J].Acs Synthetic Biology,2018,7(5):1395-1405.
[18]Dementhon K I G G N.VIB-1 is required for expression of genes necessary for programmed cell death in Neurospora crassa[J].Eukaryot Cell,2006(5):2161-2173.
[19]Xiong Y,Sun J,Glass N L.VIB1,a link between glucose signaling and carbon catabolite repression,is essential for plant cell wall degradation by Neurospora crassa[J].Plos Genetics,2014,10(8):e1004500a.
[20]Wang Q,Lin H,Shen Q,et al.Characterization of cellulase secretion and Cre1-mediated carbon source repression in the potential lignocellulose-degrading strain Trichoderma asperellum T-1[J].Plos One,2015,10(3):e119237.
[21]Ilmén M,Thrane C,Penttil?M.The glucose repressor gene cre1 of Trichoderma:Isolation and expression of a full-length and a truncated mutant form[J].Molecular&General Genetics Mgg,1996,251(4):451-460.
[22]杨帆.转录因子CRE1及ACE1在嗜热毁丝霉纤维素酶基因表达调控中的作用[D].深圳大学,2015.
[23]Du Y Z B T.Production of cellulases by rhizopus stolonifer from glucose-containing media based on the regulation of transcriptional regulator CRE[J].Microbiol Biotechnol,2017,27(3):514-523.
[24]Cubero B,Scazzocchio C.Two different,adjacent and divergent zinc finger binding sites are necessary for CREA-mediated carbon catabolite repression in the proline gene cluster of Aspergillus nidulans[J].Embo Journal,1994,13(2):407-415.
[25]Macpherson S,Larochelle M,Turcotte B.A fungal family of transcriptional regulators:The zinc cluster proteins[J].Microbiology&Molecular Biology Reviews,2006,70(3):583-604.
[26]Yang F,Gong Y,Liu G,et al.Enhancing cellulase production in thermophilic fungus myceliophthora thermophila ATCC42464 by RNA interference of cre1 gene expression[J].J Microbiol Biotechnol,2015,25(7):1101-1107.
[27]周广麒,吕晶,李忠海,等.斜卧青霉去泛素化蛋白酶CREB的缺失提高纤维素酶的生产[J].生物工程学报,2012,28(8):959-972.
[28]Tamayo E N,Villanueva A,Hasper A A,et al.CreA mediates repression of the regulatory gene which controls the production of xylanolytic enzymes in Aspergillus nidulans[J].Fungal Genetics&Biology,2008,45(6):984-993.
[29]Lockington R,Kelly J.Carbon catabolite repression in Aspergillus nidulans involves deubiquitination[J].Molecular Microbiology,2001,40(6):1311-1321.
[30]Rogers S,Wells R,Rechsteiner M.Amino acid sequences common to rapidly degraded proteins:The PEST hypothesis[J].Science,1986,234(4774):364-368.
[31]Denton J A,Kelly J M.Disruption of Trichoderma reesei Cre2,encoding an ubiquitin C-terminal hydrolase,results in increased cellulase activity[J].Bmc Biotechnology,2011,11(1):1-10.
[32]Lockington R A,Kelly J M.The WD40-repeat protein CreC interacts with and stabilizes the deubiquitinating enzyme CreB in vivo in Aspergillus nidulans[J].Molecular Microbiology,2002,43(5):1173.
[33]Boase N A K J.A role for CreD,a carbon catabolite repression gene from Aspergillus nidulans,in ubiquitination[J].Mol Microbiol,2003,53(3):929-940.
[34]刘雯莉.cre4基因在里氏木霉中对纤维素酶表达的调控研究[D].深圳大学,2016.
[35]Mach-Aigner A R,Pucher M E,Steiger M G,et al.Transcriptional regulation of Xyr1,encoding the main regulator of the xylanolytic and cellulolytic enzyme system in Hypocrea jecorina[J].Applied&Environmental Microbiology,2008,74(21):6554-6562.
[36]陈小玲,陈东,张穗生,等.瑞氏木霉碳源阻遏相关基因Cre1的分子改造[J].基因组学与应用生物学,2014,33(2):288-292.
[37]Takashima S,Iikura H,Nakamura A,et al.Analysis of Cre1 binding sites in the Trichoderma reesei cbh1 upstream region[J].Fems Microbiology Letters,1996,145(3):361-366.
[38]Strauss J,Mach R L,Zeilinger S,et al.Crel,the carbon catabolite repressor protein from Trichoderma reesei[J].Febs Letters,1995,376(1-2):103-107.
[39]Sun J,Glass N L.Identification of the CRE-1 cellulolytic regulon in Neurospora crassa[J].Plos One,2011,6(9):e25654-68.
[40]Shi Y.Serine/threonine phosphatases:Mechanism through structure[J].Cell,2009,139(3):468-484.
[41]Hardie D G.AMP-activated/SNF1 protein kinases:Conserved guardians of cellular energy[J].Nat Rev Mol Cell Biol,2007,8(10):774-785.
[42]Broach J R.Nutritional control of growth and development in yeast[J].Genetics,2012,192(1):73-105.
[43]Leandro JoséD A,Laure Nicolas Annick R,Marcela S,et al.Aspergillus nidulans protein kinase A plays an important role in cellulase production[J].Biotechnology for biofuels,2015,8(1):213-233.
[44]Lafon A,Seo J A,Han K H,et al.The heterotrimeric G-protein GanB(alpha)-SfaD(beta)-GpgA(gamma)is a carbon source sensor involved in early cAMP-dependent germination in Aspergillus nidulans[J].Genetics,2005,171(1):71-80.
[45]Brown N A,Dos Reis T F,Ries L N,et al.G-protein coupled receptor-mediated nutrient sensing and developmental control in Aspergillus nidulans[J].Molecular Microbiology,2015,98(3):420-439.
[46]Fillinger S,Chaveroche M K,Shimizu K,et al.cAMP and ras signalling independently control spore germination in the filamentous fungus Aspergillus nidulans[J].Molecular Microbiology,2002,44(4):1001-1016.
[2]董妙音,王曙阳,王雨辰,等.丝状真菌在纤维素酶合成过程中碳源代谢调控的研究进展[J].中国酿造,2016,35(10):1-4.
[3]Glass N L,Schmoll M,Cate J H,et al.Plant cell wall deconstruction by ascomycete fungi[J].Annual Review of Microbiology,2013,67(1):477-498.
[4]Amore A,Giacobbe S,Faraco V.Regulation of cellulase and hemicellulase gene expression in fungi[J].Current Genomics,2013,14(4):230-249.
[5]Carle-Urioste J C,Escobar-Vera J,El-Gogary S,et al.Cellulase induction in Trichoderma reesei by cellulose requires its own basal expression[J].Journal of Biological Chemistry,1997,272(15):10169-10174.
[6]林良才,李金根,王邦,等.粗糙脉孢菌木质纤维素降解利用研究进展[J].生物加工过程,2014(1):28-36.
[7]Gielkens M M C,Dekkers E,Visser J,et al.Two cellobiohydrolase-encoding genes from aspergillus niger require d-Xylose and the Xylanolytic transcriptional activator XlnR for their expression[J].Applied&Environmental Microbiology,1999,65(10):4340-4345.
[8]李辉,王义强,陈介南,等.里氏木霉产纤维素酶的诱导和合成机理研究进展[J].中国酿造,2011,30(6):8-12.
[9]吴艳,顾阳,任聪,等.微生物分解代谢物控制蛋白CcpA的研究进展[J].生命科学,2011,23(9):882-890.
[10]Rolland F,Winderickx J,Thevelein J M.Glucose-sensing and-signalling mechanisms in yeast[J].Fems Yeast Research,2002,2(2):183-201.
[11]Huberman L B,Coradetti S T,Glass N L.Network of nutrient-sensing pathways and a conserved kinase cascade integrate osmolarity and carbon sensing in Neurospora crassa[J].Proc Natl Acad Sci U S A,2017,114(41):E8665-E8674.
[12]Coradetti S T,Craig J P,Xiong Y,et al.Conserved and essential transcription factors for cellulase gene expression in ascomycete fungi[J].Proceedings of the National Academy of Sciences of the United States of America,2012,109(19):7397-7402.
[13]Coradetti S T,Xiong Y,Glass N L.Analysis of a conserved cellulase transcriptional regulator reveals inducer-independent production of cellulolytic enzymes in Neurospora crassa[J].Microbiologyopen,2013,2(4):595-609.
[14]Craig J P,Coradetti S T,Starr T L,et al.Direct target network of the Neurospora crassa plant cell wall deconstruction regulators CLR-1,CLR-2,and XLR-1[J].Mbio,2015,6(5):e01452-15.
[15]Coradetti S T,Xiong Y,Glass N L.Analysis of a conserved cellulase transcriptional regulator reveals inducer-independent production of cellulolytic enzymes in Neurospora crassa[J].Microbiologyopen,2013,2(4):595-609.
[16]Ogawa M,Kobayashi T,Koyama Y.Man R,a transcriptional regulator of theβ-mannan utilization system,controls the cellulose utilization system in Aspergillus oryzae[J].Journal of the Agricultural Chemical Society of Japan,2013,77(2):426-429.
[17]Matsuura T,Dovzhenok A A,Coradetti S T,et al.Synthetic gene network with positive feedback loop amplifies cellulase gene expression in Neurospora crassa[J].Acs Synthetic Biology,2018,7(5):1395-1405.
[18]Dementhon K I G G N.VIB-1 is required for expression of genes necessary for programmed cell death in Neurospora crassa[J].Eukaryot Cell,2006(5):2161-2173.
[19]Xiong Y,Sun J,Glass N L.VIB1,a link between glucose signaling and carbon catabolite repression,is essential for plant cell wall degradation by Neurospora crassa[J].Plos Genetics,2014,10(8):e1004500a.
[20]Wang Q,Lin H,Shen Q,et al.Characterization of cellulase secretion and Cre1-mediated carbon source repression in the potential lignocellulose-degrading strain Trichoderma asperellum T-1[J].Plos One,2015,10(3):e119237.
[21]Ilmén M,Thrane C,Penttil?M.The glucose repressor gene cre1 of Trichoderma:Isolation and expression of a full-length and a truncated mutant form[J].Molecular&General Genetics Mgg,1996,251(4):451-460.
[22]杨帆.转录因子CRE1及ACE1在嗜热毁丝霉纤维素酶基因表达调控中的作用[D].深圳大学,2015.
[23]Du Y Z B T.Production of cellulases by rhizopus stolonifer from glucose-containing media based on the regulation of transcriptional regulator CRE[J].Microbiol Biotechnol,2017,27(3):514-523.
[24]Cubero B,Scazzocchio C.Two different,adjacent and divergent zinc finger binding sites are necessary for CREA-mediated carbon catabolite repression in the proline gene cluster of Aspergillus nidulans[J].Embo Journal,1994,13(2):407-415.
[25]Macpherson S,Larochelle M,Turcotte B.A fungal family of transcriptional regulators:The zinc cluster proteins[J].Microbiology&Molecular Biology Reviews,2006,70(3):583-604.
[26]Yang F,Gong Y,Liu G,et al.Enhancing cellulase production in thermophilic fungus myceliophthora thermophila ATCC42464 by RNA interference of cre1 gene expression[J].J Microbiol Biotechnol,2015,25(7):1101-1107.
[27]周广麒,吕晶,李忠海,等.斜卧青霉去泛素化蛋白酶CREB的缺失提高纤维素酶的生产[J].生物工程学报,2012,28(8):959-972.
[28]Tamayo E N,Villanueva A,Hasper A A,et al.CreA mediates repression of the regulatory gene which controls the production of xylanolytic enzymes in Aspergillus nidulans[J].Fungal Genetics&Biology,2008,45(6):984-993.
[29]Lockington R,Kelly J.Carbon catabolite repression in Aspergillus nidulans involves deubiquitination[J].Molecular Microbiology,2001,40(6):1311-1321.
[30]Rogers S,Wells R,Rechsteiner M.Amino acid sequences common to rapidly degraded proteins:The PEST hypothesis[J].Science,1986,234(4774):364-368.
[31]Denton J A,Kelly J M.Disruption of Trichoderma reesei Cre2,encoding an ubiquitin C-terminal hydrolase,results in increased cellulase activity[J].Bmc Biotechnology,2011,11(1):1-10.
[32]Lockington R A,Kelly J M.The WD40-repeat protein CreC interacts with and stabilizes the deubiquitinating enzyme CreB in vivo in Aspergillus nidulans[J].Molecular Microbiology,2002,43(5):1173.
[33]Boase N A K J.A role for CreD,a carbon catabolite repression gene from Aspergillus nidulans,in ubiquitination[J].Mol Microbiol,2003,53(3):929-940.
[34]刘雯莉.cre4基因在里氏木霉中对纤维素酶表达的调控研究[D].深圳大学,2016.
[35]Mach-Aigner A R,Pucher M E,Steiger M G,et al.Transcriptional regulation of Xyr1,encoding the main regulator of the xylanolytic and cellulolytic enzyme system in Hypocrea jecorina[J].Applied&Environmental Microbiology,2008,74(21):6554-6562.
[36]陈小玲,陈东,张穗生,等.瑞氏木霉碳源阻遏相关基因Cre1的分子改造[J].基因组学与应用生物学,2014,33(2):288-292.
[37]Takashima S,Iikura H,Nakamura A,et al.Analysis of Cre1 binding sites in the Trichoderma reesei cbh1 upstream region[J].Fems Microbiology Letters,1996,145(3):361-366.
[38]Strauss J,Mach R L,Zeilinger S,et al.Crel,the carbon catabolite repressor protein from Trichoderma reesei[J].Febs Letters,1995,376(1-2):103-107.
[39]Sun J,Glass N L.Identification of the CRE-1 cellulolytic regulon in Neurospora crassa[J].Plos One,2011,6(9):e25654-68.
[40]Shi Y.Serine/threonine phosphatases:Mechanism through structure[J].Cell,2009,139(3):468-484.
[41]Hardie D G.AMP-activated/SNF1 protein kinases:Conserved guardians of cellular energy[J].Nat Rev Mol Cell Biol,2007,8(10):774-785.
[42]Broach J R.Nutritional control of growth and development in yeast[J].Genetics,2012,192(1):73-105.
[43]Leandro JoséD A,Laure Nicolas Annick R,Marcela S,et al.Aspergillus nidulans protein kinase A plays an important role in cellulase production[J].Biotechnology for biofuels,2015,8(1):213-233.
[44]Lafon A,Seo J A,Han K H,et al.The heterotrimeric G-protein GanB(alpha)-SfaD(beta)-GpgA(gamma)is a carbon source sensor involved in early cAMP-dependent germination in Aspergillus nidulans[J].Genetics,2005,171(1):71-80.
[45]Brown N A,Dos Reis T F,Ries L N,et al.G-protein coupled receptor-mediated nutrient sensing and developmental control in Aspergillus nidulans[J].Molecular Microbiology,2015,98(3):420-439.
[46]Fillinger S,Chaveroche M K,Shimizu K,et al.cAMP and ras signalling independently control spore germination in the filamentous fungus Aspergillus nidulans[J].Molecular Microbiology,2002,44(4):1001-1016.