铁皮石斛茎、叶、花中黄酮含量及其体外抗氧化活性研究
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摘要
目的:探讨铁皮石斛不同部位黄酮含量以及其体外抗氧化活性,为其资源充分开发利用提供实验依据。方法:硝酸铝比色法测定黄酮含量,进行二苯基-2-三硝基苯肼(DPPH)自由基清除率、2′-联氨-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)自由基清除率以及超氧阴离子清除率的测定,评价体外抗氧化活性。结果:铁皮石斛茎、叶、花黄酮在不同的体外抗氧化体系中,均表现出了不同的抗氧化活性。其中铁皮石斛花黄酮在DPPH自由基清除率、ABTS自由基清除率以及超氧阴离子清除率的测定中均表现最优。结论:铁皮石斛茎、叶、花中黄酮类成分具有较高抗氧化活性,尤其是铁皮石斛花黄酮。
Objective:The flavonoids content in different parts of officinale Kimura & Migo and its antioxidant activity in vitro were investigated,which provided experimental basis for the full development and utilization of its resources.Methods:The content of flavonoids was determined by aluminium nitrate colorimetry.The antioxidant activity in vitro was evaluated by the free radical scavenging rate of diphenyl-2-trinitrophenylhydrazine(DPPH),2′-hydrazine-bis-3-ethylbenzothiazoline-6-sulfonic acid(ABTS) and superoxide anion scavenging rate.Results:Flavonoids from stems,leaves and flowers of officinale Kimura & Migo showed different antioxidant activities in different antioxidant systems in vitro.The flavonoids of officinale Kimura & Migo in flowers were the best in the determination of DPPH free radical scavenging rate,ABTS free radical scavenging rate and superoxide anion scavenging rate.Conclusion:Flavonoids in stems,leaves and flowers of officinale Kimura & Migo have high antioxidant activity,especially in flowers of officinale Kimura & Migo.The experimental results provide a theoretical basis for better development and utilization of officinale Kimura & Migo resources.
Objective:The flavonoids content in different parts of officinale Kimura & Migo and its antioxidant activity in vitro were investigated,which provided experimental basis for the full development and utilization of its resources.Methods:The content of flavonoids was determined by aluminium nitrate colorimetry.The antioxidant activity in vitro was evaluated by the free radical scavenging rate of diphenyl-2-trinitrophenylhydrazine(DPPH),2′-hydrazine-bis-3-ethylbenzothiazoline-6-sulfonic acid(ABTS) and superoxide anion scavenging rate.Results:Flavonoids from stems,leaves and flowers of officinale Kimura & Migo showed different antioxidant activities in different antioxidant systems in vitro.The flavonoids of officinale Kimura & Migo in flowers were the best in the determination of DPPH free radical scavenging rate,ABTS free radical scavenging rate and superoxide anion scavenging rate.Conclusion:Flavonoids in stems,leaves and flowers of officinale Kimura & Migo have high antioxidant activity,especially in flowers of officinale Kimura & Migo.The experimental results provide a theoretical basis for better development and utilization of officinale Kimura & Migo resources.
引文
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[14]谭榀新,叶涛,刘湘新,等.植物提取物抗氧化成分及机理研究进展[J].食品科学,2010,31(15):288-292.
[15]谢丽源,甘炳成,彭卫红,等.灵芝深层发酵产物抗氧化活性物质与抗氧化能力分析[J].食品工业科技,2015,36(2):105-109.
[2]聂少平,蔡海兰.铁皮石斛活性成分及其功能研究进展[J].食品科学,2012,33(23):356-361.
[3]张桂玲,冉靓,罗绪强.两种荔波产铁皮石斛多糖、氨基酸及微量元素含量分析[J].时珍国医国药,2017,28(2):296-298.
[4]吕朝耕,杨健,康传志,等.铁皮石斛中10种黄酮类成分UPLC-MS/MS测定与多糖组成含量分析[J].中国实验方剂学杂志,2017,23(17):47-52.
[5]宋燕华,蔡德雷,傅剑云,等.铁皮石斛叶对二代繁殖雌性大鼠免疫水平影响的研究[J].浙江预防医学,2016,28(2):109-112.
[6]陈存武,陈乃东,孟云飞,等.沉淀敲出法快速发现霍山产铁皮石斛生物碱及其组分抗氧化活性在线测定[J].天然产物研究与开发,2014,26(7):1000-1003,1013.
[7]唐静月,颜美秋,齐芳芳,等.铁皮石斛花总黄酮提取工艺优化及体外抗氧化活性研究[J].浙江中医药大学学报,2017,41(3):235-242.
[8]孙海峰,闫萍,闫佳驹.翻白草总黄酮纯化方法的研究[J].现代中药研究与实践,2017,31(1):41-43.
[9]侯敏娜.绞股蓝中总黄酮提取工艺的研究[J].现代中药研究与实践,2012,26(5):51-52.
[10]李汶潓,李亚鹤,徐年军,等.壳聚糖酶法降解工艺优化及其产物的抗氧化活性[J].中国食品学报,2017,17(11):121-130.
[11]赵翊君,刘华勇,陈森林,等.辣木籽酶解产物的蛋白回收率及DPPH·清除能力的研究[J].现代食品科技,2017,33(12):77-83.
[12]王金梅,姚辰,李昌勤,等.基于DPPH、ABTS和FRAP法的红花抗氧化谱效关系研究[J].中国药学杂志,2017,52(10):825-831.
[13]詹雁,王立珍,徐超群,等.马齿苋不同提取部位抗氧化活性的比较[J].中药药理与临床,2012,28(5):126-128.
[14]谭榀新,叶涛,刘湘新,等.植物提取物抗氧化成分及机理研究进展[J].食品科学,2010,31(15):288-292.
[15]谢丽源,甘炳成,彭卫红,等.灵芝深层发酵产物抗氧化活性物质与抗氧化能力分析[J].食品工业科技,2015,36(2):105-109.