去势对成年雄性斑胸草雀LMAN神经元电生理特性的影响
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摘要
以成年雄性斑胸草雀为研究对象,利用膜片钳技术观察去势前后LMAN(Lateral Magnocellular Nucleus of the Anterior Neostriatum)的电生理活动,进一步探究AFP通路与鸣曲的关系.去势后,LMAN神经元的膜时间常数变短,膜电阻减小,动作电位潜伏期缩短,动作电位后超极化幅值变低,同时动作电位发放频率变快.结果表明:去势后LMAN神经元兴奋性增强,使AFP通路更加活跃,去势引起的鸣曲不稳定可能是AFP通路活动增强所致.
To explore the link between AFP and song of adult male zebra finches,the effect of castration on electrophysiological properties of their LMAN neurons was investigated. Patch-clamp recording was used to record the electrophysiological properties of LMAN neurons. Membrane time constant,evoked AP latency,AHP peak amplitude and input resistance were found to decrease after castration. These indicate that castration increases excitability of LMAN neuron and song plasticity.
To explore the link between AFP and song of adult male zebra finches,the effect of castration on electrophysiological properties of their LMAN neurons was investigated. Patch-clamp recording was used to record the electrophysiological properties of LMAN neurons. Membrane time constant,evoked AP latency,AHP peak amplitude and input resistance were found to decrease after castration. These indicate that castration increases excitability of LMAN neuron and song plasticity.
引文
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[3] SOHRABJI F,NORDEEN E J,NORDEEN K W. Selective impairment of song learning following lesions of a forebrain nucleus in the juvenile zebra finch[J]. Behavioral&Neural Biology,1990,53(1):51-63.
[4] OLVECZKY B P,ANDALMAN A S,FEE M S. Vocal experimentation in the juvenile songbird requires a basal ganglia circuit[J]. PloS Biology,2005,3(5):e153/1-8.
[5] NORDEEN K W,NORDEEN E J. Deafening-induced vocal deterioration in adult songbirds is reversed by disrupting a basal ganglia-forebrain circuit[J]. The Journal of Neuroscience:the Official Journal of the Society for Neuroscience,2010,30(21):7392-7400.
[6] BRENOWITZ E A. Plasticity of the adult avian song control system[J]. Annals of the New York Academy of Sciences,2004,1016:560-585.
[7] SIZEMORE M,PERKEL D J. Premotor synaptic plasticity limited to the critical period for song learning[J]. Proceedings of the National Academy of Sciences of the United States of America,2011,108(42):17492-17497.
[8] WANG S,LIAO C,LI F,et al. Castration modulates singing patterns and electrophysiological properties of RA projection neurons in adult male zebra finches[J]. PeerJ,2014,2:e352/1-16.
[9]张近春,王松华,李东风.损毁LMAN逆转去势引起的成年雄鸟鸣曲变化[J].华南师范大学学报(自然科学版),2016,48(5):58-62.ZHANG J C,WANG S H,LI D F. Castrating-induced song change in adult male zebra finch(Taeniogygia guttata)is reversed by LMAN lesion[J]. Journal of South China Normal University(Natural Science Edition),2016,48(5):58-62.
[10] WANG S,MENG W,LIU S,et al. Sex differences of excitatory synaptic transmission in RA projection neurons of adult zebra finches[J]. Neuroscience Letters,2014,582:75-80.
[11] BOETTIGER C A,DOUPE A J. Intrinsic and thalamic excitatory inputs onto songbird LMAN neurons differ in their pharmacological and temporal properties[J]. Journal of Neurophysiology,1998,79(5):2615-2628.
[12] DOUPE A J,KUHL P K. Birdsong and human speech:common themes and mechanisms[J]. Annual Review of Neuroscience,1999,22:567-631.
[13] HERRMANN K,ARNOLD A P. The development of afferent projections to the robust archistriatal nucleus in male zebra finches:a quantitative electron microscopic study[J]. The Journal of Neuroscience:the Official Journal of the Society for Neuroscience,1991,11(7):2063-2074.
[14] KAO M H,DOUPE A J,BRAINARD M S. Contributions of an avian basal ganglia-forebrain circuit to real-time modulation of song[J]. Nature,2005,433(7026):638-643.
[15] LIVINGSTON F S,MOONEY R. Androgens and isolation from adult tutors differentially affect the development of songbird neurons critical to vocal plasticity[J]. Journal of Neurophysiology,2001,85(1):34-42.
[16] SINGH T D,BASHAM M E,NORDEEN E J,et al. Early sensory and hormonal experience modulate age-related changes in NR2B mRNA within a forebrain region controlling avian vocal learning[J]. Journal of Neurobiology,2000,44(1):82-94.
[17] SINGH T D,HEINRICH J E,WISSMAN A M,et al. Seasonal regulation of NMDA receptor NR2B mRNA in the adult canary song system[J]. Journal of Neurobiology,2003,54(4):593-603.
[2] BOTTJER S W,MIESNER E A,ARNOLD A P. Forebrain lesions disrupt development but not maintenance of song in passerine birds[J]. Science,1984,224(4651):901-903.
[3] SOHRABJI F,NORDEEN E J,NORDEEN K W. Selective impairment of song learning following lesions of a forebrain nucleus in the juvenile zebra finch[J]. Behavioral&Neural Biology,1990,53(1):51-63.
[4] OLVECZKY B P,ANDALMAN A S,FEE M S. Vocal experimentation in the juvenile songbird requires a basal ganglia circuit[J]. PloS Biology,2005,3(5):e153/1-8.
[5] NORDEEN K W,NORDEEN E J. Deafening-induced vocal deterioration in adult songbirds is reversed by disrupting a basal ganglia-forebrain circuit[J]. The Journal of Neuroscience:the Official Journal of the Society for Neuroscience,2010,30(21):7392-7400.
[6] BRENOWITZ E A. Plasticity of the adult avian song control system[J]. Annals of the New York Academy of Sciences,2004,1016:560-585.
[7] SIZEMORE M,PERKEL D J. Premotor synaptic plasticity limited to the critical period for song learning[J]. Proceedings of the National Academy of Sciences of the United States of America,2011,108(42):17492-17497.
[8] WANG S,LIAO C,LI F,et al. Castration modulates singing patterns and electrophysiological properties of RA projection neurons in adult male zebra finches[J]. PeerJ,2014,2:e352/1-16.
[9]张近春,王松华,李东风.损毁LMAN逆转去势引起的成年雄鸟鸣曲变化[J].华南师范大学学报(自然科学版),2016,48(5):58-62.ZHANG J C,WANG S H,LI D F. Castrating-induced song change in adult male zebra finch(Taeniogygia guttata)is reversed by LMAN lesion[J]. Journal of South China Normal University(Natural Science Edition),2016,48(5):58-62.
[10] WANG S,MENG W,LIU S,et al. Sex differences of excitatory synaptic transmission in RA projection neurons of adult zebra finches[J]. Neuroscience Letters,2014,582:75-80.
[11] BOETTIGER C A,DOUPE A J. Intrinsic and thalamic excitatory inputs onto songbird LMAN neurons differ in their pharmacological and temporal properties[J]. Journal of Neurophysiology,1998,79(5):2615-2628.
[12] DOUPE A J,KUHL P K. Birdsong and human speech:common themes and mechanisms[J]. Annual Review of Neuroscience,1999,22:567-631.
[13] HERRMANN K,ARNOLD A P. The development of afferent projections to the robust archistriatal nucleus in male zebra finches:a quantitative electron microscopic study[J]. The Journal of Neuroscience:the Official Journal of the Society for Neuroscience,1991,11(7):2063-2074.
[14] KAO M H,DOUPE A J,BRAINARD M S. Contributions of an avian basal ganglia-forebrain circuit to real-time modulation of song[J]. Nature,2005,433(7026):638-643.
[15] LIVINGSTON F S,MOONEY R. Androgens and isolation from adult tutors differentially affect the development of songbird neurons critical to vocal plasticity[J]. Journal of Neurophysiology,2001,85(1):34-42.
[16] SINGH T D,BASHAM M E,NORDEEN E J,et al. Early sensory and hormonal experience modulate age-related changes in NR2B mRNA within a forebrain region controlling avian vocal learning[J]. Journal of Neurobiology,2000,44(1):82-94.
[17] SINGH T D,HEINRICH J E,WISSMAN A M,et al. Seasonal regulation of NMDA receptor NR2B mRNA in the adult canary song system[J]. Journal of Neurobiology,2003,54(4):593-603.