15味蒙药对4种血清型牛源致病性大肠杆菌体外抑菌效果研究
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摘要
为了筛选具有抑制牛源致病性大肠杆菌的蒙药,采用水煎煮方法提取浑钦等15味蒙药的水提液,应用牛津杯法测定15味蒙药的抑菌圈直径,通过微量稀释法和平板法测定最小抑菌浓度(MIC)和最小杀菌浓度(MBC)。结果表明:浑钦和高勒图宝日对4种血清型牛源致病性大肠杆菌抑菌效果较好,均达到极度敏感或高度敏感;这两种蒙药对4种血清型牛源致病性大肠杆菌的MIC为0.016～0.125 g/mL,MBC为0.063～0.5 g/mL。说明蒙药浑钦和高勒图宝日有希望用于治疗大肠杆菌病的药物研发。
In order to screen Mongolian herb medicine for inhibiting pathogenic E.coli from cattle, the water extract of 15 kinds of Mongolian herb medicines such as hunqin was extracted by water decoction. The diameter of inhibition zone was determined by Oxford cup method, micro dilution and plate method were determined by minimal inhibitory concentration(MIC) and minimum bactericidal concentration(MBC).The result showed that hunqin and Gaoletubaori have a good antibacterial effect on pathogenic E.coli of four serotypes, both of which were extremely sensitive or highly sensitive.The MIC of these two Mongolian drugs against the four serotypes of bovine pathogenic E.coli was 0.016-0.125 g/mL, and the MBC was 0.063-0.5 g/mL. It indicates that the Mongolian herb medicine hunqin and Gaoletubaori are potential to be used in the development of drugs for the treatment of E.coli.
In order to screen Mongolian herb medicine for inhibiting pathogenic E.coli from cattle, the water extract of 15 kinds of Mongolian herb medicines such as hunqin was extracted by water decoction. The diameter of inhibition zone was determined by Oxford cup method, micro dilution and plate method were determined by minimal inhibitory concentration(MIC) and minimum bactericidal concentration(MBC).The result showed that hunqin and Gaoletubaori have a good antibacterial effect on pathogenic E.coli of four serotypes, both of which were extremely sensitive or highly sensitive.The MIC of these two Mongolian drugs against the four serotypes of bovine pathogenic E.coli was 0.016-0.125 g/mL, and the MBC was 0.063-0.5 g/mL. It indicates that the Mongolian herb medicine hunqin and Gaoletubaori are potential to be used in the development of drugs for the treatment of E.coli.
引文
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[11] 袁文静.丁香乙醇提取液对临床分离菌株的抗菌作用及毒性试验[J].中国消毒学杂志, 2011, 28(6):689-691.
[12] 丁月云,李茜,王恬. 21种中药对奶牛乳房炎3种病原菌的体外抑菌试验[J].畜牧与兽医,2004,36(12):5-7.
[13] 代如意,李莉,殷中琼,等.5味中药对鸡白痢沙门氏菌的体外联合抑菌研究[J].西北农林科技大学学报,2015,43(2):34-37.
[14] 岳云升,李仲兴,王秀兴,等.用新方法对 308 株临床菌株抗菌活性研究[J].航空航天医药,2004,15(1): 16-18.
[15] 陈希文,王雄清,代敏,等.24种中药对猪沙门氏菌的体外抑菌活性[J].江苏农业科学, 2008(5):180-182,205.
[2] 张楚贞,刘远波,魏灿辉,等.中药水提物及其复方对大肠杆菌的体外抑菌效果[J].中兽医医药杂志,2014(4):17-20.
[3] 易勇. EHEC O157:H7紧密粘附素胞外区的晶体结构及功能研究[D].重庆:第三军医大学,2007.
[4] BARRINGTON G M,GAY J M, EVERMANN J F. Biosecuritu for neonatal gastrointestinal diseases [J]. Vet Clin North Am Food Anim Pract, 2002,18(1): 7-34.
[5] 徐成刚,任涛,廖明,等.兽用抗生素对公共卫生的影响[J].动物医学进展,2002,6(14):45-48.
[6] 杨华,许冰白.畜禽产品中药物残留监控的进展及重要要意义[J].兽药与饲料添加剂,2001,6(4):39-41.
[7] 刘荣欣,鲁改儒,郭吉勇.中药及其组方对大肠杆菌的体外抑菌作用试验[J].安徽农业科学,2011,39(4):2265-2267.
[8] 斯木吉德,敖日格乐,王纯洁,等. 6种血清型牛源致病性大肠杆菌的体外抑菌效果研究[J].黑龙江畜牧兽医,2009(10上):69-70
[9] 刘冬梅,李理,杨晓泉,等.用牛津杯法测定益生菌的抑菌活力[J].食品研究与开发,2006,27(3):110-111.
[10] 杨斯琴,敖日格乐,王纯洁,等.蒙药对牛源致病性大肠杆菌的体外抑菌效果研究[J].中国农业大学学报,2015,20(1):124-128.
[11] 袁文静.丁香乙醇提取液对临床分离菌株的抗菌作用及毒性试验[J].中国消毒学杂志, 2011, 28(6):689-691.
[12] 丁月云,李茜,王恬. 21种中药对奶牛乳房炎3种病原菌的体外抑菌试验[J].畜牧与兽医,2004,36(12):5-7.
[13] 代如意,李莉,殷中琼,等.5味中药对鸡白痢沙门氏菌的体外联合抑菌研究[J].西北农林科技大学学报,2015,43(2):34-37.
[14] 岳云升,李仲兴,王秀兴,等.用新方法对 308 株临床菌株抗菌活性研究[J].航空航天医药,2004,15(1): 16-18.
[15] 陈希文,王雄清,代敏,等.24种中药对猪沙门氏菌的体外抑菌活性[J].江苏农业科学, 2008(5):180-182,205.