气相色谱-质谱法检测黑面神对大鼠肝肾机体代谢的影响
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摘要
目的通过采用气相色谱-质谱法(GC-MS)结合代谢组学方法以阐明南方"傣族"少数民族治疗慢性支气管炎和炎症的民间药物黑面神(大戟科)对大鼠肝肾机体代谢的影响。方法将大鼠分为对照组、黑面神(2 g/kg)组和10%酒精处理组,连续给药7 d。第8 d,从3组大鼠收集肝脏和肾脏样本,衍生化处理后,进行GC-MS分析。结果根据肝脏和肾脏的代谢组学偏最小二乘法鉴别分析(PLS-DA),结果显示黑面神组与对照组和10%酒精处理组区别开来。在肝组织中,黑面神组与对照组之间没有发现差异,而黑面神组与10%乙醇处理组之间的核糖醇和D-葡萄糖含量差异有统计学意义(P<0.05)。在肾组织中,黑面神组相对10%酒精处理组,硬脂酸含量升高(P<0.05)。结论 PLS-DA结果表明,黑面神诱导代谢扰动,而10%的酒精也可能诱发代谢扰动。
Objective Gas chromatography-mass spectrometry( GC-MS) coupled with metabolomic method was used to elucidate the effect of Breynia fruticosa( Euphorbiaceae),a folk medicine for the treatment of chronic bronchitis and inflammation by Dai in southern China,on liver and kidney of rats. Methods The rats were divided into the control group,Breynia fruticosa( 2 g/kg) treated group,10% alcohol treated group,and administrated continuously for 7 d. On the 8 thday,liver and kidney of the rats were collected from three groups and the extracts were derivatized and analyzed by GC-MS. Results According to the liver and kidney metabolomics results,Breynia fruticosa treated rats could be distinguished from control group and 10% alcohol treated group. In the liver samples,there is no difference between Breynia fruticosa treat group and control group,while the content of ribitol and D-glucose had significant difference between Breynia fruticosa treat group and 10% alcohol treated group( P < 0. 05). In the kidney samples,octadecanoic acid increased in Breynia fruticosa treated group compared to 10% alcohol treated group( P < 0. 05). Conclusion Partial least squares-discriminate analysis( PLS-DA) revealed that Breynia fruticosa induced metabolic perturbations,while the 10% alcohol could also induce metabolic perturbations.
Objective Gas chromatography-mass spectrometry( GC-MS) coupled with metabolomic method was used to elucidate the effect of Breynia fruticosa( Euphorbiaceae),a folk medicine for the treatment of chronic bronchitis and inflammation by Dai in southern China,on liver and kidney of rats. Methods The rats were divided into the control group,Breynia fruticosa( 2 g/kg) treated group,10% alcohol treated group,and administrated continuously for 7 d. On the 8 thday,liver and kidney of the rats were collected from three groups and the extracts were derivatized and analyzed by GC-MS. Results According to the liver and kidney metabolomics results,Breynia fruticosa treated rats could be distinguished from control group and 10% alcohol treated group. In the liver samples,there is no difference between Breynia fruticosa treat group and control group,while the content of ribitol and D-glucose had significant difference between Breynia fruticosa treat group and 10% alcohol treated group( P < 0. 05). In the kidney samples,octadecanoic acid increased in Breynia fruticosa treated group compared to 10% alcohol treated group( P < 0. 05). Conclusion Partial least squares-discriminate analysis( PLS-DA) revealed that Breynia fruticosa induced metabolic perturbations,while the 10% alcohol could also induce metabolic perturbations.
引文
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[2]霍培元,陈华红,姜怡,等.傣药黑面神内生菌Bacillus pumilus化学成分研究[J].中国药物化学杂志,2012,22(1):38-43.
[3]彭伟文,戴卫波,梅全喜,等.黑面神不同极性部位HPLC色谱与抗慢性皮炎-湿疹疗效的关联性研究[J].亚太传统医药,2016,12(14):41-45.
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[8]郑隽,王永红,王继红,等.重庆市9528例体检人群脂代谢特点及脂代谢异常的临床研究[J].中国卫生检验杂志,2007,17(2):299-301.
[9]王晶,汤柳英,杨杏芬,等.代谢组学技术及其研究进展[J].中国卫生检验杂志,2013,23(4):1046-1050.
[10]王根芬.妊娠期肝内胆汁淤积症人群血清脂代谢与肝功能相关指标的临床分析[J].中国卫生检验杂志,2015,25(11):1778-1780.
[11]汤柳英,高燕红,王晶,等.不同储存条件下人尿中小分子代谢物表征差异的初步研究[J].中国卫生检验杂志,2014,24(5):612-617.
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[13]孙玉萍,苗蕾,张蓓,等.尿酸与糖脂代谢指标的关联性及民族异质性研究[J].中国卫生检验杂志,2014,24(4):595-598.
[14]蒋晓颖,金晶.血清黄嘌呤氧化酶在代谢综合征患者中的意义[J].中国卫生检验杂志,2013,23(13):2778-2780.
[15]蒋武斌,陈柏康,孙良学.上尿路结石与血脂代谢异常及炎症的关系研究[J].中国卫生检验杂志,2017,27(18):2633-2635.
[16]郭宾,戴仁科.代谢组学及其研究策略和分析方法进展[J].中国卫生检验杂志,2007,17(3):554-563.
[17]陈孟权,周永列,余细平,等.骨代谢标志物组合模型对诊断肺癌骨转移的价值[J].中国卫生检验杂志,2016,26(18):2648-2651.
[18]纪勇,郭盛磊,杨玉焕.代谢组学方法研究进展[J].安徽农业科学,2015,43(25):21-23.
[19]任向楠,梁琼麟.基于质谱分析的代谢组学研究进展[J].分析测试学报,2017,36(2):161-169.
[2]霍培元,陈华红,姜怡,等.傣药黑面神内生菌Bacillus pumilus化学成分研究[J].中国药物化学杂志,2012,22(1):38-43.
[3]彭伟文,戴卫波,梅全喜,等.黑面神不同极性部位HPLC色谱与抗慢性皮炎-湿疹疗效的关联性研究[J].亚太传统医药,2016,12(14):41-45.
[4]彭伟文,王英晶,陆丹倩,等.黑面神茎、叶不同提取物抑菌作用对比研究[J].中国医院药学杂志,2014,34(11):869-873.
[5]彭伟文,王英晶,陆丹倩,等.黑面神枝叶水提物抑菌有效部位的筛选研究[J].中华中医药学刊,2014,32(12):2937-2939.
[6]彭伟文,王英晶.黑面神的药用历史及现代研究概况[J].今日药学,2014,24(8):618-622.
[7]林理根,柯昌强,叶阳.黑面神根部化学成分的研究[J].中草药,2013,44(22):3119-3122.
[8]郑隽,王永红,王继红,等.重庆市9528例体检人群脂代谢特点及脂代谢异常的临床研究[J].中国卫生检验杂志,2007,17(2):299-301.
[9]王晶,汤柳英,杨杏芬,等.代谢组学技术及其研究进展[J].中国卫生检验杂志,2013,23(4):1046-1050.
[10]王根芬.妊娠期肝内胆汁淤积症人群血清脂代谢与肝功能相关指标的临床分析[J].中国卫生检验杂志,2015,25(11):1778-1780.
[11]汤柳英,高燕红,王晶,等.不同储存条件下人尿中小分子代谢物表征差异的初步研究[J].中国卫生检验杂志,2014,24(5):612-617.
[12]Dinis-Oliveira RJ.Metabolomics of drugs of abuse:a more realistic view of the toxicological complexity[J].Bioanalysis,2014,6(23):3155-3159.
[13]孙玉萍,苗蕾,张蓓,等.尿酸与糖脂代谢指标的关联性及民族异质性研究[J].中国卫生检验杂志,2014,24(4):595-598.
[14]蒋晓颖,金晶.血清黄嘌呤氧化酶在代谢综合征患者中的意义[J].中国卫生检验杂志,2013,23(13):2778-2780.
[15]蒋武斌,陈柏康,孙良学.上尿路结石与血脂代谢异常及炎症的关系研究[J].中国卫生检验杂志,2017,27(18):2633-2635.
[16]郭宾,戴仁科.代谢组学及其研究策略和分析方法进展[J].中国卫生检验杂志,2007,17(3):554-563.
[17]陈孟权,周永列,余细平,等.骨代谢标志物组合模型对诊断肺癌骨转移的价值[J].中国卫生检验杂志,2016,26(18):2648-2651.
[18]纪勇,郭盛磊,杨玉焕.代谢组学方法研究进展[J].安徽农业科学,2015,43(25):21-23.
[19]任向楠,梁琼麟.基于质谱分析的代谢组学研究进展[J].分析测试学报,2017,36(2):161-169.