3种富硒菊花的化学成分及其对α-葡萄糖苷酶活力的影响
|
摘要
为了探究富硒菊花的食用安全性与营养保健功效,给富硒菊花种质资源选育和开发利用提供依据。本研究采用全自动氨基酸分析仪等分析了金丝菊、香菊、雪菊3种富硒菊花的营养组成,采用高效液相色谱法测定了9种酚类、黄酮类化合物的含量,采用原子荧光光谱法等测定了重金属含量,分析了富硒菊花的抗氧化活性及其对α-葡萄糖苷酶的抑制效应。结果发现,3种菊花的硒元素含量在0.125 9～0.195 4μg/g之间。雪菊的水分、灰分含量最高,金丝菊的粗脂肪含量最高,香菊的总蛋白、清蛋白和球蛋白含量最高,同时具有较高的必需氨基酸化学评分和氨基酸评分,说明香菊的氨基酸组成与人体需求较为接近。3种菊花均含有3-咖啡酰奎宁酸、4,5-二咖啡酰奎宁酸、木犀草苷、芹菜苷、蒙花苷、木犀草素、芹菜素、香叶木素和金合欢素,均未检出镉与汞,铜、砷和铅含量分别在13.250～20.875μg/g、0.000～0.087μg/g、0.375～1.038μg/g之间。富硒菊花具有一定的总还原力与DPPH·、ABTS·+、·OH自由基清除活性,能够抑制α-葡萄糖苷酶的活力。本研究为富硒菊花种质资源选育和开发利用提供了参考依据。
To study safety, nutrition and bioactivity of Se-enriched Compositae flowers and provide basis for their breeding, exploration and utilization, nutritional composition of three kinds of Compositae flowers, including Chrysanthemum morifolium cv. 'Jinsiju', Chrysanthemum morifolium cv. 'Xiangju' and Coreopsis tinctoria, were investigated by automatic amino acid analyzer. Nine phenols and flavonoids in three kinds of flowers were quantified by high-performance liquid chromatography. Contents of heavy metals were determined by atomic absorption spectrometry and atomic fluorescence spectrometry. Besides, antioxidant activities and inhibiting effect of three kinds of flowers on α-glucosidase were analyzed. The results showed that selenium content in three kinds of flowers ranged from 0.125 9 to 0.195 4 mg/kg. Coreopsis tinctoria had the highest moisture and ash contents, while the highest crude fat content was found in C. morifolium cv. 'Jinsiju'. C. morifolium cv. 'Xiangju' had the highest total protein content, albumin content and globulin content. In addition, it had high essential amino acid chemical score and amino acid score, indicating that amino acid composition of C. morifolium cv. 'Xiangju' basically met amino acid requirements in human nutrition. Three kinds of Se-enriched Compositae flowers contained 3-caffeoylquinic acid,4,5-dibuteoylquinic acid, luteolin, apigenin, linarin, luteolin, apigenin, diosmin, and farnesin. Cd and Hg were not detected in three kinds of flowers, and contents of Cu, As and Pb were in the range of 13.250～20.875 μg/g,0.000～0.087 μg/g and 0.375～1.038 μg/g, respectively. Se-enriched Compositae flowers possessed certain total reducing power and DPPH·, ABTS·+and ·OH radical scavenging activity, which could inhibit the activity ofα-glucosidase. This study could provide reference for breeding, exploration and utilization of Se-enriched Compositae flowers.
To study safety, nutrition and bioactivity of Se-enriched Compositae flowers and provide basis for their breeding, exploration and utilization, nutritional composition of three kinds of Compositae flowers, including Chrysanthemum morifolium cv. 'Jinsiju', Chrysanthemum morifolium cv. 'Xiangju' and Coreopsis tinctoria, were investigated by automatic amino acid analyzer. Nine phenols and flavonoids in three kinds of flowers were quantified by high-performance liquid chromatography. Contents of heavy metals were determined by atomic absorption spectrometry and atomic fluorescence spectrometry. Besides, antioxidant activities and inhibiting effect of three kinds of flowers on α-glucosidase were analyzed. The results showed that selenium content in three kinds of flowers ranged from 0.125 9 to 0.195 4 mg/kg. Coreopsis tinctoria had the highest moisture and ash contents, while the highest crude fat content was found in C. morifolium cv. 'Jinsiju'. C. morifolium cv. 'Xiangju' had the highest total protein content, albumin content and globulin content. In addition, it had high essential amino acid chemical score and amino acid score, indicating that amino acid composition of C. morifolium cv. 'Xiangju' basically met amino acid requirements in human nutrition. Three kinds of Se-enriched Compositae flowers contained 3-caffeoylquinic acid,4,5-dibuteoylquinic acid, luteolin, apigenin, linarin, luteolin, apigenin, diosmin, and farnesin. Cd and Hg were not detected in three kinds of flowers, and contents of Cu, As and Pb were in the range of 13.250～20.875 μg/g,0.000～0.087 μg/g and 0.375～1.038 μg/g, respectively. Se-enriched Compositae flowers possessed certain total reducing power and DPPH·, ABTS·+and ·OH radical scavenging activity, which could inhibit the activity ofα-glucosidase. This study could provide reference for breeding, exploration and utilization of Se-enriched Compositae flowers.
引文
Adebiyi A.P.,Adebiyi A.O.,Hasegsawa Y.,Ogawa T.,and Muramoto K.,2009,Isolation and characterization of protein fractions from deoiled rice bran,Eur.Food Res.Technol.,228(3):391-401
Adler E.M.,2006,Focus issue:exciting times for signaling in the brain,Sci.Signal.,356(11):1-3
Debnath T.,Jin H.L.,Hasnat M.A.,Kim Y.,Samad N.B.,and Park P.J.,2013,Antioxidant potential and oxidative DNAdamage preventive activity of chrysanthemum indicum extracts,J.Food Biochem.,37(4):440-448
Han A.R.,Kim H.Y.,So Y.,Nam B.,Lee I.S.,and Nam J.W.,2017,Quantification of antioxidant phenolic compounds in a new chrysanthemum cultivar by high-performance liquid chromatography with diode array detection and electr ospray ionization mass spectrometry,Int.J.Anal.Chem.,doi:10.1155/2017/1254721
He K.,Shi J.C.,and Mao X.M.,2014,Safety and efficacy of acarbose in the treatment of diabetes in Chinese patients,T-her.Clin.Risk Manag.,10:505-511
Jan F.A.,Ishaq M.,Khan S.,Shakirullah M.,Asim S.M.,and Ahmad I.,2011,Bioaccumulation of metals in human blood in industrially contaminated area,J.Environ.Sci.,23(12):2069-2077
Kápolna E.,and Fodor P.,2007,Bioavailability of selenium from selenium-enriched green onions(Allium fistulosum)and chives(Allium schoenoprasum)after'in vitro'gastrointestinal digestion,Int.J.Food.Sci.Nutr.,58(4):282-296
Kazuya I.,Noriaki K.,Yukari K.,Kyo M.A.,and Fujita T.,2004,In vitro antioxidative effects and tyrosinase inhibitory activities of seven hydroxycinnamoyl derivatives in green coffee beans,J.Agr.Food Chem.,52(15):4893-4898
Lee W.K.,Wong L.L.,Loo Y.Y.,Kasapis S.,and Huang D.,2010,Evaluation of different teas against starch digestibility by mammalian glycosidases,J.Agr.Food Chem.,58(1):148-154
Molan A.L.,Flanagan J.,Wei W.,and Moughan P.J.,2009,Selenium-containing green tea has higher antioxidant and prebiotic activities than regular green tea,Food Chem.,114(6):829-835
Rayman M.P.,2000,The importance of selenium to human health,Lancet,356(9225):233-241
Reamer D.C.,and Zoller W.H.,1980,Selenium biomethylation products from soil and sewage sludge,Sci.,208(4443):500-502
Rodríguez G.B.,Rios M.D.,Rodríguez R.E.M.,and Romero C.D.,2010,Amino acid content in traditional potato cultivars from the Canary Islands,J.Food Compos.Anal.,23(2):148-153
Spallholz J.E.,1994,On the nature of selenium toxicity and carcinostatic activity,Free Radical Biol.Med.,17(1):45-64
Tan Y.,Chang S.,and Zhang Y.,2017,Comparison of a-amylase,a-glucosidase and lipase inhibitory activity of the phenolic substances in two black legumes of different genera,Food Chem.,214:259-268
Tseng Y.H.,Yang J.H.,and Mau J.L.,2008,Antioxidant properties of polysaccharides from Ganoderma tsugae,Food Chem.,107(2):732-738
Van Dijk A.E.,Olthof M.R.,Meeuse J.C.,Seebus E.,Heine R.J.,and Dam R.M.V.,2009,Acute effects of decaffeinated coffee and the major coffee components chlorogenic acid and trigonelline on glucose tolerance,Diabetes Care,32(6):1023-1025
Van De Laar F.A.,Lucassen P.L.,Akkermans R.P.,Van De Lisdonk E.H.,Rutten G.E.,and Van Weel C.,2005,α-glucosidase inhibitors for patients results from a cochrane systematic review and meta-analysis,Diabetes Care,28(1):166-175
Wu G.,Bazer F.W.,Davis T.A.,Kim S.W.,Li P.,and Rhoads J.M.,2009,Arginine metabolism and nutrition in growth,health and disease,Amino Acids,37(1):153-168
Yang Y.J.,Liu X.H.,Ning C.J.,Ji A.Q.,Ding N.,and Xu L.J.,2013,Effects of foliar feeding of selenium on fruit quality and accumulation of cadmium,lead and mercury in sweet persimmon,Yuanyi Xuebao(Acta Horticulturae Sinica),40(3):523-530(杨燕君,刘晓华,宁婵娟,冀爱青,丁宁,许领军,2013,叶面施硒对甜柿果实品质及重金属含量的影响,园艺学报,40(3):523-530)
Yang X.H.,Zhang H.F.,Niu L.L.,Wang Y.,and Lai J.H.,2017,Contents of heavy metals in Chinese edible herbs:evidence from a case study of Epimedii Folium,Biol.Trace Elem.Res.,182(1):1-10
Young V.R.,and Ajami A.M.,2000,Glutamate:an amino acid of particular distinction,J.Nure.,130(4S):892-900
Yu Z.L.,Gao H.X.,Zhang Z.,He Z.,He Q.,and Jia L.R.,2015,Inhibitory effects of Ligustrum robustum(Rxob.)Blume extract onα-amylase andα-glucosidase,J.Funct.Foods,19(A):204-213
Zhang H.F.,Zhang X.,Yang X.H.,Qiu N.X.,Wang Y.,and Wang Z.Z.,2013,Microwave assisted extraction of flavonoids from cultivated Epimedium sagittatum:extraction yield and mechanism,antioxidant activity and chemical composition,Ind.Crop.Prod.,50(10):857-865
Zhao Z.H.,Zhang F.R.,Han Y.X.,Li Q.,Zhang H.F.,and Wang X.Y.,2018,Antioxidant activities and effects onα-amylase andα-glucosidase of extract from Se-enriched Chrysanthemum morifolium,Tianran Chanwu Yanjiu Yu Kaifa(Natural Product Research and Development),30(7):1105-1110(赵子华,张斐然,韩永旭,李巧,张华峰,王星宇,2018,富硒菊花提取物的抗氧化活性及其对α-淀粉酶和α-葡萄糖苷酶活力的影响,天然产物研究与开发,30(7):1105-1110)
Zheng C.,Dong Q.,Chen H.,Cong Q.,and Ding K.,2015,Structural characterization of a polysaccharide from Chrysanthemum morifolium flowers and its antioxidant activity,Carbohyd.Polym.,130(4):113-121
Adler E.M.,2006,Focus issue:exciting times for signaling in the brain,Sci.Signal.,356(11):1-3
Debnath T.,Jin H.L.,Hasnat M.A.,Kim Y.,Samad N.B.,and Park P.J.,2013,Antioxidant potential and oxidative DNAdamage preventive activity of chrysanthemum indicum extracts,J.Food Biochem.,37(4):440-448
Han A.R.,Kim H.Y.,So Y.,Nam B.,Lee I.S.,and Nam J.W.,2017,Quantification of antioxidant phenolic compounds in a new chrysanthemum cultivar by high-performance liquid chromatography with diode array detection and electr ospray ionization mass spectrometry,Int.J.Anal.Chem.,doi:10.1155/2017/1254721
He K.,Shi J.C.,and Mao X.M.,2014,Safety and efficacy of acarbose in the treatment of diabetes in Chinese patients,T-her.Clin.Risk Manag.,10:505-511
Jan F.A.,Ishaq M.,Khan S.,Shakirullah M.,Asim S.M.,and Ahmad I.,2011,Bioaccumulation of metals in human blood in industrially contaminated area,J.Environ.Sci.,23(12):2069-2077
Kápolna E.,and Fodor P.,2007,Bioavailability of selenium from selenium-enriched green onions(Allium fistulosum)and chives(Allium schoenoprasum)after'in vitro'gastrointestinal digestion,Int.J.Food.Sci.Nutr.,58(4):282-296
Kazuya I.,Noriaki K.,Yukari K.,Kyo M.A.,and Fujita T.,2004,In vitro antioxidative effects and tyrosinase inhibitory activities of seven hydroxycinnamoyl derivatives in green coffee beans,J.Agr.Food Chem.,52(15):4893-4898
Lee W.K.,Wong L.L.,Loo Y.Y.,Kasapis S.,and Huang D.,2010,Evaluation of different teas against starch digestibility by mammalian glycosidases,J.Agr.Food Chem.,58(1):148-154
Molan A.L.,Flanagan J.,Wei W.,and Moughan P.J.,2009,Selenium-containing green tea has higher antioxidant and prebiotic activities than regular green tea,Food Chem.,114(6):829-835
Rayman M.P.,2000,The importance of selenium to human health,Lancet,356(9225):233-241
Reamer D.C.,and Zoller W.H.,1980,Selenium biomethylation products from soil and sewage sludge,Sci.,208(4443):500-502
Rodríguez G.B.,Rios M.D.,Rodríguez R.E.M.,and Romero C.D.,2010,Amino acid content in traditional potato cultivars from the Canary Islands,J.Food Compos.Anal.,23(2):148-153
Spallholz J.E.,1994,On the nature of selenium toxicity and carcinostatic activity,Free Radical Biol.Med.,17(1):45-64
Tan Y.,Chang S.,and Zhang Y.,2017,Comparison of a-amylase,a-glucosidase and lipase inhibitory activity of the phenolic substances in two black legumes of different genera,Food Chem.,214:259-268
Tseng Y.H.,Yang J.H.,and Mau J.L.,2008,Antioxidant properties of polysaccharides from Ganoderma tsugae,Food Chem.,107(2):732-738
Van Dijk A.E.,Olthof M.R.,Meeuse J.C.,Seebus E.,Heine R.J.,and Dam R.M.V.,2009,Acute effects of decaffeinated coffee and the major coffee components chlorogenic acid and trigonelline on glucose tolerance,Diabetes Care,32(6):1023-1025
Van De Laar F.A.,Lucassen P.L.,Akkermans R.P.,Van De Lisdonk E.H.,Rutten G.E.,and Van Weel C.,2005,α-glucosidase inhibitors for patients results from a cochrane systematic review and meta-analysis,Diabetes Care,28(1):166-175
Wu G.,Bazer F.W.,Davis T.A.,Kim S.W.,Li P.,and Rhoads J.M.,2009,Arginine metabolism and nutrition in growth,health and disease,Amino Acids,37(1):153-168
Yang Y.J.,Liu X.H.,Ning C.J.,Ji A.Q.,Ding N.,and Xu L.J.,2013,Effects of foliar feeding of selenium on fruit quality and accumulation of cadmium,lead and mercury in sweet persimmon,Yuanyi Xuebao(Acta Horticulturae Sinica),40(3):523-530(杨燕君,刘晓华,宁婵娟,冀爱青,丁宁,许领军,2013,叶面施硒对甜柿果实品质及重金属含量的影响,园艺学报,40(3):523-530)
Yang X.H.,Zhang H.F.,Niu L.L.,Wang Y.,and Lai J.H.,2017,Contents of heavy metals in Chinese edible herbs:evidence from a case study of Epimedii Folium,Biol.Trace Elem.Res.,182(1):1-10
Young V.R.,and Ajami A.M.,2000,Glutamate:an amino acid of particular distinction,J.Nure.,130(4S):892-900
Yu Z.L.,Gao H.X.,Zhang Z.,He Z.,He Q.,and Jia L.R.,2015,Inhibitory effects of Ligustrum robustum(Rxob.)Blume extract onα-amylase andα-glucosidase,J.Funct.Foods,19(A):204-213
Zhang H.F.,Zhang X.,Yang X.H.,Qiu N.X.,Wang Y.,and Wang Z.Z.,2013,Microwave assisted extraction of flavonoids from cultivated Epimedium sagittatum:extraction yield and mechanism,antioxidant activity and chemical composition,Ind.Crop.Prod.,50(10):857-865
Zhao Z.H.,Zhang F.R.,Han Y.X.,Li Q.,Zhang H.F.,and Wang X.Y.,2018,Antioxidant activities and effects onα-amylase andα-glucosidase of extract from Se-enriched Chrysanthemum morifolium,Tianran Chanwu Yanjiu Yu Kaifa(Natural Product Research and Development),30(7):1105-1110(赵子华,张斐然,韩永旭,李巧,张华峰,王星宇,2018,富硒菊花提取物的抗氧化活性及其对α-淀粉酶和α-葡萄糖苷酶活力的影响,天然产物研究与开发,30(7):1105-1110)
Zheng C.,Dong Q.,Chen H.,Cong Q.,and Ding K.,2015,Structural characterization of a polysaccharide from Chrysanthemum morifolium flowers and its antioxidant activity,Carbohyd.Polym.,130(4):113-121