纳豆激酶体外溶栓作用研究
摘要
目的:以初凝的小鼠血块为样本模拟小鼠血栓,研究纳豆激酶不同浓度对小鼠的溶栓作用,从而得到溶栓效果好且不溶血的最佳纳豆激酶浓度。方法:采用天然界筛选的菌种发酵提取的2个批次的纳豆激酶NK-1、NK-2,分别稀释至1 000、500、250、125、62.5、31.25和15.63U/mL溶解小鼠体外血凝块,以尿激酶为对照,观测计算0～7h内血栓溶解率,并用显微镜及试管观察0～18h内溶栓与溶血效果。结果:500、1 000U/mL纳豆激酶在7h内能完全溶解0.12g左右的小鼠体外血凝块,125、250、500、1000U/mL的纳豆激酶溶解血凝块作用依次增强,表现出一定的剂量效应,尤其是发现125～250U/mL纳豆激酶体外溶栓效果较好,且溶血作用不明显,且500～1000U/mL纳豆激酶可能存在溶血作用。结论:该研究结果可为纳豆激酶新型药物的开发提供理论依据,而且具有一定的产业化参考意义。
引文
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[3]余巧燕,孙晋民.溶栓药物的研究进展[J].医学理论与实践,2009,22(1):30-33.
[4]高泽鑫,何腊平,刘亚兵,等.纳豆激酶的研究进展与展望[J].中国酿造,2017,36(8):11-15.
[5]任武贤,王星星,戴东升.纳豆激酶液态发酵工艺的优化[J].大豆科学,2015,34(1):000128-130.
[6]毛娜娜,谢梅林,顾振纶,等.纳豆激酶溶栓作用研究进展[J].中国血液流变学杂志,2008,18(2):306-309.
[7]李静,宋艳志,王梦静,等.纳豆激酶与尿激酶的对比性研究[J].中国药剂学杂志:网络版,2016(4):125-134.
[8]SUMI H,HAMADA H,NAKANISHI K,et al.Enhancement of the fibrinolytic activity in plasma by oral administration of nattokinase[J].Acta Haematologica,1990,84(3):139-143.
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