甘蓝黑腐病和枯萎病兼抗材料的鉴定筛选
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摘要
采用喷雾法与浸根法,分别对99份国内外甘蓝种质资源(83份自交系、16份杂交种)进行黑腐病与枯萎病苗期人工接种抗性鉴定。共筛选出26份抗黑腐病的材料,其中2份表现高抗,即99-192和20-2-5(病情指数<10);同时还筛选获得引162、奥奇娜、96-100-919等54份高抗枯萎病的材料(病情指数<10)。其中,秋德、99-192、HB34、JS119、ZL66为高抗或抗黑腐病兼高抗枯萎病的材料,且JS119和ZL66为早熟、圆球类型材料。进一步对抗性材料的地理来源及主要农艺性状进行分析,发现83份自交系材料中高抗枯萎病的材料多来自日、韩等国家,且多为早熟、圆球类型;而高抗黑腐病材料多为晚熟、扁球类型,早熟类型中对黑腐病有较强抗性的材料较少。分别对不同抗性材料配制的16份杂交种进行遗传分析,结果表明甘蓝黑腐病、枯萎病的抗性分别符合隐性和显性遗传。
By spraying and root-dipping methods,this study carried out identification of resistance to black rot and Fusarium wilt on 99 cabbage accessions from domestic and overseas,including 83 inbred lines and 16 hybrid combinations.In total,26 accessions showed resistance to black rot,of which‘99-192'and‘20-2-5' with high resistance(DI < 10);54 accessions with high resistance to Fusarium wilt,including ‘Yin162',‘Aoqina'and‘96-100-919'(DI < 10).Among them,5 accessions with high resistance or resistance to black rot+high resistance to Fusarium wilt.They were‘Qiude',‘99-192',‘HB34',‘JS119'and‘ZL66'. ‘JS119'and‘ZL66'were of early-maturity and round ball type.This study also conducted further analysis on the geographical origin and major agronomic traits of these resistant materials.The results showed that accessions highly resistant to Fusarium wilt out of 83 inbred lines were mostly originated from Japan and Korea and of earlymaturity and round ball type.While,accessions highly resistant to black rot were mostly of late maturity and spheroidicity.Among early maturing material,there were few accessions with stronger resistance to black rot.Genetic analysis on 16 hybrid combinations made up by different resistance showed that black rot and Fusarium wilt resistance was accorded with recessive and dominant inheritance,respectively.
By spraying and root-dipping methods,this study carried out identification of resistance to black rot and Fusarium wilt on 99 cabbage accessions from domestic and overseas,including 83 inbred lines and 16 hybrid combinations.In total,26 accessions showed resistance to black rot,of which‘99-192'and‘20-2-5' with high resistance(DI < 10);54 accessions with high resistance to Fusarium wilt,including ‘Yin162',‘Aoqina'and‘96-100-919'(DI < 10).Among them,5 accessions with high resistance or resistance to black rot+high resistance to Fusarium wilt.They were‘Qiude',‘99-192',‘HB34',‘JS119'and‘ZL66'. ‘JS119'and‘ZL66'were of early-maturity and round ball type.This study also conducted further analysis on the geographical origin and major agronomic traits of these resistant materials.The results showed that accessions highly resistant to Fusarium wilt out of 83 inbred lines were mostly originated from Japan and Korea and of earlymaturity and round ball type.While,accessions highly resistant to black rot were mostly of late maturity and spheroidicity.Among early maturing material,there were few accessions with stronger resistance to black rot.Genetic analysis on 16 hybrid combinations made up by different resistance showed that black rot and Fusarium wilt resistance was accorded with recessive and dominant inheritance,respectively.
引文
崔瑞峰.2004.甘蓝黑腐病苗期抗病性鉴定研究[硕士论文].晋中:山西农业大学.
方智远.2008.甘蓝栽培技术.北京:金盾出版社:1-7.
方智远.2017.中国蔬菜育种学.北京:中国农业出版社:542-547.
耿丽华,迟胜起,焦晓辉,康俊根,田仁鹏.2009.北京延庆县甘蓝枯萎病病原菌的分离及其生物学特性的研究.中国蔬菜,(2):34-37.
龚静,朱玉英,吴晓光.2001.甘蓝黑腐病抗性材料筛选及接种方法的研究.上海农业科技,(4):87.
黄德芬,李成琼,司军,任雪松,宋洪元.2011.甘蓝黑腐病生理小种划分及其抗病性鉴定研究进展.中国蔬菜,(18):6-10.
简元才,钉贯靖久.1994.甘蓝黑腐病抗病性材料的鉴定及筛选.农业新技术,(3):29-30.
康俊根,田仁鹏,耿丽华,陈延阳,简元才,丁云花.2010.甘蓝抗枯萎病种质资源的筛选及抗性基因分布频率分析.中国蔬菜,(2):15-20.
李明远.1997.注意防治十字花科黑腐病.蔬菜,(4):23-24.
李明远,张涛涛,李兴红,严红.2003.十字花科蔬菜枯萎病及其病原鉴定.植物保护,29(6):44-45.
李明远,马永军,姚金亮.2006.警惕十字花科蔬菜枯萎病在我国蔓延.中国植保导刊,26(1):21-22.
李树德.1995.中国主要蔬菜抗病育种进展.北京:科学出版社:617-619.
李锡香.2008.萝卜种质资源描述规范和数据标准.北京:中国农业出版社:82-83.
李永镐,徐丽波.1990.甘蓝黑腐病苗期抗病性鉴定方法的研究.东北农业大学学报,21(2):125-129.
吕红豪,方智远,杨丽梅,谢丙炎,刘玉梅,庄木,张扬勇,杨宇红.2011.甘蓝枯萎病抗源材料筛选及抗性遗传研究.园艺学报,38(5):875-885.
刘佳,冯兰香,蔡少华,方智远,孙培田.1988.结球甘蓝对Tu MV和黑腐病的抗性鉴定.植物保护,14(6):9-11.
彭锐,雷建军.1998.甘蓝抗黑腐病研究现状.西南园艺,(3):29-32.
田仁鹏,康俊根,耿丽华,颉建明,简元才,丁云花.2009.甘蓝枯萎病抗性鉴定方法研究.中国农学通报,25(4):39-42.
王晓武.1992.甘蓝黑腐病抗性遗传分析[硕士论文].北京:中国农业科学院.
杨丽梅,方智远,庄木,张扬勇,吕红豪,刘玉梅,李占省.2016.“十二五”我国甘蓝遗传育种研究进展.中国蔬菜,(11):1-6.
杨宇红,吕红豪,杨翠荣,龚慧芝,杨丽梅,谢丙炎.2011.甘蓝枯萎病苗期抗性鉴定技术及抗源筛选.植物保护学报,38(5):425-431.
张恩慧,程永安,许忠民,王妍妮,马青山.2001.甘蓝3种病害抗源筛选及抗病品种选育研究.西北农林科技大学学报:自然科学版,29(6):30-33.
张黎黎,刘玉梅,田自华,方智远,杨丽梅,庄木,张扬勇,李占省,舒金帅.2012.十字花科蔬菜抗黑腐病育种研究进展.园艺学报,39(9):1727-1738.
张扬,郑建秋,吴学宏,师迎春,谷培云,李健强.2007.北京延庆甘蓝枯萎病发生和危害调查.中国农学通报,23(5):315-320.
张扬,李金萍,周慧敏,李宝聚.2011.十字花科蔬菜细菌性黑腐病的发生规律及防治.中国蔬菜,(17):23-25.
张云霞.2014.甘蓝苗期黑腐病抗性鉴定及抗病相关机理的研究[硕士论文].南京:南京农业大学.
Alvarez A M.2000.Black rot of crucifers.The Netherlands:Kluwer Aczdemic:21-52.
Bain D C.1952.Reaction of brassica seedlings to black rot.Phytopathology,42:497-500.
Bain D C.1955.Resistance of cabbage to black rot.Phytopathology,45:35-37.
Dickson M D,Hunter J E.1987.Inheritance of resistance in cabbage seedlings to black rot.Hortscience,22(1):108-109.
Guo H,Dickson M H,Hunter J E.1991.Brassica napus sources of resistance to black rot in crucifers and inheritance of resistance.Hortscience,26(12):1545-1547.
Hunter J E,Dickson M H,Ludwig J W.1987.Source of resistance to black rot of cabbage expressed in seedlings and adult plants.Plant Disease,71(3):263-266.
Jensen B D,Vicente J G,Manandhar H K,Roberts S J.2010.Occurrence and diversity of Xanthomonas campestris pv.campestris in vegetable Brassica fields in Nepal.Plant Dis,94:298-305.
Lema M,Cartea M E,Sotelo T,Velasco P,Soengas P.2012.Discrimination of Xanthomonas campestris pv.campestris races among strains from northwestern Spain by Brassica spp.genotypes and repPCR.European Journal of Plant Pathology,133(1):159-169.
Lv H H,Wang Q B,Yang L M,Fang Z Y,Liu Y M,Zhuang M,Zhang Y Y,Yang Y H,Xie B Y,Wang X W.2014a.Breeding of cabbage(Brassica oleracea L.var.capitata)with Fusarium wilt resistance based on microspore culture and marker-assisted selection.Euphytica,200(3):465-473.
Lv H H,Fang Z Y,Yang L M,Zhang Y Y,Wang Q B,Liu Y M,Zhuang M,Yang Y H,Xie B Y,Liu B,Kang J G,Wang XW.2014b.Mapping and analysis of a novel candidate Fusarium wilt resistance gene FOC1 in brassica oleracea.BMC Genomics,15(1):1094-1103.
Sherf A F,Macnab A A.1986.Vegetable disease and their control.2nd.Canada:John Wiley&Sons Inc:264-266.
Smith E F.1899.The fungous infection of agricultural soils in the United States.Sci Amer Sup,48:19981-19982.
Vicente J G,Taylor J D,Sharpe A G,Parkin I A,Lydiate D J,King G J.2002.Inheritance of race-specific resistance to Xanthomonas campestris pv.campestris in Brassica genomes.Phytopathology,92(10):1134-1141.
Vicente J G,Holub E B.2013.Xanthomonas campestris pv.campestris(cause of black rot of crucifers)in the genomic era is still a worldwide threat to brassica crops.Molecular Plant Pathology,14(1):2-18.
Walker J C.1930.Inheritance of Fusarium resistance in Cabbage.Journal of Agricultural Research,40(1):721-745.
Williams P H,Staub T,Sutton J C.1972.Inheritance of resistance in cabbage to black rot.Phytopathology,62:247-252.
Williams P H.1980.Black rot:a continuing threat to world crucifers.Plant Disease,64(8):736-742.
方智远.2008.甘蓝栽培技术.北京:金盾出版社:1-7.
方智远.2017.中国蔬菜育种学.北京:中国农业出版社:542-547.
耿丽华,迟胜起,焦晓辉,康俊根,田仁鹏.2009.北京延庆县甘蓝枯萎病病原菌的分离及其生物学特性的研究.中国蔬菜,(2):34-37.
龚静,朱玉英,吴晓光.2001.甘蓝黑腐病抗性材料筛选及接种方法的研究.上海农业科技,(4):87.
黄德芬,李成琼,司军,任雪松,宋洪元.2011.甘蓝黑腐病生理小种划分及其抗病性鉴定研究进展.中国蔬菜,(18):6-10.
简元才,钉贯靖久.1994.甘蓝黑腐病抗病性材料的鉴定及筛选.农业新技术,(3):29-30.
康俊根,田仁鹏,耿丽华,陈延阳,简元才,丁云花.2010.甘蓝抗枯萎病种质资源的筛选及抗性基因分布频率分析.中国蔬菜,(2):15-20.
李明远.1997.注意防治十字花科黑腐病.蔬菜,(4):23-24.
李明远,张涛涛,李兴红,严红.2003.十字花科蔬菜枯萎病及其病原鉴定.植物保护,29(6):44-45.
李明远,马永军,姚金亮.2006.警惕十字花科蔬菜枯萎病在我国蔓延.中国植保导刊,26(1):21-22.
李树德.1995.中国主要蔬菜抗病育种进展.北京:科学出版社:617-619.
李锡香.2008.萝卜种质资源描述规范和数据标准.北京:中国农业出版社:82-83.
李永镐,徐丽波.1990.甘蓝黑腐病苗期抗病性鉴定方法的研究.东北农业大学学报,21(2):125-129.
吕红豪,方智远,杨丽梅,谢丙炎,刘玉梅,庄木,张扬勇,杨宇红.2011.甘蓝枯萎病抗源材料筛选及抗性遗传研究.园艺学报,38(5):875-885.
刘佳,冯兰香,蔡少华,方智远,孙培田.1988.结球甘蓝对Tu MV和黑腐病的抗性鉴定.植物保护,14(6):9-11.
彭锐,雷建军.1998.甘蓝抗黑腐病研究现状.西南园艺,(3):29-32.
田仁鹏,康俊根,耿丽华,颉建明,简元才,丁云花.2009.甘蓝枯萎病抗性鉴定方法研究.中国农学通报,25(4):39-42.
王晓武.1992.甘蓝黑腐病抗性遗传分析[硕士论文].北京:中国农业科学院.
杨丽梅,方智远,庄木,张扬勇,吕红豪,刘玉梅,李占省.2016.“十二五”我国甘蓝遗传育种研究进展.中国蔬菜,(11):1-6.
杨宇红,吕红豪,杨翠荣,龚慧芝,杨丽梅,谢丙炎.2011.甘蓝枯萎病苗期抗性鉴定技术及抗源筛选.植物保护学报,38(5):425-431.
张恩慧,程永安,许忠民,王妍妮,马青山.2001.甘蓝3种病害抗源筛选及抗病品种选育研究.西北农林科技大学学报:自然科学版,29(6):30-33.
张黎黎,刘玉梅,田自华,方智远,杨丽梅,庄木,张扬勇,李占省,舒金帅.2012.十字花科蔬菜抗黑腐病育种研究进展.园艺学报,39(9):1727-1738.
张扬,郑建秋,吴学宏,师迎春,谷培云,李健强.2007.北京延庆甘蓝枯萎病发生和危害调查.中国农学通报,23(5):315-320.
张扬,李金萍,周慧敏,李宝聚.2011.十字花科蔬菜细菌性黑腐病的发生规律及防治.中国蔬菜,(17):23-25.
张云霞.2014.甘蓝苗期黑腐病抗性鉴定及抗病相关机理的研究[硕士论文].南京:南京农业大学.
Alvarez A M.2000.Black rot of crucifers.The Netherlands:Kluwer Aczdemic:21-52.
Bain D C.1952.Reaction of brassica seedlings to black rot.Phytopathology,42:497-500.
Bain D C.1955.Resistance of cabbage to black rot.Phytopathology,45:35-37.
Dickson M D,Hunter J E.1987.Inheritance of resistance in cabbage seedlings to black rot.Hortscience,22(1):108-109.
Guo H,Dickson M H,Hunter J E.1991.Brassica napus sources of resistance to black rot in crucifers and inheritance of resistance.Hortscience,26(12):1545-1547.
Hunter J E,Dickson M H,Ludwig J W.1987.Source of resistance to black rot of cabbage expressed in seedlings and adult plants.Plant Disease,71(3):263-266.
Jensen B D,Vicente J G,Manandhar H K,Roberts S J.2010.Occurrence and diversity of Xanthomonas campestris pv.campestris in vegetable Brassica fields in Nepal.Plant Dis,94:298-305.
Lema M,Cartea M E,Sotelo T,Velasco P,Soengas P.2012.Discrimination of Xanthomonas campestris pv.campestris races among strains from northwestern Spain by Brassica spp.genotypes and repPCR.European Journal of Plant Pathology,133(1):159-169.
Lv H H,Wang Q B,Yang L M,Fang Z Y,Liu Y M,Zhuang M,Zhang Y Y,Yang Y H,Xie B Y,Wang X W.2014a.Breeding of cabbage(Brassica oleracea L.var.capitata)with Fusarium wilt resistance based on microspore culture and marker-assisted selection.Euphytica,200(3):465-473.
Lv H H,Fang Z Y,Yang L M,Zhang Y Y,Wang Q B,Liu Y M,Zhuang M,Yang Y H,Xie B Y,Liu B,Kang J G,Wang XW.2014b.Mapping and analysis of a novel candidate Fusarium wilt resistance gene FOC1 in brassica oleracea.BMC Genomics,15(1):1094-1103.
Sherf A F,Macnab A A.1986.Vegetable disease and their control.2nd.Canada:John Wiley&Sons Inc:264-266.
Smith E F.1899.The fungous infection of agricultural soils in the United States.Sci Amer Sup,48:19981-19982.
Vicente J G,Taylor J D,Sharpe A G,Parkin I A,Lydiate D J,King G J.2002.Inheritance of race-specific resistance to Xanthomonas campestris pv.campestris in Brassica genomes.Phytopathology,92(10):1134-1141.
Vicente J G,Holub E B.2013.Xanthomonas campestris pv.campestris(cause of black rot of crucifers)in the genomic era is still a worldwide threat to brassica crops.Molecular Plant Pathology,14(1):2-18.
Walker J C.1930.Inheritance of Fusarium resistance in Cabbage.Journal of Agricultural Research,40(1):721-745.
Williams P H,Staub T,Sutton J C.1972.Inheritance of resistance in cabbage to black rot.Phytopathology,62:247-252.
Williams P H.1980.Black rot:a continuing threat to world crucifers.Plant Disease,64(8):736-742.