山苦瓜资源对枯萎病的抗性评价及理化分析
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摘要
以16个山苦瓜资源为试材,采用抗性接种法,研究了山苦瓜资源的抗病性,并对生理生化指标进行测定,以期为选育优异的苦瓜抗病品系提供参考依据。结果表明:筛选出高抗枯萎病的山苦瓜资源2份(W002、W004),抗病资源1份(Y002),中抗资源2份(S001、S007);理化指标测定结果表明,过氧化物酶(POD)、超氧化物歧化酶(SOD)、苯丙氨酸甲氨酶(PAL)活性与丙二醛(MDA)含量可以作为山苦瓜早期筛选抗性的生理指标。
The 16 germplasm resources of Momordica charantia were used as materials to explore the resistance and physiological and biochemical characteristic by resistance inoculation and characteristic measurment,in order to supply references for screening excellent resistant lines.The results showed that 2 high resistant resourses(W002,W004),1 resistant(Y002)and 2 moderately resistant resources(S001,S007)were screened from the 16 germplasm resources.The physiological and biochemical characteristic of W002,Y002 and S004 were measured,the result showed the activities of POD,SOD,PAL and the content of MDA could be used as resistance index in early screening.
The 16 germplasm resources of Momordica charantia were used as materials to explore the resistance and physiological and biochemical characteristic by resistance inoculation and characteristic measurment,in order to supply references for screening excellent resistant lines.The results showed that 2 high resistant resourses(W002,W004),1 resistant(Y002)and 2 moderately resistant resources(S001,S007)were screened from the 16 germplasm resources.The physiological and biochemical characteristic of W002,Y002 and S004 were measured,the result showed the activities of POD,SOD,PAL and the content of MDA could be used as resistance index in early screening.
引文
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[3] 罗方芳,何自福,余小漫,等.广东苦瓜主要品种对枯萎病的抗性评价[J].广东农业科学,2010(9):72-73.
[4] 陈振东,黄如葵,黎起秦,等.苦瓜种质资源苗期枯萎病抗性鉴定[J].南方农业学报,2014,45(10):1776-1780.
[5] 曾华兰,何炼,刘朝辉,等.苦瓜品种资源抗枯萎病性评价[J].西南农业学报,2011,24(1):137-139.
[6] 赵秀娟,唐鑫,胡开林.苦瓜枯萎病抗性鉴定与抗性遗传规律研究[J].园艺学报,2013,40(4):685-692.
[7] 王学奎.植物生理生化实验原理和技术[M].北京:高等教育出版社,2006.
[8] 汤章域.现代植物生理学实验指南[M].北京:科学出版社,1999.
[9] 于威,郝天龙.几种防御性酶在植物抗病方面的研究进展[J].北京农业,2014(12):133-134.
[10] 张云霞,曾爱松,宋立晓,等.与甘蓝黑腐病相关的酚类物质测定[J]杨州大学学报(农业与生命科学版),2015,36(1):92-96.
[11] 韩庆典,胡晓君,黄坤艳,等.小麦白粉病菌对小麦幼苗MDA 含量及防御酶活性的影响[J]分子植物育种,2016,14(10):2803-2811.
[12] 马艳玲,吴凤芝.枯萎病菌对不同抗性黄瓜品种苯丙氨酸解氨酶的影响[J].沈阳农业大学学报,2006,37(3):335-338.