超声波辅助酶法提取毛木耳多糖工艺条件优化
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摘要
以山东省鱼台县毛木耳为原料,采用超声波辅助酶法进行毛木耳多糖的提取工艺优化。首先采用单因素试验的方法研究液料比、超声波时间、超声波功率、复合酶(复合蛋白酶与纤维素酶质量比为1∶1)、酶解温度4个单因素对毛木耳多糖提取效果的影响。在单因素试验结果的的基础上,进行响应面法试验。依据响应面试验结果分析确定最优超声波辅助酶提取多糖工艺条件为:液料比40∶1(mL/g)、超声波时间31 min、超声波功率160 W、复合酶酶解温度为64℃,在此条件下多糖提取率为14.41%。
Taking the Auricularia polytricha of Yutai county,Shandong province as raw material,and optimization of extraction process of polysaccharides from Auricularia polytricha by ultrasonic assisted enzymatic method. Firstly,single factor experiment was used to study the effects of liquid-to-material ratio,ultra-sonic time,ultrasonic power,and complex enzymolysis temperature(the mass ratio of complex protease to cellulase was 1 ∶ 1)on the extraction of polysaccharides from Auricularia polytricha. Based on the results of singlefactor experiments,response surface method experiments were performed. According to the regression analysis,the optimum conditions for ultrasonic-assisted enzyme extraction of polysaccharides were determined as follows:liquid-to-material ratio 40 ∶ 1(mL/g),ultrasonic time 31 minute,ultrasonic power 160 W,and complex enzyme enzymolysis temperature 64 ℃. Under these conditions,the extraction rate of polysaccharide was 14.41 %.
Taking the Auricularia polytricha of Yutai county,Shandong province as raw material,and optimization of extraction process of polysaccharides from Auricularia polytricha by ultrasonic assisted enzymatic method. Firstly,single factor experiment was used to study the effects of liquid-to-material ratio,ultra-sonic time,ultrasonic power,and complex enzymolysis temperature(the mass ratio of complex protease to cellulase was 1 ∶ 1)on the extraction of polysaccharides from Auricularia polytricha. Based on the results of singlefactor experiments,response surface method experiments were performed. According to the regression analysis,the optimum conditions for ultrasonic-assisted enzyme extraction of polysaccharides were determined as follows:liquid-to-material ratio 40 ∶ 1(mL/g),ultrasonic time 31 minute,ultrasonic power 160 W,and complex enzyme enzymolysis temperature 64 ℃. Under these conditions,the extraction rate of polysaccharide was 14.41 %.
引文
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[10]潘利华,徐学玲,罗建平.水溶性大豆多糖的超声辅助酶法提取条件及抗氧化活性[J].食品科学,2012,33(4):77-81
[11]钱和,张添,刘长虹.改进苯酚-硫酸法测定芦荟多糖含量[J].江苏食品与发酵,2002(3):3-6
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[13]刘军海,黄宝旭,蒋德超.响应面分析法优化艾叶多糖提取工艺研究[J].食品科学,2009,30(2):114-118
[14]刘洋,赵谋明,杨宁,等.响应面分析法优化仙人掌多糖提取工艺的研究[J].食品与机械,2006,22(6):42-44
[15]王丰俊,王建中,郝俊,等.响应面法优化超声波辅助提取桑叶多糖的工艺研究[J].北京林业大学学报,2007,29(5):142-146
[16]陈少青,蒋旭钢,汪财生,等.紫山药多糖超声波辅助提取工艺优化及抗氧化性能研究[J].江苏农业科学,2009(5):231-234
[17]林茹,张福娣,黄碧芳,等.超声波辅助热水提取甘薯多糖工艺[J].亚热带农业研究,2005,1(4):66-68
[18]杨芳,曹银,廖绪标,等.板栗多糖的超声波辅助提取技术[J].湖北农业科学,2012,51(12):2552-2555
[19]杨国伟,危晴,刘卉,等.超声波辅助提取锁阳多糖的工艺研究[J].食品与机械,2013(3):141-144
[2]罗信昌.木耳和毛木耳的极性研究[J].真菌学报,1988,7(1):58-63
[3]余梦瑶,张剑,朱小牧,等.毛木耳多糖对小鼠免疫功能的影响[J].时珍国医国药,2012,23(11):2711-2713
[4]Song G,Du Q.Structure characterization and antitumor activity of anαβ-glucan polysaccharide from Auricularia polytricha[J].Food Research International,2012,45(1):381-387
[5]Yu M,Ma B,Luo X,et al.Molecular diversity of Auricularia polytricha revealed by inter-simple sequence repeat and sequence-related amplified polymorphism markers[J].Current Microbiology,2008,56(3):240-245
[6]Byung-Keun Y,Ji-Young H A,Sang-Chul J,et al.Hypolipidemic effect of an exo-biopolymer produced from submerged mycelial culture of Auricularia polytricha in rats[J].Biotechnology Letters,2002,24(16):1319-1325
[7]吴春敏,陈琼华.毛木耳多糖耐缺氧作用及对心脏和血压的影响[J].中国生化药物杂志,1992(1):21-23
[8]王伟,陈凡,王玉玲.毛木耳多糖提取工艺的研究[J].闽南师范大学学报(自然版),2009,22(3):121-124
[9]陈诚,黄文丽,李小林,等.毛木耳多糖提取研究进展[J].食品与发酵科技,2014,50(4):89-92
[10]潘利华,徐学玲,罗建平.水溶性大豆多糖的超声辅助酶法提取条件及抗氧化活性[J].食品科学,2012,33(4):77-81
[11]钱和,张添,刘长虹.改进苯酚-硫酸法测定芦荟多糖含量[J].江苏食品与发酵,2002(3):3-6
[12]黄海云,张卫明.白芨粗多糖提取方法的比较研究[J].中国野生植物资源,2001(1):14-16
[13]刘军海,黄宝旭,蒋德超.响应面分析法优化艾叶多糖提取工艺研究[J].食品科学,2009,30(2):114-118
[14]刘洋,赵谋明,杨宁,等.响应面分析法优化仙人掌多糖提取工艺的研究[J].食品与机械,2006,22(6):42-44
[15]王丰俊,王建中,郝俊,等.响应面法优化超声波辅助提取桑叶多糖的工艺研究[J].北京林业大学学报,2007,29(5):142-146
[16]陈少青,蒋旭钢,汪财生,等.紫山药多糖超声波辅助提取工艺优化及抗氧化性能研究[J].江苏农业科学,2009(5):231-234
[17]林茹,张福娣,黄碧芳,等.超声波辅助热水提取甘薯多糖工艺[J].亚热带农业研究,2005,1(4):66-68
[18]杨芳,曹银,廖绪标,等.板栗多糖的超声波辅助提取技术[J].湖北农业科学,2012,51(12):2552-2555
[19]杨国伟,危晴,刘卉,等.超声波辅助提取锁阳多糖的工艺研究[J].食品与机械,2013(3):141-144