生姜外用对大、小鼠痔疮模型的影响
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摘要
目的:探讨生姜外用对大、小鼠痔疮模型的疗效。方法:SPF级SD大鼠60只,分为空白组,模型组,马应龙麝香痔疮膏组(7.5 g·kg~(-1)),生姜糊大、小剂量组(10,5 g·kg~(-1)),采用生姜糊外敷涌泉穴方法,观察对由75%乙酸溶液0.05 mL注射于大鼠肛门外周皮下所致大鼠痔疮模型的影响,酶联免疫吸附测定(ELISA)检测血清白细胞介素-1β(IL-1β),白细胞介素-6(IL-6),肿瘤坏死因子-α(TNF-α),一氧化氮(NO)水平,苏木素-伊红(HE)染色评价直肠组织病理形态的改变; SPF级KM小鼠60只,分为空白组,模型组,马应龙麝香痔疮膏组(7.5 g·kg~(-1)),生姜精油大、小剂量组(0.06,0.03 mL,每天3次),采用生姜精油外涂肛周方法,观察对由20%乙酸溶液0.05 mL内推入小鼠肛门(保持1 min)所致小鼠痔疮模型的影响,观察肛周局部肿胀溃疡程度并进行评分,ELISA检测血清IL-1β,TNF-α水平,HE染色观察直肠局部组织形态的影响。结果:生姜精油外敷大鼠涌泉穴治疗痔疮实验,与正常组比较,模型组血清IL-1β,TNF-α,IL-6,NO水平显著升高(P<0.01),直肠组织病理损伤严重;与模型组比较,各给药组可降低血清IL-6,NO,IL-1β,TNF-α水平(P<0.05,P<0.01),减轻直肠组织损伤程度(P<0.01);生姜精油外涂肛周治疗痔疮实验,与正常组比较,模型组肛周肿胀、溃疡程度损伤严重(P<0.01),血清IL-1β,TNF-α水平显著升高(P<0.01),直肠组织病理损伤严重;与模型组比较,各给药组可改善肛周的病理状况(P<0.01),降低血清IL-1β,TNF-α水平(P<0.01),减轻直肠组织损伤程度(P<0.01)。结论:生姜外用可有效抑制肛周肿胀和溃疡程度,对大、小鼠痔疮模型有很好的治疗作用。
Objective:To explore the effect of Zingiberis Rhizoma Recens(ZRR) on hemorrhoids in mice and rats.Method:Sixty SPF-grade SD rats were divided into blank group,model group,Ma Yinglong Shexiang hemorrhoid ointment group(7.5 g·kg~(-1)),and large and small-dose ZRR paste groups(10,5 g·kg~(-1)).ZRR paste was applied in Yongquan acupoint to observe the effect of 0.05 mL injection with 75% acetic acid solution on hemorrhoids induced by subcutaneous anus in rats,the levels of interleukin-1β(IL-1β),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),nitric oxide(NO) in the serum were detected by enzyme linked immunosorbent assay(ELISA),and the rectal histopathology was detected by hematoxylin-eosin(HE).Sixty SPF-grade KM mice were divided into blank group,model group,MA Ying-long Shexiang hemorrhoid ointment group(7.5 g·kg~(-1)),and large and small-dose ginger essential oil groups(0.06,0.03 mL,three times a day).ZRR paste was applied in crissum to observe the effect of injection of 20% acetic acid solution 0.05 mL(maintaining for 1 min) on hemorrhoids in mice induced by anus.The degree of local swelling ulcer around the anus and score was observed,levels of IL-1β,TNF-α in the serum were determined by ELISA,and the rectal histopathology was detected by HE staining.Result:In the experimental study on treating hemorrhoids with ZRR paste applied on Yongquan point of rats,compared with normal group,serum levels of IL-1β,IL-6,TNF-α,NO in model group were significantly higher(P<0.01).The pathological injury of rectum was serious.Compared with model group,serum levels of IL-1β,IL-6,TNF-α,NO were decreased in each administration group(P<0.05,P<0.01),and the degree of rectal tissue injury was relieved(P<0.01).In the experiment of external application of ZRR essential oil around the anus for treating hemorrhoids,compared with normal group,perianal swelling and severe ulcer injury were observed in the model group(P<0.01).The serum levels of IL-1β,TNF-α in model group were significantly higher(P<0.01),and the pathological injury of rectum was serious.Compared with model group,each administration group showed the alleviation in perianal pathological conditions(P<0.01),the reduction in serum IL-1β,TNF-α levels(P<0.01),and the relief in the extent of rectal tissue injury(P<0.01).Conclusion:External application of ZRR can effectively inhibit perianal swelling and ulcer degree,with a good therapeutic effect on hemorrhoids model in rats and mice.
Objective:To explore the effect of Zingiberis Rhizoma Recens(ZRR) on hemorrhoids in mice and rats.Method:Sixty SPF-grade SD rats were divided into blank group,model group,Ma Yinglong Shexiang hemorrhoid ointment group(7.5 g·kg~(-1)),and large and small-dose ZRR paste groups(10,5 g·kg~(-1)).ZRR paste was applied in Yongquan acupoint to observe the effect of 0.05 mL injection with 75% acetic acid solution on hemorrhoids induced by subcutaneous anus in rats,the levels of interleukin-1β(IL-1β),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),nitric oxide(NO) in the serum were detected by enzyme linked immunosorbent assay(ELISA),and the rectal histopathology was detected by hematoxylin-eosin(HE).Sixty SPF-grade KM mice were divided into blank group,model group,MA Ying-long Shexiang hemorrhoid ointment group(7.5 g·kg~(-1)),and large and small-dose ginger essential oil groups(0.06,0.03 mL,three times a day).ZRR paste was applied in crissum to observe the effect of injection of 20% acetic acid solution 0.05 mL(maintaining for 1 min) on hemorrhoids in mice induced by anus.The degree of local swelling ulcer around the anus and score was observed,levels of IL-1β,TNF-α in the serum were determined by ELISA,and the rectal histopathology was detected by HE staining.Result:In the experimental study on treating hemorrhoids with ZRR paste applied on Yongquan point of rats,compared with normal group,serum levels of IL-1β,IL-6,TNF-α,NO in model group were significantly higher(P<0.01).The pathological injury of rectum was serious.Compared with model group,serum levels of IL-1β,IL-6,TNF-α,NO were decreased in each administration group(P<0.05,P<0.01),and the degree of rectal tissue injury was relieved(P<0.01).In the experiment of external application of ZRR essential oil around the anus for treating hemorrhoids,compared with normal group,perianal swelling and severe ulcer injury were observed in the model group(P<0.01).The serum levels of IL-1β,TNF-α in model group were significantly higher(P<0.01),and the pathological injury of rectum was serious.Compared with model group,each administration group showed the alleviation in perianal pathological conditions(P<0.01),the reduction in serum IL-1β,TNF-α levels(P<0.01),and the relief in the extent of rectal tissue injury(P<0.01).Conclusion:External application of ZRR can effectively inhibit perianal swelling and ulcer degree,with a good therapeutic effect on hemorrhoids model in rats and mice.
引文
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[21]王姝,梁翠茵.生姜药理作用的研究进展[J].卫生职业教育,2014,32(22):148-150.
[22]潘海邦,于博,吴国泰,等.三黄片调和物外用安全性研究[J].中国实验方剂学杂志,2011,17(15):238-240.
[23]刘文彬,梁庆,王晖,等.外用丹参酮乳膏抗痤疮药效及机制[J].中国实验方剂学杂志,2012,18(14):212-216.
[24]刘史佳,申龙树,戴国梁,等.IL-17、IL-6、TNF-α细胞因子在痔疮患者中的表达[J].药学与临床研究,2016,24(3):201-204.
[25]Ouyang Q,Tandon R,Goh K L,et al.The emergence of inflammatory bowel disease in the Asian Pacific re-gion[J].Curr Opin Gastroenterol,2005,21(4):408-413.
[26]Vermeire S.Review article:genetic susceptibility and application of genetic testing in clinical management of inflammatory bowel disease[J].Aliment Pharmacol,2010,24(3):2-10.
[27]田硕,苗明三.基于神经-内分泌-免疫网络探讨中药外用作用机制-“三微调平衡”[J].中国实验方剂学杂志,2019,doi.org/10.13422/j.cnki.syfjx.20190436.
[28]巩仔鹏,李梅,熊荻菲菲,等.基于体内外炎症模型研究羊耳菊提取物的抗炎活性[J].天然产物研究与开发,2017,29(12):2050-2055.
[29]胡艳丽.浅谈涌泉穴临床应用[J].针灸临床杂志,2012,28(8):39-41.
[2]杨凤.痔疮的中医药治疗概述[J].中医临床研究,2013,5(19):119-121.
[3]刘加乐.痔疮的临床治疗进展[J].临床合理用药杂志,2017,10(32):173-174.
[4]田硕,白明,武晏屹,等.中药临床外治技术规范的现状及发展趋势[J].中国实验方剂学杂志,2018,doi.org/10.13422/j.cnki.syfjx.20182201.
[5]李胜.痔疮的发病与治疗综述[J].中国医药指南,2014,12(1):43-44.
[6]徐惠.中药外敷涌泉穴治疗口腔溃疡的疗效观察[J].中国民间疗法,2011,19(11):79.
[7]苗明三,张梦飞,田硕.中药痔疮动物模型制备规范(草案)起草说明[J].中药药理与临床,2017,33(5):212-215.
[8]李雪梅,邵燕,周建平.通便化痔颗粒对痔疮模型大鼠的疗效[J].中国比较医学杂志,2012,22(5):22-24,80.
[9]于舒雁,魏荣锐,苗明三.不同品种威灵仙水煎液外用对大鼠痔疮、佐剂性关节炎模型的影响[J].中国现代应用药学,2014,31(4):391-397.
[10]白明,刘丹丹,缪君娴,等.不同品种大黄油糊外用抗炎作用及对大鼠皮肤溃疡、痔疮模型的影响[J].中国现代应用药学,2013,30(6):575-581.
[11]黄华利,李娟,何海霞,等.五味痔疮胶囊的镇痛、止血、通便和抗菌作用研究[J].中国药房,2014,25(43):4056-4059.
[12]黄一挚,黄明政,陈朝,等.壮药痔疮熏洗液的止血、镇痛、抗炎和抗菌作用研究[J].中国药房,2016,27(34):4817-4820.
[13]贾立刚,宋立峰,孙清晨.中医药治疗痔疮的研究概述[J].环球中医药,2012,5(4):317-320.
[14]张庆莲,黄娟,皮凤娟,等.中医药非手术治疗痔疮的研究进展[J].中国医院用药评价与分析,2014,14(2):188-190.
[15]羊少艺,贺平.中医内外治疗痔疮研究进展[J].亚太传统医药,2016,12(16):92-94.
[16]苗明三,白明.中药外用药理实验技术规范(草案)[J].中药药理与临床,2014,30(4):128-131.
[17]郭琳,王灿,刘佃温,等.中药外用治疗痔疮特点分析[J].中医学报,2015,30(2):202-205.
[18]XU Y K,ZHU F G,FENG E Y,et al.External therapies of traditional Chinese medicine combined with sodium hyaluronate injected in articular cavity therapy on knee osteoarthritis:meta-analysis[J].China J Chin Mater Med,2018,43(9):1934-1939.
[19]曹利华,白明,苗明三,等.中药外治的“双微调平衡”机制探析[J].中华中医药杂志,2018,33(3):819-823.
[20]李娟,王智民,高慧敏.炮制对生姜及其不同炮制品中挥发性成分的影响[J].中国实验方剂学杂志,2012,18(19):77-81.
[21]王姝,梁翠茵.生姜药理作用的研究进展[J].卫生职业教育,2014,32(22):148-150.
[22]潘海邦,于博,吴国泰,等.三黄片调和物外用安全性研究[J].中国实验方剂学杂志,2011,17(15):238-240.
[23]刘文彬,梁庆,王晖,等.外用丹参酮乳膏抗痤疮药效及机制[J].中国实验方剂学杂志,2012,18(14):212-216.
[24]刘史佳,申龙树,戴国梁,等.IL-17、IL-6、TNF-α细胞因子在痔疮患者中的表达[J].药学与临床研究,2016,24(3):201-204.
[25]Ouyang Q,Tandon R,Goh K L,et al.The emergence of inflammatory bowel disease in the Asian Pacific re-gion[J].Curr Opin Gastroenterol,2005,21(4):408-413.
[26]Vermeire S.Review article:genetic susceptibility and application of genetic testing in clinical management of inflammatory bowel disease[J].Aliment Pharmacol,2010,24(3):2-10.
[27]田硕,苗明三.基于神经-内分泌-免疫网络探讨中药外用作用机制-“三微调平衡”[J].中国实验方剂学杂志,2019,doi.org/10.13422/j.cnki.syfjx.20190436.
[28]巩仔鹏,李梅,熊荻菲菲,等.基于体内外炎症模型研究羊耳菊提取物的抗炎活性[J].天然产物研究与开发,2017,29(12):2050-2055.
[29]胡艳丽.浅谈涌泉穴临床应用[J].针灸临床杂志,2012,28(8):39-41.