建立一种新型痔疮动物模型的方法研究
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摘要
目的探索和建立一种较为接近人体痔疮实际病理症状的新型实用痔疮动物模型。方法根据人类痔疮的肛管局部静脉曲张、血流瘀滞、微循环障碍、局部炎症等病理特征及药理学原理,选用几种药物试剂包括微循环阻滞剂、肠道平滑肌运动抑制剂及致炎、致肿胀试剂等,以合适剂量联合应用,采用肛直肠局部皮下注射给药,诱发SD大鼠痔疮症状的发生。结果通过为期2周的造模实验及观测发现,此种造模方法能够产生类似于人类痔疮的临床症状表现,如肛周红肿、排便不畅等,并显著增加模型动物的肛周直径。在大鼠肛直肠病理切片的观察中发现,模型动物肛直肠部有较明显的血管增生、血栓、痔核形成及炎症、肉芽肿等病理变化,能较好地反映痔疮病理症状。结论此方法可以建立起接近临床疾病真实表现且稳定可靠的痔疮动物模型。
OBJECTIVE To explore and establish a new and practical hemorrhoids model which is relatively closer to the actual pathological symptoms of human hemorrhoids. METHODS According to the pathological characteristics of human hemorrhoids, such as local varicose veins of the anal canal, blood stasis, microcirculation disorder and local inflammation,combined with the principles of pharmacology, chose several drugs and reagents including microcirculation blockers, intestinal smooth muscle movement inhibitors, pro-inflammation and swelling reagents, at appropriate doses administrated together via local subcutaneous injection in anal canal, to induce hemorrhoids symptoms in SD rats. RESULTS After the 2-week modeling experiment period and the subsequent observations, this model-building method produced a series of symptoms similar to the clinical symptoms of human hemorrhoids, such as inflamed crissum and constipation, and significantly increased the crissum diameter of the modeling animals. In the histological and pathological observations of the anal canal of rats, the pathological changes including vessels hyperplasia, thrombus, haemorrhoids, inflammation and granulomatous were observed in the animal model, which could well simulate and reflect the pathological symptoms of human hemorrhoids. CONCLUSION This method can establish a stable and reliable animal model of hemorrhoids with manifestations of the clinical disease symptoms.
OBJECTIVE To explore and establish a new and practical hemorrhoids model which is relatively closer to the actual pathological symptoms of human hemorrhoids. METHODS According to the pathological characteristics of human hemorrhoids, such as local varicose veins of the anal canal, blood stasis, microcirculation disorder and local inflammation,combined with the principles of pharmacology, chose several drugs and reagents including microcirculation blockers, intestinal smooth muscle movement inhibitors, pro-inflammation and swelling reagents, at appropriate doses administrated together via local subcutaneous injection in anal canal, to induce hemorrhoids symptoms in SD rats. RESULTS After the 2-week modeling experiment period and the subsequent observations, this model-building method produced a series of symptoms similar to the clinical symptoms of human hemorrhoids, such as inflamed crissum and constipation, and significantly increased the crissum diameter of the modeling animals. In the histological and pathological observations of the anal canal of rats, the pathological changes including vessels hyperplasia, thrombus, haemorrhoids, inflammation and granulomatous were observed in the animal model, which could well simulate and reflect the pathological symptoms of human hemorrhoids. CONCLUSION This method can establish a stable and reliable animal model of hemorrhoids with manifestations of the clinical disease symptoms.
引文
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[13]AKKOL E K,ERCIL D.Antinociceptive and anti-inflammatory activities of some linaria species from turkey[J].Pharm Biol,2009,47(3):188-194.
[14]AKKOL E K,GUVENC A,YESILADA E.A comparative study on the antinociceptive and anti-inflammatory activities of five Juniperus taxa[J].J Ethnopharmacol,2009,125(2):330-336.
[15]ZHUANG Q,ZHANG C Y,CHENG Y,et al.Experimental study on the diagnosis of slow transit constipation rats with colon transport particles[J].Chin J Mod Appl Pharm(中国现代应用药学),2017,34(12):1675-1678.
[16]CAI Z B,ZHAO X,ZHAO P J,et al.Establishment of rat model of hyperuricemia combined with nonalcoholic fatty liver disease[J].Chin J Mod Appl Pharm(中国现代应用药学),2017,34(9):1234-1238.
[2]MENG W.The formation,treatment and prevention of hemorrhoids[J].Chin J Mod Drug Appl(中国现代药物应用),2011,5(7):126-127.
[3]HASKINS I N,HOLZMACHER J,OBIAS V,et al.The use of transanal hemorrhoidal dearterialization for treatment of hemorrhoid disease at a single institution[J].Am Surg,2016,82(12):1160-1162.
[4]BAI M,LIU D D,LIAO J X,et al.Anti-inflammatory effects of oil paste of different varieties rhubarb by external use on skin ulcers model and hemorrhoids model in rats[J].Chin JMod Appl Pharm(中国现代应用药学),2013,30(6):575-581.
[5]DEY Y N,WANJARI M M,KUMAR D,et al.Curative effect of Amorphophallus paeoniifolius tuber on experimental hemorrhoids in rats[J].J Ethnopharmacol,2016,192(21):183-191.
[6]LI X M,SHAO Y,ZHOU J P.Tongbianhuazhi Keli on the hemorrhoids rat animal model effecacy studies[J].Chin J Comp Med(中国比较医学杂志),2012,22(5):22-24.
[7]XU C,ZHOU Q,ZHU X X,et al.Research on Zhilou Fumigating and washing therapy for hemorrhoid models[J].Chin Arch Tradit Chin Med(中华中医药学刊),2015,33(9):2228-2230.
[8]张宏辉,杜位良.痔疮的中西医研究进展[J].亚太传统医药,2016,12(15):103-105.
[9]WANG J,LI D.Therapeutic drugs for hemorrhoids[J].Chin JMod Appl Pharm(中国现代应用药学),2009,26(7):545-549.
[10]AZEEMUDDIN M,VISWANATHA G L,RAFIQ M,et al.An improved experimental model of hemorrhoids in rats:evaluation of antihemorrhoidal activity of an herbal formulation[J].ISRN Pharmacol,2014(2014):530931.Doi:10.1155/2014/530931.
[11]MA Z G,JIANG N,YU M Y,et al.Progress in research on animal models of hemorrhoids[J].Acta Lab Anim Sci Sin(中国实验动物学报),2010,18(5):446-450.
[12]YANG Y,GONG Y.The Local inflammation and histopathological effects of YinZhi anorectal smoked lotion act on similar hemorrhoid model of rats[J].J Yunnan Univ Tradit Chin Med(云南中医学院学报),2014,37(3):11-14.
[13]AKKOL E K,ERCIL D.Antinociceptive and anti-inflammatory activities of some linaria species from turkey[J].Pharm Biol,2009,47(3):188-194.
[14]AKKOL E K,GUVENC A,YESILADA E.A comparative study on the antinociceptive and anti-inflammatory activities of five Juniperus taxa[J].J Ethnopharmacol,2009,125(2):330-336.
[15]ZHUANG Q,ZHANG C Y,CHENG Y,et al.Experimental study on the diagnosis of slow transit constipation rats with colon transport particles[J].Chin J Mod Appl Pharm(中国现代应用药学),2017,34(12):1675-1678.
[16]CAI Z B,ZHAO X,ZHAO P J,et al.Establishment of rat model of hyperuricemia combined with nonalcoholic fatty liver disease[J].Chin J Mod Appl Pharm(中国现代应用药学),2017,34(9):1234-1238.