4种铁线莲属植物种子萌发特性
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摘要
本研究以转子莲(Clematis patens)、棉团铁线莲(C.hexapetala)、大叶铁线莲(C.heracleifolia)和齿叶铁线莲(C.serratifolia)4种种子为材料,研究激素及浸种温度对其萌发的影响。结果表明,转子莲最适浸种温度为35℃,棉团铁线莲、大叶铁线莲和齿叶铁线莲最适浸种温度均为25℃,其发芽率分别为24.87%、93.24%、80.94%和68.21%。400mg·L~(-1)赤霉素(GA3)有利于转子莲种子萌发,其发芽率为19.17%;棉团铁线莲在5mg·L~(-1)细胞分裂素(6-BA)和500mg·L~(-1) GA3中萌发情况最好,其发芽率为90.56%和90.50%;10mg·L~(-1)6-BA能显著促进大叶铁线莲种子萌发,其发芽率为88.26%;5mg·L~(-1) GA3有利于齿叶铁线莲萌发,其发芽率为53.00%。
The effects of hormones and soaking temperature on germination characteristics of the seeds of four species of Clematis(C.patens,C.hexapetala,C.heracleifolia,and C.serratifolia)were examined in this study.For the seeds of C.patens,the best germination results were achieved using a water soaking treatment at 35℃,whereas for the seeds of C.hexapetala,C.heracleifolia,and C.serratifolia a soaking temperature of 25 ℃ produced the best results.The germination rates of the four species of seeds were 24.87%,93.24%,80.94%,and 68.21%,respectively.400 mg·L~(-1) GA3 treatment was optimal for seeds of C.patens,producing agermination rate of 19.17%.For the seeds of C.hexapetala,5 mg·L~(-1) 6-BA and 500 mg·L~(-1) GA3 treatments produced germination rates of 90.56%and 90.50%,respectively.The seeds of C.heracleifolia showed the best results using a 10 mg·L~(-1) 6-BA treatment,with a germination rate of 88.26%.The seeds of C.serratifolia germinated best following treatment with 5 mg·L~(-1) GA3,with a germination rate of 53.00%.
The effects of hormones and soaking temperature on germination characteristics of the seeds of four species of Clematis(C.patens,C.hexapetala,C.heracleifolia,and C.serratifolia)were examined in this study.For the seeds of C.patens,the best germination results were achieved using a water soaking treatment at 35℃,whereas for the seeds of C.hexapetala,C.heracleifolia,and C.serratifolia a soaking temperature of 25 ℃ produced the best results.The germination rates of the four species of seeds were 24.87%,93.24%,80.94%,and 68.21%,respectively.400 mg·L~(-1) GA3 treatment was optimal for seeds of C.patens,producing agermination rate of 19.17%.For the seeds of C.hexapetala,5 mg·L~(-1) 6-BA and 500 mg·L~(-1) GA3 treatments produced germination rates of 90.56%and 90.50%,respectively.The seeds of C.heracleifolia showed the best results using a 10 mg·L~(-1) 6-BA treatment,with a germination rate of 88.26%.The seeds of C.serratifolia germinated best following treatment with 5 mg·L~(-1) GA3,with a germination rate of 53.00%.
引文
[1]王文采,李良千.铁线莲属一新分类系统.植物分类学报,2005,43(5):431-488.WANG W C,LI L Q.A new system of classification of the genus Clematis.Acta Phytotaxonomica Sinica,2005,43(5):431-488.
[2] TAMURA M.Morphology,ecology and phylogeny of the RanunculaceaeⅦ.Science Reports Osaka University,1967,16:21-43.
[3] WANG W T,BRUEE B.Clemtis L.//Wu Z Y,Rven P H(eds).Flora of China(Voi.6).Beijing:Science Press,2001:333-386.
[4] LU S,KONG S J,LIANG L Y.Karyotype analysis on 11species of the genus Clematis.Botanical Society,2014,37(4):601-608.
[5]中国科学院中国植物志编辑委员会.中国植物志.北京:科学出版社,2013.Editorial Committee of Chinese Journal of Plant of Chinese Academy of Sciences.Flora of China.Beijing:Science Press,2013.
[6]马雷昌,李道同,付桂云.适于北方园林应用的铁线莲属植物资源的开发利用.山东林业科技,2003(3):33-34.MA L C,LI D T,FU G Y.The exploitation and utilization of Clematis L.in the garden of the northern city.Shandong Forestry Science and Technology,2003(3):33-34.
[7]潘杰,王佳巍,李滨胜.大叶铁线莲引种栽培与有性繁殖研究.防护林科技,2015(2):31-33.PAN L,WANG J W,LI B S.Introduction cultivation and sexual reproduction of Clematis heracleifolia.Protection Forest Science and Technology,2015(2):31-33.
[8]王金侠.4种铁线莲属植物种子发芽特性及其幼苗抗旱性.哈尔滨:东北林业大学硕士学位论文,2014.WANG J X.Study on seeds germination characteristics and seedlings drought resistance of four kinds of Clematis L.Master Thesis.Harbin:Northeast Forestry University,2014.
[9]田宏,刘洋,张鹤山,蔡化,李晓峰,索效军,张年,陈明新.扁穗雀麦种子萌发条件的研究.草业科学,2009,26(7):88-93.TIAN H,LIU Y,ZHANG H S,CAI H,LI X F,SUO X J,ZHANG N,CHEN M X.Study on the germination conditions of Bromus cartharticus.Pratacultural Science,2009,26(7):88-93.
[10]王荷.野生花卉用于野花草地的营建初探.北京:北京林业大学硕士学位论文,2009.WANG H.Preliminary studies on making wildflower meadow with wildflowers.Master Thesis.Beijing:Beijing Forestry University,2009.
[11]张雷,张春辉,吕俊平,王晨阳,张莹莹,卜海燕,杜国祯.光照强度对青藏高原东缘九种紫草科植物种子萌发的影响.兰州大学学报(自然科学版),2011,47(5):67-72.ZHANG L,ZHANG C L,LYU J P,WANG C Y,ZHANG Y Y,BU H Y,DU G Z.Effect of light intensity on seed germination in nine boraginaceae species on the eastern Qinghai-Tibet plateau.Journal of Lanzhou University(Natural Sciences),2011,47(5):67-72.
[12]黎彩霞,廖明安,魏志强,沈文,任雅君.不同水温浸种对毛蕊铁线莲种子发芽指标的影响.安徽农业科学,2011,39(30):18479-18480.LI C X,LIAO M A,WEI Z Q,SHEN W,REN Y J.Effects of different water temperature on seeds germination of Clematis lasiandra Maxim.Journal of Anhui Agricultural Sciences,2011,39(30):18479-18480.
[13]孙云波,陈翔,陈垣,周传猛,白德涛.种荚预处理对红芪种子发芽特性的影响.草业学报,2015,24(6):159-167.SUN Y B,CHEN X,CHEN Y,ZHOU C M,BAI D T.Effects of pod pre-treatments on the germination traits of Hedysarum polybotrys seeds.Acta Prataculturae Sinica,2015,24(6):159-167.
[14]黄平权,冯世鑫.萝芙木种子发芽的试验研究.广西中医药大学学报,2012,15(1):61-63.HUANG P Q,FENG S X.Study on the germination of seeds of Rauvolfia verticillata.Journal of Guangxi Traditional Chinese Medical University,2012,15(1):61-63.
[15]张树芬.不同催芽处理及芽苗移栽对云南石梓种子发芽及幼苗生长的影响.种子,2015,34(4):123-125.ZHANG S F.Effects of different acelerating germination treatment and transplanting on seed germination and seedling growth of Koombar.Seed,2015,34(4):123-125.
[16]李鲜花,刘永华,罗彩云,刘翠英,贺英.不同处理对白皮松种子发芽率的影响.榆林学院学报,2014,24(4):21-23.LI X H,LIU Y H,LUO C Y,LIU C Y,HE Y.Influence of different dispositions on germination rate of Pinus bungeana.Journal of Yulin University,2014,24(4):21-23.
[17] NIKOLAEVA M G.Physology of Deep Dormancy in Seeds.Washington,DC:National Science Foundation,1967.
[18]许世泉,贾秀梅,王志清,焉石,张瑞,逢世峰.东北铁线莲种胚后熟发育研究.特产研究,2010,32(4):25-26,38.XU S Q,JIA X M,WANG Z Q,YAN S,ZHANG R,FENG S F.Study on seed embryo postmaturation and development of Clematis mandshurica Rupr.Special Wild Economic Animal and Plant Research,2010,32(4):25-26,38.
[19]刘华,蒋齐,李如来,李明,张清云.不同植物激素对甘草种子萌发的影响.宁夏农林科技,2013,54(2):19-21.LIU H,JIANG Q,LI R L,LI M,ZHANG Q Y.A study of effect of various plant hormones on seed germination of Glycyrrhiza uralensis.Ningxia Journal of Agriculture and Forestry Science and Technology,2013,54(2):19-21.
[20] BIALECKA B.,KEPCZYSKI J.Germination,α-,β-amylase and total dehydrogenase activities of Amaranthus caudatus seeds under water stress in the presence of ethephon or gibberellin A3.Acta Biologica Cracoviensia Series Botanica,2010,52(1):7-12.
[21] DEBEAUJON I,KOORNNEEF M.Gibberellin requirement for Arabidopsis seed germination is determined both by testa characteristics and embryonic abscisic acid.Plant Physiology,2000,122(2):415-424.
[22]许世泉,王振兴,邵财,王志清,逄世峰,张瑞.东北铁线莲种胚后熟过程中内源激素动态变化.特产研究,2011,33(2):37-39.XU S Q,WANG Z X,SHAO C,WANG Z Q,PANG S F,ZHANG R.Morphological development of seed embryo in its postripening and dynamic changes of endogenesis hormone in Clematis mandshurica.Special Wild Economic Animal and Plant Research,2011,33(2):37-39.
[23]邹竣竹,韩蕾,李德颖,孙振元.赤霉素和生长素浸种对野牛草种子萌发及幼苗生长生理的影响草业科学,2017,34(9):1838-1846.ZOU J Z,HAN L,LI D Y,SUN Z Y.Seed soaking in exogenous gibberellin and auxin and their impact on seed germination and seedling physiology of Buchloe dactyloides(Nutt.)Engelm.Pratacultural Science,2017,34(9):1838-1846.
[24]孙丹,张金政,孙国峰,李晓东,何卿.细叶鸢尾种子休眠原因及解除休眠的方法研究.草业学报,2011,20(6):109-117.SUN D,ZHANG J Z,SUN G F,LI X D,HE Q.A study on the causes of Iris tenuifolin dormancy and the methods for breaking dormancy.Acta Prataculturae Sinica,2011,20(6):109-117.
[25]浦新春,韩建国,李敏.结缕草种子脱落酸含量及打破休眠的研究.草地学报,1994,2(1):30-35.PU X C,HAN J G,LI M.Quantity of abscisic acid and break dormancy in Zoysiagrass seed.Acta Agrestia Sinca,1994,2(1):30-35.
[26]邹琇莹,DAVID W FOUNTIN,Ed R MORGAN.赤霉素及机械处理的相互作用对破除Sandersonia aurantiaca种子休眠的研究.草业学报,2003,12(5):70-76.ZOU X Y,DAVID W FOUNTIN,Ed R MORGAN.Seed dormancy of Sandersonia aurantiaca broken by interaction of GA3and mechanical treatment.Acta Prataculturae Sinica,2003,12(5):70-76.
[27]韩云华,王彦荣,陶奇波.种子激素引发.草业科学,2016,33(12):2494-2502.HANY H,WANG Y R,TAO Q B.Advances of seed hormonal priming.Pratacultural Science,2016,33(12):2494-2502.
[28]郑芳昊,潘超美,赖珍珍,夏静,梁钻姬,刘欣.野菊花种子发芽特性的研究.中药材,2012,35(3):351-354.ZHENG F H,PAN C M,LAI Z Z,XIA J,LIANG Z J,LIU X.Study on germination characteristic of seed of Dendranthema indicum.Journal of Chinese Medicinal Materials,2012,35(3):351-354.
[29]焦德志,龚孟,潘学岩,朱蕾.不同植物激素对羊草种子萌发和幼苗生长的影响.安徽农业科学,2010,38(3):1188-1190.JIAO D Z,GONG M,PAN X Y,ZHU L.Effects of various phytohormones on germination and growth of L.chinensis seeds.Journal of Anhui Agricultural Sciences,2010,38(3):1188-1190.
[30]王磊,周余华,关雪莲,张虎,陈少卿.GA3和6-BA对粉绿铁线莲种子发芽特性的影响.种子,2010,29(3):44-50.WANG L,ZHOU Y H,GUAN X L,ZHANG H,CHEN S Q.Effects of plant growth regulators GA3and 6-BA on seed germinaion characteristics of Clematis glauca.Seed,2010,29(3):44-50.
[31]刘志高,季梦成,杨彦鹏,沈永宝.3种铁线莲属植物种子萌发特性研究.种子,2015,34(6):30-33.LIU Z G,JI M C,YANG Y P,SHEN Y B.Study on germination characteristics of three species from Clematis.Seed,2015,34(6):30-33.
[32]王娜,王奎玲,刘庆华,刘庆超.三种铁线莲种子萌发研究.2016年中国观赏园艺学术研讨会论文集.长沙:中国园艺学会,2016:492-495.WANG N,WANG K L,LIU Q H,LIU Q C.Study on the germination characteristics of three Clematis.Proceedings of 2016China Ornamental Horticulture Academic Seminar.Changsha:Chinese Society for Horticultural Science,2016:492-495.
[33]王非,王金侠,李强,何淼.GA3和IAA处理对4种铁线莲种子萌发的影响.草业科学,2014,31(4):672-676.WANG F,WANG J X,LI Q,HE M.Effects of GA3and IAA on the germination of four species of Clematis seeds.Pratacultural Science,2014,31(4):672-676.
[2] TAMURA M.Morphology,ecology and phylogeny of the RanunculaceaeⅦ.Science Reports Osaka University,1967,16:21-43.
[3] WANG W T,BRUEE B.Clemtis L.//Wu Z Y,Rven P H(eds).Flora of China(Voi.6).Beijing:Science Press,2001:333-386.
[4] LU S,KONG S J,LIANG L Y.Karyotype analysis on 11species of the genus Clematis.Botanical Society,2014,37(4):601-608.
[5]中国科学院中国植物志编辑委员会.中国植物志.北京:科学出版社,2013.Editorial Committee of Chinese Journal of Plant of Chinese Academy of Sciences.Flora of China.Beijing:Science Press,2013.
[6]马雷昌,李道同,付桂云.适于北方园林应用的铁线莲属植物资源的开发利用.山东林业科技,2003(3):33-34.MA L C,LI D T,FU G Y.The exploitation and utilization of Clematis L.in the garden of the northern city.Shandong Forestry Science and Technology,2003(3):33-34.
[7]潘杰,王佳巍,李滨胜.大叶铁线莲引种栽培与有性繁殖研究.防护林科技,2015(2):31-33.PAN L,WANG J W,LI B S.Introduction cultivation and sexual reproduction of Clematis heracleifolia.Protection Forest Science and Technology,2015(2):31-33.
[8]王金侠.4种铁线莲属植物种子发芽特性及其幼苗抗旱性.哈尔滨:东北林业大学硕士学位论文,2014.WANG J X.Study on seeds germination characteristics and seedlings drought resistance of four kinds of Clematis L.Master Thesis.Harbin:Northeast Forestry University,2014.
[9]田宏,刘洋,张鹤山,蔡化,李晓峰,索效军,张年,陈明新.扁穗雀麦种子萌发条件的研究.草业科学,2009,26(7):88-93.TIAN H,LIU Y,ZHANG H S,CAI H,LI X F,SUO X J,ZHANG N,CHEN M X.Study on the germination conditions of Bromus cartharticus.Pratacultural Science,2009,26(7):88-93.
[10]王荷.野生花卉用于野花草地的营建初探.北京:北京林业大学硕士学位论文,2009.WANG H.Preliminary studies on making wildflower meadow with wildflowers.Master Thesis.Beijing:Beijing Forestry University,2009.
[11]张雷,张春辉,吕俊平,王晨阳,张莹莹,卜海燕,杜国祯.光照强度对青藏高原东缘九种紫草科植物种子萌发的影响.兰州大学学报(自然科学版),2011,47(5):67-72.ZHANG L,ZHANG C L,LYU J P,WANG C Y,ZHANG Y Y,BU H Y,DU G Z.Effect of light intensity on seed germination in nine boraginaceae species on the eastern Qinghai-Tibet plateau.Journal of Lanzhou University(Natural Sciences),2011,47(5):67-72.
[12]黎彩霞,廖明安,魏志强,沈文,任雅君.不同水温浸种对毛蕊铁线莲种子发芽指标的影响.安徽农业科学,2011,39(30):18479-18480.LI C X,LIAO M A,WEI Z Q,SHEN W,REN Y J.Effects of different water temperature on seeds germination of Clematis lasiandra Maxim.Journal of Anhui Agricultural Sciences,2011,39(30):18479-18480.
[13]孙云波,陈翔,陈垣,周传猛,白德涛.种荚预处理对红芪种子发芽特性的影响.草业学报,2015,24(6):159-167.SUN Y B,CHEN X,CHEN Y,ZHOU C M,BAI D T.Effects of pod pre-treatments on the germination traits of Hedysarum polybotrys seeds.Acta Prataculturae Sinica,2015,24(6):159-167.
[14]黄平权,冯世鑫.萝芙木种子发芽的试验研究.广西中医药大学学报,2012,15(1):61-63.HUANG P Q,FENG S X.Study on the germination of seeds of Rauvolfia verticillata.Journal of Guangxi Traditional Chinese Medical University,2012,15(1):61-63.
[15]张树芬.不同催芽处理及芽苗移栽对云南石梓种子发芽及幼苗生长的影响.种子,2015,34(4):123-125.ZHANG S F.Effects of different acelerating germination treatment and transplanting on seed germination and seedling growth of Koombar.Seed,2015,34(4):123-125.
[16]李鲜花,刘永华,罗彩云,刘翠英,贺英.不同处理对白皮松种子发芽率的影响.榆林学院学报,2014,24(4):21-23.LI X H,LIU Y H,LUO C Y,LIU C Y,HE Y.Influence of different dispositions on germination rate of Pinus bungeana.Journal of Yulin University,2014,24(4):21-23.
[17] NIKOLAEVA M G.Physology of Deep Dormancy in Seeds.Washington,DC:National Science Foundation,1967.
[18]许世泉,贾秀梅,王志清,焉石,张瑞,逢世峰.东北铁线莲种胚后熟发育研究.特产研究,2010,32(4):25-26,38.XU S Q,JIA X M,WANG Z Q,YAN S,ZHANG R,FENG S F.Study on seed embryo postmaturation and development of Clematis mandshurica Rupr.Special Wild Economic Animal and Plant Research,2010,32(4):25-26,38.
[19]刘华,蒋齐,李如来,李明,张清云.不同植物激素对甘草种子萌发的影响.宁夏农林科技,2013,54(2):19-21.LIU H,JIANG Q,LI R L,LI M,ZHANG Q Y.A study of effect of various plant hormones on seed germination of Glycyrrhiza uralensis.Ningxia Journal of Agriculture and Forestry Science and Technology,2013,54(2):19-21.
[20] BIALECKA B.,KEPCZYSKI J.Germination,α-,β-amylase and total dehydrogenase activities of Amaranthus caudatus seeds under water stress in the presence of ethephon or gibberellin A3.Acta Biologica Cracoviensia Series Botanica,2010,52(1):7-12.
[21] DEBEAUJON I,KOORNNEEF M.Gibberellin requirement for Arabidopsis seed germination is determined both by testa characteristics and embryonic abscisic acid.Plant Physiology,2000,122(2):415-424.
[22]许世泉,王振兴,邵财,王志清,逄世峰,张瑞.东北铁线莲种胚后熟过程中内源激素动态变化.特产研究,2011,33(2):37-39.XU S Q,WANG Z X,SHAO C,WANG Z Q,PANG S F,ZHANG R.Morphological development of seed embryo in its postripening and dynamic changes of endogenesis hormone in Clematis mandshurica.Special Wild Economic Animal and Plant Research,2011,33(2):37-39.
[23]邹竣竹,韩蕾,李德颖,孙振元.赤霉素和生长素浸种对野牛草种子萌发及幼苗生长生理的影响草业科学,2017,34(9):1838-1846.ZOU J Z,HAN L,LI D Y,SUN Z Y.Seed soaking in exogenous gibberellin and auxin and their impact on seed germination and seedling physiology of Buchloe dactyloides(Nutt.)Engelm.Pratacultural Science,2017,34(9):1838-1846.
[24]孙丹,张金政,孙国峰,李晓东,何卿.细叶鸢尾种子休眠原因及解除休眠的方法研究.草业学报,2011,20(6):109-117.SUN D,ZHANG J Z,SUN G F,LI X D,HE Q.A study on the causes of Iris tenuifolin dormancy and the methods for breaking dormancy.Acta Prataculturae Sinica,2011,20(6):109-117.
[25]浦新春,韩建国,李敏.结缕草种子脱落酸含量及打破休眠的研究.草地学报,1994,2(1):30-35.PU X C,HAN J G,LI M.Quantity of abscisic acid and break dormancy in Zoysiagrass seed.Acta Agrestia Sinca,1994,2(1):30-35.
[26]邹琇莹,DAVID W FOUNTIN,Ed R MORGAN.赤霉素及机械处理的相互作用对破除Sandersonia aurantiaca种子休眠的研究.草业学报,2003,12(5):70-76.ZOU X Y,DAVID W FOUNTIN,Ed R MORGAN.Seed dormancy of Sandersonia aurantiaca broken by interaction of GA3and mechanical treatment.Acta Prataculturae Sinica,2003,12(5):70-76.
[27]韩云华,王彦荣,陶奇波.种子激素引发.草业科学,2016,33(12):2494-2502.HANY H,WANG Y R,TAO Q B.Advances of seed hormonal priming.Pratacultural Science,2016,33(12):2494-2502.
[28]郑芳昊,潘超美,赖珍珍,夏静,梁钻姬,刘欣.野菊花种子发芽特性的研究.中药材,2012,35(3):351-354.ZHENG F H,PAN C M,LAI Z Z,XIA J,LIANG Z J,LIU X.Study on germination characteristic of seed of Dendranthema indicum.Journal of Chinese Medicinal Materials,2012,35(3):351-354.
[29]焦德志,龚孟,潘学岩,朱蕾.不同植物激素对羊草种子萌发和幼苗生长的影响.安徽农业科学,2010,38(3):1188-1190.JIAO D Z,GONG M,PAN X Y,ZHU L.Effects of various phytohormones on germination and growth of L.chinensis seeds.Journal of Anhui Agricultural Sciences,2010,38(3):1188-1190.
[30]王磊,周余华,关雪莲,张虎,陈少卿.GA3和6-BA对粉绿铁线莲种子发芽特性的影响.种子,2010,29(3):44-50.WANG L,ZHOU Y H,GUAN X L,ZHANG H,CHEN S Q.Effects of plant growth regulators GA3and 6-BA on seed germinaion characteristics of Clematis glauca.Seed,2010,29(3):44-50.
[31]刘志高,季梦成,杨彦鹏,沈永宝.3种铁线莲属植物种子萌发特性研究.种子,2015,34(6):30-33.LIU Z G,JI M C,YANG Y P,SHEN Y B.Study on germination characteristics of three species from Clematis.Seed,2015,34(6):30-33.
[32]王娜,王奎玲,刘庆华,刘庆超.三种铁线莲种子萌发研究.2016年中国观赏园艺学术研讨会论文集.长沙:中国园艺学会,2016:492-495.WANG N,WANG K L,LIU Q H,LIU Q C.Study on the germination characteristics of three Clematis.Proceedings of 2016China Ornamental Horticulture Academic Seminar.Changsha:Chinese Society for Horticultural Science,2016:492-495.
[33]王非,王金侠,李强,何淼.GA3和IAA处理对4种铁线莲种子萌发的影响.草业科学,2014,31(4):672-676.WANG F,WANG J X,LI Q,HE M.Effects of GA3and IAA on the germination of four species of Clematis seeds.Pratacultural Science,2014,31(4):672-676.