缢蛏EGFR基因内含子1内SNP位点多态性与生长性状相关性
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摘要
为研究缢蛏表皮生长因子受体基因(epidermal growth factor receptor,EGFR)单核苷酸多态性(single nucleotide polymorphism, SNP)与生长性状(壳长、壳宽、壳高和体质量)的相关性。本实验利用直接测序法从缢蛏EGFR基因的第一个内含子序列中共筛选到17个SNP位点。卡方检验结果显示,在17个位点中,有13个位点符合Hardy-Weinberg平衡,位点多态性检测显示17个位点中有10个位点表现为中等多态性(0.25 In order to study the correlation between EGFR gene and growth traits(shell length, shell width, shell height and body weight), we analyzed the variation of EGFR intron 1 sequence from Sinonovacula constricta by using direct sequencing. There were 17 SNP sites in intron 1, named SNP1–SNP17 respectively. Based on Chisquare test, the 13 sites were fitted to Hardy-Weinberg equilibrium, and the 10 sites showed moderate polymorphism through polymorphism detection(0.25
引文
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[25]Vignal A,Milan D,SanCristobal M,et al.A review on SNP and other types of molecular markers and their use in animal genetics[J].Genetics Selection Evolution,2002,34(3):275-305.
[26]Zhao Z M,Fu Y X,Hewett-Emmett D,et al.Investigating single nucleotide polymorphism(SNP)density in the human genome and its implications for molecular evolution[J].Gene,2003,312:207-213.
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[28]Brookes A J.The essence of SNPs[J].Gene,1999,234(2):177-186.
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[30]Tao W J,Boulding E G.Associations between single nucleotide polymorphisms in candidate genes and growth rate in Arctic charr(Salvelinus alpinus L.)[J].Heredity,2003,91(1):60-69.
[31]Drysdale C M,McGraw D W,Stack C B,et al.Complex promoter and coding regionβ2-adrenergic receptor haplotypes alter receptor expression and predict in vivo responsiveness[J].Proceedings of the National Academy of Sciences of the United States of America,2000,97(19):10483-10488.
[2]Michelmore R W,Paran I,Kesseli R V.Identification of markers linked to disease-resistance genes by bulked segregant analysis:a rapid method to detect markers in specific genomic regions by using segregating populations[J].Proceedings of the National Academy of Sciences of the United States of America,1991,88(21):9828-9832.
[3]刘晓慧,王义权.分子标记技术在水产动物研究中的应用[J].厦门大学学报(自然科学版),2008,47(S2):228-231.Liu X H,Wang Y X.The applications of molecular marker in the study on aquatic animals[J].Journal of Xiamen University(Natural Science Edition),2008,47(S2):228-231(in Chinese).
[4]谭新,童金苟.SNPs及其在水产动物遗传学与育种学研究中的应用[J].水生生物学报,2011,35(2):348-354.Tan X,Tong J G.SNPs and their applications in studies on genetics and breeding of aquaculture animals[J].Acta Hydrobiologica Sinica,2011,35(2):348-354(in Chinese).
[5]Syv?nen A C.Accessing genetic variation:genotyping single nucleotide polymorphisms[J].Nature Reviews Genetics,2001,2(12):930-942.
[6]谢淑媚,牛东红,阮海灯,等.缢蛏IGFBP基因结构及生长性状相关SNP筛选[J].水产学报,2015,39(6):799-809.Xie S M,Niu D H,Ruan H D,et al.Molecular characterization of IGFBP and association analysis with growth traits in the razor clam Sinonovacula constricta[J].Journal of Fisheries of China,2015,39(6):799-809(in Chinese).
[7]Niu D H,Wang L,Bai Z Y,et al.Identification and expression characterization of the Myostatin(MSTN)gene and association analysis with growth traits in the razor clam Sinonovacula constricta[J].Gene,2015,555(2):297-304.
[8]韩学凯,陈夏君,白志毅,等.三角帆蚌HcTyr基因内壳色性状相关SNP筛选及图谱定位[J].水产学报,2017,41(7):1044-1053.Han X K,Chen X J,Bai Z Y,et al.Detection of shell nacre colour-related SNP and gene mapping of HcTyr gene in Hyriopsis cumingii[J].Journal of Fisheries of China,2017,41(7):1044-1053(in Chinese).
[9]Song Z,Fusco J,Zimmerman R,et al.Epidermal growth factor receptor signaling regulatesβcell proliferation in adult mice[J].Journal of Biological Chemistry,2016,291(43):22630-22637.
[10]Zhou X J,Zhang W,Yao Q S,et al.Exosome production and its regulation of EGFR during wound healing in renal tubular cells[J].American Journal of PhysiologyRenal Physiology,2017,312(6):F963-F970.
[11]Kalo?lu C,Bulut H E,Onarlio?lu B,et al.Epidermal growth factor receptor(EGFR)immunolocalization in the male rat reproductive tract during pre-and postnatal periods[J].Turkish Journal of Veterinary&Animal Sciences,2000,24(5):501-509.
[12]Jou Y S,Lo Y L,Hsiao C F,et al.Association of an EGFR intron 1 SNP with never-smoking female lung adenocarcinoma patients[J].Lung Cancer,2009,64(3):251-256.
[13]孙静哲,胡妮娅,易金萍,等.EGFR基因rs763317多态性与胃癌遗传易感的相关性研究[J].中华肿瘤防治杂志,2010,17(10):724-727.Sun J Z,Hu N Y,Yi J P,et al.Relationship of EGFRgene rs763317 single nucleotide polymorphism with genetic susceptibility of gastric cancer[J].Chinese Journal of Cancer Prevention and Treatment,2010,17(10):724-727(in Chinese).
[14]李霞,王雪,秦艳杰,等.仿刺参EGFR基因的克隆与表达分析[J].水产学报,2012,36(1):41-49.Li X,Wang X,Qin Y J,et al.Cloning and expression analysis of the EGFR gene in Apostichopus japonicus[J].Journal of Fisheries of China,2012,36(1):41-49(in Chinese).
[15]Sharabi O,Ventura T,Manor R,et al.Epidermal growth factor receptor in the prawn Macrobrachium rosenbergii:function and putative signaling cascade[J].Endocrinology,2013,154(9):3188-3196.
[16]Dabour N,Bando T,Nakamura T,et al.Cricket body size is altered by systemic RNAi against insulin signaling components and epidermal growth factor receptor[J].Development,Growth&Differentiation,2011,53(7):857-869.
[17]van Kesteren R E,Gagatek J S,Hagendorf A,et al.Postsynaptic expression of an epidermal growth factor receptor regulates cholinergic synapse formation between identified molluscan neurons[J].European Journal of Neuroscience,2008,27(8):2043-2056.
[18]Hall T A.BioEdit:a user-friendly biological sequence alignment editor and analysis program for Windows95/98/NT[J].Nucleic Acids Symposium Series,1999,41:95-98.
[19]Shi Y Y,He L.SHEsis,a powerful software platform for analyses of linkage disequilibrium,haplotype construction,and genetic association at polymorphism loci[J].Cell Research,2006,16(10):851.
[20]Kalinowski S T,Taper M L,Marshall T C.Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment[J].Molecular Ecology,2007,16(5):1099-1106.
[21]Coakes S J,Steed L.SPSS:Analysis without Anguish Using SPSS Version 14.0 for Windows[M].New York:John Wiley&Sons,Inc.,2009.
[22]Botstein D,White R L,Skolnick M,et al.Construction of a genetic linkage map in man using restriction fragment length polymorphisms[J].American Journal of Human Genetics,1980,32(3):314-331.
[23]Bishayee S.Role of conformational alteration in the epidermal growth factor receptor(EGFR)function[J].Biochemical Pharmacology,2000,60(8):1217-1223.
[24]Sun L L,Huan P,Wang H X,et al.An EGFR gene of the Pacific oyster Crassostrea gigas functions in wound healing and promotes cell proliferation[J].Molecular Biology Reports,2014,41(5):2757-2765.
[25]Vignal A,Milan D,SanCristobal M,et al.A review on SNP and other types of molecular markers and their use in animal genetics[J].Genetics Selection Evolution,2002,34(3):275-305.
[26]Zhao Z M,Fu Y X,Hewett-Emmett D,et al.Investigating single nucleotide polymorphism(SNP)density in the human genome and its implications for molecular evolution[J].Gene,2003,312:207-213.
[27]桂建芳,朱作言.水产动物重要经济性状的分子基础及其遗传改良[J].科学通报,2012,57(15):1751-1760.Gui J F,Zhu Z Y.Molecular basis and genetic improvement of economically important traits in aquaculture animals[J].Chinese Science Bulletin,2012,57(15):1751-1760.
[28]Brookes A J.The essence of SNPs[J].Gene,1999,234(2):177-186.
[29]De-Santis C,Jerry D R.Candidate growth genes in finfish-Where should we be looking?[J].Aquaculture,2007,272(1-4):22-38.
[30]Tao W J,Boulding E G.Associations between single nucleotide polymorphisms in candidate genes and growth rate in Arctic charr(Salvelinus alpinus L.)[J].Heredity,2003,91(1):60-69.
[31]Drysdale C M,McGraw D W,Stack C B,et al.Complex promoter and coding regionβ2-adrenergic receptor haplotypes alter receptor expression and predict in vivo responsiveness[J].Proceedings of the National Academy of Sciences of the United States of America,2000,97(19):10483-10488.