一株沙雷氏菌的分离鉴定及其对德国小蠊的毒力测定
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摘要
从自然染病死亡的中华真地鳖(Eupolyphaga sinensis Walker)虫尸中分离到一株产紫红色色素的昆虫病原菌EB0732,经生理生化指标和16S rDNA分子生物学鉴定,该菌株为粘质沙雷氏菌(Serratia marcescens EB0732)。为探究该菌株对德国小蠊的毒杀效果,选取5个浓度(1×10~5CFU/mL、1×10~6CFU/mL、1×10~7CFU/mL、1×10~8CFU/mL、1×10~9CFU/mL)对不同龄期的德国小蠊(1～3龄若虫,4～5龄若虫,6～7龄雌、雄若虫,雌、雄成虫)进行了毒力测定。结果显示,该菌株对不同龄期德国小蠊均具有较强毒力,1×10~9 CFU/mL悬液对德国小蠊1～3龄若虫20 d累计杀灭率高达87.50%,雌、雄成虫分别达到31.67%、33.33%,对德国小蠊的防治具有较大的潜在应用价值。
引文
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[3]赵晓峰.粘质沙雷氏菌PS-1菌株对甜菜夜蛾幼虫杀虫机理的初步研究.广州:华南农业大学硕士学位论文,2016.
[4]金虹,葛绍荣,陶勇,等.一株蝗虫病原菌的鉴定及其毒力和病理研究.微生物学报,2005,45(2):172.
[5]万秀秀.粘质沙雷氏菌对褐飞虱的亚致死效应.南京:南京农业大学硕士学位论文,2015.
[6]邓彩萍.光肩星天牛刻槽微生物及其对幼虫致病性的研究.北京:北京林业大学博士学位论文,2009.
[7]冯书亮,曹伟平,范秀华,等.一株粘质沙雷氏菌菌株的鉴定及对黄胫小车蝗的毒力测定.中国生物防治学报,2002,18(4):158.
[8]杨建云,曹溪,纪春艳,等.粘质沙雷氏菌菌株PS-1对甜菜夜蛾种群增长的抑制作用.中国生物防治学报,2015,31(4):501.
[9]林加涵,魏文铃,彭宣宪.现代生物学实验(上册).北京:高等教育出版社,2000:70.
[10]陈秀为,兰洪霞.粘质沙雷氏菌对棉铃虫、菜青虫致病力及田间控制的研究初报.天津农学院学报,2001,8(3):28.
[11] Gutierrez A C, Goíbiowski M, Pennisi M, et al. Cuticle fatty acid composition and differential susceptibility of three species of cockroaches to the entomopathogenic fungi Metarhizium anisopliae(Ascomycota, Hypocreales). Journal of Economic Entomology, 2015,108(2):752.
[12] Pardo S, Martínez A M, Figueroa J I, et al. Insecticide resistance of adults and nymphs of Asian citrus psyllid populations from Apatzingán Valley, Mexico. Pest Management Science,2018,74(1):34.
[13] Lopes R B, Alves S B. Differential susceptibility of adults and nymphs of Blattella germanica(L.)(Blattodea:Blattellidae)to infection by Metarhizium anisopliae and assessment of delivery strategies. Neotropical Entomology, 2011, 40(3):368.
[14]李洪波,杜巍,赵凯,周东坡.灵菌红素研究进展.生物技术通讯,2011,22(1):139.
[15] Harris A K, Williamson N R, Slater H, et al. The Serratia gene cluster encoding biosynthesis of the red antibiotic,prodigiosin, shows species-and strain-dependent genome context variation. Microbiology, 2004,150(Pt 11):3547.
[16]刘敬瑞.嗜线虫沙雷氏菌菌株DZ0503SBS1杀虫活性相关生物学特征及杀虫毒素蛋白的分离纯化.南京:南京农业大学硕士学位论文,2009.