12周龄大围山微型鸡与艾维茵肉鸡肌肉营养成分及LPL基因表达差异研究
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摘要
为研究云南地方鸡种大围山微型鸡与艾维茵肉鸡肌肉营养成分中粗脂肪含量与脂蛋白脂酶(LPL)基因mRNA表达差异,屠宰同批12周龄大围山微型鸡和艾维茵肉鸡各20只,测定肌肉营养成分含量和LPL基因表达量。结果显示:在胸肌和腿肌中,大围山微型鸡粗灰分含量、粗蛋白含量、粗脂肪含量均显著高于艾维茵肉鸡,在胸肌和腿肌中大围山微型鸡水分的含量显著低于艾维茵肉鸡,且胸肌和腿肌中大围山微型鸡LPL基因表达量分别为3.98、5.72,均高于艾维茵肉鸡的3.70、3.54。可见,大围山微型鸡肌肉粗蛋白、粗脂肪等营养成分优于艾维茵肉鸡,LPL基因促进脂肪沉积,因此LPL基因可作为影响家禽脂肪沉积的重要候选基因。
In order to study the differences of muscle nutrient composition and LPL gene expression in Daweishan mini chicken and Avian broiler, 20 Daweishan mini chickens and 20 Avian broilers were slaughtered at 12 weeks of age, and muscle nutrient content and LPL gene expression were measured. The results showed that, in breast muscle and leg muscle the crude ash content, crude protein content and crude fat content of Daweishan mini chickens were significantly higher than that of Avian broilers. However, the moisture content of Daweishan mini chickens in breast muscle and leg muscle were significantly lower than that of Avian broilers. The LPL gene expression in breast and leg muscle of Daweishan mini chickens was 3.98 and 5.72, both higher than that of Avian broilers(3.70 and 3.54). Daweishan mini chicken showed better muscle crude protein, crude fat and other nutrients than Avian broiler, and LPL gene could promote fat deposition in muscle. So LPL gene can be used as an important candidate gene to affect fat deposition in poultry.
In order to study the differences of muscle nutrient composition and LPL gene expression in Daweishan mini chicken and Avian broiler, 20 Daweishan mini chickens and 20 Avian broilers were slaughtered at 12 weeks of age, and muscle nutrient content and LPL gene expression were measured. The results showed that, in breast muscle and leg muscle the crude ash content, crude protein content and crude fat content of Daweishan mini chickens were significantly higher than that of Avian broilers. However, the moisture content of Daweishan mini chickens in breast muscle and leg muscle were significantly lower than that of Avian broilers. The LPL gene expression in breast and leg muscle of Daweishan mini chickens was 3.98 and 5.72, both higher than that of Avian broilers(3.70 and 3.54). Daweishan mini chicken showed better muscle crude protein, crude fat and other nutrients than Avian broiler, and LPL gene could promote fat deposition in muscle. So LPL gene can be used as an important candidate gene to affect fat deposition in poultry.
引文
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[3]李刚,谷大海,徐志强,等.大围山微型鸡与武定鸡屠宰性能比较分析[J].黑龙江畜牧兽医,2015(10):41-42.
[4]食品安全国家标准食品中水分的测定:GB5009.3-2010[S].2010.
[5]食品安全国家标准食品中脂肪的测定:GB5009.6-2016[S].2016.
[6]食品安全国家标准食品中蛋白质的测定:GB5009.5-2010[S].2010.
[7]食品安全国家标准食品中灰分的测定:GB5009.4-2010[S].2010.
[8]孟庆辉.不同粗蛋白水平对樱桃谷肉鸭生产性能的影响[J].西昌学院学报(自然科学版),2011(3):20-23.
[9]朱丹实,吴晓菲,刘贺,等.水分对生鲜肉品品质的影响[J].食品工业科技,2013,34(16):363-366.
[10]舒婷,沙尔山别克·阿不地力大,姜维,等.拜城油鸡肌内脂肪沉积规律及其与肉品质的关联性分析[J].中国家禽,2018,40(6):5-9.
[11]庄柯瑾,王帅,王锡昌,等.饲料中脂肪对水产品品质影响的研究进展[J].食品科学,2015,36(15):288-292.
[12]申杰,潘爱銮,蒲跃进,等.不同品种鸡胸肌脂肪酸组成分析[J].湖北农业科学,2014,53(23):5805-5808.
[13]赵建国,梁伟放,柳贤德.海南文昌鸡和AA鸡的肉品特性比较研究[J].热带农业工程,2017,41(2):84-86.
[14]王志祥,马秋刚,关舒,等.地方鸡种固始鸡与快大型肉鸡肉质性状的比较研究[J].中国农业大学学报,2005(3):48-51.
[15]潘爱銮,皮劲松,梁振华,等.6个品种鸡LPL基因的PCR-RFLP分析[J].中国家禽,2004(S1):158-160.
[16]AUWERX J,LEROY P,SCHOONJANS K.Lipoprotein lipase:Recent contributions from molecular biology[J].Critical Reviews in Clinical Laboratory Sciences,1992,29(3-4):243-268.
[17]王晶.广西三黄鸡肉质性状分析及LPL、H-FABP基因表达与肌内脂肪含量的相关研究[D].南宁:广西大学,2013.
[18]乔永,黄治国,李齐发,等.绵羊肌肉LPL基因表达的发育性变化及其对肌内脂肪含量的影响[J].中国农业科学,2007(10):2323-2330.
[19]杨恒东,王梦芝,宋莉,等.兴义矮脚鸡屠宰性能、肌肉品质及LPL基因表达的研究[J].中国畜牧杂志,2009,45(13):12-15.
[20]刘蒙,宋代军,齐珂珂,等.日粮代谢能水平对北京油鸡脂肪沉积和LPL基因表达的影响[J].中国畜牧兽医,2009,36(5):9-13.