腐乳毛霉醇提物不同极性部位抗氧化活性及细胞保护作用研究
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摘要
目的:观察腐乳毛霉醇提物不同极性部位抗氧化活性及对L-O2细胞保护作用.方法:分别将腐乳毛霉醇提物萃取后的石油醚、乙酸乙酯、正丁醇及水部位进行体外抗氧化活性和对CCl_4致LO2细胞损伤的保护实验.结果:腐乳毛霉醇提物均具有较强的抗氧化、清除DPPH和ABTS自由基及FRAP能力,对CCl_4引起L-O2细胞的氧化损伤具有显著的保护作用,可降低细胞内ROS及培养液中MDA含量和LDH活性,升高SOD、GSH-Px和CAT活性,从而减轻CCl_4对L-O2细胞氧化损伤,其作用效果由高到低依次是乙酸乙酯、正丁醇、水和石油醚部位.结论:腐乳毛霉醇提物不同极性部位对CCl_4引起L-O2细胞的氧化损伤具有显著的保护作用,其作用机制可能与抗氧化作用有关.
The aim is to observe the antioxidant activities of different polar parts of ethanol extract of Mucor Sufu and their protective effects on carbon tetrachloride(CCl_4)induced oxidative stress in L-O2 cells.The petroleum ether,ethyl acetate,n-butanol and water parts come from ethanol extract of Mucor Sufu are taken respectively to do protective tests,and then to assess their antioxidant activities and their cytoprotective effects on CCl_4 damaged L-O2 cells.The results show that the aforementioned four parts had strong antioxidant,scavenging DPPH and ABTS free radicals and FRAP,and total antioxidant capacities.They possessed significantly cyprotective effects on CCl_4 damaged L-O2 cells,which could reduce the LDH activity,MDA content in culture medium and intracellular ROS concentration,increase SOD,GSH-Px and CAT activities in the culture medium,thereby reduce the L-O2 cell oxidative damage.Their effects in turn from high to low were ethyl acetate,n-butanol,water and petroleum ether parts.Finally,itisconcludedthatthe different polar parts of the ethanol extracts of Mucor Sufu have a significantly cyprotective effect on the L-O2 cell oxidative damage induced by CCl_4;and their mechanisms may be related to the antioxidant activity.
The aim is to observe the antioxidant activities of different polar parts of ethanol extract of Mucor Sufu and their protective effects on carbon tetrachloride(CCl_4)induced oxidative stress in L-O2 cells.The petroleum ether,ethyl acetate,n-butanol and water parts come from ethanol extract of Mucor Sufu are taken respectively to do protective tests,and then to assess their antioxidant activities and their cytoprotective effects on CCl_4 damaged L-O2 cells.The results show that the aforementioned four parts had strong antioxidant,scavenging DPPH and ABTS free radicals and FRAP,and total antioxidant capacities.They possessed significantly cyprotective effects on CCl_4 damaged L-O2 cells,which could reduce the LDH activity,MDA content in culture medium and intracellular ROS concentration,increase SOD,GSH-Px and CAT activities in the culture medium,thereby reduce the L-O2 cell oxidative damage.Their effects in turn from high to low were ethyl acetate,n-butanol,water and petroleum ether parts.Finally,itisconcludedthatthe different polar parts of the ethanol extracts of Mucor Sufu have a significantly cyprotective effect on the L-O2 cell oxidative damage induced by CCl_4;and their mechanisms may be related to the antioxidant activity.
引文
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[2] Lee Y J,Moon M K,Hwang S M,et al.Anti-inflammatory Effect of Buddleja Officinalis on Vascular Inflammation in Human Umbilical Vein Endothelial Cells[J].Am J Chin Med,2010,38(3):585-598.
[3] Dehghan H,Sarrafi Y,Salehi P.Antioxidant and Antidiabetic Activities of 11 Herbal Plants from Hyrcania Region,Iran[J].J Food Drug Anal,2016,24(1):179-188.
[4] Mortezaee K,Khanlarkhani N.Melatonin Application in Targeting Oxidative-induced Liver Injuries:A Review[J].J Cell Physiol,2018,233(5):4015-4032.