温度对嘉陵江鲇消化酶活性影响的研究
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摘要
采用酶学分析方法研究了温度对嘉陵江鲇胃、前肠、中肠、后肠和肝胰脏中蛋白酶、淀粉酶和脂肪酶活性的影响.结果表明:在设定的温度范围内,鲇鱼各消化酶的活性均随着温度的升高呈现出先升后降的变化趋势.鲇胃、肠道和肝胰脏中蛋白酶最适温度分别为40℃,44℃和36℃,淀粉酶的最适温度分别为44℃,40℃和40℃,脂肪酶的最适温度均为36℃;在最适温度下,蛋白酶活性从大到小依次为前肠、肝胰脏、胃、中肠、后肠,淀粉酶的活力从大到小依次为肝胰脏、胃、前肠、中肠、后肠,脂肪酶的活力从大到小依次为肝胰脏、胃、前肠、中肠、后肠,且各部位之间差异显著(p<0.05),说明嘉陵江鲇的各种消化酶活性存在着器官特异性.
An experiment was conducted to study the effect of temperature on the activities of digestive enzymes in stomach,intestine and hepatopancreas of Silurus asotus by means of enzymology analysis.The results showed that within the range of the designed temperature the activities of all digestive enzymes in S.asotus increased at first and then decreased.The optimal temperature was 40 ℃,44 ℃ and 36 ℃,respectively,for protease and 44 ℃,40 ℃and 40 ℃,respectively,for amylase in the stomach,the intestine and the hepatopancreas of the fish.The optimum temperature for lipase in all the organs studied was 36 ℃.Under the optimum temperature,the activity of protease in different organs was in the order of anterior intestine>hepatopancreas>stomach>middle intestine>posterior intestine,and the activity of both amylase and lipase was in the order of hepatopancreas>stomach>anterior intestine>middle intestine>posterior intestine.Significant differences(p<0.05) existed among different digestive organs at the same temperature,indicating that the activities of digestive enzymes in S.asotus are organ-specific.
An experiment was conducted to study the effect of temperature on the activities of digestive enzymes in stomach,intestine and hepatopancreas of Silurus asotus by means of enzymology analysis.The results showed that within the range of the designed temperature the activities of all digestive enzymes in S.asotus increased at first and then decreased.The optimal temperature was 40 ℃,44 ℃ and 36 ℃,respectively,for protease and 44 ℃,40 ℃and 40 ℃,respectively,for amylase in the stomach,the intestine and the hepatopancreas of the fish.The optimum temperature for lipase in all the organs studied was 36 ℃.Under the optimum temperature,the activity of protease in different organs was in the order of anterior intestine>hepatopancreas>stomach>middle intestine>posterior intestine,and the activity of both amylase and lipase was in the order of hepatopancreas>stomach>anterior intestine>middle intestine>posterior intestine.Significant differences(p<0.05) existed among different digestive organs at the same temperature,indicating that the activities of digestive enzymes in S.asotus are organ-specific.
引文
[1]叶元土,林仕梅,罗莉,等.温度、pH值对南方大口鲶、长吻鮠蛋白酶和淀粉酶活力的影响[J].大连水产学院学报,1998,13(2):17-23.
[2]叶继丹,卢彤岩,刘洪柏,等.六种鲟鱼消化酶活性的比较研究[J].水生生物学报,2003,27(6):590-594.
[3]毕冰,孙中武,肖晓文,等.鲤、鲢、鳙、草鱼消化道消化酶种类和活性的比较研究[J].水产学杂志,2011,24(2):17-20.
[4]吴婷婷,朱晓鸣.鳜鱼、青鱼、草鱼、鲤、鲫、鲢消化酶活性的研究[J].中国水产科学,1994,1(2):11-17.
[5]罗奇,区又君,艾丽,等.温度和pH对条石鲷幼鱼消化酶活力的影响[J].热带海洋学报,2010,29(5):154-158.
[6]HOFER R.The Adaptation of Digestive Enzymes to Temperature,Seasons and Diet in Roach,Rutilus rutilus L.and Rudd Scardinius erythrophthalmus L.Ⅰ:Amylase[J].J Fish Biol,1978,14(6):565-572.
[7]HOFER R.The Adaptation of Digestive Enzymes to Temperature,Seasons and Diet in Roach,Rutilus rutilus L.and Rudd Scardinius erythrophthalmus L.Ⅱ:Protease[J].J Fish Biol,1979,15(4):373-379.
[8]丁瑞华.四川鱼类志[M].成都:四川科技出版社,1994:444-447.
[9]向枭,叶元土,周兴华,等.鲇的消化能力与营养价值分析[J].动物学杂志,2004,39(6):65-69.
[10]潘庭双,胡贤江,蒋叶林,等.野生鲶生物学特性及亲鱼食性驯化技术[J].水利渔业,2003,23(1):39.
[11]马旭洲,崔存河,王志远,等.鲶鱼人工繁殖及苗种培育技术[J].水产养殖,2002,5(5):19-24.
[12]向枭,叶元土,周兴华,等.鲇胃肠道、胰脏对7种饲料蛋白质的酶解动力学[J].水生生物学报,2006,36(4):493-498.
[13]中山大学生物系.生化技术导论[M].北京:科学出版社,1979:42-54.
[14]叶元土,薛敏,林仕梅,等.草鱼肠道、肝胰脏对饲料蛋白质酶解速度的比较[J].中国水产科学,2003,10(2):173-176.
[15]JOHN E H.Fish Nutrition[M].California:Academic Press Incorporated,1987:332-423.
[16]伍莉,蒋莉,陈鹏飞.温度对黄鳝肠道和肝胰脏主要消化酶活力的影响[J].四川畜牧兽医,2003,30(10):26-27.
[17]桂远明,吴垠,刘焕亮,等.温度对草鱼、鲤、鲢、鳙主要消化酶活性的影响[J].大连水产学院学报,1993,8(4):1-8.
[18]李希国,李加儿,区又君.温度对黄鳍鲷主要消化酶活性的影响[J].南方水产,2006,2(1):43-48.
[19]阮国良,杨代勤,严安生.不同温度对月鳢主要消化酶活性的影响[J].长江大学学报:自然科学版,2008,5(4):48-51.
[20]杨蕙萍,童圣英,王子臣.国内外关于水产动物消化酶研究的概况[J].大连水产学院学报,1998,13(3):64-71.
[21]柳劲松,吕建伟,彭巨亮.外温动物的内温性[J].高师理科学刊,2000,20(4):48-50.
[22]LOVETT D L,FLDER D L.Ontogenetic Change in Digestive Enzyme Activity of Larval and Postlarval White Shrimp Penaeus Setiferus[J].Biological Bulletin,1990,178(2):144-159.
[23]尾崎久雄.鱼类消化生理(下册)[M].吴尚忠,译.上海:上海科学技术出版社,1985:390-398.
[24]马燕梅,梅景良,林树根.鳜胃肠道和肝脏主要消化酶活性的研究[J].江西农业大学学报,2004,26(4):584-588.
[25]韩庆,刘良国,张建平,等.温度和pH对洞庭鲇鱼消化酶活性的影响[J].水生生物学报,2011,35(1):22-29.
[26]周景祥,余涛,黄权,等.鲤鱼、黄颡鱼和大眼鰤鲈消化酶活性的比较研究[J].吉林农业大学学报,2001,23(1):94-96.
[27]CHESLEY L C.The Concentrations of Proteases,Amylase,and Lipase in Certain Marine Fishes[J].Biological Bulletin,1934,66(14):133-144.
[28]寿建昕,沈文英,吴颖芳,等.温度对翘嘴红鲌消化酶活力的影响[J].水利渔业,2006,26(2):6-7,25.
[29]梅景良,马燕梅.温度和pH对黑鲷主要消化酶活性的影响[J].集美大学学报:自然科学版,2004,9(3):226-230.
[30]付新华,孙谧,孙世春.大菱鲆消化酶的活力[J].中国水产科学,2005,12(1):26-32.
[31]李芹,唐洪玉.中华倒刺鲃仔稚鱼期消化酶及碱性磷酸酶活性变化的研究[J].水生态学杂志,2010,3(5):82-85.
[32]沈文英,祝尧荣,钱科亮.温度和pH对澳洲宝石鱼消化酶活性的影响[J].大连水产学院学报,2006,21(2):189-192.
[33]杨元昊,周继术,吉红,等.温度对兰州鲇消化酶活性的影响[J].动物学杂志,2006,41(6):104-108.
[34]胡毅,潘鲁青.10种金属离子对三梭子蟹中肠腺消化酶活性的影响[J].热带海洋学报,2006,25(6):52-57.
[35]黄瑾,熊邦喜,陈洁,等.鱼类消化酶活性与其体长、体重和水质的相关性研究[J].水生态学杂志,2012,33(2):121-126.
[36]FANG L S,CHIOU S F.Effect of Salinity on the Activities of Digestive Proteases from the Tilapia Fish,Oreochromis Niloticus in Different Culture Environments[J].Comparative Biochemistry and Physiology,1989,93A(2):439-443.
[37]戴家银,郑微云.铜和锌离子对真鲷幼鱼组织酶活性的影响[J].环境科学,1998,l9(5):60-62.
[38]江信红,唐云明.黄鳝肠道蛋白酶的应用研究[J].西南师范大学学报:自然科学版,2006,31(4):143~147.
[39]祝云龙,李辉信,韩士群,等.氮磷对滩塘养殖水体及浮游植物的影响[J].江苏农业科学,2004(5):92-95.
[40]高梅,罗毅平,曹振东,等.饲料碳水化合物对南方鲇(Silurus meridionalis Chen)幼鱼消化酶活性的影响[J].西南师范大学学报:自然科学版,2006,31(2):119-123.
[41]邹师哲,王义强,张家圆.饲料中蛋白质、脂肪、碳水化合物对鲤消化酶的影响[J].上海水产大学学报,1998,7(1):69-74.
[42]NAGASE G.Contribution to the Physiology of Digestion in Tilapia Mossambica Peters:Digestive Enzymes and the Effects of Diets on Their Activity[J].Zeitschrift Fürvergleichende Physiologie,1964,49(3):270-284.
[43]李广丽,王义强.草鱼、鲤肠道,肝胰脏消化酶活性的初步研究[J].湛江水产学院学报,1994,14(1):34-40.
[2]叶继丹,卢彤岩,刘洪柏,等.六种鲟鱼消化酶活性的比较研究[J].水生生物学报,2003,27(6):590-594.
[3]毕冰,孙中武,肖晓文,等.鲤、鲢、鳙、草鱼消化道消化酶种类和活性的比较研究[J].水产学杂志,2011,24(2):17-20.
[4]吴婷婷,朱晓鸣.鳜鱼、青鱼、草鱼、鲤、鲫、鲢消化酶活性的研究[J].中国水产科学,1994,1(2):11-17.
[5]罗奇,区又君,艾丽,等.温度和pH对条石鲷幼鱼消化酶活力的影响[J].热带海洋学报,2010,29(5):154-158.
[6]HOFER R.The Adaptation of Digestive Enzymes to Temperature,Seasons and Diet in Roach,Rutilus rutilus L.and Rudd Scardinius erythrophthalmus L.Ⅰ:Amylase[J].J Fish Biol,1978,14(6):565-572.
[7]HOFER R.The Adaptation of Digestive Enzymes to Temperature,Seasons and Diet in Roach,Rutilus rutilus L.and Rudd Scardinius erythrophthalmus L.Ⅱ:Protease[J].J Fish Biol,1979,15(4):373-379.
[8]丁瑞华.四川鱼类志[M].成都:四川科技出版社,1994:444-447.
[9]向枭,叶元土,周兴华,等.鲇的消化能力与营养价值分析[J].动物学杂志,2004,39(6):65-69.
[10]潘庭双,胡贤江,蒋叶林,等.野生鲶生物学特性及亲鱼食性驯化技术[J].水利渔业,2003,23(1):39.
[11]马旭洲,崔存河,王志远,等.鲶鱼人工繁殖及苗种培育技术[J].水产养殖,2002,5(5):19-24.
[12]向枭,叶元土,周兴华,等.鲇胃肠道、胰脏对7种饲料蛋白质的酶解动力学[J].水生生物学报,2006,36(4):493-498.
[13]中山大学生物系.生化技术导论[M].北京:科学出版社,1979:42-54.
[14]叶元土,薛敏,林仕梅,等.草鱼肠道、肝胰脏对饲料蛋白质酶解速度的比较[J].中国水产科学,2003,10(2):173-176.
[15]JOHN E H.Fish Nutrition[M].California:Academic Press Incorporated,1987:332-423.
[16]伍莉,蒋莉,陈鹏飞.温度对黄鳝肠道和肝胰脏主要消化酶活力的影响[J].四川畜牧兽医,2003,30(10):26-27.
[17]桂远明,吴垠,刘焕亮,等.温度对草鱼、鲤、鲢、鳙主要消化酶活性的影响[J].大连水产学院学报,1993,8(4):1-8.
[18]李希国,李加儿,区又君.温度对黄鳍鲷主要消化酶活性的影响[J].南方水产,2006,2(1):43-48.
[19]阮国良,杨代勤,严安生.不同温度对月鳢主要消化酶活性的影响[J].长江大学学报:自然科学版,2008,5(4):48-51.
[20]杨蕙萍,童圣英,王子臣.国内外关于水产动物消化酶研究的概况[J].大连水产学院学报,1998,13(3):64-71.
[21]柳劲松,吕建伟,彭巨亮.外温动物的内温性[J].高师理科学刊,2000,20(4):48-50.
[22]LOVETT D L,FLDER D L.Ontogenetic Change in Digestive Enzyme Activity of Larval and Postlarval White Shrimp Penaeus Setiferus[J].Biological Bulletin,1990,178(2):144-159.
[23]尾崎久雄.鱼类消化生理(下册)[M].吴尚忠,译.上海:上海科学技术出版社,1985:390-398.
[24]马燕梅,梅景良,林树根.鳜胃肠道和肝脏主要消化酶活性的研究[J].江西农业大学学报,2004,26(4):584-588.
[25]韩庆,刘良国,张建平,等.温度和pH对洞庭鲇鱼消化酶活性的影响[J].水生生物学报,2011,35(1):22-29.
[26]周景祥,余涛,黄权,等.鲤鱼、黄颡鱼和大眼鰤鲈消化酶活性的比较研究[J].吉林农业大学学报,2001,23(1):94-96.
[27]CHESLEY L C.The Concentrations of Proteases,Amylase,and Lipase in Certain Marine Fishes[J].Biological Bulletin,1934,66(14):133-144.
[28]寿建昕,沈文英,吴颖芳,等.温度对翘嘴红鲌消化酶活力的影响[J].水利渔业,2006,26(2):6-7,25.
[29]梅景良,马燕梅.温度和pH对黑鲷主要消化酶活性的影响[J].集美大学学报:自然科学版,2004,9(3):226-230.
[30]付新华,孙谧,孙世春.大菱鲆消化酶的活力[J].中国水产科学,2005,12(1):26-32.
[31]李芹,唐洪玉.中华倒刺鲃仔稚鱼期消化酶及碱性磷酸酶活性变化的研究[J].水生态学杂志,2010,3(5):82-85.
[32]沈文英,祝尧荣,钱科亮.温度和pH对澳洲宝石鱼消化酶活性的影响[J].大连水产学院学报,2006,21(2):189-192.
[33]杨元昊,周继术,吉红,等.温度对兰州鲇消化酶活性的影响[J].动物学杂志,2006,41(6):104-108.
[34]胡毅,潘鲁青.10种金属离子对三梭子蟹中肠腺消化酶活性的影响[J].热带海洋学报,2006,25(6):52-57.
[35]黄瑾,熊邦喜,陈洁,等.鱼类消化酶活性与其体长、体重和水质的相关性研究[J].水生态学杂志,2012,33(2):121-126.
[36]FANG L S,CHIOU S F.Effect of Salinity on the Activities of Digestive Proteases from the Tilapia Fish,Oreochromis Niloticus in Different Culture Environments[J].Comparative Biochemistry and Physiology,1989,93A(2):439-443.
[37]戴家银,郑微云.铜和锌离子对真鲷幼鱼组织酶活性的影响[J].环境科学,1998,l9(5):60-62.
[38]江信红,唐云明.黄鳝肠道蛋白酶的应用研究[J].西南师范大学学报:自然科学版,2006,31(4):143~147.
[39]祝云龙,李辉信,韩士群,等.氮磷对滩塘养殖水体及浮游植物的影响[J].江苏农业科学,2004(5):92-95.
[40]高梅,罗毅平,曹振东,等.饲料碳水化合物对南方鲇(Silurus meridionalis Chen)幼鱼消化酶活性的影响[J].西南师范大学学报:自然科学版,2006,31(2):119-123.
[41]邹师哲,王义强,张家圆.饲料中蛋白质、脂肪、碳水化合物对鲤消化酶的影响[J].上海水产大学学报,1998,7(1):69-74.
[42]NAGASE G.Contribution to the Physiology of Digestion in Tilapia Mossambica Peters:Digestive Enzymes and the Effects of Diets on Their Activity[J].Zeitschrift Fürvergleichende Physiologie,1964,49(3):270-284.
[43]李广丽,王义强.草鱼、鲤肠道,肝胰脏消化酶活性的初步研究[J].湛江水产学院学报,1994,14(1):34-40.