将荧光检测技术引入本科实验教学的实践与探索
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  • 英文篇名:Practice and exploration of introducing fluorescence detection technology into undergraduate experimental teaching
  • 作者:叶君 ; 熊犍 ; 吴胜龙 ; 何婉芬
  • 英文作者:YE Jun;XIONG Jian;WU Shenglong;HE Wanfen;School of Light Industry and Engineering, South China University of Technology;
  • 关键词:荧光检测技术 ; 快速检测 ; 教学实践 ; 创新能力
  • 英文关键词:fluorescence detection technology;;rapid detection;;teaching practice;;innovative ability
  • 中文刊名:SYJL
  • 英文刊名:Experimental Technology and Management
  • 机构:华南理工大学轻工科学与工程学院;
  • 出版日期:2019-03-25 10:53
  • 出版单位:实验技术与管理
  • 年:2019
  • 期:v.36;No.271
  • 基金:国家自然科学基金项目(31270617);; 制浆造纸工程国家重点实验室基金项目(2016C12);; 华南理工大学教研教改项目;华南理工大学第二批探索性教学项目(Y1150190)
  • 语种:中文;
  • 页:SYJL201903051
  • 页数:4
  • CN:03
  • ISSN:11-2034/T
  • 分类号:212-215
摘要
科学研究前沿和热点的荧光检测技术引入本科教学实践,合成了一种具有快速、高选择性和高灵敏度的新型荧光探针CMC/Tb(Ⅲ)复合物,用于对自来水中Mn~(2+)的快速检测。该实验的实施,对学生开拓知识视野、培养创新意识和创新技能等发挥了很好的作用。
        A new fluorescence probe CMC/Tb(III) complex with high sensitivity, high selectivity and rapidity is synthesized by introducing the fluorescence detection technology of the scientific research frontier and hotspot into undergraduate teaching practice, which is used for the rapid detection of Mn~(2+) in tap water. The implementation of this experiment has played a very good role in broadening students' horizons of knowledge, cultivating their innovative consciousness and skills.
引文
[1] KARUSO P. In the limelight part 2: GFP and the 2008 Nobel Prize in Chemistry[J]. Chemistry in Australia, 2009, 76(9):26-28.
    [2] FARIAS F M C D. Nobel Prize in Chemistry-2008. GFP: A shining tool to visualize life GFP: Uma ferramentabrilhante para a visualiza??o da vida[J]. Revista Virtual De Química, 2009, 1(1):2-8.
    [3] RODA A. Discovery and development of the green fluorescent protein, GFP: the 2008 Nobel Prize[J]. Analytical & Bioanalytical Chemistry, 2010, 396(5):1619-1622.
    [4] SHIMANE T, AIZAWA H, KOIKE T, et al. Oral cancer intraoperative detection by topically spraying a γ-glutamyl transpeptidase-activated fluorescent probe.[J]. Oral Oncology, 2016(54):e16-e18.
    [5] HEILEMANN M, VAN L S, SCHüTTPELZ M, et al. Subdiffraction-resolution fluorescence imaging with conventional fluorescent probes[J]. Angew Chem Int Ed Engl, 2010, 47(33):6172-6176.
    [6] LI Y, SHI L, QIN L X, et al. An OFF-ON fluorescent probe for Zn2+ based on a GFP-inspiredimidazolone derivative attached to a 1,10-phenanthroline moiety[J]. Chemical Communications, 2011, 47(15):4361-4363.
    [7] PISCITELLI A, PENNACCHI A, CICATIELLO P, et al. Rapid and ultrasensitive detection of active thrombin based on the Vmh2 hydrophobin fused to a Green Fluorescent Protein[J]. Biosensors & Bioelectronics, 2017 (87):816-822.
    [8] CHELLAPPA M M, DEY S, GAIKWAD S, et al. Rescue of a recombinant Newcastle disease virus strain R2B expressing green fluorescent protein[J]. Virus Genes, 2017, 53(3):1-8.
    [9] LI D, LU G, LEI C, et al. Sensitive detection of DNA methyltransferase activity based on supercharged fluorescent protein and template-free DNA polymerization[J]. Science China Chemistry, 2016, 59(7):809-815.
    [10] BR?M O, MESSINA F, El-ZOHRY A M, et al. Polychromatic femtosecond fluorescence studies of metal-polypyridine complexes in solution[J]. Chemical Physics, 2012, 393(1):51-57.
    [11] BOND T E H, SORENSON A E, SCHAEFFER P M. A green fluorescent protein-based assay for high-throughput ligand-binding studies of a mycobacterial biotin protein ligase[J]. Microbiological Research, 2017 (205):35.
    [12] LI Q Y, YE J, XIONG J, et al. Structures and high fluorescence of novel nanocomposites of sodium carboxymethyl cellulose/Tb(III) prepared at different pHs[J]. Polymer Composites, 2016, 38(S1):498-507.
    [13] TAN H, MA C, LI Q, et al. Functionalized Lanthanide Coordination Polymers Nanoparticles for Selective Sensing of Hydrogen Peroxide in Biological Fluids[J]. Analyst, 2014, 139(21):5516-5522.
    [14] MA Q, WANG Q. Lanthanide induced formation of novel luminescent alginate hydrogels and detection features[J].Carbohydr Polym, 2015 (133):19-23.
    [15] BORRAS S, GUITERAS J, COMPANYO R. Analysis of Sulfonamides in Animal Feeds by Liquid Chromatography with Fluorescence Detection[J]. J Agric Food Chem, 2011, 59 (10):5240-5247.
    [16] BOUCHARD M F, SAUVéS, BARBEAU B, et al. Intellectual Impairment in School-Age Children Exposed to Manganese from Drinking Water[J]. Environ HealthPerspect, 2011, 119 (1):138-143.
    [17] 胡小波. 本科实验教学中创新人才培养体系的研究与实践[J]. 实验技术与管理,2010, 27 (6):120-125.

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