杨树芽总黄酮的提取工艺及其抗氧化活性研究
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摘要
杨树芽是蜜蜂用于生产蜂胶的主要原料,尚未得到充分的开发利用。采用响应面实验设计方法,对杨树芽总黄酮的提取工艺进行优化,同时研究了杨树芽总黄酮提取物的体外抗氧化活性。实验结果表明,杨树芽总黄酮的最佳提取工艺条件为:提取时间2. 0 h、料液比1∶16 (g/m L)、乙醇体积分数85%,总黄酮提取率为13. 14%。体外抗氧化结果表明,杨树芽总黄酮提取物不仅能有效的清除自由基,对羟基自由基诱导的DNA氧化损伤也有明显的保护作用。
Poplar buds are the main raw materials used by bees to produce propolis and have not been fully exploited. In this paper,response surface methodology was used to optimize the extraction of total flavonoids from poplar buds. At the same time,the antioxidant activity of total flavonoids from poplar buds was researched in vitro. The results manifested that the optimum conditions of extracting total flavonoids from poplar bud were as follows: extraction duration,2 h; solid-liquid ratio,1∶ 16( g/m L); and ethanol concentration,85%; and the extraction efficiency was 13. 14%. The results of vitro antioxidant assays showed that extracts of total flavonoids from poplar buds could not only scavenge free radicals,but also have protective effects on hydroxyl radical-induced DNA oxidative damage. The results of this research provide the basis for the exploitation and utilization of poplar bud resources.
Poplar buds are the main raw materials used by bees to produce propolis and have not been fully exploited. In this paper,response surface methodology was used to optimize the extraction of total flavonoids from poplar buds. At the same time,the antioxidant activity of total flavonoids from poplar buds was researched in vitro. The results manifested that the optimum conditions of extracting total flavonoids from poplar bud were as follows: extraction duration,2 h; solid-liquid ratio,1∶ 16( g/m L); and ethanol concentration,85%; and the extraction efficiency was 13. 14%. The results of vitro antioxidant assays showed that extracts of total flavonoids from poplar buds could not only scavenge free radicals,but also have protective effects on hydroxyl radical-induced DNA oxidative damage. The results of this research provide the basis for the exploitation and utilization of poplar bud resources.
引文
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[23]王海燕,李睿,曾秀,等.响应面优化超声波提取桑叶槲皮素工艺[J].食品科学,2014,35(22):56-62.
[24] CHEN R,JIN C,TONG Z,et al. Optimization extraction,characterization and antioxidant activities of pectic polysaccharide from tangerine peels[J]. Carbohydrate Polymers,2016,136(3):187-97.
[25] BONDET V,BRAND-WILLIAMS W,BERSET C. Kinetics and mechanisms of antioxidant activity using the DPPH·free radical method[J]. LWT-Food Science and Technology,1997,30(6):609-615.
[26] ES-SAFI N E,KOLLMANN A,KHLIFIC S,et al.Antioxidative effect of compounds isolated from Globularia alypum L. structure-activity relationship[J].LWT-Food Science and Technology,2007,40(7):1246-1252.
[27]林芮昀.迷迭香精油的抗氧化性研究[J].化工管理,2018,1(3):82-83.
[28] JR M E,KANDASWAMI C,THEOHARIDES T C.The effects of plant flavonoids on mammalian cells:Implications for inflammation, heart disease, and cancer[J]. Pharmacological Reviews,2000,52(4):673-751.
[29]程妮.蜂蜜植物源鉴别新方法及其保肝活性研究[D].西安:西北大学,2012:54-58.
[30]图尔贡·艾尔肯,迪丽努尔·塔力甫,邵龙义,等.极干旱区大气PM 2. 5对质粒DNA的氧化性损伤研究[J].生态环境学报,2018,27(4):730-735.
[2] Wu Y W,Sun S Q,Zhao J,et al. Rapid discrimination of extracts of Chinese propolis and poplar buds by FT-IR and 2D IR correlation spectroscopy[J]. Journal of Molecular Structure,2008,s 883-884(1):48-54.
[3] WILSON M B,SPIVAK M,HEGEMAN A D,et al.Metabolomics reveals the origins of antimicrobial plant resins collected by Honey Bees[J]. Plos One,2013,8(10):e77512.
[4]胡浩,董捷,张红城,等.工蜂采集蜂胶的行为观察及胶源植物的研究[J].食品科学,2014,35(15):54-58.
[5] TORETI V C,SATO H H,PASTORE G M,et al.Recent progress of propolis for its biological and chemical compositions and its botanical origin[J]. Evid Based Complement Alternat Med,2013(1):69,73-90.
[6] BURDOCK G A. Review of the biological properties and toxicity of bee propolis(propolis)[J]. Food and Chemical Toxicology,1998,36(4):347-363.
[7]刘长龙,李海涛.杨树芽提取物体外抗氧化活性及抗DNA损伤作用[J].现代中药研究与实践,2014,28(2):37-40.
[8]范文,伊爱芹,陈媛梅.杨树芽和芽胶中化学成分的分析[J].林业工程学报,2009,23(1):32-35.
[9]曹炜,符军放,索志荣,等.蜂胶与杨树芽提取物成分的比较研究[J].食品与发酵工业,2007,33(7):162-166.
[10] KUS P,JERKOVIC I,JAKOVLJEVIC M,et al. Extraction of bioactive phenolics from black poplar(Populus nigra L.)buds by supercritical CO2and its optimization by response surface methodology[J]. Journal of Pharmaceutical and Biomedical Analysis, 2018,152:128-136.
[11] MUNOZ C C. Usos medicinales del chopo negro(Populus nigra L.)[J]. Medicina Naturista,2013,7:99-115.
[12] DUDONNE S,POUPARD P,COUTIERE P,et al.Phenolic composition and antioxidant properties of Poplar Bud(Populus nigra)Extract:Individual antioxidant contribution of phenolics and transcriptional effect on skin aging[J]. Journal of Agricultural and Food Chemistry,2011,59(9):4527-4536.
[13] BURDOCK G A. Review of the biological properties and toxicity of bee propolis(propolis)[J]. Food&Chemical Toxicology,1998,36(4):347-363.
[14]李骄,郑光耀,李海涛.杨树芽提取物对实验性胃溃疡的防治作用[J].现代中药研究与实践,2012,26(3):36-39.
[15]孙丽萍,田文礼,朱晓丽,等.聚酰胺-UV法检测蜂花粉总黄酮的研究[J].中国蜂业,2006,57(8):8-10.
[16] HAOAN ZHAO,NI CHENG,LIANG He,et al. Antioxidant and hepatoprotective effects of A. cerana honey against acute alcohol-induced liver damage in mice[J]. Food Research International,2017,101:35-44.
[17]马士巧,马海乐.黑蜂蜂胶不同极性提取物的体外抗氧化活性[J].中国食品学报,2016,16(8):54-55.
[18] ZHANG X,GUO Y,WANG C,et al. Phenolic compounds from origanum vulgare and their antioxidant and antiviral activities[J]. Food Chemistry,2014,152(2):300-306.
[19] RAJAPAKSE N,MENDIS E,JUND W K,et al. Purification of a radical scavenging peptide from fermented mussel sauce andits antioxidant properties[J]. Food Research International,2005,38(2):175-182.
[20] AMADOU I,LE G W,AMZA T,et al. Purification and characterization of foxtail millet-derived peptides with antioxidant and antimicrobial activities[J]. Food Research International,2013,51(1):422-428.
[21]仇燕,仇彦博.柚皮总黄酮超声波辅助提取工艺的研究[J].河北科技大学学报,2010,31(4):330-333.
[22]王欣,王强,徐国钧,等.杨属植物化学成分和药理作用的研究进展[J].天然产物研究与开发,1999,11(1):65-74.
[23]王海燕,李睿,曾秀,等.响应面优化超声波提取桑叶槲皮素工艺[J].食品科学,2014,35(22):56-62.
[24] CHEN R,JIN C,TONG Z,et al. Optimization extraction,characterization and antioxidant activities of pectic polysaccharide from tangerine peels[J]. Carbohydrate Polymers,2016,136(3):187-97.
[25] BONDET V,BRAND-WILLIAMS W,BERSET C. Kinetics and mechanisms of antioxidant activity using the DPPH·free radical method[J]. LWT-Food Science and Technology,1997,30(6):609-615.
[26] ES-SAFI N E,KOLLMANN A,KHLIFIC S,et al.Antioxidative effect of compounds isolated from Globularia alypum L. structure-activity relationship[J].LWT-Food Science and Technology,2007,40(7):1246-1252.
[27]林芮昀.迷迭香精油的抗氧化性研究[J].化工管理,2018,1(3):82-83.
[28] JR M E,KANDASWAMI C,THEOHARIDES T C.The effects of plant flavonoids on mammalian cells:Implications for inflammation, heart disease, and cancer[J]. Pharmacological Reviews,2000,52(4):673-751.
[29]程妮.蜂蜜植物源鉴别新方法及其保肝活性研究[D].西安:西北大学,2012:54-58.
[30]图尔贡·艾尔肯,迪丽努尔·塔力甫,邵龙义,等.极干旱区大气PM 2. 5对质粒DNA的氧化性损伤研究[J].生态环境学报,2018,27(4):730-735.